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Protein

Serine/threonine-protein kinase TAO1

Gene

Taok1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Serine/threonine-protein kinase involved in various processes such as p38/MAPK14 stress-activated MAPK cascade, DNA damage response and regulation of cytoskeleton stability. Phosphorylates MAP2K3, MAP2K6 and MARK2. Acts as an activator of the p38/MAPK14 stress-activated MAPK cascade by mediating phosphorylation and subsequent activation of the upstream MAP2K3 and MAP2K6 kinases. Involved in G-protein coupled receptor signaling to p38/MAPK14. In response to DNA damage, involved in the G2/M transition DNA damage checkpoint by activating the p38/MAPK14 stress-activated MAPK cascade, probably by mediating phosphorylation of MAP2K3 and MAP2K6. Acts as a regulator of cytoskeleton stability by phosphorylating 'Thr-208' of MARK2, leading to activate MARK2 kinase activity and subsequent phosphorylation and detachment of MAPT/TAU from microtubules. Also acts as a regulator of apoptosis: regulates apoptotic morphological changes, including cell contraction, membrane blebbing and apoptotic bodies formation via activation of the MAPK8/JNK cascade (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Serine/threonine-protein kinase activity is inhibited by SPRED1.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei57 – 571ATPPROSITE-ProRule annotation
Active sitei151 – 1511Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi34 – 429ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. MAP kinase kinase kinase activity Source: GO_Central
  3. protein serine/threonine kinase activity Source: UniProtKB

GO - Biological processi

  1. activation of MAPKK activity Source: GOC
  2. cellular response to DNA damage stimulus Source: UniProtKB
  3. DNA repair Source: UniProtKB-KW
  4. execution phase of apoptosis Source: UniProtKB
  5. G2 DNA damage checkpoint Source: UniProtKB
  6. intracellular signal transduction Source: GO_Central
  7. MAPK cascade Source: GOC
  8. positive regulation of JNK cascade Source: UniProtKB
  9. positive regulation of stress-activated MAPK cascade Source: UniProtKB
  10. regulation of cytoskeleton organization Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Apoptosis, DNA damage, DNA repair

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_306375. Mitotic Prometaphase.
REACT_321346. Separation of Sister Chromatids.
REACT_329805. Resolution of Sister Chromatid Cohesion.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase TAO1 (EC:2.7.11.1)
Alternative name(s):
Thousand and one amino acid protein 1
Gene namesi
Name:Taok1
Synonyms:Kiaa1361
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1914490. Taok1.

Subcellular locationi

  1. Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: GO_Central
  2. extracellular vesicular exosome Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10011001Serine/threonine-protein kinase TAO1PRO_0000086729Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei9 – 91PhosphoserineBy similarity
Modified residuei421 – 4211Phosphoserine1 Publication
Modified residuei445 – 4451PhosphoserineBy similarity
Modified residuei669 – 6691PhosphothreonineBy similarity
Modified residuei965 – 9651PhosphoserineBy similarity

Post-translational modificationi

Proteolytically processed by caspase-3 (CASP3).
Autophosphorylated (By similarity). Phosphorylated by ATM in response to DNA damage. Phosphorylated by LRRK2.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ5F2E8.
PaxDbiQ5F2E8.
PRIDEiQ5F2E8.

PTM databases

PhosphoSiteiQ5F2E8.

Expressioni

Gene expression databases

BgeeiQ5F2E8.
CleanExiMM_TAOK1.
ExpressionAtlasiQ5F2E8. baseline and differential.
GenevestigatoriQ5F2E8.

Interactioni

Subunit structurei

Self-associates. Interacts with MAP2K3. Interacts with SPRED1 and TESK1. Interacts with MAP3K7 (By similarity).By similarity

Protein-protein interaction databases

BioGridi229824. 2 interactions.
IntActiQ5F2E8. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ5F2E8.
SMRiQ5F2E8. Positions 12-376.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini28 – 281254Protein kinasePROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili458 – 651194Sequence AnalysisAdd
BLAST
Coiled coili754 – 877124Sequence AnalysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi330 – 3345Poly-Glu

