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Protein

Mesoderm-specific transcript homolog protein

Gene

MEST

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

GO - Molecular functioni

  1. hydrolase activity Source: UniProtKB-KW

GO - Biological processi

  1. mesoderm development Source: ProtInc
  2. regulation of lipid storage Source: Ensembl
  3. response to retinoic acid Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Protein family/group databases

MEROPSiS33.972.

Names & Taxonomyi

Protein namesi
Recommended name:
Mesoderm-specific transcript homolog protein (EC:3.-.-.-)
Alternative name(s):
Paternally-expressed gene 1 protein
Gene namesi
Name:MEST
Synonyms:PEG1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 7

Organism-specific databases

HGNCiHGNC:7028. MEST.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei13 – 3321HelicalSequence AnalysisAdd
BLAST
Transmembranei63 – 8321HelicalSequence AnalysisAdd
BLAST
Transmembranei266 – 28621HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB
  2. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  3. extracellular vesicular exosome Source: UniProtKB
  4. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA30762.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 335335Mesoderm-specific transcript homolog proteinPRO_0000284418Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi163 – 1631N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ5EB52.
PaxDbiQ5EB52.
PRIDEiQ5EB52.

PTM databases

PhosphoSiteiQ5EB52.

Expressioni

Tissue specificityi

Highly expressed in hydatidiform moles, but barely expressed in dermoid cysts. Biallelic expression is detected in blood lymphocytes. Seems to imprinted in an isoform-specific manner rather than in a tissue-specific manner in lymphocytes. Isoform 1 is expressed only from the paternal allele. Isoform 2 is expressed from both the paternal allele and the maternal allele.2 Publications

Developmental stagei

Monoallelic expression of paternally derived allele was observed in all fetal tissues examined, including brain, skeletal muscle, kidney, adrenal, tongue, heart, skin and placenta. In 75-day fetus, expressed in the amnion, brain, heart, lung, stomach, gut, adrenal, kidney, muscle and liver.3 Publications

Gene expression databases

BgeeiQ5EB52.
CleanExiHS_MEST.
ExpressionAtlasiQ5EB52. baseline and differential.
GenevestigatoriQ5EB52.

Organism-specific databases

HPAiHPA005623.

Interactioni

Protein-protein interaction databases

BioGridi110390. 18 interactions.
IntActiQ5EB52. 14 interactions.
MINTiMINT-8247567.
STRINGi9606.ENSP00000223215.

Structurei

3D structure databases

ProteinModelPortaliQ5EB52.
SMRiQ5EB52. Positions 52-333.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi98 – 1036RVIALD

Sequence similaritiesi

Belongs to the AB hydrolase superfamily.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0596.
GeneTreeiENSGT00510000047602.
HOGENOMiHOG000049677.
HOVERGENiHBG052816.
InParanoidiQ5EB52.
OMAiCDMLGFG.
PhylomeDBiQ5EB52.
TreeFamiTF329307.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR000639. Epox_hydrolase-like.
[Graphical view]
PRINTSiPR00412. EPOXHYDRLASE.
SUPFAMiSSF53474. SSF53474. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q5EB52-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVRRDRLRRM REWWVQVGLL AVPLLAAYLH IPPPQLSPAL HSWKSSGKFF
60 70 80 90 100
TYKGLRIFYQ DSVGVVGSPE IVVLLHGFPT SSYDWYKIWE GLTLRFHRVI
110 120 130 140 150
ALDFLGFGFS DKPRPHHYSI FEQASIVEAL LRHLGLQNRR INLLSHDYGD
160 170 180 190 200
IVAQELLYRY KQNRSGRLTI KSLCLSNGGI FPETHRPLLL QKLLKDGGVL
210 220 230 240 250
SPILTRLMNF FVFSRGLTPV FGPYTRPSES ELWDMWAGIR NNDGNLVIDS
260 270 280 290 300
LLQYINQRKK FRRRWVGALA SVTIPIHFIY GPLDPVNPYP EFLELYRKTL
310 320 330
PRSTVSILDD HISHYPQLED PMGFLNAYMG FINSF
Length:335
Mass (Da):38,830
Last modified:April 17, 2007 - v2
Checksum:iD2EC0CD6BEC9079A
GO
Isoform 2 (identifier: Q5EB52-2) [UniParc]FASTAAdd to basket

Also known as: Isoform b

The sequence of this isoform differs from the canonical sequence as follows:
     1-9: Missing.

Show »
Length:326
Mass (Da):37,591
Checksum:i3B524B416A48A292
GO
Isoform 3 (identifier: Q5EB52-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-9: Missing.
     218-251: Missing.

Note: No experimental confirmation available.

