Q5F3A6 (OTU1_CHICK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin thioesterase OTU1
EC=3.4.19.12

Gene names

Name:	YOD1
ORF Names:	RCJMB04_24h21

Organism

Gallus gallus (Chicken) [Reference proteome]

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Sauropsida › Sauria › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	302 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Hydrolase that can remove conjugated ubiquitin from proteins and participates in endoplasmic reticulum-associated degradation (ERAD) for misfolded lumenal proteins. May act by triming the ubiquitin chain on the associated substrate to facilitate their threading through the VCP/p97 pore. Ubiquitin moieties on substrates may present a steric impediment to the threading process when the substrate is transferred to the VCP pore and threaded through VCP's axial channel By similarity.
Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Sequence similarities	Contains 1 C2H2-type zinc finger. Contains 1 OTU domain.

Ontologies

Keywords
Biological process	Ubl conjugation pathway Unfolded protein response
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Protease Thiol protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ER-associated protein catabolic process Inferred from sequence or structural similarity. Source: UniProtKB cellular amino acid metabolic process Inferred from sequence or structural similarity. Source: UniProtKB endoplasmic reticulum unfolded protein response Inferred from sequence or structural similarity. Source: UniProtKB protein K48-linked deubiquitination Inferred from sequence or structural similarity. Source: UniProtKB protein K63-linked deubiquitination Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular_function	ubiquitin-specific protease activity Inferred from sequence or structural similarity. Source: UniProtKB zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 302	302	Ubiquitin thioesterase OTU1		PRO_0000282359
Regions
	Domain	103 – 228	126	OTU
	Zinc finger	272 – 296	25	C2H2-type
	Region	5 – 83	79	UBX-like
Sites
	Active site	111	1	By similarity
	Active site	114	1	Nucleophile By similarity
	Active site	296	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5F3A6 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: 6ABA3C65C985248C	FASTA	302	33,384
	10 20 30 40 50 60 MLRLRCKARS GTQPLPGLTA HSRLRDMQAA LAALTGVPAP AQRLLLGFPP RSLDLSDGER 70 80 90 100 110 120 RLGELGIHSG DTLIVEEDTS KPSAGSPVVA KRTMAVREAV PVLARRVVPA DNSCLFTSVY 130 140 150 160 170 180 YVVEGGVYDP GCAPEMRSLI AQIVASDPEA YCEAVLGKTN REYCEWIRRE ETWGGAIEVS 190 200 210 220 230 240 ILSKFYQCEI CVVDTQTVRI DRFGEDAGYT KRVLLIYDGI HYDPLERKIP DSDVPPQTIF 250 260 270 280 290 300 STTDDVVLAQ ALELADEARR KRQFTDVNRF TLRCMVCQKG LTGQVEAREH AKETGHTNFG EV

References

[1]

"Full-length cDNAs from chicken bursal lymphocytes to facilitate gene function analysis." Caldwell R.B., Kierzek A.M., Arakawa H., Bezzubov Y., Zaim J., Fiedler P., Kutter S., Blagodatski A., Kostovska D., Koter M., Plachy J., Carninci P., Hayashizaki Y., Buerstedde J.-M. Genome Biol. 6:R6.1-R6.9(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: CB. Tissue: Bursa of Fabricius.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ851744 mRNA. Translation: CAH65378.1.
IPI	IPI00595334.
RefSeq	NP_001026670.1. NM_001031499.1.
UniGene	Gga.42722.
3D structure databases
ProteinModelPortal	Q5F3A6.
ModBase	Search...
Protein-protein interaction databases
STRING	9031.ENSGALP00000039556.
Protein family/group databases
MEROPS	C85.007.
Proteomic databases
PaxDb	Q5F3A6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	428266.
KEGG	gga:428266.
Organism-specific databases
CTD	55432.
Phylogenomic databases
eggNOG	COG5539.
HOGENOM	HOG000193461.
HOVERGEN	HBG097006.
InParanoid	Q5F3A6.
KO	K13719.
OrthoDB	EOG4GF3FR.
Family and domain databases
InterPro	IPR003323. OTU. IPR007087. Znf_C2H2. IPR015880. Znf_C2H2-like. [Graphical view]
Pfam	PF02338. OTU. 1 hit. [Graphical view]
SMART	SM00355. ZnF_C2H2. 1 hit. [Graphical view]
PROSITE	PS50802. OTU. 1 hit. PS00299. UBIQUITIN_1. False negative. PS50053. UBIQUITIN_2. False negative. PS00028. ZINC_FINGER_C2H2_1. 1 hit. PS50157. ZINC_FINGER_C2H2_2. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	20829307.

Entry information

Entry name

OTU1_CHICK

Accession

Primary (citable) accession number: Q5F3A6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 3, 2007
Last sequence update:	March 15, 2005
Last modified:	April 3, 2013
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program