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Reviewed, UniProtKB/Swiss-Prot Q5EZ72 (ENPP7_RAT)

Last modified October 13, 2009. Version 35. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Ectonucleotide pyrophosphatase/phosphodiesterase family member 7
      Short name=E-NPP7
      Short name=NPP-7
    EC=3.1.4.12
Alternative name(s):
    Alkaline sphingomyelin phosphodiesterase
    Intestinal alkaline sphingomyelinase
      Short name=Alk-SMase
Gene names
Name: Enpp7
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

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Protein attributes

Sequence length439 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Converts sphingomyelin to ceramide. Also has phospholipase C activity toward palmitoyl lyso-phosphocholine. Does not appear to have nucleotide pyrophosphatase activity.

Catalytic activity

Sphingomyelin + H2O = N-acylsphingosine + choline phosphate.

Subcellular location

Membrane; Single-pass type I membrane protein Potential. Note: Localized at the surface of the microvillar membrane in small intestine enterocytes, and in endosome-like structures situated beneath the microvillar membrane, and in Golgi complex By similarity.

Tissue specificity

Highly expressed in the jejunum. Ref.1

Post-translational modification

N-glycosylated; required for activity and transport to the plasma membrane By similarity.

Sequence similarities

Belongs to the nucleotide pyrophosphatase/phosphodiesterase family.

Biophysicochemical properties

pH dependence:

Optimum pH is 9.0.

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Ontologies

Keywords
   Cellular componentMembrane
   DomainSignal
Transmembrane
   Molecular functionHydrolase
   PTMGlycoprotein
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological processmetabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular componentintegral to membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionsphingomyelin phosphodiesterase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 Potential
Chain22 – 439418Ectonucleotide pyrophosphatase/phosphodiesterase family member 7
PRO_0000036404

Regions

Transmembrane423 – 43917 Potential
Region72 – 787Required for enzyme activity By similarity

Sites

Active site741 By similarity

Amino acid modifications

Glycosylation991N-linked (GlcNAc...) Potential
Glycosylation1201N-linked (GlcNAc...) Potential
Glycosylation1451N-linked (GlcNAc...) Potential
Glycosylation1671N-linked (GlcNAc...) Potential
Glycosylation2661N-linked (GlcNAc...) Potential

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Sequences

Sequence LengthMass (Da)Tools
Q5EZ72-1 [UniParc].

Last modified March 15, 2005. Version 1.
Checksum: 7CEABF0393C9480F

FASTA43949,837
        10         20         30         40         50         60 
MGHSAVLLSV ALVILPACVT GGPVQRQQQH KLLLVSFDGF RWNYDQDVET PNLDSMAQEG 

        70         80         90        100        110        120 
VKARYMTPAF VTMTSPCHFT LVTGKYIENH GVVHNMFYNT TNKVRLPYHA TLGIQRWWDN 

       130        140        150        160        170        180 
GSIPIWITAQ RQGLKTGSFF YPGGNVTYQG EAVTMSRKEG VLHNYKNETE WRANVDTVMK 

       190        200        210        220        230        240 
WFTEEDVSLV TLYFGEPDST GHKYGPESQE RKDMVKQVDR TVGYLRDSIK RHHLTDSLNL 

       250        260        270        280        290        300 
IITSDHGMTT VNKKASDLVE FHKFPNFTFR DIEFELLDYG PNGMLIPKEG MLEKVYSVLK 

       310        320        330        340        350        360 
DAHPRLHVYK KEDFPKTFHY ANNPRITSLL MYSDLGYVIH GRVNVQFNSG EHGFDNQDMD 

       370        380        390        400        410        420 
MKTIFRAVGP SFKAGLEVEP FESVHVYELM CQLLGIVPEP NDGHPGVLQP MLRSGSPLSR 

       430 
QHHLVVVLMG ILTGLAKVV

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References

[1]"Cloning of alkaline sphingomyelinase from rat intestinal mucosa and adjusting of the hypothetical protein XP_221184 in GenBank."
Wu J., Cheng Y., Palmberg C., Bergman T., Nilsson A., Duan R.-D.
Biochim. Biophys. Acta 1687:94-102(2005) [PubMed: 15708357] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 32-40; 106-116; 204-211; 221-226; 255-263 AND 317-342, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
Strain: Sprague-Dawley.
Tissue: Intestinal mucosa.
+Additional computationally mapped references.

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Cross-references

Sequence databases

AY568760 mRNA. Translation: AAU03450.1.
IPIIPI00553948.
RefSeqNP_001012484.1.
UniGeneRn.211790

3D structure databases

ModBaseSearch...

Proteomic databases

PRIDEQ5EZ72.

Genome annotation databases

GeneID303729.
KEGGrno:303729.

Organism-specific databases

CTD303729.
RGD1359324. Enpp7.

Phylogenomic databases

HOVERGENQ5EZ72.

Enzyme and pathway databases

BRENDA3.1.4.12. 248.

Gene expression databases

GenevestigatorQ5EZ72.

Family and domain databases

InterProIPR017849. Alkaline_Pase-like_a/b/a.
IPR002591. Phosphodiest/P_Trfase.
[Graphical view]
Gene3DG3DSA:3.40.720.10. Alk_phosphtse. 1 hit.
PfamPF01663. Phosphodiest. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio651948.

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Entry information

Entry nameENPP7_RAT
AccessionPrimary (citable) accession number: Q5EZ72
Entry history
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: March 15, 2005
Last modified: October 13, 2009
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents