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Q5EP31 (Q5EP31_9INFA) Unreviewed, UniProtKB/TrEMBL

Last modified June 11, 2014. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein names

Hemagglutinin HA EMBL AAW80717.1
Gene names
Name:HA EMBL ABP51977.1
OrganismInfluenza A virus (A/Viet Nam/1203/2004(H5N1)) EMBL AAW80717.1
Taxonomic identifier284218 [NCBI]
Taxonomic lineageVirusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus A

Protein attributes

Sequence length568 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization of about two third of the virus particles through clathrin-dependent endocytosis and about one third through a clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore By similarity. SAAS SAAS008980 RuleBase RU003324

Subunit structure

Homotrimer of disulfide-linked HA1-HA2 By similarity. SAAS SAAS008980 RuleBase RU003324

Subcellular location

Virion membrane; Single-pass type I membrane protein. Host apical cell membrane; Single-pass type I membrane protein By similarity SAAS SAAS008980.

Sequence similarities

Belongs to the influenza viruses hemagglutinin family. RuleBase RU003324

Ontologies

Keywords
   Biological processClathrin- and caveolin-independent endocytosis of virus by host SAAS SAAS008980
Clathrin-mediated endocytosis of virus by host SAAS SAAS008980
Fusion of virus membrane with host endosomal membrane SAAS SAAS008980
Fusion of virus membrane with host membrane
Host-virus interaction
Viral attachment to host cell SAAS SAAS008980
Viral penetration into host cytoplasm
Virus endocytosis by host
Virus entry into host cell
   Cellular componentHost cell membrane SAAS SAAS008980
Host membrane
Membrane
Viral envelope protein SAAS SAAS008980 RuleBase RU003324
Virion
   DomainTransmembrane
Transmembrane helix SAAS SAAS008980
   Molecular functionHemagglutinin SAAS SAAS008980 RuleBase RU003324
   PTMDisulfide bond SAAS SAAS008980
   Technical term3D-structure PDB 4BH2 PDB 4N5Z PDB 4BH3 PDB 4BH4
Gene Ontology (GO)
   Biological_processclathrin-mediated endocytosis of virus by host cell

Inferred from electronic annotation. Source: UniProtKB-KW

fusion of virus membrane with host endosome membrane

Inferred from electronic annotation. Source: UniProtKB-KW

fusion of virus membrane with host plasma membrane

Inferred from electronic annotation. Source: InterPro

virion attachment to host cell

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componenthost cell plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

viral envelope

Inferred from electronic annotation. Source: UniProtKB-KW

virion membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Binding site2371Galactose; via carbonyl oxygen PDB 4BH4

Amino acid modifications

Glycosylation271N-linked (GlcNAc...)
Glycosylation391N-linked (GlcNAc...) PDB 4N5Z
Glycosylation1811N-linked (GlcNAc...) PDB 4N5Z

Sequences

Sequence LengthMass (Da)Tools
Q5EP31 [UniParc].

Last modified March 15, 2005. Version 1.
Checksum: 1987CB02AB9C06CA

FASTA56864,461
        10         20         30         40         50         60 
MEKIVLLFAI VSLVKSDQIC IGYHANNSTE QVDTIMEKNV TVTHAQDILE KKHNGKLCDL 

        70         80         90        100        110        120 
DGVKPLILRD CSVAGWLLGN PMCDEFINVP EWSYIVEKAN PVNDLCYPGD FNDYEELKHL 

       130        140        150        160        170        180 
LSRINHFEKI QIIPKSSWSS HEASLGVSSA CPYQGKSSFF RNVVWLIKKN STYPTIKRSY 

       190        200        210        220        230        240 
NNTNQEDLLV LWGIHHPNDA AEQTKLYQNP TTYISVGTST LNQRLVPRIA TRSKVNGQSG 

       250        260        270        280        290        300 
RMEFFWTILK PNDAINFESN GNFIAPEYAY KIVKKGDSTI MKSELEYGNC NTKCQTPMGA 

       310        320        330        340        350        360 
INSSMPFHNI HPLTIGECPK YVKSNRLVLA TGLRNSPQRE RRRKKRGLFG AIAGFIEGGW 

