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Q5EK40

- CHXA_VIBCL

UniProt

Q5EK40 - CHXA_VIBCL

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Protein

Cholix toxin

Gene

chxA

Organism
Vibrio cholerae
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

An NAD-dependent ADP-ribosyltransferase (ADPRT), it catalyzes the transfer of the ADP-ribosyl moiety of oxidized NAD onto eukaryotic elongation factor 2 (eEF-2) thus arresting protein synthesis. It probably uses the eukaryotic prolow-density lipoprotein receptor-related protein 1 (LRP1) to enter mouse cells, although there seems to be at least one other receptor as well. Is active against mouse fibroblasts, Chinese hamster ovary eEF-2, brine shrimp (Artemia spp. nauplii) and upon expression in S.cerevisiae.2 Publications

Catalytic activityi

NAD+ + diphthamide-[translation elongation factor 2] = nicotinamide + N-(ADP-D-ribosyl)diphthamide-[translation elongation factor 2].

Enzyme regulationi

Partially inhibited by 1,8-naphthalimide (NAP).1 Publication

Kineticsi

For ADP-ribosyltransferase activity of a catalytic fragment of residues 459-666.

  1. KM=45 µM for NAD1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei613 – 6131Curated

GO - Molecular functioni

  1. NAD+-diphthamide ADP-ribosyltransferase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Toxin, Transferase

Keywords - Ligandi

NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Cholix toxin (EC:2.4.2.36)
Alternative name(s):
Exotoxin A
NAD(+)--diphthamide ADP-ribosyltransferase
Gene namesi
Name:chxA
Synonyms:toxA
OrganismiVibrio cholerae
Taxonomic identifieri666 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi606 – 6061E → A: Loss of ADPRT activity, loss of toxicity against yeast. 1 Publication
Mutagenesisi613 – 6131E → A: Loss of ADPRT activity, loss of toxicity against mouse cells, brine shrimp and yeast. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3232Sequence AnalysisAdd
BLAST
Chaini33 – 666634Cholix toxinPRO_0000409819Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi43 ↔ 47
Disulfide bondi240 ↔ 257
Disulfide bondi310 ↔ 332
Disulfide bondi426 ↔ 433

Post-translational modificationi

Probably requires further proteolytic processing to generate a fragment with toxic activity.

