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Q5F3A6 (OTU1_CHICK) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 54. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Ubiquitin thioesterase OTU1

EC=3.4.19.12
Gene names
Name:YOD1
ORF Names:RCJMB04_24h21
OrganismGallus gallus (Chicken) [Reference proteome]
Taxonomic identifier9031 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus

Protein attributes

Sequence length302 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Hydrolase that can remove conjugated ubiquitin from proteins and participates in endoplasmic reticulum-associated degradation (ERAD) for misfolded lumenal proteins. May act by triming the ubiquitin chain on the associated substrate to facilitate their threading through the VCP/p97 pore. Ubiquitin moieties on substrates may present a steric impediment to the threading process when the substrate is transferred to the VCP pore and threaded through VCP's axial channel. Mediates deubiquitination of 'Lys-27'-, 'Lys-29'- and 'Lys-33'-linked polyubiquitin chains. Also able to hydrolyze 'Lys-11'-linked ubiquitin chains. Cleaves both polyubiquitin and di-ubiquitin By similarity.

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Domain

The C2H2-type zinc finger mediates specificity for 'Lys-27'-, 'Lys-29'- and 'Lys-33'-linked polyubiquitin chains but not for 'Lys-11'-linked ubiquitin chains. Selectivity for 'Lys-11'-linked ubiquitin chains is provided by recognition of the sequence surrounding 'Lys-11' in ubiquitin. The S2 site region provides specificity for longer 'Lys-11'-linked ubiquitin chains By similarity.

Sequence similarities

Contains 1 C2H2-type zinc finger.

Contains 1 OTU domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 302302Ubiquitin thioesterase OTU1
PRO_0000282359

Regions

Domain103 – 228126OTU
Zinc finger272 – 29625C2H2-type
Region5 – 8379UBX-like
Region108 – 1147Cys-loop By similarity
Region167 – 17711Variable-loop By similarity
Region217 – 2215His-loop By similarity
Region245 – 2506S2 site By similarity

Sites

Active site1111 By similarity
Active site1141Nucleophile By similarity
Active site2211 By similarity
Binding site2201Substrate; via carbonyl oxygen By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5F3A6 [UniParc].

Last modified March 15, 2005. Version 1.
Checksum: 6ABA3C65C985248C

FASTA30233,384
        10         20         30         40         50         60 
MLRLRCKARS GTQPLPGLTA HSRLRDMQAA LAALTGVPAP AQRLLLGFPP RSLDLSDGER 

        70         80         90        100        110        120 
RLGELGIHSG DTLIVEEDTS KPSAGSPVVA KRTMAVREAV PVLARRVVPA DNSCLFTSVY 

       130        140        150        160        170        180 
YVVEGGVYDP GCAPEMRSLI AQIVASDPEA YCEAVLGKTN REYCEWIRRE ETWGGAIEVS 

       190        200        210        220        230        240 
ILSKFYQCEI CVVDTQTVRI DRFGEDAGYT KRVLLIYDGI HYDPLERKIP DSDVPPQTIF 

       250        260        270        280        290        300 
STTDDVVLAQ ALELADEARR KRQFTDVNRF TLRCMVCQKG LTGQVEAREH AKETGHTNFG 


EV 

« Hide

References

[1]"Full-length cDNAs from chicken bursal lymphocytes to facilitate gene function analysis."
Caldwell R.B., Kierzek A.M., Arakawa H., Bezzubov Y., Zaim J., Fiedler P., Kutter S., Blagodatski A., Kostovska D., Koter M., Plachy J., Carninci P., Hayashizaki Y., Buerstedde J.-M.
Genome Biol. 6:R6.1-R6.9(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: CB.
Tissue: Bursa of Fabricius.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ851744 mRNA. Translation: CAH65378.1.
RefSeqNP_001026670.1. NM_001031499.1.
UniGeneGga.42722.

3D structure databases

ProteinModelPortalQ5F3A6.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9031.ENSGALP00000039556.

Protein family/group databases

MEROPSC85.007.

Proteomic databases

PaxDbQ5F3A6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID428266.
KEGGgga:428266.

Organism-specific databases

CTD55432.

Phylogenomic databases

eggNOGCOG5539.
HOGENOMHOG000193461.
HOVERGENHBG097006.
InParanoidQ5F3A6.
KOK13719.
PhylomeDBQ5F3A6.

Family and domain databases

InterProIPR003323. OTU.
IPR029071. Ubiquitin-rel_dom.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
[Graphical view]
PfamPF02338. OTU. 1 hit.
[Graphical view]
SMARTSM00355. ZnF_C2H2. 1 hit.
[Graphical view]
SUPFAMSSF54236. SSF54236. 1 hit.
PROSITEPS50802. OTU. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20829307.
PROQ5F3A6.

Entry information

Entry nameOTU1_CHICK
AccessionPrimary (citable) accession number: Q5F3A6
Entry history
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: March 15, 2005
Last modified: June 11, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families