Q5EF78 (ICA_APICA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Icarapin Alternative name(s): Venom protein 2 Allergen=Api m 10
Organism	Apis mellifera carnica (Carniolan bee)
Taxonomic identifier	88217 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Hymenoptera › Apocrita › Aculeata › Apoidea › Apidae › Apinae › Apini › Apis › Apis mellifera

Protein attributes

Sequence length	223 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Secreted Ref.1 Ref.2.
Tissue specificity	Expressed by the venom duct. Ref.1 Ref.2
Allergenic properties	Causes an allergic reaction in human. Ref.2
Caution	Sequences and experimental results obtained from Ref.1 and Ref.2 are obtained from A.mellifera carnica and not A.mellifera.

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Alternative splicing
Disease	Allergen
Domain	Signal
PTM	Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform variant 1 (identifier: Q5EF78-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform variant 2 (identifier: Q5EF78-2)

The sequence of this isoform differs from the canonical sequence as follows:
     90-94: SAISA → T

Note: fragment, sequence of 45-223.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Chain	20 – 223	204	Icarapin		PRO_5000095342
Amino acid modifications
	Glycosylation	126	1	N-linked (GlcNAc...) Potential
	Glycosylation	142	1	N-linked (GlcNAc...) Potential
	Glycosylation	168	1	N-linked (GlcNAc...) Potential
	Glycosylation	193	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	90 – 94	5	SAISA → T in isoform variant 2.		VSP_040198
Experimental info
	Sequence conflict	140	1	T → A in AAW81036. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform variant 1 [UniParc]. Last modified November 30, 2010. Version 2. Checksum: 9BD51D084DFBFB28	FASTA	223	24,819
	10 20 30 40 50 60 MKTLGVLFIA AWFIACTHSF PGAHDEDSKE ERKNVDTVLV LPSIERDQMM AATFDFPSLS 70 80 90 100 110 120 FEDSDEGSNW NWNTLLRPNF LDGWYQTLQS AISAHMKKVR EQMAGILSRI PEQGVVNWNK 130 140 150 160 170 180 IPEGANTTST TKIIDGHVVT INETTYTDGS DDYSTLIRVR VIDVRPQNET ILTTVSSEAD 190 200 210 220 SDVTTLPTLI GKNETSTQSS RSVESVEDFD NEIPKNQGDV LTA
	Isoform variant 2 [UniParc]. Checksum: D0441FFB07784974	FASTA	219	24,490
	10 20 30 40 50 60 MKTLGVLFIA AWFIACTHSF PGAHDEDSKE ERKNVDTVLV LPSIERDQMM AATFDFPSLS 70 80 90 100 110 120 FEDSDEGSNW NWNTLLRPNF LDGWYQTLQT HMKKVREQMA GILSRIPEQG VVNWNKIPEG 130 140 150 160 170 180 ANTTSTTKII DGHVVTINET TYTDGSDDYS TLIRVRVIDV RPQNETILTT VSSEADSDVT 190 200 210 TLPTLIGKNE TSTQSSRSVE SVEDFDNEIP KNQGDVLTA

References

[1]	"The protein composition of honeybee venom reconsidered by a proteomic approach." Peiren N., Vanrobaeys F., de Graaf D.C., Devreese B., Van Beeumen J., Jacobs F.J. Biochim. Biophys. Acta 1752:1-5(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 33-46 AND 99-109, SUBCELLULAR LOCATION, TISSUE SPECIFICITY (ISOFORM VARIANT 1). Tissue: Venom and Venom duct.
[2]	"Molecular cloning and expression of icarapin, a novel IgE-binding bee venom protein." Peiren N., de Graaf D.C., Brunain M., Bridts C.H., Ebo D.G., Stevens W.J., Jacobs F.J. FEBS Lett. 580:4895-4899(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, ALTERNATIVE SPLICING, TISSUE SPECIFICITY, ALLERGEN (ISOFORMS VARIANT 1 AND VARIANT 2). Tissue: Venom duct.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY897570 mRNA. Translation: AAW81036.1. DQ485318 mRNA. Translation: ABF21077.1. DQ485319 mRNA. Translation: ABF21078.1.
UniGene	Ame.1095.
3D structure databases
ModBase	Search...
Protein family/group databases
Allergome	8204. Api m 10. 8205. Api m 10.0101.
Proteomic databases
PaxDb	Q5EF78.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
eggNOG	NOG83101.
InParanoid	Q5EF78.
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

ICA_APICA

Accession

Primary (citable) accession number: Q5EF78
Secondary accession number(s): Q1HHN7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 30, 2010
Last sequence update:	November 30, 2010
Last modified:	November 28, 2012
This is version 21 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)