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Protein

Proton-coupled folate transporter

Gene

Slc46a1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Has been shown to act both as an intestinal proton-coupled high-affinity folate transporter and as an intestinal heme transporter which mediates heme uptake from the gut lumen into duodenal epithelial cells. The iron is then released from heme and may be transported into the bloodstream. Dietary heme iron is an important nutritional source of iron. Shows a higher affinity for folate than heme (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transport

Keywords - Ligandi

Folate-binding

Enzyme and pathway databases

ReactomeiR-RNO-196757. Metabolism of folate and pterines.
R-RNO-917937. Iron uptake and transport.

Names & Taxonomyi

Protein namesi
Recommended name:
Proton-coupled folate transporter
Alternative name(s):
Heme carrier protein 1
PCFT/HCP1
Solute carrier family 46 member 1
Gene namesi
Name:Slc46a1
Synonyms:Hcp1, Pcft
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi1309472. Slc46a1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2424CytoplasmicSequence analysisAdd
BLAST
Transmembranei25 – 4824HelicalSequence analysisAdd
BLAST
Topological domaini49 – 8436ExtracellularSequence analysisAdd
BLAST
Transmembranei85 – 10723HelicalSequence analysisAdd
BLAST
Topological domaini108 – 1136CytoplasmicSequence analysis
Transmembranei114 – 13724HelicalSequence analysisAdd
BLAST
Topological domaini138 – 1458ExtracellularSequence analysis
Transmembranei146 – 16823HelicalSequence analysisAdd
BLAST
Topological domaini169 – 18012CytoplasmicSequence analysisAdd
BLAST
Transmembranei181 – 20323HelicalSequence analysisAdd
BLAST
Topological domaini204 – 2129ExtracellularSequence analysis
Transmembranei213 – 23624HelicalSequence analysisAdd
BLAST
Topological domaini237 – 26529CytoplasmicSequence analysisAdd
BLAST
Transmembranei266 – 28823HelicalSequence analysisAdd
BLAST
Topological domaini289 – 30921ExtracellularSequence analysisAdd
BLAST
Transmembranei310 – 32819HelicalSequence analysisAdd
BLAST
Topological domaini329 – 3313CytoplasmicSequence analysis
Transmembranei332 – 35625HelicalSequence analysisAdd
BLAST
Topological domaini357 – 3593ExtracellularSequence analysis
Transmembranei360 – 38122HelicalSequence analysisAdd
BLAST
Topological domaini382 – 39312CytoplasmicSequence analysisAdd
BLAST
Transmembranei394 – 41219HelicalSequence analysisAdd
BLAST
Topological domaini413 – 42412ExtracellularSequence analysisAdd
BLAST
Transmembranei425 – 44925HelicalSequence analysisAdd
BLAST
Topological domaini450 – 45910CytoplasmicSequence analysis

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 459459Proton-coupled folate transporterPRO_0000084853Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei6 – 61PhosphoserineBy similarity
Glycosylationi58 – 581N-linked (GlcNAc...)Sequence analysis
Glycosylationi68 – 681N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Acetylation, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ5EBA8.
PRIDEiQ5EBA8.

PTM databases

iPTMnetiQ5EBA8.
PhosphoSiteiQ5EBA8.

Expressioni

Tissue specificityi

Expressed at low level in liver.1 Publication

Gene expression databases

GenevisibleiQ5EBA8. RN.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000013698.

Structurei

3D structure databases

ProteinModelPortaliQ5EBA8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2816. Eukaryota.
ENOG410ZVCA. LUCA.
GeneTreeiENSGT00530000063076.
HOGENOMiHOG000054191.
HOVERGENiHBG055334.
InParanoidiQ5EBA8.
KOiK14613.
OMAiTRHRENC.
OrthoDBiEOG7DNNVC.
PhylomeDBiQ5EBA8.
TreeFamiTF315701.

Family and domain databases

InterProiIPR011701. MFS.
IPR020846. MFS_dom.
IPR005829. Sugar_transporter_CS.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5EBA8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEGRVSPVGS SHRLLTAAVL FRGPVEPLVF LANFALVLQG PLTTQYIWHR
60 70 80 90 100
ISTELGYNGT RHRENCGNQS ADPVLKEVET LTSHWTLYMN VGGFLVGLFW
110 120 130 140 150
STLLGAWSDR VGRRPLLVLA SLGLLLQAVV SIFVVQLQLH IGFFVLGRAL
160 170 180 190 200
CALLGDFNGL LAASFASVAD VSSNHSRTFR MALLEACIGV AGTLASLLGG
210 220 230 240 250
HWLRAQGYAN PFWLALAVLI VMTLYAAFCF GETVKEPKST RLFTLRHHRS
260 270 280 290 300
IVQLYVVPAP EKSRMHLALY SLAIFVVVTV HFGAQDILTL YELSTPLCWD
310 320 330 340 350
SKLIGYGSAA QHLPYLTSLL GLRLLQFCLA DTWVAEIGLA FNILGMVVFA
360 370 380 390 400
FATITPLMFT GYGLLFLSLV TTPVIRAKLS KLVSESEQGA LFSAVACVNS
410 420 430 440 450
LAMLMASGIF NSLYPATLNF MKGFPFLLGA GLLFIPAILI GVLEKVNPHP

EFQQFPQNS
Length:459
Mass (Da):50,095
Last modified:March 15, 2005 - v1
Checksum:i4F590DA8DE7D6FA7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC089868 mRNA. Translation: AAH89868.1.
RefSeqiNP_001013991.1. NM_001013969.1.
XP_006247001.1. XM_006246939.2.
UniGeneiRn.153975.

Genome annotation databases

EnsembliENSRNOT00000013698; ENSRNOP00000013698; ENSRNOG00000010291.
GeneIDi303333.
KEGGirno:303333.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC089868 mRNA. Translation: AAH89868.1.
RefSeqiNP_001013991.1. NM_001013969.1.
XP_006247001.1. XM_006246939.2.
UniGeneiRn.153975.

3D structure databases

ProteinModelPortaliQ5EBA8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000013698.

PTM databases

iPTMnetiQ5EBA8.
PhosphoSiteiQ5EBA8.

Proteomic databases

PaxDbiQ5EBA8.
PRIDEiQ5EBA8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000013698; ENSRNOP00000013698; ENSRNOG00000010291.
GeneIDi303333.
KEGGirno:303333.

Organism-specific databases

CTDi113235.
RGDi1309472. Slc46a1.

Phylogenomic databases

eggNOGiKOG2816. Eukaryota.
ENOG410ZVCA. LUCA.
GeneTreeiENSGT00530000063076.
HOGENOMiHOG000054191.
HOVERGENiHBG055334.
InParanoidiQ5EBA8.
KOiK14613.
OMAiTRHRENC.
OrthoDBiEOG7DNNVC.
PhylomeDBiQ5EBA8.
TreeFamiTF315701.

Enzyme and pathway databases

ReactomeiR-RNO-196757. Metabolism of folate and pterines.
R-RNO-917937. Iron uptake and transport.

Miscellaneous databases

PROiQ5EBA8.

Gene expression databases

GenevisibleiQ5EBA8. RN.

Family and domain databases

InterProiIPR011701. MFS.
IPR020846. MFS_dom.
IPR005829. Sugar_transporter_CS.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Liver.
  2. Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiPCFT_RAT
AccessioniPrimary (citable) accession number: Q5EBA8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: March 15, 2005
Last modified: July 6, 2016
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.