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Q5EF78 (ICA_APICA) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 22. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Icarapin
Alternative name(s):
Venom protein 2
Allergen=Api m 10
OrganismApis mellifera carnica (Carniolan bee)
Taxonomic identifier88217 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaHymenopteraApocritaAculeataApoideaApidaeApis

Protein attributes

Sequence length223 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Subcellular location

Secreted Ref.1 Ref.2.

Tissue specificity

Expressed by the venom duct. Ref.1 Ref.2

Allergenic properties

Causes an allergic reaction in human. Ref.2

Caution

Sequences and experimental results obtained from Ref.1 and Ref.2 are obtained from A.mellifera carnica and not A.mellifera.

Ontologies

Keywords
   Cellular componentSecreted
   Coding sequence diversityAlternative splicing
   DiseaseAllergen
   DomainSignal
   PTMGlycoprotein
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform variant 1 (identifier: Q5EF78-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform variant 2 (identifier: Q5EF78-2)

The sequence of this isoform differs from the canonical sequence as follows:
     90-94: SAISA → T
Note: fragment, sequence of 45-223.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919 Potential
Chain20 – 223204Icarapin
PRO_5000095342

Amino acid modifications

Glycosylation1261N-linked (GlcNAc...) Potential
Glycosylation1421N-linked (GlcNAc...) Potential
Glycosylation1681N-linked (GlcNAc...) Potential
Glycosylation1931N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence90 – 945SAISA → T in isoform variant 2.
VSP_040198

Experimental info

Sequence conflict1401T → A in AAW81036. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform variant 1 [UniParc].

Last modified November 30, 2010. Version 2.
Checksum: 9BD51D084DFBFB28

FASTA22324,819
        10         20         30         40         50         60 
MKTLGVLFIA AWFIACTHSF PGAHDEDSKE ERKNVDTVLV LPSIERDQMM AATFDFPSLS 

        70         80         90        100        110        120 
FEDSDEGSNW NWNTLLRPNF LDGWYQTLQS AISAHMKKVR EQMAGILSRI PEQGVVNWNK 

       130        140        150        160        170        180 
IPEGANTTST TKIIDGHVVT INETTYTDGS DDYSTLIRVR VIDVRPQNET ILTTVSSEAD 

       190        200        210        220 
SDVTTLPTLI GKNETSTQSS RSVESVEDFD NEIPKNQGDV LTA 

« Hide

Isoform variant 2 [UniParc].

Checksum: D0441FFB07784974
Show »

FASTA21924,490

References

[1]"The protein composition of honeybee venom reconsidered by a proteomic approach."
Peiren N., Vanrobaeys F., de Graaf D.C., Devreese B., Van Beeumen J., Jacobs F.J.
Biochim. Biophys. Acta 1752:1-5(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 33-46 AND 99-109, SUBCELLULAR LOCATION, TISSUE SPECIFICITY (ISOFORM VARIANT 1).
Tissue: Venom and Venom duct.
[2]"Molecular cloning and expression of icarapin, a novel IgE-binding bee venom protein."
Peiren N., de Graaf D.C., Brunain M., Bridts C.H., Ebo D.G., Stevens W.J., Jacobs F.J.
FEBS Lett. 580:4895-4899(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, ALTERNATIVE SPLICING, TISSUE SPECIFICITY, ALLERGEN (ISOFORMS VARIANT 1 AND VARIANT 2).
Tissue: Venom duct.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY897570 mRNA. Translation: AAW81036.1.
DQ485318 mRNA. Translation: ABF21077.1.
DQ485319 mRNA. Translation: ABF21078.1.
UniGeneAme.1095.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Proteomic databases

PaxDbQ5EF78.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

eggNOGNOG83101.
InParanoidQ5EF78.

Family and domain databases

ProtoNetSearch...

Entry information

Entry nameICA_APICA
AccessionPrimary (citable) accession number: Q5EF78
Secondary accession number(s): Q1HHN7
Entry history
Integrated into UniProtKB/Swiss-Prot: November 30, 2010
Last sequence update: November 30, 2010
Last modified: October 16, 2013
This is version 22 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

Allergens

Nomenclature of allergens and list of entries