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Reviewed, UniProtKB/Swiss-Prot Q5EB81 (NB5R1_RAT)

Last modified October 13, 2009. Version 37. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    NADH-cytochrome b5 reductase 1
      Short name=b5R.1
    EC=1.6.2.2
Gene names
Name: Cyb5r1
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

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Protein attributes

Sequence length305 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Function

NADH-cytochrome b5 reductases are involved in desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction By similarity.

Catalytic activity

NADH + 2 ferricytochrome b5 = NAD+ + H+ + 2 ferrocytochrome b5.

Cofactor

FAD By similarity.

Subcellular location

Membrane; Single-pass membrane protein Potential.

Sequence similarities

Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.

Contains 1 FAD-binding FR-type domain.

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Ontologies

Keywords
   Biological processLipid synthesis
Steroid biosynthesis
Sterol biosynthesis
   Cellular componentMembrane
   DomainTransmembrane
   LigandFAD
Flavoprotein
NAD
   Molecular functionOxidoreductase
Gene Ontology (GO)
   Biological processoxidation reduction

Inferred from electronic annotation. Source: UniProtKB-KW

sterol biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentintegral to membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functioncytochrome-b5 reductase activity

Inferred from electronic annotation. Source: EC

electron carrier activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 305305NADH-cytochrome b5 reductase 1
PRO_0000287547

Regions

Transmembrane8 – 2821 Potential
Domain44 – 156113FAD-binding FR-type
Nucleotide binding136 – 16631FAD By similarity
Nucleotide binding175 – 21036FAD By similarity

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Sequences

Sequence LengthMass (Da)Tools
Q5EB81-1 [UniParc].

Last modified March 15, 2005. Version 1.
Checksum: 759E91678EEB3D30

FASTA30534,223
        10         20         30         40         50         60 
MGIQPSPVLL ASLGVGLFTL FGLALGTYLV RRSRRPQVTL QDPDEKYLLR LLDKTTVSHN 

        70         80         90        100        110        120 
TRRFRFALPT AHHILGLPVG KHVYLSARID GSQVIRPYTP VTSDEDQGYV DLVIKVYLKG 

       130        140        150        160        170        180 
VHPKFSEGGK MSQYLDSLKI GDVVEFRGPS GLLSYAGKGN FNIQPNKKSP PELRVAKKLG 

       190        200        210        220        230        240 
MIAGGTGITP MLQLIRAILK VPEDPTQCFL LFANQTEKDI ILREDLEELQ AQYPIRFKLW 

       250        260        270        280        290        300 
FTLDYPPEDW TYSKGFVTAD MIQEHLPAPA EDVLLLLCGP PPMVQLACHP NLDKLGYSQK 


MRFTY

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References

[1]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
+Additional computationally mapped references.

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Cross-references

Sequence databases

BC089945 mRNA. Translation: AAH89945.1.
IPIIPI00371971.
RefSeqNP_001013144.1.
UniGeneRn.100496

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRINGQ5EB81.

Genome annotation databases

EnsemblENSRNOT00000005485; ENSRNOP00000005485; ENSRNOG00000003973; Rattus norvegicus. [Genome view]
ENSRNOT00000060669; ENSRNOP00000057400; ENSRNOG00000003973; Rattus norvegicus. [Genome view]
GeneID304805.
KEGGrno:304805.
UCSCNM_001013126. rat.

Organism-specific databases

CTD304805.
RGD1306084. Cyb5r1.

Phylogenomic databases

HOVERGENQ5EB81.

Enzyme and pathway databases

BRENDA1.6.2.2. 248.

Gene expression databases

ArrayExpressQ5EB81.
GenevestigatorQ5EB81.

Family and domain databases

InterProIPR017927. Fd_Rdtase_FAD-bd.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR001834. NADH-Cyt_B5_reductase.
IPR008333. OxRdtase_FAD-bd.
IPR001433. OxRdtase_FAD/NAD_bd.
[Graphical view]
PfamPF00970. FAD_binding_6. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PRINTSPR00406. CYTB5RDTASE.
PR00371. FPNCR.
PROSITEPS51384. FAD_FR. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio653648.

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Entry information

Entry nameNB5R1_RAT
AccessionPrimary (citable) accession number: Q5EB81
Entry history
Integrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: March 15, 2005
Last modified: October 13, 2009
This is version 37 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents