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Protein

Caspase recruitment domain-containing protein 16

Gene

CARD16

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Caspase inhibitor. Acts as a regulator of procaspase-1/CASP1 activation implicated in the regulation of the proteolytic maturation of pro-interleukin-1 beta (IL1B) and its release during inflammation. Inhibits the release of IL1B in response to LPS in monocytes. Also induces NF-kappa-B activation during the pro-inflammatory cytokine response. Also able to inhibit CASP1-mediated neuronal cell death, TNF-alpha, hypoxia-, UV-, and staurosporine-mediated cell death but not ER stress-mediated cell death. Acts by preventing activation of caspases CASP1 and CASP4, possibly by preventing the interaction between CASP1 and RIPK2.3 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Thiol protease inhibitor

Names & Taxonomyi

Protein namesi
Recommended name:
Caspase recruitment domain-containing protein 16
Alternative name(s):
Caspase recruitment domain-only protein 1
Short name:
CARD-only protein 1
Caspase-1 inhibitor COP
Pseudo interleukin-1 beta converting enzyme
Short name:
Pseudo-ICE
Short name:
Pseudo-IL1B-converting enzyme
Gene namesi
Name:CARD16
Synonyms:COP, COP1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:33701. CARD16.

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA164717628.

Polymorphism and mutation databases

BioMutaiCARD16.
DMDMi74722547.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 197197Caspase recruitment domain-containing protein 16PRO_0000349180Add
BLAST

Proteomic databases

MaxQBiQ5EG05.
PaxDbiQ5EG05.
PeptideAtlasiQ5EG05.
PRIDEiQ5EG05.

Expressioni

Tissue specificityi

Widely expressed. Expressed at higher level in placenta, spleen, lymph node and bone marrow. Weakly or not expressed in thymus.2 Publications

Inductioni

Down-regulated in patients suffering of Huntington disease.1 Publication

Gene expression databases

BgeeiENSG00000204397.
CleanExiHS_CARD16.
ExpressionAtlasiQ5EG05. baseline and differential.
GenevisibleiQ5EG05. HS.

Organism-specific databases

HPAiHPA053981.

Interactioni

Subunit structurei

Homooligomer. Interacts with CASP1, CASP4, CARD8 and RIPK2.4 Publications

Protein-protein interaction databases

BioGridi125339. 3 interactions.
IntActiQ5EG05. 1 interaction.
MINTiMINT-231338.
STRINGi9606.ENSP00000364858.

Structurei

3D structure databases

ProteinModelPortaliQ5EG05.
SMRiQ5EG05. Positions 2-89, 99-127.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 9191CARDPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 CARD domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3573. Eukaryota.
ENOG410ZQIE. LUCA.
GeneTreeiENSGT00510000049857.
HOGENOMiHOG000111300.
HOVERGENiHBG006113.
InParanoidiQ5EG05.
OMAiRTNFIER.
OrthoDBiEOG091G0L5Y.
PhylomeDBiQ5EG05.
TreeFamiTF330675.

Family and domain databases

Gene3Di1.10.533.10. 1 hit.
InterProiIPR001315. CARD.
IPR011029. DEATH-like_dom.
[Graphical view]
PfamiPF00619. CARD. 1 hit.
[Graphical view]
SMARTiSM00114. CARD. 1 hit.
[Graphical view]
SUPFAMiSSF47986. SSF47986. 1 hit.
PROSITEiPS50209. CARD. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q5EG05-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MADKVLKEKR KLFIHSMGEG TINGLLDELL QTRVLNQEEM EKVKRENATV
60 70 80 90 100
MDKTRALIDS VIPKGAQACQ ICITYICEED SYLAETLGLS AALQAVQDNP
110 120 130 140 150
AMPTCSSPEG RIKLCFLEDA QRIWKQKLQR CHVQNTIIKW SERYTSGSFE
160 170 180 190
MQWLFLRTNF IERFWRNILL LPLHKGSLYP RIPGLGKELQ TGTHKLS
Length:197
Mass (Da):22,625
Last modified:March 15, 2005 - v1
Checksum:i5DCAC6A9B2FAE82F
GO
Isoform 2 (identifier: Q5EG05-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     92-197: ALQAVQDNPA...ELQTGTHKLS → GPIPGN

Show »
Length:97
Mass (Da):10,737
Checksum:i8697C3B5706B9B91
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti33 – 331R → S.
Corresponds to variant rs35966314 [ dbSNP | Ensembl ].
VAR_046279
Natural varianti37 – 371Q → K.
Corresponds to variant rs1042744 [ dbSNP | Ensembl ].
VAR_046280
Natural varianti56 – 561A → D.
Corresponds to variant rs34534919 [ dbSNP | Ensembl ].
VAR_046281
Natural varianti167 – 1671N → I.
Corresponds to variant rs542571 [ dbSNP | Ensembl ].
VAR_046282

