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Protein

DnaJ homolog subfamily C member 21

Gene

DNAJC21

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

May act as a co-chaperone for HSP70.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri314 – 33825C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri482 – 50625C2H2-type 2PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • poly(A) RNA binding Source: UniProtKB
  • zinc ion binding Source: InterPro

GO - Biological processi

  • protein folding Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
DnaJ homolog subfamily C member 21
Alternative name(s):
DnaJ homolog subfamily A member 5
Protein GS3
Gene namesi
Name:DNAJC21
Synonyms:DNAJA5
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

HGNCiHGNC:27030. DNAJC21.

Subcellular locationi

GO - Cellular componenti

  • ribosome Source: UniProtKB
Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA162383874.

Polymorphism and mutation databases

BioMutaiDNAJC21.
DMDMi296434479.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 531531DnaJ homolog subfamily C member 21PRO_0000281475Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei283 – 2831PhosphoserineCombined sources
Modified residuei302 – 3021PhosphoserineCombined sources
Modified residuei370 – 3701PhosphoserineCombined sources
Modified residuei511 – 5111PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ5F1R6.
MaxQBiQ5F1R6.
PeptideAtlasiQ5F1R6.
PRIDEiQ5F1R6.

PTM databases

iPTMnetiQ5F1R6.
PhosphoSiteiQ5F1R6.

Expressioni

Tissue specificityi

Brain, placenta, kidney and pancreas.

Gene expression databases

BgeeiQ5F1R6.
CleanExiHS_DNAJC21.
GenevisibleiQ5F1R6. HS.

Organism-specific databases

HPAiHPA040789.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
HTTP428586EBI-2654581,EBI-466029
MTERF1Q995512EBI-2654581,EBI-2690033

Protein-protein interaction databases

BioGridi126389. 12 interactions.
IntActiQ5F1R6. 8 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ5F1R6.
SMRiQ5F1R6. Positions 4-116.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 6967JPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi225 – 375151Glu-richAdd
BLAST
Compositional biasi384 – 3929Poly-Lys

Sequence similaritiesi

Contains 2 C2H2-type zinc fingers.PROSITE-ProRule annotation
Contains 1 J domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri314 – 33825C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri482 – 50625C2H2-type 2PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00510000047097.
HOGENOMiHOG000193307.
HOVERGENiHBG081458.
InParanoidiQ5F1R6.
KOiK09506.
OMAiQNYDDNF.
OrthoDBiEOG72VH6D.
PhylomeDBiQ5F1R6.
TreeFamiTF314518.

Family and domain databases

Gene3Di1.10.287.110. 1 hit.
InterProiIPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR022755. Znf_C2H2_jaz.
IPR003604. Znf_U1.
[Graphical view]
PfamiPF00226. DnaJ. 1 hit.
PF12171. zf-C2H2_jaz. 1 hit.
[Graphical view]
PRINTSiPR00625. JDOMAIN.
SMARTiSM00271. DnaJ. 1 hit.
SM00355. ZnF_C2H2. 2 hits.
SM00451. ZnF_U1. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
PROSITEiPS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 2 hits.
PS50157. ZINC_FINGER_C2H2_2. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q5F1R6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKCHYEALGV RRDASEEELK KAYRKLALKW HPDKNLDNAA EAAEQFKLIQ
60 70 80 90 100
AAYDVLSDPQ ERAWYDNHRE ALLKGGFDGE YQDDSLDLLR YFTVTCYSGY
110 120 130 140 150
GDDEKGFYTV YRNVFEMIAK EELESVLEEE VDDFPTFGDS QSDYDTVVHP
160 170 180 190 200
FYAYWQSFCT QKNFAWKEEY DTRQASNRWE KRAMEKENKK IRDKARKEKN
210 220 230 240 250
ELVRQLVAFI RKRDKRVQAH RKLVEEQNAE KARKAEEMRR QQKLKQAKLV
260 270 280 290 300
EQYREQSWMT MANLEKELQE MEARYEKEFG DGSDENEMEE HELKDEEDGK
310 320 330 340 350
DSDEAEDAEL YDDLYCPACD KSFKTEKAMK NHEKSKKHRE MVALLKQQLE
360 370 380 390 400
EEEENFSRPQ IDENPLDDNS EEEMEDAPKQ KLSKKQKKKK QKPAQNYDDN
410 420 430 440 450
FNVNGPGEGV KVDPEDTNLN QDSAKELEDS PQENVSVTEI IKPCDDPKSE
460 470 480 490 500
AKSVPKPKGK KTKDMKKPVR VPAEPQTMSV LISCTTCHSE FPSRNKLFDH
510 520 530
LKATGHARAP SSSSLNSATS SQSKKEKRKN R
Length:531
Mass (Da):62,028
Last modified:May 18, 2010 - v2
Checksum:iD1D3ED9AD6505613
GO
Isoform 2 (identifier: Q5F1R6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     395-395: Q → QDVPGKDSYLPAAHFQMAWGKKCVLGERRDGESEHKCAKMLLENRQ