Keywords - PTMi

Disulfide bond

Structurei

Secondary structure

1
666
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi39 – 424Combined sources
Beta strandi47 – 493Combined sources
Beta strandi57 – 626Combined sources
Beta strandi71 – 8111Combined sources
Beta strandi94 – 996Combined sources
Turni100 – 1023Combined sources
Beta strandi103 – 1086Combined sources
Beta strandi113 – 1153Combined sources
Beta strandi120 – 1267Combined sources
Beta strandi131 – 1377Combined sources
Beta strandi139 – 15214Combined sources
Beta strandi157 – 16610Combined sources
Beta strandi170 – 1723Combined sources
Beta strandi177 – 1826Combined sources
Helixi185 – 1906Combined sources
Turni191 – 1933Combined sources
Beta strandi194 – 20310Combined sources
Beta strandi208 – 21912Combined sources
Helixi226 – 2305Combined sources
Helixi231 – 2344Combined sources
Helixi236 – 2416Combined sources
Helixi245 – 2484Combined sources
Beta strandi269 – 2735Combined sources
Beta strandi289 – 2935Combined sources
Helixi300 – 31011Combined sources
Helixi314 – 3174Combined sources
Helixi328 – 34619Combined sources
Helixi350 – 3545Combined sources
Helixi355 – 3584Combined sources
Helixi361 – 3633Combined sources
Helixi366 – 38116Combined sources
Helixi385 – 40319Combined sources
Helixi409 – 41810Combined sources
Beta strandi420 – 4256Combined sources
Beta strandi441 – 4455Combined sources
Helixi450 – 4534Combined sources
Beta strandi460 – 4623Combined sources
Beta strandi465 – 4684Combined sources
Helixi471 – 48313Combined sources
Beta strandi486 – 4949Combined sources
Helixi496 – 5049Combined sources
Helixi516 – 5205Combined sources
Beta strandi523 – 5297Combined sources
Helixi530 – 5345Combined sources
Helixi545 – 5473Combined sources
Helixi551 – 5555Combined sources
Beta strandi559 – 5668Combined sources
Helixi567 – 5726Combined sources
Beta strandi573 – 5753Combined sources
Helixi580 – 5823Combined sources
Helixi584 – 5918Combined sources
Beta strandi601 – 6077Combined sources
Beta strandi612 – 6165Combined sources
Helixi618 – 6214Combined sources
Beta strandi625 – 6328Combined sources
Helixi643 – 6497Combined sources
Helixi650 – 6523Combined sources
Helixi662 – 6643Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2Q5TX-ray2.10A33-666[»]
2Q6MX-ray1.25A459-666[»]
3ESSX-ray1.19A459-665[»]
3KI0X-ray1.29A459-665[»]
3KI1X-ray1.43A459-665[»]
3KI2X-ray1.28A459-665[»]
3KI3X-ray1.27A459-665[»]
3KI4X-ray1.65A459-665[»]
3KI5X-ray1.55A459-665[»]
3KI6X-ray1.54A459-665[»]
3KI7X-ray1.32A459-665[»]
3NY6X-ray1.68A459-665[»]
3Q9OX-ray1.79A33-666[»]
ProteinModelPortaliQ5EK40.
SMRiQ5EK40. Positions 37-662.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ5EK40.

Family & Domainsi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
3.90.1350.10. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR015185. Exotox-A_bind.
IPR015099. Exotox-A_cataly_dom.
IPR015186. Exotox-A_middle_dom.
[Graphical view]
PfamiPF09101. Exotox-A_bind. 1 hit.
PF09009. Exotox-A_cataly. 1 hit.
PF09102. Exotox-A_target. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 1 hit.
SSF56864. SSF56864. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5EK40-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MYLTFYLEKV MKKMLLIAGA TVISSMAHPT FAVEDELNIF DECRSPCSLT
60 70 80 90 100
PEPGKPIQSK LSIPSDVVLD EGVLYYSMTI NDEQNDIKDE DKGESIITIG
110 120 130 140 150
EFATVRATRH YVNQDAPFGV IHLDITTENG TKTYSYNRKE GEFAINWLVP
160 170 180 190 200
IGEDSPASIK ISVDELDQQR NIIEVPKLYS IDLDNQTLEQ WKTQGNVSFS
210 220 230 240 250
VTRPEHNIAI SWPSVSYKAA QKEGSRHKRW AHWHTGLALC WLVPMDAIYN
260 270 280 290 300
YITQQNCTLG DNWFGGSYET VAGTPKVITV KQGIEQKPVE QRIHFSKGNA
310 320 330 340 350
MSALAAHRVC GVPLETLARS RKPRDLTDDL SCAYQAQNIV SLFVATRILF
360 370 380 390 400
SHLDSVFTLN LDEQEPEVAE RLSDLRRINE NNPGMVTQVL TVARQIYNDY
410 420 430 440 450
VTHHPGLTPE QTSAGAQAAD ILSLFCPDAD KSCVASNNDQ ANINIESRSG
460 470 480 490 500
RSYLPENRAV ITPQGVTNWT YQELEATHQA LTREGYVFVG YHGTNHVAAQ
510 520 530 540 550
TIVNRIAPVP RGNNTENEEK WGGLYVATHA EVAHGYARIK EGTGEYGLPT
560 570 580 590 600
RAERDARGVM LRVYIPRASL ERFYRTNTPL ENAEEHITQV IGHSLPLRNE
610 620 630 640 650
AFTGPESAGG EDETVIGWDM AIHAVAIPST IPGNAYEELA IDEEAVAKEQ
660
SISTKPPYKE RKDELK
Length:666
Mass (Da):74,293
Last modified:March 15, 2005 - v1
Checksum:i3A76A86E0BC7FDD7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY876053 Genomic DNA. Translation: AAW80252.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY876053 Genomic DNA. Translation: AAW80252.1 .

