Ribosome-inactivating protein gynostemmin precursor - Gynostemma pentaphyllum (Jiaogulan)

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Reviewed, UniProtKB/Swiss-Prot Q5F0I3 (RIP1_GYNPE)

Last modified June 16, 2009. Version 20. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein names	Recommended name: Ribosome-inactivating protein gynostemmin EC=3.2.2.22 Alternative name(s): rRNA N-glycosidase
Organism	Gynostemma pentaphyllum (Jiaogulan)
Taxonomic identifier	182084 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Cucurbitales › Cucurbitaceae › Gynostemma

Protein attributes

Sequence length	300 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Ribosome-inactivating protein of type 1, inhibits protein synthesis in animal cells By similarity.
Catalytic activity	Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA. UniProtKB P16094
Sequence similarities	Belongs to the ribosome-inactivating protein family. Type 1 RIP subfamily.

Ontologies

Keywords
Biological process	Plant defense
Domain	Signal
Molecular function	Hydrolase Protein synthesis inhibitor Toxin
PTM	Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	defense response Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of translation Inferred from electronic annotation. Source: UniProtKB-KW pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	rRNA N-glycosylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

23

Chain

277

Ribosome-inactivating protein gynostemmin Ref.2

PRO_0000271232

Sites

Active site

1

By similarity UniProtKB P16094

Amino acid modifications

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict

1

D → G in AAP40648. Ref.1

Sequence conflict

1

D → G in AAP40650. Ref.1

Sequence conflict

1

N → K in AAP33436. Ref.1

Sequence conflict

1

N → K in AAP33435. Ref.1

Sequence conflict

1

N → K in AAP40648. Ref.1

Sequence conflict

1

N → K in AAP40649. Ref.1

Sequence conflict

1

N → K in AAP40650. Ref.1

Sequence conflict

1

F → R in AAN78135. Ref.1

Sequence conflict

1

T → A in AAP40648. Ref.1

Sequence conflict

29

Missing in AAP40648. Ref.1

Sequence conflict

29

Missing in AAP40650. Ref.1

Sequence conflict

1

N → S in AAP40649. Ref.1

Sequence conflict

2

GD → NA in AAL82822. Ref.1

Sequence conflict

2

GD → NA in AAN08104. Ref.1

Sequence conflict

2

GD → NA in AAP33437. Ref.1

Sequence conflict

2

GD → NA in AAP40649. Ref.1

Sequence conflict

2

GD → NA in AAP40651. Ref.1

Sequence conflict

1

T → A in AAN78135. Ref.1

Sequence conflict

1

S → P in AAP40649. Ref.1

Sequence conflict

1

D → E in ABG76001. Ref.3

Sequence conflict

1

K → Q in AAL82822. Ref.1

Sequence conflict

1

K → Q in AAN08104. Ref.1

Sequence conflict

1

K → Q in AAP33437. Ref.1

Sequence conflict

1

K → Q in AAP40651. Ref.1

Sequence conflict

1

E → K in ABG76001. Ref.3

Sequence conflict

1

R → G in AAL82822. Ref.1

Sequence conflict

1

R → G in AAN08104. Ref.1

Sequence conflict

1

R → G in AAP33437. Ref.1

Sequence conflict

1

R → G in AAP40651. Ref.1

Sequence conflict

1

R → G in AAS15568. Ref.1

Sequence conflict

1

G → R in AAL82822. Ref.1

Sequence conflict

1

G → R in AAN08104. Ref.1

Sequence conflict

1

G → R in AAP33437. Ref.1

Sequence conflict

1

G → R in AAP40651. Ref.1

Sequence conflict

1

G → R in AAS15568. Ref.1

Sequence conflict

1

D → N in AAL82822. Ref.1

Sequence conflict

1

D → N in AAN08104. Ref.1

Sequence conflict

1

D → N in AAP33437. Ref.1

Sequence conflict

1

D → N in AAP40651. Ref.1

Sequence conflict

1

D → N in AAS15568. Ref.1

Sequence conflict

1

F → L in AAN78135. Ref.1

Sequence conflict

1

R → H in AAL82822. Ref.1

Sequence conflict

1

R → H in AAN08104. Ref.1

Sequence conflict

1

R → H in AAP33437. Ref.1

Sequence conflict

1

R → H in AAP40651. Ref.1

Sequence conflict

1

R → H in AAS15568. Ref.1

Sequence conflict

1

R → H in ABG76001. Ref.3

Sequence conflict

1

V → M in AAN78134. Ref.1

Sequence conflict

1

E → G in AAP40649. Ref.1

Sequence conflict

1

K → Q in AAL82822. Ref.1

Sequence conflict

1

K → Q in AAN08104. Ref.1

Sequence conflict

1

K → Q in AAP33437. Ref.1

Sequence conflict

1

K → Q in AAP40651. Ref.1

Sequence conflict

1

K → Q in AAS15568. Ref.1

Sequence conflict

2

Missing in AAL82822. Ref.1

Sequence conflict

2

Missing in AAN08104. Ref.1

Sequence conflict

2

Missing in AAP33437. Ref.1

Sequence conflict

2

Missing in AAP40649. Ref.1

Sequence conflict

2

Missing in AAP40651. Ref.1

Sequence conflict

2

Missing in AAS15568. Ref.1

Sequence conflict

1

P → T in AAN08103. Ref.1