Note: No experimental confirmation available.
Show »
Length:576
Mass (Da):67,141
Checksum:iAA936DB87C834334
GO
Isoform 3 (identifier: Q5F1R6-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     381-381: K → KVKYLTFRFIFALR

Note: No experimental confirmation available.
Show »
Length:544
Mass (Da):63,684
Checksum:i59B109CAB7597022
GO

Sequence cautioni

The sequence AAH65745.1 differs from that shown.Contaminating sequence. Potential poly-A sequence.Curated
The sequence AAI07578.1 differs from that shown.Contaminating sequence. Potential poly-A sequence.Curated
The sequence BAC87112.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti509 – 5091A → V in AAP81807 (PubMed:15067379).Curated
Sequence conflicti509 – 5091A → V in AAO46910 (Ref. 2) Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti214 – 2141D → N in a breast cancer sample; somatic mutation. 1 Publication
VAR_036163
Natural varianti433 – 4331E → K.
Corresponds to variant rs34908091 [ dbSNP | Ensembl ].
VAR_061145

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei381 – 3811K → KVKYLTFRFIFALR in isoform 3. 1 PublicationVSP_024004
Alternative sequencei395 – 3951Q → QDVPGKDSYLPAAHFQMAWG KKCVLGERRDGESEHKCAKM LLENRQ in isoform 2. 1 PublicationVSP_024005

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY258422 mRNA. Translation: AAP81807.1.
AY177688 mRNA. Translation: AAO46910.1.
BC050472 mRNA. Translation: AAH50472.1.
BC065745 mRNA. Translation: AAH65745.1. Sequence problems.
BC107577 mRNA. Translation: AAI07578.1. Sequence problems.
AK127749 mRNA. Translation: BAC87112.1. Different initiation.
CCDSiCCDS34144.1. [Q5F1R6-1]
CCDS3907.2. [Q5F1R6-2]
RefSeqiNP_001012339.2. NM_001012339.2. [Q5F1R6-1]
NP_919259.3. NM_194283.3. [Q5F1R6-2]
XP_005248306.1. XM_005248249.3. [Q5F1R6-3]
UniGeneiHs.131887.

Genome annotation databases

EnsembliENST00000342382; ENSP00000343728; ENSG00000168724. [Q5F1R6-1]
ENST00000382021; ENSP00000371451; ENSG00000168724. [Q5F1R6-2]
GeneIDi134218.
KEGGihsa:134218.
UCSCiuc003jjb.3. human. [Q5F1R6-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY258422 mRNA. Translation: AAP81807.1.
AY177688 mRNA. Translation: AAO46910.1.
BC050472 mRNA. Translation: AAH50472.1.
BC065745 mRNA. Translation: AAH65745.1. Sequence problems.
BC107577 mRNA. Translation: AAI07578.1. Sequence problems.
AK127749 mRNA. Translation: BAC87112.1. Different initiation.
CCDSiCCDS34144.1. [Q5F1R6-1]
CCDS3907.2. [Q5F1R6-2]
RefSeqiNP_001012339.2. NM_001012339.2. [Q5F1R6-1]
NP_919259.3. NM_194283.3. [Q5F1R6-2]
XP_005248306.1. XM_005248249.3. [Q5F1R6-3]
UniGeneiHs.131887.