       370        380        390        400        410        420 
QGMVDGWYGY HHSNEQGSGY AADKESTQKA IDGVTNKVNS IIDKMNTQFE AVGREFNNLE 

       430        440        450        460        470        480 
RRIENLNKKM EDGFLDVWTY NAELLVLMEN ERTLDFHDSN VKNLYDKVRL QLRDNAKELG 

       490        500        510        520        530        540 
NGCFEFYHKC DNECMESVRN GTYDYPQYSE EARLKREEIS GVKLESIGIY QILSIYSTVA 

       550        560 
SSLALAIMVA GLSLWMCSNG SLQCRICI 

« Hide

References

[1]"Evolution of H5N1 avian influenza viruses in Asia."
World Health Organization Global Influenza Program Surveillance Network
Emerg. Infect. Dis. 11:1515-1521(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Strain: A/Viet Nam/1203/2004 EMBL ABP51977.1.
[2]"Lethality to ferrets of H5N1 influenza viruses isolated from humans and poultry in 2004."
Govorkova E.A., Rehg J.E., Krauss S., Yen H.L., Guan Y., Peiris M., Nguyen T.D., Hanh T.H., Puthavathana P., Long H.T., Buranathai C., Lim W., Webster R.G., Hoffmann E.
J. Virol. 79:2191-2198(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Strain: A/Viet Nam/1203/2004 EMBL AAW80717.1.
[3]Smith C.
Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
Strain: A/Viet Nam/1203/2004 EMBL ABP51977.1.
[4]"Acquisition of virulence mutations in avian H5N1 influenza virus during a single passage in ferrets."
Butler J., Middleton D., Klippel J., Rockman S., Brown L., Sapats S.
Submitted (MAR-2010) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
Strain: A/Viet Nam/1203/2004 EMBL ADD97095.1.
[5]"Receptor binding by a ferret-transmissible H5 avian influenza virus."
Xiong X., Coombs P.J., Martin S.R., Liu J., Xiao H., McCauley J.W., Locher K., Walker P.A., Collins P.J., Kawaoka Y., Skehel J.J., Gamblin S.J.
Nature 497:392-396(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS) OF 17-340 AND 347-513 IN COMPLEX WITH GALACTOSE, GLYCOSYLATION AT ASN-27; ASN-39 AND ASN-181.
[6]"Hemagglutinin receptor specificity and structural analyses of respiratory droplet-transmissible H5N1 viruses."
de Vries R.P., Zhu X., McBride R., Rigter A., Hanson A., Zhong G., Hatta M., Xu R., Yu W., Kawaoka Y., de Haan C.A., Wilson I.A., Paulson J.C.
J. Virol. 88:768-773(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.95 ANGSTROMS) OF 17-346, GLYCOSYLATION AT ASN-39 AND ASN-181.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY818135 Genomic RNA. Translation: AAW80717.1.
EF541403 Viral cRNA. Translation: ABP51977.1.
HM006759 Viral cRNA. Translation: ADD97095.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4BH2X-ray2.12A17-340[»]
B347-513[»]
4BH3X-ray2.00A17-340[»]
B347-513[»]
4BH4X-ray1.90A17-340[»]
B347-513[»]
4N5ZX-ray2.95A/C/E/G/I/K/M/O/Q/S/U/W/Y/a/c17-346[»]
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-60227N.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D2.10.77.10. 1 hit.
3.90.20.10. 1 hit.
3.90.209.20. 1 hit.
InterProIPR008980. Capsid_hemagglutn.
IPR013828. Hemagglutn_HA1_a/b_dom.
IPR013827. Hemagglutn_HA1_b-rbn_dom.
IPR000149. Hemagglutn_influenz_A.
IPR001364. Hemagglutn_influenz_A/B.
IPR013829. Hemagglutn_stalk.
[Graphical view]
PfamPF00509. Hemagglutinin. 1 hit.
[Graphical view]
PRINTSPR00330. HEMAGGLUTN1.
PR00329. HEMAGGLUTN12.
SUPFAMSSF49818. SSF49818. 1 hit.
ProtoNetSearch...

Entry information

Entry nameQ5EP31_9INFA
AccessionPrimary (citable) accession number: Q5EP31
Entry history
Integrated into UniProtKB/TrEMBL: March 15, 2005
Last sequence update: March 15, 2005
Last modified: June 11, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)