Reviewed, UniProtKB/Swiss-Prot Q5EN22 (ERG6_MAGGR)

Names and origin

Protein names

Recommended name:
    Sterol 24-C-methyltransferase
    EC=2.1.1.41
Alternative name(s):
    Delta(24)-sterol C-methyltransferase

Gene names

Name:	ERG6
ORF Names:	MGG_04346

Organism

Magnaporthe grisea (Rice blast fungus) (Pyricularia grisea) [Complete proteome]

Taxonomic identifier

148305 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Sordariomycetidae › Magnaporthales › Magnaporthaceae › Magnaporthe

Protein attributes

Sequence length	390 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the methyl transfer from S-adenosyl-methionine to the C-24 of zymosterol to form fecosterol By similarity.
Catalytic activity	S-adenosyl-L-methionine + 5-alpha-cholesta-8,24-dien-3-beta-ol = S-adenosyl-L-homocysteine + 24-methylene-5-alpha-cholest-8-en-3-beta-ol.
Pathway	Steroid metabolism; ergosterol biosynthesis; ergosterol from zymosterol: step 1/5.
Sequence similarities	Belongs to the methyltransferase superfamily. ERG6 family.

Ontologies

Keywords
Biological process	Lipid synthesis Steroid biosynthesis Sterol biosynthesis
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	sterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	sterol 24-C-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 390	390	Sterol 24-C-methyltransferase		PRO_0000124794
Experimental info
	Sequence conflict	246	1	D → N in AAX07631. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q5EN22-1 [UniParc]. Last modified June 12, 2007. Version 2. Checksum: 4442B9609B8DA9D5	FASTA	390	43,036
	10 20 30 40 50 60 MSKQQHPSSK TQITRRRDTA DMTFEQVLHK GSSANMGGLS SMLGKDREAS KTAVDQYFRH 70 80 90 100 110 120 WDGKTAKTET TKVREERKAD YATLTRQYYN LVTDFYEYGW GQSFHFCTFA PGETFASAIT 130 140 150 160 170 180 RYEHTLAHRI GIKKGMKVLD IGCGVGGPAR QIAKFTGANI TGITINEYQV ERARRYAEME 190 200 210 220 230 240 GYGAGEQLKF VQGDFMALPF EKETFDAVYS IEATVHAPKL EDVYKQIFNV LKPGGIFGLY 250 260 270 280 290 300 EWVMTDAYDE NDPHHKEIRF GIEHGGGIAN LQTAQTAIAA IKAAGFELLE SEDLADNSDR 310 320 330 340 350 360 APWYWPLGGN AWQYASTFGD ILSTFTMTPA GRAIAHTVLG VVESIGLVPP GTKKTADSLS 370 380 390 TTAAALVAGG KEGLFTPMFL MVARKPVAKE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Dong H.T., Peng Y.L., Chen B.S., Li Y.Z., Li D.B. Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Y34. Tissue: Conidium.
[2]	"The genome sequence of the rice blast fungus Magnaporthe grisea." Dean R.A., Talbot N.J., Ebbole D.J., Farman M.L., Mitchell T.K., Orbach M.J., Thon M.R., Kulkarni R., Xu J.-R., Pan H., Read N.D., Lee Y.-H., Carbone I., Brown D., Oh Y.Y., Donofrio N., Jeong J.S., Soanes D.M. , Djonovic S., Kolomiets E., Rehmeyer C., Li W., Harding M., Kim S., Lebrun M.-H., Bohnert H., Coughlan S., Butler J., Calvo S.E., Ma L.-J., Nicol R., Purcell S., Nusbaum C., Galagan J.E., Birren B.W. Nature 434:980-986(2005) [PubMed: 15846337] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 70-15 / FGSC 8958.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY849610 mRNA. Translation: AAX07631.1. CH476831 Genomic DNA. Translation: EDJ99656.1.
RefSeq	XP_361872.1.
UniGene	Mgr.7893
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2677539.
KEGG	mgr:MGG_04346.
NMPDR	fig|242507.1.peg.3612.
Phylogenomic databases
OMA	Q5EN22. IMRILPA.
Enzyme and pathway databases
BRENDA	2.1.1.41. 5953.
Family and domain databases
InterPro	IPR013216. Methyltransf_11. IPR013705. Sterol_MeTrfase_C. [Graphical view]
Pfam	PF08241. Methyltransf_11. 1 hit. PF08498. Sterol_MT_C. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ERG6_MAGGR

Accession

Primary (citable) accession number: Q5EN22
Secondary accession number(s): A4R5U1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	June 12, 2007
Last modified:	May 26, 2009
This is version 27 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)