Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q5EK40

- CHXA_VIBCL

UniProt

Q5EK40 - CHXA_VIBCL

Protein

Cholix toxin

Gene

chxA

Organism
Vibrio cholerae
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 44 (01 Oct 2014)
      Sequence version 1 (15 Mar 2005)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    An NAD-dependent ADP-ribosyltransferase (ADPRT), it catalyzes the transfer of the ADP-ribosyl moiety of oxidized NAD onto eukaryotic elongation factor 2 (eEF-2) thus arresting protein synthesis. It probably uses the eukaryotic prolow-density lipoprotein receptor-related protein 1 (LRP1) to enter mouse cells, although there seems to be at least one other receptor as well. Is active against mouse fibroblasts, Chinese hamster ovary eEF-2, brine shrimp (Artemia spp. nauplii) and upon expression in S.cerevisiae.2 Publications

    Catalytic activityi

    NAD+ + diphthamide-[translation elongation factor 2] = nicotinamide + N-(ADP-D-ribosyl)diphthamide-[translation elongation factor 2].

    Enzyme regulationi

    Partially inhibited by 1,8-naphthalimide (NAP).1 Publication

    Kineticsi

    For ADP-ribosyltransferase activity of a catalytic fragment of residues 459-666.

    1. KM=45 µM for NAD1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei613 – 6131Curated

    GO - Molecular functioni

    1. NAD+-diphthamide ADP-ribosyltransferase activity Source: UniProtKB-EC

    Keywords - Molecular functioni

    Glycosyltransferase, Toxin, Transferase

    Keywords - Ligandi

    NAD

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cholix toxin (EC:2.4.2.36)
    Alternative name(s):
    Exotoxin A
    NAD(+)--diphthamide ADP-ribosyltransferase
    Gene namesi
    Name:chxA
    Synonyms:toxA
    OrganismiVibrio cholerae
    Taxonomic identifieri666 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi606 – 6061E → A: Loss of ADPRT activity, loss of toxicity against yeast. 1 Publication
    Mutagenesisi613 – 6131E → A: Loss of ADPRT activity, loss of toxicity against mouse cells, brine shrimp and yeast. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3232Sequence AnalysisAdd
    BLAST
    Chaini33 – 666634Cholix toxinPRO_0000409819Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi43 ↔ 47
    Disulfide bondi240 ↔ 257
    Disulfide bondi310 ↔ 332
    Disulfide bondi426 ↔ 433

    Post-translational modificationi

    Probably requires further proteolytic processing to generate a fragment with toxic activity.

    Keywords - PTMi

    Disulfide bond

    Structurei

    Secondary structure

    1
    666
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi39 – 424
    Beta strandi47 – 493
    Beta strandi57 – 626
    Beta strandi71 – 8111
    Beta strandi94 – 996
    Turni100 – 1023
    Beta strandi103 – 1086
    Beta strandi113 – 1153
    Beta strandi120 – 1267
    Beta strandi131 – 1377
    Beta strandi139 – 15214
    Beta strandi157 – 16610
    Beta strandi170 – 1723
    Beta strandi177 – 1826
    Helixi185 – 1906
    Turni191 – 1933
    Beta strandi194 – 20310
    Beta strandi208 – 21912
    Helixi226 – 2305
    Helixi231 – 2344
    Helixi236 – 2416
    Helixi245 – 2484
    Beta strandi269 – 2735
    Beta strandi289 – 2935
    Helixi300 – 31011
    Helixi314 – 3174
    Helixi328 – 34619
    Helixi350 – 3545
    Helixi355 – 3584
    Helixi361 – 3633
    Helixi366 – 38116
    Helixi385 – 40319
    Helixi409 – 41810
    Beta strandi420 – 4256
    Beta strandi441 – 4455
    Helixi450 – 4534
    Beta strandi460 – 4623
    Beta strandi465 – 4684
    Helixi471 – 48313
    Beta strandi486 – 4949
    Helixi496 – 5049
    Helixi516 – 5205
    Beta strandi523 – 5297
    Helixi530 – 5345
    Helixi545 – 5473
    Helixi551 – 5555
    Beta strandi559 – 5668
    Helixi567 – 5726
    Beta strandi573 – 5753
    Helixi580 – 5823
    Helixi584 – 5918
    Beta strandi601 – 6077
    Beta strandi612 – 6165
    Helixi618 – 6214
    Beta strandi625 – 6328
    Helixi643 – 6497
    Helixi650 – 6523
    Helixi662 – 6643

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2Q5TX-ray2.10A33-666[»]
    2Q6MX-ray1.25A459-666[»]
    3ESSX-ray1.19A459-665[»]
    3KI0X-ray1.29A459-665[»]
    3KI1X-ray1.43A459-665[»]
    3KI2X-ray1.28A459-665[»]
    3KI3X-ray1.27A459-665[»]
    3KI4X-ray1.65A459-665[»]
    3KI5X-ray1.55A459-665[»]
    3KI6X-ray1.54A459-665[»]
    3KI7X-ray1.32A459-665[»]
    3NY6X-ray1.68A459-665[»]
    3Q9OX-ray1.79A33-666[»]
    ProteinModelPortaliQ5EK40.
    SMRiQ5EK40. Positions 37-662.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ5EK40.