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei92 – 197106ALQAV…THKLS → GPIPGN in isoform 2. 3 PublicationsVSP_035216Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF367017 mRNA. Translation: AAK71682.1.
AY885669 mRNA. Translation: AAW78563.1.
AK311902 mRNA. Translation: BAG34843.1.
CH471065 Genomic DNA. Translation: EAW67062.1.
BC117478 mRNA. Translation: AAI17479.1.
BC117480 mRNA. Translation: AAI17481.1.
CCDSiCCDS31661.1. [Q5EG05-1]
CCDS41705.1. [Q5EG05-2]
RefSeqiNP_001017534.1. NM_001017534.1. [Q5EG05-1]
NP_443121.1. NM_052889.2. [Q5EG05-2]
UniGeneiHs.348365.

Genome annotation databases

EnsembliENST00000375704; ENSP00000364856; ENSG00000204397. [Q5EG05-2]
ENST00000375706; ENSP00000364858; ENSG00000204397. [Q5EG05-1]
ENST00000525374; ENSP00000433700; ENSG00000204397. [Q5EG05-2]
GeneIDi114769.
KEGGihsa:114769.
UCSCiuc001pio.2. human. [Q5EG05-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF367017 mRNA. Translation: AAK71682.1.
AY885669 mRNA. Translation: AAW78563.1.
AK311902 mRNA. Translation: BAG34843.1.
CH471065 Genomic DNA. Translation: EAW67062.1.
BC117478 mRNA. Translation: AAI17479.1.
BC117480 mRNA. Translation: AAI17481.1.
CCDSiCCDS31661.1. [Q5EG05-1]
CCDS41705.1. [Q5EG05-2]
RefSeqiNP_001017534.1. NM_001017534.1. [Q5EG05-1]
NP_443121.1. NM_052889.2. [Q5EG05-2]
UniGeneiHs.348365.

3D structure databases

ProteinModelPortaliQ5EG05.
SMRiQ5EG05. Positions 2-89, 99-127.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi125339. 3 interactions.
IntActiQ5EG05. 1 interaction.
MINTiMINT-231338.
STRINGi9606.ENSP00000364858.

Polymorphism and mutation databases

BioMutaiCARD16.
DMDMi74722547.

Proteomic databases

MaxQBiQ5EG05.
PaxDbiQ5EG05.
PeptideAtlasiQ5EG05.
PRIDEiQ5EG05.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000375704; ENSP00000364856; ENSG00000204397. [Q5EG05-2]
ENST00000375706; ENSP00000364858; ENSG00000204397. [Q5EG05-1]
ENST00000525374; ENSP00000433700; ENSG00000204397. [Q5EG05-2]
GeneIDi114769.
KEGGihsa:114769.
UCSCiuc001pio.2. human. [Q5EG05-1]

Organism-specific databases

CTDi114769.
GeneCardsiCARD16.
HGNCiHGNC:33701. CARD16.
HPAiHPA053981.
MIMi615680. gene.
neXtProtiNX_Q5EG05.
PharmGKBiPA164717628.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3573. Eukaryota.
ENOG410ZQIE. LUCA.
GeneTreeiENSGT00510000049857.
HOGENOMiHOG000111300.
HOVERGENiHBG006113.
InParanoidiQ5EG05.
OMAiRTNFIER.
OrthoDBiEOG091G0L5Y.
PhylomeDBiQ5EG05.
TreeFamiTF330675.

Miscellaneous databases

GeneWikiiCOP1.
GenomeRNAii114769.
PROiQ5EG05.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000204397.
CleanExiHS_CARD16.
ExpressionAtlasiQ5EG05. baseline and differential.
GenevisibleiQ5EG05. HS.

Family and domain databases

Gene3Di1.10.533.10. 1 hit.
InterProiIPR001315. CARD.
IPR011029. DEATH-like_dom.
[Graphical view]
PfamiPF00619. CARD. 1 hit.
[Graphical view]
SMARTiSM00114. CARD. 1 hit.
[Graphical view]
SUPFAMiSSF47986. SSF47986. 1 hit.
PROSITEiPS50209. CARD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCAR16_HUMAN
AccessioniPrimary (citable) accession number: Q5EG05
Secondary accession number(s): Q96RJ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: March 15, 2005
Last modified: September 7, 2016
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.