Q5EHP3 (CM3E_CONMR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Conotoxin mr3e Alternative name(s): Mr3.4 Mr3.7
Organism	Conus marmoreus (Marble cone)
Taxonomic identifier	42752 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Mollusca › Gastropoda › Caenogastropoda › Hypsogastropoda › Neogastropoda › Conoidea › Conidae › Conus

Protein attributes

Sequence length	71 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Intracranially injection into mice does not elicit symptoms.
Subcellular location	Secreted.
Tissue specificity	Expressed by the venom duct.
Domain	The cysteine framework is III (CC-C-C-CC). Classified in the M-1 branch, since 1 residue stands between the fourth and the fifth cysteine residues.
Sequence similarities	Belongs to the conotoxin M superfamily.
Mass spectrometry	Molecular mass is 1743.3 Da from positions 1 - 16. Determined by ESI. Ref.1

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Toxin
PTM	Amidation Disulfide bond
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	pathogenesis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ion channel inhibitor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 24

24

Potential

Propeptide

25 – 54

30

PRO_0000289866

Peptide

55 – 70

16

Conotoxin mr3e Ref.1

PRO_0000289867

Amino acid modifications

Modified residue

70

1

Aspartic acid 1-amide Ref.1

Disulfide bond

56 ↔ 68

Ref.1 Ref.2

Disulfide bond

57 ↔ 66

Ref.1 Ref.2

Disulfide bond

62 ↔ 69

Ref.1 Ref.2

Secondary structure

1

.

.

.

71

Helix Strand Turn

Details...

Turn

59 – 61

3

Sequences

	Sequence		Length	Mass (Da)
	Q5EHP3 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: D4EF684276F2A0D9	FASTA	71	7,976
	10 20 30 40 50 60 MLKMGVVLFI VLVLFPLATL QLDADQPVER YAENKRLLNP DERRGIILHA LGQRVCCPFG 70 GCHELCYCCD G

References

[1]

"Characterization of novel M-superfamily conotoxins with new disulfide linkage." Han Y.-H., Wang Q., Jiang H., Liu L., Xiao C., Yuan D.-D., Shao X.-X., Dai Q.-Y., Cheng J.-S., Chi C.-W. FEBS J. 273:4972-4982(2006) [PubMed: 17042781] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 55-70, AMIDATION AT ASP-70, DISULFIDE BONDS, MASS SPECTROMETRY, SYNTHESIS OF 55-70, BIOASSAY. Tissue: Venom and Venom duct.

[2]

"Solution structure of an M-1 conotoxin with a novel disulfide linkage." Du W.-H., Han Y.-H., Huang F.-J., Li J., Chi C.-W., Fang W.-H. FEBS J. 274:2596-2602(2007) [PubMed: 17437523] [Abstract] Cited for: STRUCTURE BY NMR OF 55-70, DISULFIDE BONDS.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AY880683 mRNA. Translation: AAW78560.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2EFZ	NMR	-	A	55-70	[»]

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Organism-specific databases

ConoServer

1081. MrIIIE precursor.

Family and domain databases

InterPro

IPR004214. Conotoxin.
[Graphical view]

Pfam

PF02950. Conotoxin. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

CM3E_CONMR

Accession

Primary (citable) accession number: Q5EHP3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	March 15, 2005
Last modified:	December 14, 2011
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Animal Toxin Annotation Program