3D structure databases

ProteinModelPortaliQ5F1R6.
SMRiQ5F1R6. Positions 4-116.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi126389. 12 interactions.
IntActiQ5F1R6. 8 interactions.

PTM databases

iPTMnetiQ5F1R6.
PhosphoSiteiQ5F1R6.

Polymorphism and mutation databases

BioMutaiDNAJC21.
DMDMi296434479.

Proteomic databases

EPDiQ5F1R6.
MaxQBiQ5F1R6.
PeptideAtlasiQ5F1R6.
PRIDEiQ5F1R6.

Protocols and materials databases

DNASUi134218.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000342382; ENSP00000343728; ENSG00000168724. [Q5F1R6-1]
ENST00000382021; ENSP00000371451; ENSG00000168724. [Q5F1R6-2]
GeneIDi134218.
KEGGihsa:134218.
UCSCiuc003jjb.3. human. [Q5F1R6-1]

Organism-specific databases

CTDi134218.
GeneCardsiDNAJC21.
H-InvDBHIX0004801.
HGNCiHGNC:27030. DNAJC21.
HPAiHPA040789.
neXtProtiNX_Q5F1R6.
PharmGKBiPA162383874.
GenAtlasiSearch...

Phylogenomic databases

GeneTreeiENSGT00510000047097.
HOGENOMiHOG000193307.
HOVERGENiHBG081458.
InParanoidiQ5F1R6.
KOiK09506.
OMAiQNYDDNF.
OrthoDBiEOG72VH6D.
PhylomeDBiQ5F1R6.
TreeFamiTF314518.

Miscellaneous databases

ChiTaRSiDNAJC21. human.
GenomeRNAii134218.
PROiQ5F1R6.

Gene expression databases

BgeeiQ5F1R6.
CleanExiHS_DNAJC21.
GenevisibleiQ5F1R6. HS.

Family and domain databases

Gene3Di1.10.287.110. 1 hit.
InterProiIPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR022755. Znf_C2H2_jaz.
IPR003604. Znf_U1.
[Graphical view]
PfamiPF00226. DnaJ. 1 hit.
PF12171. zf-C2H2_jaz. 1 hit.
[Graphical view]
PRINTSiPR00625. JDOMAIN.
SMARTiSM00271. DnaJ. 1 hit.
SM00355. ZnF_C2H2. 2 hits.
SM00451. ZnF_U1. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
PROSITEiPS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 2 hits.
PS50157. ZINC_FINGER_C2H2_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of a novel human cDNA encoding a J-domain protein (DNAJA5) from the fetal brain."
    Chen J., Yin G., Lu Y., Lou M., Cheng H., Ni X., Hu G., Luo C., Ying K., Xie Y., Mao Y.
    Int. J. Mol. Med. 13:735-740(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Fetal brain.
  2. "Cloning and characterization of a novel GS3 protein."
    Chen J., Xie Y., Mao Y.
    Submitted (NOV-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 126-458 (ISOFORM 3).
    Tissue: Skin and Uterus.
  4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 116-531 (ISOFORM 2).
    Tissue: Brain.
  5. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
    Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
    Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-283; SER-302 AND SER-370, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-370, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  7. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
    Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
    Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-370, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Leukemic T-cell.
  8. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-283 AND SER-370, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
    Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
    Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-283, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-511, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  12. Cited for: VARIANT [LARGE SCALE ANALYSIS] ASN-214.

Entry informationi

Entry nameiDJC21_HUMAN
AccessioniPrimary (citable) accession number: Q5F1R6
Secondary accession number(s): Q3B7J9
, Q6P086, Q6ZS43, Q86VC6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: May 18, 2010
Last modified: July 6, 2016
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.