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00760000118848.
HOGENOMiHOG000236358.
HOVERGENiHBG088996.
InParanoidiQ5F2E8.
KOiK04429.
OMAiAGPHWGH.
OrthoDBiEOG74XS5S.
PhylomeDBiQ5F2E8.
TreeFamiTF351444.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5F2E8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPSTNRAGSL KDPEIAELFF KEDPEKLFTD LREIGHGSFG AVYFARDVRT
60 70 80 90 100
NEVVAIKKMS YSGKQSTEKW QDIIKEVKFL QRIKHPNSIE YKGCYLREHT
110 120 130 140 150
AWLVMEYCLG SASDLLEVHK KPLQEVEIAA ITHGALQGLA YLHSHTMIHR
160 170 180 190 200
DIKAGNILLT EPGQVKLADF GSASMASPAN SFVGTPYWMA PEVILAMDEG
210 220 230 240 250
QYDGKVDVWS LGITCIELAE RKPPLFNMNA MSALYHIAQN ESPTLQSNEW
260 270 280 290 300
SDYFRNFVDS CLQKIPQDRP TSEELLKHMF VLRERPETVL IDLIQRTKDA
310 320 330 340 350
VRELDNLQYR KMKKLLFQEA HNGPAVEAQE EEEEQDHGVG RTGTVNSVGS
360 370 380 390 400
NQSIPSMSIS ASSQSSSVNS LPDASDDKSE LDMMEGDHTV MSNSSVIHLK
410 420 430 440 450
PEEENYQEEG DPRTRASDPQ SPPQVSRHKS HYRNREHFAT IRTASLVTRQ
460 470 480 490 500
MQEHEQDSEL REQMSGYKRM RRQHQKQLMT LENKLKAEMD EHRLRLDKDL
510 520 530 540 550
ETQRNNFAAE MEKLIKKHQA AMEKEAKVMA NEEKKFQQHI QAQQKKELNS
560 570 580 590 600
FLESQKREYK LRKEQLKEEL NENQSTPKKE KQEWLSKQKE NIQHFQAEEE
610 620 630 640 650
ANLLRRQRQY LELECRRFKR RMLLGRHNLE QDLVREELNK RQTQKDLEHA
660 670 680 690 700
MLLRQHESMQ ELEFRHLNTI QKMRCELIRL QHQTELTNQL EYNKRREREL
710 720 730 740 750
RRKHVMEVRQ QPKSLKSKEL QIKKQFQDTC KIQTRQYKAL RNHLLETTPK
760 770 780 790 800
SEHKAVLKRL KEEQTRKLAI LAEQYDHSIN EMLSTQALRL DEAQEAECQV
810 820 830 840 850
LKMQLQQELE LLNAYQSKIK MQAEAQHDRE LRELEQRVSL RRALLEQKIE
860 870 880 890 900
EEMLALQNER TERIRSLLER QAREIEAFDS ESMRLGFSNM VLSNLSPEAF
910 920 930 940 950
SHSYPGASSW SHNPTGGPGP HWGHPMGGTP QAWGHPMQGG PQPWGHPSGP
960 970 980 990 1000
MQGVPRGSSM GVRNSPQALR RTASGGRTEQ GMSRSTSVTS QISNGSHMSY

T
Length:1,001
Mass (Da):116,050
Last modified:March 15, 2005 - v1
Checksum:i59DF437EAB8127C2
GO

Sequence cautioni

The sequence AAH16522.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence BAD32434.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL591136 Genomic DNA. Translation: CAI51877.1.
BC016522 mRNA. Translation: AAH16522.1. Different initiation.
BC034906 mRNA. Translation: AAH34906.1.
AK173156 mRNA. Translation: BAD32434.1. Different initiation.
CCDSiCCDS36235.1.
RefSeqiNP_659074.2. NM_144825.2.
XP_006533021.1. XM_006532958.2.
XP_006533022.1. XM_006532959.2.
UniGeneiMm.340436.

Genome annotation databases

EnsembliENSMUST00000017435; ENSMUSP00000017435; ENSMUSG00000017291.
ENSMUST00000058496; ENSMUSP00000055470; ENSMUSG00000017291.
GeneIDi216965.
KEGGimmu:216965.
UCSCiuc007khc.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL591136 Genomic DNA. Translation: CAI51877.1.
BC016522 mRNA. Translation: AAH16522.1. Different initiation.
BC034906 mRNA. Translation: AAH34906.1.
AK173156 mRNA. Translation: BAD32434.1. Different initiation.
CCDSiCCDS36235.1.
RefSeqiNP_659074.2. NM_144825.2.
XP_006533021.1. XM_006532958.2.
XP_006533022.1. XM_006532959.2.
UniGeneiMm.340436.

3D structure databases

ProteinModelPortaliQ5F2E8.
SMRiQ5F2E8. Positions 12-376.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi229824. 2 interactions.
IntActiQ5F2E8. 1 interaction.

PTM databases

PhosphoSiteiQ5F2E8.

Proteomic databases

MaxQBiQ5F2E8.
PaxDbiQ5F2E8.
PRIDEiQ5F2E8.

Protocols and materials databases

DNASUi216965.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000017435; ENSMUSP00000017435; ENSMUSG00000017291.
ENSMUST00000058496; ENSMUSP00000055470; ENSMUSG00000017291.
GeneIDi216965.
KEGGimmu:216965.
UCSCiuc007khc.1. mouse.

Organism-specific databases

CTDi57551.
MGIiMGI:1914490. Taok1.
RougeiSearch...

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00760000118848.
HOGENOMiHOG000236358.
HOVERGENiHBG088996.
InParanoidiQ5F2E8.
KOiK04429.
OMAiAGPHWGH.
OrthoDBiEOG74XS5S.
PhylomeDBiQ5F2E8.
TreeFamiTF351444.

Enzyme and pathway databases

ReactomeiREACT_306375. Mitotic Prometaphase.
REACT_321346. Separation of Sister Chromatids.
REACT_329805. Resolution of Sister Chromatid Cohesion.

Miscellaneous databases

ChiTaRSiTaok1. mouse.
NextBioi375476.
PROiQ5F2E8.
SOURCEiSearch...

Gene expression databases

BgeeiQ5F2E8.
CleanExiMM_TAOK1.
ExpressionAtlasiQ5F2E8. baseline and differential.
GenevestigatoriQ5F2E8.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 437-1001.
    Strain: FVB/N.
    Tissue: Mammary gland and Thymus.
  3. "Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H., Nagase T., Ohara O., Koga H.
    DNA Res. 11:205-218(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 657-1001.
    Tissue: Brain.
  4. "Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
    Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
    Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.
  5. "Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations."
    Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M.
    Mol. Cell. Proteomics 6:283-293(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain cortex.
  6. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-421, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiTAOK1_MOUSE
AccessioniPrimary (citable) accession number: Q5F2E8
Secondary accession number(s): Q69ZL2, Q8JZX2, Q91VG7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: March 15, 2005
Last modified: April 1, 2015
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.