Reviewed, UniProtKB/Swiss-Prot Q5EFU4 (CRD1_HORVU)

Names and origin

Protein names

Recommended name:
    Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase, chloroplastic
      Short name=Mg-protoporphyrin IX monomethyl ester oxidative cyclase
    EC=1.14.13.81
Alternative name(s):
    Protein Xantha-l

Gene names

Name:

CRD1

Organism

Hordeum vulgare (Barley)

Taxonomic identifier

4513 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Pooideae › Triticeae › Hordeum

Protein attributes

Sequence length	417 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the formation of the isocyclic ring in chlorophyll biosynthesis. Mediates the cyclase reaction, which results in the formation of divinylprotochlorophyllide (Pchlide) characteristic of all chlorophylls from magnesium-protoporphyrin IX 13-monomethyl ester (MgPMME).
Catalytic activity	Magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O(2) = 13(1)-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADP(+) + H(2)O. 13(1)-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O(2) = 13(1)-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADP(+) + 2 H(2)O. 13(1)-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O(2) = divinylprotochlorophyllide + NADP(+) + 2 H(2)O.
Cofactor	Iron By similarity.
Pathway	Porphyrin biosynthesis; chlorophyll biosynthesis.
Subcellular location	Plastid › chloroplast membrane; Peripheral membrane proteinPotential.
Sequence similarities	Belongs to the acsF family.

Ontologies

Keywords
Biological process	Chlorophyll biosynthesis Photosynthesis
Cellular component	Chloroplast Membrane Plastid
Domain	Transit peptide
Ligand	Iron Metal-binding NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	chlorophyll biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW photosynthesis Inferred from electronic annotation. Source: InterPro
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-KW membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 45	45	Chloroplast Potential
	Chain	46 – 417	372	Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase, chloroplastic		PRO_0000000603
Experimental info
	Mutagenesis	154	1	G → E in xantha-l(81); induces accumulation of MgPMME
	Mutagenesis	180	1	S → F in xantha-l(35); induces accumulation of MgPMME

Sequences

	Sequence		Length	Mass (Da)
	Q5EFU4-1 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: 83933398C325E77E	FASTA	417	48,161
	10 20 30 40 50 60 MASAMELSLL NPAMHHYGIA AKTASHLPVV PARRASSGAV RFRVRAAAAA PPAPAAKPGS 70 80 90 100 110 120 PKKRGKTEVN ESLLTPRFYT TDFDEMEQLF NAEINKQLNQ DEFDALLQEF KTDYNQTHFI 130 140 150 160 170 180 RNPEFKEAAD KMQGPLRQIF VEFLERSCTA EFSGFLLYKE LGRRLKKTNP VVAEIFSLMS 190 200 210 220 230 240 RDEARHAGFL NKGLSDFNLA LDLGFLTKAR KYTFFKPKFI FYATYLSEKI GYWRYITIFR 250 260 270 280 290 300 HLKANPEYQV YPIFKYFENW CQDENRHGDF FSALLKAQPQ FLNDWKAKLW SRFFCLSVYI 310 320 330 340 350 360 TMYLNDCQRS AFYEGIGLNT KEFDMHVIYE TNRTTARIFP AVPDVENPEF KRKLDRMVDI 370 380 390 400 410 NLKIISIGES NDLPLVKNLK RVPLIAQLVS EIIAAYLMPP IESGSVDFAE FEPKLVY

References

[1]

"Xantha-l encodes a membrane subunit of the aerobic Mg-protoporphyrin IX monomethyl ester cyclase involved in chlorophyll biosynthesis." Rzeznicka K., Walker C.J., Westergren T., Kannangara C.G., von Wettstein D., Merchant S., Gough S.P., Hansson M. Proc. Natl. Acad. Sci. U.S.A. 102:5886-5891(2005) [PubMed: 15824317] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ENZYME ACTIVITY, PATHWAY, FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF GLY-154 AND SER-180.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY887063 Genomic DNA. Translation: AAW80518.1.
3D structure databases
ModBase	Search...
Organism-specific databases
Gramene	Q5EFU4.
Family and domain databases
InterPro	IPR008434. AcsF. IPR003251. Rubrerythrin. [Graphical view]
Pfam	PF02915. Rubrerythrin. 1 hit. [Graphical view]
TIGRFAMs	TIGR02029. AcsF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CRD1_HORVU

Accession

Primary (citable) accession number: Q5EFU4

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2005
Last sequence update:	March 15, 2005
Last modified:	November 25, 2008
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)