Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Spermatid maturation protein 1

Gene

Spem1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Required for proper cytoplasm removal during spermatogenesis.1 Publication

GO - Biological processi

  • flagellated sperm motility Source: MGI
  • spermatogenesis Source: MGI
  • sperm individualization Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, Spermatogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
Spermatid maturation protein 1
Gene namesi
Name:Spem1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1921538. Spem1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei29 – 49HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

  • cytoplasm Source: MGI
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice are completely infertile because of deformed sperm characterized by a bent head wrapped around by the neck and the middle piece of the tail. Lack of Spem1 causes failure of the cytoplasm to become loose and detach from the head and the neck region of the developing spermatozoa.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003078071 – 310Spermatid maturation protein 1Add BLAST310

Proteomic databases

PaxDbiQ5F289.
PRIDEiQ5F289.

PTM databases

iPTMnetiQ5F289.
PhosphoSitePlusiQ5F289.

Expressioni

Tissue specificityi

Testis-specific. Exclusively present in cytoplasm of steps 14-16 elongated spermatids (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000041165.
CleanExiMM_SPEM1.
GenevisibleiQ5F289. MM.

Interactioni

Protein-protein interaction databases

BioGridi216637. 1 interactor.
STRINGi10090.ENSMUSP00000037500.

Structurei

3D structure databases

ProteinModelPortaliQ5F289.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili262 – 286Sequence analysisAdd BLAST25

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi208 – 213Poly-Pro6

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410J2CE. Eukaryota.
ENOG41118KW. LUCA.
GeneTreeiENSGT00510000049389.
HOGENOMiHOG000154380.
HOVERGENiHBG108506.
InParanoidiQ5F289.
OMAiAWAPDTD.
OrthoDBiEOG091G0L7A.
PhylomeDBiQ5F289.
TreeFamiTF338031.

Family and domain databases

InterProiIPR031368. SPEM1_N.
[Graphical view]
PfamiPF15670. Spem1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5F289-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAMAERPRPG WASYQNPNIN NCQDMGNSIL LLLGLIVCIN IGINLVTLLW
60 70 80 90 100
SRIRILLHRM FHIICEKETS TSLGKLVQPL RKQTYPKVHL RCTMDPVKMT
110 120 130 140 150
VTPPPTRRRY RHRAPPSRRA RCPIAWAPDT DDEKPPHQHP AICSRHWNDS
160 170 180 190 200
KDWEGFQSMQ EVWEPWTQDG LEQPPQTIRF QPAVEARPLK SEIRSDQGLE
210 220 230 240 250
AYVYTVNPPP PSPEALSHKN NAAGSGGCVE GEQAQGQPVS PSFLGPANVP
260 270 280 290 300
EIPRRHSSGR IVYDARDVRR RLRELTQEVE ALSHCYPLVS GSSAAEGTGK
310
DWVYRPLKGR
Length:310
Mass (Da):35,062
Last modified:March 15, 2005 - v1
Checksum:iF5AC1EB7A91864BC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti52R → Q in BAB24854 (PubMed:16141072).Curated1
Sequence conflicti213P → L in AAI00506 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF120626 mRNA. Translation: ABO33756.1.
AK007080 mRNA. Translation: BAB24854.1.
AL603707 Genomic DNA. Translation: CAI51963.1.
BC100505 mRNA. Translation: AAI00506.1.
CCDSiCCDS36199.1.
RefSeqiNP_083131.1. NM_028855.1.
UniGeneiMm.159159.

Genome annotation databases

EnsembliENSMUST00000045771; ENSMUSP00000037500; ENSMUSG00000041165.
GeneIDi74288.
KEGGimmu:74288.
UCSCiuc007jrv.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF120626 mRNA. Translation: ABO33756.1.
AK007080 mRNA. Translation: BAB24854.1.
AL603707 Genomic DNA. Translation: CAI51963.1.
BC100505 mRNA. Translation: AAI00506.1.
CCDSiCCDS36199.1.
RefSeqiNP_083131.1. NM_028855.1.
UniGeneiMm.159159.

3D structure databases

ProteinModelPortaliQ5F289.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi216637. 1 interactor.
STRINGi10090.ENSMUSP00000037500.

PTM databases

iPTMnetiQ5F289.
PhosphoSitePlusiQ5F289.

Proteomic databases

PaxDbiQ5F289.
PRIDEiQ5F289.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000045771; ENSMUSP00000037500; ENSMUSG00000041165.
GeneIDi74288.
KEGGimmu:74288.
UCSCiuc007jrv.1. mouse.

Organism-specific databases

CTDi374768.
MGIiMGI:1921538. Spem1.

Phylogenomic databases

eggNOGiENOG410J2CE. Eukaryota.
ENOG41118KW. LUCA.
GeneTreeiENSGT00510000049389.
HOGENOMiHOG000154380.
HOVERGENiHBG108506.
InParanoidiQ5F289.
OMAiAWAPDTD.
OrthoDBiEOG091G0L7A.
PhylomeDBiQ5F289.
TreeFamiTF338031.

Miscellaneous databases

PROiQ5F289.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000041165.
CleanExiMM_SPEM1.
GenevisibleiQ5F289. MM.

Family and domain databases

InterProiIPR031368. SPEM1_N.
[Graphical view]
PfamiPF15670. Spem1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSPEM1_MOUSE
AccessioniPrimary (citable) accession number: Q5F289
Secondary accession number(s): Q497J9, Q9D9D4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 23, 2007
Last sequence update: March 15, 2005
Last modified: November 2, 2016
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.