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Protein

Ectonucleotide pyrophosphatase/phosphodiesterase family member 7

Gene

Enpp7

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Converts sphingomyelin to ceramide. Also has phospholipase C activity toward palmitoyl lyso-phosphocholine. Does not appear to have nucleotide pyrophosphatase activity.

Catalytic activityi

Sphingomyelin + H2O = N-acylsphingosine + phosphocholine.

pH dependencei

Optimum pH is 9.0.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei74 – 741NucleophileBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Ectonucleotide pyrophosphatase/phosphodiesterase family member 7 (EC:3.1.4.12)
Short name:
E-NPP 7
Short name:
NPP-7
Alternative name(s):
Alkaline sphingomyelin phosphodiesterase
Intestinal alkaline sphingomyelinase
Short name:
Alk-SMase
Gene namesi
Name:Enpp7
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi1359324. Enpp7.

Subcellular locationi

  • Membrane Curated; Single-pass type I membrane protein Curated

  • Note: Localized at the surface of the microvillar membrane in small intestine enterocytes, and in endosome-like structures situated beneath the microvillar membrane, and in Golgi complex.By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei423 – 43917HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Sequence analysisAdd
BLAST
Chaini22 – 439418Ectonucleotide pyrophosphatase/phosphodiesterase family member 7PRO_0000036404Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi99 – 991N-linked (GlcNAc...)Sequence analysis
Glycosylationi120 – 1201N-linked (GlcNAc...)Sequence analysis
Glycosylationi145 – 1451N-linked (GlcNAc...)Sequence analysis
Glycosylationi167 – 1671N-linked (GlcNAc...)Sequence analysis
Glycosylationi266 – 2661N-linked (GlcNAc...)Sequence analysis

Post-translational modificationi

N-glycosylated; required for activity and transport to the plasma membrane.By similarity

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ5EZ72.
PRIDEiQ5EZ72.

Expressioni

Tissue specificityi

Highly expressed in the jejunum.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000064040.

Structurei

3D structure databases

ProteinModelPortaliQ5EZ72.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni72 – 787Required for enzyme activityBy similarity

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2645. Eukaryota.
COG1524. LUCA.
HOVERGENiHBG106748.
InParanoidiQ5EZ72.
KOiK12354.
PhylomeDBiQ5EZ72.

Family and domain databases

Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR017850. Alkaline_phosphatase_core.
IPR024873. E-NPP.
IPR029895. ENPP7.
IPR002591. Phosphodiest/P_Trfase.
[Graphical view]
PANTHERiPTHR10151. PTHR10151. 1 hit.
PTHR10151:SF63. PTHR10151:SF63. 1 hit.
PfamiPF01663. Phosphodiest. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5EZ72-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGHSAVLLSV ALVILPACVT GGPVQRQQQH KLLLVSFDGF RWNYDQDVET
60 70 80 90 100
PNLDSMAQEG VKARYMTPAF VTMTSPCHFT LVTGKYIENH GVVHNMFYNT
110 120 130 140 150
TNKVRLPYHA TLGIQRWWDN GSIPIWITAQ RQGLKTGSFF YPGGNVTYQG
160 170 180 190 200
EAVTMSRKEG VLHNYKNETE WRANVDTVMK WFTEEDVSLV TLYFGEPDST
210 220 230 240 250
GHKYGPESQE RKDMVKQVDR TVGYLRDSIK RHHLTDSLNL IITSDHGMTT
260 270 280 290 300
VNKKASDLVE FHKFPNFTFR DIEFELLDYG PNGMLIPKEG MLEKVYSVLK
310 320 330 340 350
DAHPRLHVYK KEDFPKTFHY ANNPRITSLL MYSDLGYVIH GRVNVQFNSG
360 370 380 390 400
EHGFDNQDMD MKTIFRAVGP SFKAGLEVEP FESVHVYELM CQLLGIVPEP
410 420 430
NDGHPGVLQP MLRSGSPLSR QHHLVVVLMG ILTGLAKVV
Length:439
Mass (Da):49,837
Last modified:March 15, 2005 - v1
Checksum:i7CEABF0393C9480F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY568760 mRNA. Translation: AAU03450.1.
RefSeqiNP_001012484.1. NM_001012466.1.
UniGeneiRn.211790.

Genome annotation databases

GeneIDi303729.
KEGGirno:303729.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY568760 mRNA. Translation: AAU03450.1.
RefSeqiNP_001012484.1. NM_001012466.1.
UniGeneiRn.211790.

3D structure databases

ProteinModelPortaliQ5EZ72.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000064040.

Proteomic databases

PaxDbiQ5EZ72.
PRIDEiQ5EZ72.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi303729.
KEGGirno:303729.

Organism-specific databases

CTDi339221.
RGDi1359324. Enpp7.

Phylogenomic databases

eggNOGiKOG2645. Eukaryota.
COG1524. LUCA.
HOVERGENiHBG106748.
InParanoidiQ5EZ72.
KOiK12354.
PhylomeDBiQ5EZ72.

Miscellaneous databases

PROiQ5EZ72.

Family and domain databases

Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR017850. Alkaline_phosphatase_core.
IPR024873. E-NPP.
IPR029895. ENPP7.
IPR002591. Phosphodiest/P_Trfase.
[Graphical view]
PANTHERiPTHR10151. PTHR10151. 1 hit.
PTHR10151:SF63. PTHR10151:SF63. 1 hit.
PfamiPF01663. Phosphodiest. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Cloning of alkaline sphingomyelinase from rat intestinal mucosa and adjusting of the hypothetical protein XP_221184 in GenBank."
    Wu J., Cheng Y., Palmberg C., Bergman T., Nilsson A., Duan R.-D.
    Biochim. Biophys. Acta 1687:94-102(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 32-40; 106-116; 204-211; 221-226; 255-263 AND 317-342, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
    Strain: Sprague-Dawley.
    Tissue: Intestinal mucosa.

Entry informationi

Entry nameiENPP7_RAT
AccessioniPrimary (citable) accession number: Q5EZ72
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: March 15, 2005
Last modified: June 8, 2016
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.