Sequence conflict

1

Q → R in AAL82822. Ref.1

Sequence conflict

1

Q → R in AAN08104. Ref.1

Sequence conflict

1

Q → R in AAN78135. Ref.1

Sequence conflict

1

Q → R in AAP33437. Ref.1

Sequence conflict

1

Q → R in AAP40649. Ref.1

Sequence conflict

1

Q → R in AAP40651. Ref.1

Sequence conflict

1

Q → R in AAS15568. Ref.1

Sequence conflict

1

I → L in AAL82822. Ref.1

Sequence conflict

1

I → L in AAN08104. Ref.1

Sequence conflict

1

I → L in AAP33437. Ref.1

Sequence conflict

1

I → L in AAP40649. Ref.1

Sequence conflict

1

I → L in AAP40651. Ref.1

Sequence conflict

1

I → L in AAS15568. Ref.1

Sequence conflict

2

QK → LF in AAL82822. Ref.1

Sequence conflict

1

D → G in AAL82822. Ref.1

Sequence conflict

1

D → G in AAN08104. Ref.1

Sequence conflict

1

D → G in AAP40649. Ref.1

Sequence conflict

1

D → G in AAP40651. Ref.1

Sequence conflict

1

D → G in AAS15568. Ref.1

Sequence conflict

1

L → P in AAL82822. Ref.1

Sequence conflict

1

L → P in AAN08104. Ref.1

Sequence conflict

1

L → P in AAP40649. Ref.1

Sequence conflict

1

L → P in AAP40651. Ref.1

Sequence conflict

1

L → P in AAS15568. Ref.1

Sequence conflict

1

L → P in ABG76001. Ref.3

Sequence conflict

4

SGKP → KNGS in AAL82822. Ref.1

Sequence conflict

4

SGKP → KNGS in AAN08104. Ref.1

Sequence conflict

4

SGKP → KNGS in AAP40649. Ref.1

Sequence conflict

4

SGKP → KNGS in AAP40651. Ref.1

Sequence conflict

4

SGKP → KNGS in AAS15568. Ref.1

Sequence conflict

1

Y → S in AAL82822. Ref.1

Sequence conflict

1

Y → S in AAN08104. Ref.1

Sequence conflict

1

Y → S in AAP40649. Ref.1

Sequence conflict

1

Y → S in AAP40651. Ref.1

Sequence conflict

1

Y → S in AAS15568. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

Q5F0I3-1 [UniParc].

Last modified January 9, 2007. Version 2.
Checksum: A4860B0168BDD438
Show »

300

33,505

        10         20         30         40         50         60 
MRVARFCTVV AILLYFGFHI AECDINFSLA GADGQTYNTF IAKLRQELSI GTQKVANITV 

        70         80         90        100        110        120 
LKHHVSSNTQ RFLSINLFNY NGDKITLGID VFDVYVVGFL TGTNSYIFKE APDLAYNKSL 

       130        140        150        160        170        180 
LFPGSVRENL SYTGGYDDLE RRGAGREDIP LGLLPLDTAI TNLFRRDSTS FRRSFIVIIQ 

       190        200        210        220        230        240 
MVSEAARFKI IEAKIAKNLY GENTFKPDQA IISLENNWGA LSKQIQKAQD RGGVFPNLVT 

       250        260        270        280        290        300 
LTTSSGKPLI IRNDSDPLVQ NGIALLKYMS ERSVEYSITD DTVNESNGAF LEIMEIHDLM

References

[1]	"Gynostemmin, full-length sequence with one ORF encoding ribosome-inactivating protein from Gynostemma pentaphyllum (Thunb.) Makino." Lin Y., Wu Z.J., Xie L.H., Lin Q.Y. Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
[2]	Lin Y., Wu Z.J., Lin Q.Y., Xie L.H. Submitted (DEC-2001) to UniProtKB Cited for: PROTEIN SEQUENCE OF 24-42. Tissue: Leaf and Stem.
[3]	"A gene encoding ribosome-inactivating protein from Gynostemma pentaphyllum." Xiang T., Xu J. Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 31-300.

Cross-references

Sequence databases
	AY075115 Genomic DNA. Translation: AAL82822.2. AY134616 mRNA. Translation: AAN08103.1. AY134617 mRNA. Translation: AAN08104.1. AY160767 mRNA. Translation: AAN78134.1. AY160768 mRNA. Translation: AAN78135.1. AY160769 mRNA. Translation: AAN78136.1. AY279104 mRNA. Translation: AAP40648.1. AY279105 mRNA. Translation: AAP40649.1. AY279106 mRNA. Translation: AAP40650.1. AY279107 mRNA. Translation: AAP40651.1. AY279218 mRNA. Translation: AAP33435.1. AY279219 mRNA. Translation: AAP33436.1. AY279220 mRNA. Translation: AAP33437.1. AY513740 mRNA. Translation: AAS15568.1. DQ672577 Genomic DNA. Translation: ABG76001.1.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.2.2.22. 291311.
Family and domain databases
InterPro	IPR001574. Ribosome_inactivat_prot. IPR017988. Ribosome_inactivat_prot_CS. IPR016138. Ribosome_inactivat_prot_sub1. IPR017989. Ribosome_inactivat_prot_subgr. [Graphical view]
Gene3D	G3DSA:3.40.420.10. Ribosome_inactivat_prot_sub1. 1 hit.
Pfam	PF00161. RIP. 1 hit. [Graphical view]
PRINTS	PR00396. SHIGARICIN.
PROSITE	PS00275. SHIGA_RICIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIP1_GYNPE

Accession

Primary (citable) accession number: Q5F0I3
Secondary accession number(s): P83206

expand/collapse secondary AC list

Q8RY69

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 9, 2007
Last sequence update:	January 9, 2007
Last modified:	June 16, 2009
This is version 20 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents