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Protein

ATP-dependent RNA helicase SUPV3L1, mitochondrial

Gene

Supv3l1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Major helicase player in mitochondrial RNA metabolism. Component of the mitochondrial degradosome (mtEXO) complex, that degrades 3' overhang double-stranded RNA with a 3'-to-5' directionality in an ATP-dependent manner. ATPase and ATP-dependent multisubstrate helicase, able to unwind double-stranded (ds) DNA and RNA, and RNA/DNA heteroduplexes in the 5'-to-3' direction. Plays a role in the RNA surveillance system in mitochondria; regulates the stability of mature mRNAs, the removal of aberrantly formed mRNAs and the rapid degradation of non coding processing intermediates. Also implicated in recombination and chromatin maintenance pathways. May protect cells from apoptosis. Associates with mitochondrial DNA.By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.By similarity

Cofactori

Mg2+By similarity, Mn2+By similarity

Enzyme regulationi

Helicase activity toward DNA substrate is inhibited by micromolar concentrations of 5,6-dichloro-1-(beta-D-ribofuranosyl)benzotriazole (DRBT) and 4,5,6,7-tetrabromobenzotriazole (TBBT). Helicase activity toward RNA substrate is inhibited by elevated concentrations of TBBT. Inhibited by some ring-expanded nucleoside analogs.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi207 – 214ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHelicase, Hydrolase
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent RNA helicase SUPV3L1, mitochondrial (EC:3.6.4.13By similarity)
Alternative name(s):
Suppressor of var1 3-like protein 1
Short name:
SUV3-like protein 1
Gene namesi
Name:Supv3l1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi1305565 Supv3l1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion, Mitochondrion nucleoid, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 40MitochondrionSequence analysisAdd BLAST40
ChainiPRO_000031054741 – 776ATP-dependent RNA helicase SUPV3L1, mitochondrialAdd BLAST736

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei99N6-acetyllysineBy similarity1
Modified residuei722PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ5EBA1
PRIDEiQ5EBA1

PTM databases

iPTMnetiQ5EBA1
PhosphoSitePlusiQ5EBA1

Interactioni

Subunit structurei

Homodimer; in free form. Component of the mitochondrial degradosome (mtEXO) complex which is a heteropentamer containing 2 copies of SUPV3L1 and 3 copies of PNPT1. Interacts with LAMTOR5/HBXIP, WRN and BLM.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000000441

Structurei

3D structure databases

ProteinModelPortaliQ5EBA1
SMRiQ5EBA1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini194 – 334Helicase ATP-bindingAdd BLAST141
Domaini353 – 521Helicase C-terminalPROSITE-ProRule annotationAdd BLAST169

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni650 – 776Interaction with LAMTOR5, important for protein stabilityBy similarityAdd BLAST127

Sequence similaritiesi

Belongs to the helicase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0953 Eukaryota
ENOG410XSEY LUCA
HOGENOMiHOG000175283
HOVERGENiHBG108522
InParanoidiQ5EBA1
KOiK17675
PhylomeDBiQ5EBA1

Family and domain databases

CDDicd00079 HELICc, 1 hit
InterProiView protein in InterPro
IPR001650 Helicase_C
IPR027417 P-loop_NTPase
IPR022192 SUV3_C
PfamiView protein in Pfam
PF00271 Helicase_C, 1 hit
PF12513 SUV3_C, 1 hit
SMARTiView protein in SMART
SM00490 HELICc, 1 hit
SUPFAMiSSF52540 SSF52540, 2 hits
PROSITEiView protein in PROSITE
PS51194 HELICASE_CTER, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5EBA1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLPRCALLW ARLPAGRGAG PRAAPCSALR ALVGSFPGAS GRVPCLAASS
60 70 80 90 100
SASGGSKAPN TSLFVPLTVK PQGPSADGDV GAELTRPLDK NEVKKILDKF
110 120 130 140 150
YKRQEIQKLS ADYGLDARLF HQAFISFRNY IMQSHSLDVD IHIVLNDICF
160 170 180 190 200
SAAHVDDLFP FFLRHAKQIF PVLECKDDLR QISDLRRPPN WYPEARAIQR
210 220 230 240 250
KIIFHSGPTN SGKTYHAIQR YLSATSGVYC GPLKLLAHEI FEKSNAAGVP
260 270 280 290 300
CDLVTGEERL TVEPEGKQAT HVSCTVEMCN VATPYEVAVI DEIQMIRDPA
310 320 330 340 350
RGWAWTRALL GLCAEEVHLC GESAAIDLVT ELLYTTGEEV EVQKYERLTP
360 370 380 390 400
ISVLDRALES LDNLRPGDCI VCFSKNDIYS VSRQIEIRGL ESAVIYGSLP
410 420 430 440 450
PGTKLAQARK FNDPNDPCKI LVATDAIGMG LNLSIRRIIF YSLIKPSINE
460 470 480 490 500
KGEKELEPIT TSQALQIAGR AGRFSSHFKE GEVTTMHRDD LALLKEILNR
510 520 530 540 550
PVDPIQAAGL HPTAEQIEMF AYHLPETTLS NLIDIFVDFA QVDGQYFVCN
560 570 580 590 600
MDDFKFSAEL IQHIPLSLRV RYVFCTAPIN KKQPFVCSSL LQFARQYSRN
610 620 630 640 650
EPLTFAWLRR YIKWPLLPPK NIKDLMDLEA VHDVFDLYLW LSYRFIDMFP
660 670 680 690 700
DSSFVRSLQK ELDVIIQEGV HNITKLIKIS ESHKLLNLEP SGSQSRLPGA
710 720 730 740 750
SKSPARRTRG TKTGNKAAEP PSPSDKELPL ASRLVQQGLL TADMLKQLQK
760 770
EWLTQRPEQG KEKVGTRRKK KDPNSD
Length:776
Mass (Da):86,706
Last modified:March 15, 2005 - v1
Checksum:iE2C263973DCC0396
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC089883 mRNA Translation: AAH89883.1
RefSeqiNP_001012480.1, NM_001012462.1
UniGeneiRn.19830

Genome annotation databases

GeneIDi294385
KEGGirno:294385
UCSCiRGD:1305565 rat

Similar proteinsi

Entry informationi

Entry nameiSUV3_RAT
AccessioniPrimary (citable) accession number: Q5EBA1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: March 15, 2005
Last modified: May 23, 2018
This is version 90 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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