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Protein

Transcription factor Spi-B

Gene

Spib

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Sequence specific transcriptional activator which binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. Promotes development of plasmacytoid dendritic cells (pDCs), also known as type 2 DC precursors (pre-DC2) or natural interferon (IFN)-producing cells. These cells have the capacity to produce large amounts of interferon and block viral replication. Required for B-cell receptor (BCR) signaling, which is necessary for normal B-cell development and antigenic stimulation (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi176 – 25984ETSPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor Spi-B
Gene namesi
Name:Spib
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi1565899. Spib.

Subcellular locationi

  • Nucleus By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 269269Transcription factor Spi-BPRO_0000204138Add
BLAST

Proteomic databases

PaxDbiQ5EBA3.
PRIDEiQ5EBA3.

Expressioni

Gene expression databases

BgeeiENSRNOG00000019660.

Interactioni

Subunit structurei

Can form homotypic interactions. Interacts with IRF4. May also interact with CREBBP, EP300, SPI1/SPI-1/PU.1 related, JUN and TBP (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000026599.

Structurei

3D structure databases

ProteinModelPortaliQ5EBA3.
SMRiQ5EBA3. Positions 175-263.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 3232TAD1 (Acidic)Add
BLAST
Regioni42 – 6322TAD2Add
BLAST

Domaini

The protein contains a weakly acidic N-terminal transactivation domain (TAD) followed by a second TAD rich in proline, serine and threonine. Each of these domains may be required for transcriptional activation of a subset of target genes (By similarity).By similarity

Sequence similaritiesi

Belongs to the ETS family.Curated
Contains 1 ETS DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3805. Eukaryota.
ENOG410XSXU. LUCA.
HOGENOMiHOG000095520.
HOVERGENiHBG002474.
InParanoidiQ5EBA3.
KOiK09439.
OrthoDBiEOG091G0DTY.
PhylomeDBiQ5EBA3.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR000418. Ets_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00178. Ets. 1 hit.
[Graphical view]
PRINTSiPR00454. ETSDOMAIN.
SMARTiSM00413. ETS. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
PROSITEiPS00345. ETS_DOMAIN_1. 1 hit.
PS00346. ETS_DOMAIN_2. 1 hit.
PS50061. ETS_DOMAIN_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5EBA3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLALEAAQLD GPHLSCLQYP EGVFYDLDSC KSFSYPDSDG GPDSTWGWTE
60 70 80 90 100
APPAPAIAAY EAFDPAATAA FAHTQAVQLC YGHGPSPSTY SPVGTLDPAP
110 120 130 140 150
SLEASGPGLQ VYPSEDFTSQ TLGSLAYAPY PSPVLSEEED ILLDSPALEV
160 170 180 190 200
SDSESDEALL AGSEGRGSEA GARKKLRLYQ FLLELLLRGD MRECVWWVEP
210 220 230 240 250
GAGVFQFSSK HKELLARRWG QQKGNRKRMT YQKLARALRN YAKTGEIRKV
260
KRKLTYQFDS ALLPAARRA
Length:269
Mass (Da):29,400
Last modified:March 15, 2005 - v1
Checksum:iB29710EFFEF069B8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC089881 mRNA. Translation: AAH89881.1.
RefSeqiNP_001019457.1. NM_001024286.1.
UniGeneiRn.18257.

Genome annotation databases

GeneIDi499146.
KEGGirno:499146.
UCSCiRGD:1565899. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC089881 mRNA. Translation: AAH89881.1.
RefSeqiNP_001019457.1. NM_001024286.1.
UniGeneiRn.18257.

3D structure databases

ProteinModelPortaliQ5EBA3.
SMRiQ5EBA3. Positions 175-263.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000026599.

Proteomic databases

PaxDbiQ5EBA3.
PRIDEiQ5EBA3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi499146.
KEGGirno:499146.
UCSCiRGD:1565899. rat.

Organism-specific databases

CTDi6689.
RGDi1565899. Spib.

Phylogenomic databases

eggNOGiKOG3805. Eukaryota.
ENOG410XSXU. LUCA.
HOGENOMiHOG000095520.
HOVERGENiHBG002474.
InParanoidiQ5EBA3.
KOiK09439.
OrthoDBiEOG091G0DTY.
PhylomeDBiQ5EBA3.

Miscellaneous databases

PROiQ5EBA3.

Gene expression databases

BgeeiENSRNOG00000019660.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR000418. Ets_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00178. Ets. 1 hit.
[Graphical view]
PRINTSiPR00454. ETSDOMAIN.
SMARTiSM00413. ETS. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
PROSITEiPS00345. ETS_DOMAIN_1. 1 hit.
PS00346. ETS_DOMAIN_2. 1 hit.
PS50061. ETS_DOMAIN_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSPIB_RAT
AccessioniPrimary (citable) accession number: Q5EBA3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: March 15, 2005
Last modified: September 7, 2016
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.