    Family & Domainsi

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di2.60.120.200. 1 hit.
    3.90.1350.10. 1 hit.
    InterProiIPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    IPR015185. Exotox-A_bind.
    IPR015099. Exotox-A_cataly_dom.
    IPR015186. Exotox-A_middle_dom.
    [Graphical view]
    PfamiPF09101. Exotox-A_bind. 1 hit.
    PF09009. Exotox-A_cataly. 1 hit.
    PF09102. Exotox-A_target. 1 hit.
    [Graphical view]
    SUPFAMiSSF49899. SSF49899. 1 hit.
    SSF56864. SSF56864. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q5EK40-1 [UniParc]FASTAAdd to Basket

    « Hide

    MYLTFYLEKV MKKMLLIAGA TVISSMAHPT FAVEDELNIF DECRSPCSLT    50
    PEPGKPIQSK LSIPSDVVLD EGVLYYSMTI NDEQNDIKDE DKGESIITIG 100
    EFATVRATRH YVNQDAPFGV IHLDITTENG TKTYSYNRKE GEFAINWLVP 150
    IGEDSPASIK ISVDELDQQR NIIEVPKLYS IDLDNQTLEQ WKTQGNVSFS 200
    VTRPEHNIAI SWPSVSYKAA QKEGSRHKRW AHWHTGLALC WLVPMDAIYN 250
    YITQQNCTLG DNWFGGSYET VAGTPKVITV KQGIEQKPVE QRIHFSKGNA 300
    MSALAAHRVC GVPLETLARS RKPRDLTDDL SCAYQAQNIV SLFVATRILF 350
    SHLDSVFTLN LDEQEPEVAE RLSDLRRINE NNPGMVTQVL TVARQIYNDY 400
    VTHHPGLTPE QTSAGAQAAD ILSLFCPDAD KSCVASNNDQ ANINIESRSG 450
    RSYLPENRAV ITPQGVTNWT YQELEATHQA LTREGYVFVG YHGTNHVAAQ 500
    TIVNRIAPVP RGNNTENEEK WGGLYVATHA EVAHGYARIK EGTGEYGLPT 550
    RAERDARGVM LRVYIPRASL ERFYRTNTPL ENAEEHITQV IGHSLPLRNE 600
    AFTGPESAGG EDETVIGWDM AIHAVAIPST IPGNAYEELA IDEEAVAKEQ 650
    SISTKPPYKE RKDELK 666
    Length:666
    Mass (Da):74,293
    Last modified:March 15, 2005 - v1
    Checksum:i3A76A86E0BC7FDD7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY876053 Genomic DNA. Translation: AAW80252.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY876053 Genomic DNA. Translation: AAW80252.1 .

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2Q5T X-ray 2.10 A 33-666 [» ]
    2Q6M X-ray 1.25 A 459-666 [» ]
    3ESS X-ray 1.19 A 459-665 [» ]
    3KI0 X-ray 1.29 A 459-665 [» ]
    3KI1 X-ray 1.43 A 459-665 [» ]
    3KI2 X-ray 1.28 A 459-665 [» ]
    3KI3 X-ray 1.27 A 459-665 [» ]
    3KI4 X-ray 1.65 A 459-665 [» ]
    3KI5 X-ray 1.55 A 459-665 [» ]
    3KI6 X-ray 1.54 A 459-665 [» ]
    3KI7 X-ray 1.32 A 459-665 [» ]
    3NY6 X-ray 1.68 A 459-665 [» ]
    3Q9O X-ray 1.79 A 33-666 [» ]
    ProteinModelPortali Q5EK40.
    SMRi Q5EK40. Positions 37-662.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei Q5EK40.

    Family and domain databases

    Gene3Di 2.60.120.200. 1 hit.
    3.90.1350.10. 1 hit.
    InterProi IPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    IPR015185. Exotox-A_bind.
    IPR015099. Exotox-A_cataly_dom.
    IPR015186. Exotox-A_middle_dom.
    [Graphical view ]
    Pfami PF09101. Exotox-A_bind. 1 hit.
    PF09009. Exotox-A_cataly. 1 hit.
    PF09102. Exotox-A_target. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49899. SSF49899. 1 hit.
    SSF56864. SSF56864. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "A glimpse into the expanded genome content of Vibrio cholerae through identification of genes present in environmental strains."
      Purdy A., Rohwer F., Edwards R., Azam F., Bartlett D.H.
      J. Bacteriol. 187:2992-3001(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: TP.
    2. Cited for: X-RAY CRYSTALLOGRAPHY (1.25 ANGSTROMS) OF 459-666 IN COMPLEX WITH INHIBITOR AND (2.1 ANGSTROMS) OF 33-666, FUNCTION AS A TOXIN, FUNCTION AS A GLYCOHYDROLASE, FUNCTION AS AN ADP-RIBOSYLTRANSFERASE, BIOPHYSICOCHEMICAL PROPERTIES, MUTAGENESIS OF GLU-613.
      Strain: TP.
    3. Cited for: X-RAY CRYSTALLOGRAPHY (1.19 ANGSTROMS) OF 459-665 IN COMPLEX WITH INHIBITOR, FUNCTION AS A TOXIN, ENZYME REGULATION, MUTAGENESIS OF GLU-606.
      Strain: TP.
    4. "Newly discovered and characterized antivirulence compounds inhibit bacterial mono-ADP-ribosyltransferase toxins."
      Turgeon Z., Jorgensen R., Visschedyk D., Edwards P.R., Legree S., McGregor C., Fieldhouse R.J., Mangroo D., Schapira M., Merrill A.R.
      Antimicrob. Agents Chemother. 55:983-991(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.27 ANGSTROMS) OF 459-665 IN COMPLEX WITH INHIBITORS.
      Strain: TP.

    Entry informationi

    Entry nameiCHXA_VIBCL
    AccessioniPrimary (citable) accession number: Q5EK40
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 28, 2011
    Last sequence update: March 15, 2005
    Last modified: October 1, 2014
    This is version 44 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Exotoxins are often highly strain specific; not encoded in strain ATCC 39315 / El Tor Inabe N16961.

    Keywords - Technical termi

    3D-structure

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references

    External Data

    Dasty 3