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2Q5T X-ray 2.10 A 33-666 [» ]
2Q6M X-ray 1.25 A 459-666 [» ]
3ESS X-ray 1.19 A 459-665 [» ]
3KI0 X-ray 1.29 A 459-665 [» ]
3KI1 X-ray 1.43 A 459-665 [» ]
3KI2 X-ray 1.28 A 459-665 [» ]
3KI3 X-ray 1.27 A 459-665 [» ]
3KI4 X-ray 1.65 A 459-665 [» ]
3KI5 X-ray 1.55 A 459-665 [» ]
3KI6 X-ray 1.54 A 459-665 [» ]
3KI7 X-ray 1.32 A 459-665 [» ]
3NY6 X-ray 1.68 A 459-665 [» ]
3Q9O X-ray 1.79 A 33-666 [» ]
ProteinModelPortali Q5EK40.
SMRi Q5EK40. Positions 37-662.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei Q5EK40.

Family and domain databases

Gene3Di 2.60.120.200. 1 hit.
3.90.1350.10. 1 hit.
InterProi IPR013320. ConA-like_dom.
IPR015185. Exotox-A_bind.
IPR015099. Exotox-A_cataly_dom.
IPR015186. Exotox-A_middle_dom.
[Graphical view ]
Pfami PF09101. Exotox-A_bind. 1 hit.
PF09009. Exotox-A_cataly. 1 hit.
PF09102. Exotox-A_target. 1 hit.
[Graphical view ]
SUPFAMi SSF49899. SSF49899. 1 hit.
SSF56864. SSF56864. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "A glimpse into the expanded genome content of Vibrio cholerae through identification of genes present in environmental strains."
    Purdy A., Rohwer F., Edwards R., Azam F., Bartlett D.H.
    J. Bacteriol. 187:2992-3001(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: TP.
  2. Cited for: X-RAY CRYSTALLOGRAPHY (1.25 ANGSTROMS) OF 459-666 IN COMPLEX WITH INHIBITOR AND (2.1 ANGSTROMS) OF 33-666, FUNCTION AS A TOXIN, FUNCTION AS A GLYCOHYDROLASE, FUNCTION AS AN ADP-RIBOSYLTRANSFERASE, BIOPHYSICOCHEMICAL PROPERTIES, MUTAGENESIS OF GLU-613.
    Strain: TP.
  3. Cited for: X-RAY CRYSTALLOGRAPHY (1.19 ANGSTROMS) OF 459-665 IN COMPLEX WITH INHIBITOR, FUNCTION AS A TOXIN, ENZYME REGULATION, MUTAGENESIS OF GLU-606.
    Strain: TP.
  4. "Newly discovered and characterized antivirulence compounds inhibit bacterial mono-ADP-ribosyltransferase toxins."
    Turgeon Z., Jorgensen R., Visschedyk D., Edwards P.R., Legree S., McGregor C., Fieldhouse R.J., Mangroo D., Schapira M., Merrill A.R.
    Antimicrob. Agents Chemother. 55:983-991(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.27 ANGSTROMS) OF 459-665 IN COMPLEX WITH INHIBITORS.
    Strain: TP.

Entry informationi

Entry nameiCHXA_VIBCL
AccessioniPrimary (citable) accession number: Q5EK40
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 28, 2011
Last sequence update: March 15, 2005
Last modified: November 26, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Exotoxins are often highly strain specific; not encoded in strain ATCC 39315 / El Tor Inabe N16961.

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

External Data

Dasty 3