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Q5F3A6

- OTU1_CHICK

UniProt

Q5F3A6 - OTU1_CHICK

Protein

Ubiquitin thioesterase OTU1

Gene

YOD1

Organism
Gallus gallus (Chicken)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 55 (01 Oct 2014)
      Sequence version 1 (15 Mar 2005)
      Previous versions | rss
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    Functioni

    Hydrolase that can remove conjugated ubiquitin from proteins and participates in endoplasmic reticulum-associated degradation (ERAD) for misfolded lumenal proteins. May act by triming the ubiquitin chain on the associated substrate to facilitate their threading through the VCP/p97 pore. Ubiquitin moieties on substrates may present a steric impediment to the threading process when the substrate is transferred to the VCP pore and threaded through VCP's axial channel. Mediates deubiquitination of 'Lys-27'-, 'Lys-29'- and 'Lys-33'-linked polyubiquitin chains. Also able to hydrolyze 'Lys-11'-linked ubiquitin chains. Cleaves both polyubiquitin and di-ubiquitin By similarity.By similarity

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei111 – 1111By similarity
    Active sitei114 – 1141NucleophileBy similarity
    Binding sitei220 – 2201Substrate; via carbonyl oxygenBy similarity
    Active sitei221 – 2211By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri272 – 29625C2H2-typeAdd
    BLAST

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. ubiquitin-specific protease activity Source: UniProtKB

    GO - Biological processi

    1. cellular amino acid metabolic process Source: UniProtKB
    2. endoplasmic reticulum unfolded protein response Source: UniProtKB
    3. ER-associated ubiquitin-dependent protein catabolic process Source: UniProtKB
    4. protein K11-linked deubiquitination Source: UniProtKB
    5. protein K27-linked deubiquitination Source: UniProtKB
    6. protein K29-linked deubiquitination Source: UniProtKB
    7. protein K33-linked deubiquitination Source: UniProtKB
    8. protein K48-linked deubiquitination Source: UniProtKB
    9. protein K63-linked deubiquitination Source: UniProtKB

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Ubl conjugation pathway, Unfolded protein response

    Keywords - Ligandi

    Metal-binding, Zinc

    Protein family/group databases

    MEROPSiC85.007.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin thioesterase OTU1 (EC:3.4.19.12)
    Gene namesi
    Name:YOD1
    ORF Names:RCJMB04_24h21
    OrganismiGallus gallus (Chicken)
    Taxonomic identifieri9031 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus
    ProteomesiUP000000539: Unplaced

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 302302Ubiquitin thioesterase OTU1PRO_0000282359Add
    BLAST

    Proteomic databases

    PaxDbiQ5F3A6.

    Interactioni

    Protein-protein interaction databases

    STRINGi9031.ENSGALP00000039556.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5F3A6.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini103 – 228126OTUPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni5 – 8379UBX-likeAdd
    BLAST
    Regioni108 – 1147Cys-loopBy similarity
    Regioni167 – 17711Variable-loopBy similarityAdd
    BLAST
    Regioni217 – 2215His-loopBy similarity
    Regioni245 – 2506S2 siteBy similarity

    Domaini

    The C2H2-type zinc finger mediates specificity for 'Lys-27'-, 'Lys-29'- and 'Lys-33'-linked polyubiquitin chains but not for 'Lys-11'-linked ubiquitin chains. Selectivity for 'Lys-11'-linked ubiquitin chains is provided by recognition of the sequence surrounding 'Lys-11' in ubiquitin. The S2 site region provides specificity for longer 'Lys-11'-linked ubiquitin chains By similarity.By similarity

    Sequence similaritiesi

    Contains 1 C2H2-type zinc finger.Curated
    Contains 1 OTU domain.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri272 – 29625C2H2-typeAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5539.
    HOGENOMiHOG000193461.
    HOVERGENiHBG097006.
    InParanoidiQ5F3A6.
    KOiK13719.
    PhylomeDBiQ5F3A6.

    Family and domain databases

    InterProiIPR003323. OTU.
    IPR029071. Ubiquitin-rel_dom.
    IPR007087. Znf_C2H2.
    IPR015880. Znf_C2H2-like.
    [Graphical view]
    PfamiPF02338. OTU. 1 hit.
    [Graphical view]
    SMARTiSM00355. ZnF_C2H2. 1 hit.
    [Graphical view]
    SUPFAMiSSF54236. SSF54236. 1 hit.
    PROSITEiPS50802. OTU. 1 hit.
    PS00028. ZINC_FINGER_C2H2_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q5F3A6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLRLRCKARS GTQPLPGLTA HSRLRDMQAA LAALTGVPAP AQRLLLGFPP    50
    RSLDLSDGER RLGELGIHSG DTLIVEEDTS KPSAGSPVVA KRTMAVREAV 100
    PVLARRVVPA DNSCLFTSVY YVVEGGVYDP GCAPEMRSLI AQIVASDPEA 150
    YCEAVLGKTN REYCEWIRRE ETWGGAIEVS ILSKFYQCEI CVVDTQTVRI 200
    DRFGEDAGYT KRVLLIYDGI HYDPLERKIP DSDVPPQTIF STTDDVVLAQ 250
    ALELADEARR KRQFTDVNRF TLRCMVCQKG LTGQVEAREH AKETGHTNFG 300
    EV 302
    Length:302
    Mass (Da):33,384
    Last modified:March 15, 2005 - v1
    Checksum:i6ABA3C65C985248C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ851744 mRNA. Translation: CAH65378.1.
    RefSeqiNP_001026670.1. NM_001031499.1.
    UniGeneiGga.42722.

    Genome annotation databases

    GeneIDi428266.
    KEGGigga:428266.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ851744 mRNA. Translation: CAH65378.1 .
    RefSeqi NP_001026670.1. NM_001031499.1.
    UniGenei Gga.42722.

    3D structure databases

    ProteinModelPortali Q5F3A6.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9031.ENSGALP00000039556.

    Protein family/group databases

    MEROPSi C85.007.

    Proteomic databases

    PaxDbi Q5F3A6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 428266.
    KEGGi gga:428266.

    Organism-specific databases

    CTDi 55432.

    Phylogenomic databases

    eggNOGi COG5539.
    HOGENOMi HOG000193461.
    HOVERGENi HBG097006.
    InParanoidi Q5F3A6.
    KOi K13719.
    PhylomeDBi Q5F3A6.

    Miscellaneous databases

    NextBioi 20829307.
    PROi Q5F3A6.

    Family and domain databases

    InterProi IPR003323. OTU.
    IPR029071. Ubiquitin-rel_dom.
    IPR007087. Znf_C2H2.
    IPR015880. Znf_C2H2-like.
    [Graphical view ]
    Pfami PF02338. OTU. 1 hit.
    [Graphical view ]
    SMARTi SM00355. ZnF_C2H2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54236. SSF54236. 1 hit.
    PROSITEi PS50802. OTU. 1 hit.
    PS00028. ZINC_FINGER_C2H2_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: CB.
      Tissue: Bursa of Fabricius.

    Entry informationi

    Entry nameiOTU1_CHICK
    AccessioniPrimary (citable) accession number: Q5F3A6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 3, 2007
    Last sequence update: March 15, 2005
    Last modified: October 1, 2014
    This is version 55 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3