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Protein

Mediator of RNA polymerase II transcription subunit 23

Gene

Med23

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors. Also required for transcriptional activation subsequent to the assembly of the pre-initiation complex. Required for transcriptional activation by adenovirus E1A protein. Required for ELK1-dependent transcriptional activation in response to activated Ras signaling (By similarity).By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 23
Alternative name(s):
Cofactor required for Sp1 transcriptional activation subunit 3
Short name:
CRSP complex subunit 3
Mediator complex subunit 23
Gene namesi
Name:Med23
Synonyms:Crsp3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi1307671. Med23.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: UniProtKB-SubCell
  • ubiquitin ligase complex Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003059321 – 1367Mediator of RNA polymerase II transcription subunit 23Add BLAST1367

Proteomic databases

PaxDbiQ5EB59.
PRIDEiQ5EB59.

PTM databases

iPTMnetiQ5EB59.
PhosphoSitePlusiQ5EB59.

Expressioni

Gene expression databases

BgeeiENSRNOG00000013422.
GenevisibleiQ5EB59. RN.

Interactioni

Subunit structurei

Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Interacts with CDK8, CEBPB, CTNNB1, ELK1 and GLI3. Interacts with the adenovirus E1A protein (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000036610.

Family & Domainsi

Sequence similaritiesi

Belongs to the Mediator complex subunit 23 family.Curated

Phylogenomic databases

eggNOGiKOG1883. Eukaryota.
ENOG410ZH5R. LUCA.
HOGENOMiHOG000043810.
HOVERGENiHBG051122.
InParanoidiQ5EB59.
OrthoDBiEOG091G0EPL.

Family and domain databases

InterProiIPR021629. Mediator_Med23.
[Graphical view]
PfamiPF11573. Med23. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5EB59-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
METQLQSIFE EVVKTEIIEE AFPGMFMDTP EDEKTKLISC LAAFRQFWNG
60 70 80 90 100
LSQESHEQCV QWIVKFIHGQ HSPKRISFLY DCLAMAVETG LLPPRMVCES
110 120 130 140 150
LINSDTLEWE RTQLWALTFK LVRKIIGGVD YKGVRDLLKV ILEKILTIPN
160 170 180 190 200
TVSSAVVQQL LAAREVIAYI LERNACLLPA YFAVTEIRKL YPEGKLPHWL
210 220 230 240 250
LGNLVSDFVD TFRPTARINS ICGRCSLLPV VNNSGAICNS WKLDPATLRF
260 270 280 290 300
PLKGLLPYDK DLFEPQTALL RYVLEQPYSR DMVCNMLGLN KQHKQRCPVL
310 320 330 340 350
EDQLVDLVVY AMERSETEEK FDDGGTSQLL WQHLSSQLIF FVLFQFASFP
360 370 380 390 400
HMVLSLHQKL AGRGLIKGRD HLMWVLLQFI SGSIQKNALA DFLPVMKLFD
410 420 430 440 450
LLYPEKECIP VPDINKPQST HAFAMTCIWI HLNRKAQNDN SKLQIPIPHS
460 470 480 490 500
LKLHHEFLQQ SLRNKSLQMN DYKIALLCNA YSTNSECFTL PMGALVETIY
510 520 530 540 550
GNGIMRVPLP GTSCLASASV TPLPMNLLDS LTVHAKMSLI HSIATRVIKL
560 570 580 590 600
AHTKSSVALA PALVETYSRL LVYMEIESLG IKGFISQLLP TVFKSHAWGI
610 620 630 640 650
LHTLLEMFSH RMHHIQPHYR VQLLSHLHTL AAVAQTNQNQ LHLCVESTAL
660 670 680 690 700
RLITALGSSE VQPQFTRFLN DPKTVLSAES EELNRALILT LARATHVTDF
710 720 730 740 750
FTGSDSIQGT WCKDILQTIM TFTPHNWASH TLSCFPAPLQ AFFKQNNVPQ
760 770 780 790 800
ESRFNLKKNV EEEYRKWKSM TDENEIITQF SMQGFPPLFL CLLWKMLLET
810 820 830 840 850
DHISQIGYKV LERIGARALV AHVRTFADFL VYEFSTSAGG QQLNKCIEIL
860 870 880 890 900
NDMVWKYNIV TLDRLILCLA MRSHEGNEAQ VCYFIIQLLL LKPNDFRNRV
910 920 930 940 950
SDFVKENSPE HWLQSDWHTK HMSYHKKYPE KLYFEGLAEQ VDPPVPIQSP
960 970 980 990 1000
YLPIYFGNVC LRFLPVFDIV IHRFLELLPV SKSLETLLDH LGGLYKFHDR
1010 1020 1030 1040 1050
PVTYLYNTLH YYEMCLRNRD HLKRKLVHAI IGSLKDNRPQ GWCLSDTYLK
1060 1070 1080 1090 1100
HAMNAREDNP WVPEDSYYCK LIGRLVDTMA GKSPGPFPNC DWRFNEFPNP
1110 1120 1130 1140 1150
AAHALHVTCV ELMALAVPGK DVGNALLNVV LKSQPLVPRE NITAWMNAIG
1160 1170 1180 1190 1200
LIITALPEPY WIVLHDRIVS VISSSSLTSE TEWVGYPFRL FDFTACHQSY
1210 1220 1230 1240 1250
SEMSCSYTLA LAHAVWHHSS IGQLSLIPKF LTEVLLPVVK TEFQLLYVYH
1260 1270 1280 1290 1300
LVGPFLQRFQ QERTRCMIEI GVAFYDMLLN VDQCSTHLNY MDPICDFLYH
1310 1320 1330 1340 1350
MKYMFTGDSV KEQVEKIICN LKPALKLRLR FITHISKMEP AVPPQALSSG
1360
SPAPQANQVP TALPVTQ
Length:1,367
Mass (Da):156,232
Last modified:October 2, 2007 - v2
Checksum:i5C067F7FD80F3132
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AABR03000314 Genomic DNA. No translation available.
BC090022 mRNA. Translation: AAH90022.1.
UniGeneiRn.6604.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AABR03000314 Genomic DNA. No translation available.
BC090022 mRNA. Translation: AAH90022.1.
UniGeneiRn.6604.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000036610.

PTM databases

iPTMnetiQ5EB59.
PhosphoSitePlusiQ5EB59.

Proteomic databases

PaxDbiQ5EB59.
PRIDEiQ5EB59.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

RGDi1307671. Med23.

Phylogenomic databases

eggNOGiKOG1883. Eukaryota.
ENOG410ZH5R. LUCA.
HOGENOMiHOG000043810.
HOVERGENiHBG051122.
InParanoidiQ5EB59.
OrthoDBiEOG091G0EPL.

Miscellaneous databases

PROiQ5EB59.

Gene expression databases

BgeeiENSRNOG00000013422.
GenevisibleiQ5EB59. RN.

Family and domain databases

InterProiIPR021629. Mediator_Med23.
[Graphical view]
PfamiPF11573. Med23. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMED23_RAT
AccessioniPrimary (citable) accession number: Q5EB59
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: October 2, 2007
Last modified: November 2, 2016
This is version 65 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.