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Protein

Icarapin

Gene
N/A
Organism
Apis mellifera carnica (Carniolan honeybee)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Names & Taxonomyi

Protein namesi
Recommended name:
Icarapin
Alternative name(s):
Venom protein 2
Allergen: Api m 10
OrganismiApis mellifera carnica (Carniolan honeybee)
Taxonomic identifieri88217 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaHymenopteraApocritaAculeataApoideaApidaeApis

Subcellular locationi

Secreted 2 Publications

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Allergenic propertiesi

Causes an allergic reaction in human.

Keywords - Diseasei

Allergen

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Sequence AnalysisAdd
BLAST
Chaini20 – 223204IcarapinPRO_5000095342Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi126 – 1261N-linked (GlcNAc...)Sequence Analysis
Glycosylationi142 – 1421N-linked (GlcNAc...)Sequence Analysis
Glycosylationi168 – 1681N-linked (GlcNAc...)Sequence Analysis
Glycosylationi193 – 1931N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ5EF78.

Expressioni

Tissue specificityi

Expressed by the venom duct.1 Publication

Family & Domainsi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG83101.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform variant 1 (identifier: Q5EF78-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKTLGVLFIA AWFIACTHSF PGAHDEDSKE ERKNVDTVLV LPSIERDQMM
60 70 80 90 100
AATFDFPSLS FEDSDEGSNW NWNTLLRPNF LDGWYQTLQS AISAHMKKVR
110 120 130 140 150
EQMAGILSRI PEQGVVNWNK IPEGANTTST TKIIDGHVVT INETTYTDGS
160 170 180 190 200
DDYSTLIRVR VIDVRPQNET ILTTVSSEAD SDVTTLPTLI GKNETSTQSS
210 220
RSVESVEDFD NEIPKNQGDV LTA
Length:223
Mass (Da):24,819
Last modified:November 30, 2010 - v2
Checksum:i9BD51D084DFBFB28
GO
Isoform variant 2 (identifier: Q5EF78-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     90-94: SAISA → T

Note: fragment, sequence of 45-223.

Show »
Length:219
Mass (Da):24,490
Checksum:iD0441FFB07784974
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti140 – 1401T → A in AAW81036 (PubMed:16914147).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei90 – 945SAISA → T in isoform variant 2. CuratedVSP_040198

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY897570 mRNA. Translation: AAW81036.1.
DQ485318 mRNA. Translation: ABF21077.1.
DQ485319 mRNA. Translation: ABF21078.1.
UniGeneiAme.1095.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY897570 mRNA. Translation: AAW81036.1.
DQ485318 mRNA. Translation: ABF21077.1.
DQ485319 mRNA. Translation: ABF21078.1.
UniGeneiAme.1095.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PaxDbiQ5EF78.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiNOG83101.

Family and domain databases

ProtoNetiSearch...

Publicationsi

  1. "The protein composition of honeybee venom reconsidered by a proteomic approach."
    Peiren N., Vanrobaeys F., de Graaf D.C., Devreese B., Van Beeumen J., Jacobs F.J.
    Biochim. Biophys. Acta 1752:1-5(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 33-46 AND 99-109, SUBCELLULAR LOCATION, TISSUE SPECIFICITY (ISOFORM VARIANT 1).
    Tissue: Venom and Venom duct.
  2. "Molecular cloning and expression of icarapin, a novel IgE-binding bee venom protein."
    Peiren N., de Graaf D.C., Brunain M., Bridts C.H., Ebo D.G., Stevens W.J., Jacobs F.J.
    FEBS Lett. 580:4895-4899(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, ALTERNATIVE SPLICING, TISSUE SPECIFICITY, ALLERGEN (ISOFORMS VARIANT 1 AND VARIANT 2).
    Tissue: Venom duct.

Entry informationi

Entry nameiICA_APICA
AccessioniPrimary (citable) accession number: Q5EF78
Secondary accession number(s): Q1HHN7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 30, 2010
Last sequence update: November 30, 2010
Last modified: January 7, 2015
This is version 25 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Caution

Sequences and experimental results obtained from PubMed:16112630 and PubMed:16914147 are obtained from A.mellifera carnica and not A.mellifera.Curated

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Allergens
    Nomenclature of allergens and list of entries

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.