Show »
Length:292
Mass (Da):33,757
Checksum:iE0F310C8D967DB49
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti90 – 901E → K in BAA11432 (PubMed:8884280).Curated
Sequence conflicti168 – 1681L → H in BAA11432 (PubMed:8884280).Curated
Sequence conflicti222 – 2221G → W in BAA21757 (PubMed:9158153).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 99Missing in isoform 2 and isoform 3. 2 PublicationsVSP_024532
Alternative sequencei218 – 25134Missing in isoform 3. 1 PublicationVSP_024533Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D78611 mRNA. Translation: BAA11432.1.
Y11534 mRNA. Translation: CAA72297.1.
D87367 mRNA. Translation: BAA21757.1.
AB045582 Genomic DNA. Translation: BAC02716.1.
BT007056 mRNA. Translation: AAP35705.1.
CR457040 mRNA. Translation: CAG33321.1.
AK312688 mRNA. Translation: BAG35568.1.
CR627364 mRNA. Translation: CAH10465.1.
CH471070 Genomic DNA. Translation: EAW83766.1.
BC002413 mRNA. Translation: AAH02413.1.
BC090049 mRNA. Translation: AAH90049.1.
BC011908 mRNA. Translation: AAH11908.1.
BC014564 mRNA. Translation: AAH14564.1.
BC018695 mRNA. Translation: AAH18695.1.
CCDSiCCDS5822.1. [Q5EB52-1]
CCDS5823.1. [Q5EB52-2]
CCDS59081.1. [Q5EB52-3]
RefSeqiNP_001240829.1. NM_001253900.1.
NP_001240830.1. NM_001253901.1. [Q5EB52-3]
NP_001240831.1. NM_001253902.1. [Q5EB52-3]
NP_002393.2. NM_002402.3. [Q5EB52-1]
NP_803490.1. NM_177524.2. [Q5EB52-2]
NP_803491.1. NM_177525.2. [Q5EB52-2]
UniGeneiHs.270978.

Genome annotation databases

EnsembliENST00000223215; ENSP00000223215; ENSG00000106484. [Q5EB52-1]
ENST00000341441; ENSP00000342749; ENSG00000106484. [Q5EB52-2]
ENST00000378576; ENSP00000367839; ENSG00000106484. [Q5EB52-3]
ENST00000393187; ENSP00000376884; ENSG00000106484. [Q5EB52-2]
ENST00000416162; ENSP00000408933; ENSG00000106484. [Q5EB52-3]
GeneIDi4232.
KEGGihsa:4232.
UCSCiuc003vqc.3. human. [Q5EB52-1]
uc003vqd.3. human. [Q5EB52-3]

Polymorphism databases

DMDMi145566794.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D78611 mRNA. Translation: BAA11432.1.
Y11534 mRNA. Translation: CAA72297.1.
D87367 mRNA. Translation: BAA21757.1.
AB045582 Genomic DNA. Translation: BAC02716.1.
BT007056 mRNA. Translation: AAP35705.1.
CR457040 mRNA. Translation: CAG33321.1.
AK312688 mRNA. Translation: BAG35568.1.
CR627364 mRNA. Translation: CAH10465.1.
CH471070 Genomic DNA. Translation: EAW83766.1.
BC002413 mRNA. Translation: AAH02413.1.
BC090049 mRNA. Translation: AAH90049.1.
BC011908 mRNA. Translation: AAH11908.1.
BC014564 mRNA. Translation: AAH14564.1.
BC018695 mRNA. Translation: AAH18695.1.
CCDSiCCDS5822.1. [Q5EB52-1]
CCDS5823.1. [Q5EB52-2]
CCDS59081.1. [Q5EB52-3]
RefSeqiNP_001240829.1. NM_001253900.1.
NP_001240830.1. NM_001253901.1. [Q5EB52-3]
NP_001240831.1. NM_001253902.1. [Q5EB52-3]
NP_002393.2. NM_002402.3. [Q5EB52-1]
NP_803490.1. NM_177524.2. [Q5EB52-2]
NP_803491.1. NM_177525.2. [Q5EB52-2]
UniGeneiHs.270978.

3D structure databases

ProteinModelPortaliQ5EB52.
SMRiQ5EB52. Positions 52-333.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi110390. 18 interactions.
IntActiQ5EB52. 14 interactions.
MINTiMINT-8247567.
STRINGi9606.ENSP00000223215.

Protein family/group databases

MEROPSiS33.972.

PTM databases

PhosphoSiteiQ5EB52.

Polymorphism databases

DMDMi145566794.

Proteomic databases

MaxQBiQ5EB52.
PaxDbiQ5EB52.
PRIDEiQ5EB52.

Protocols and materials databases

DNASUi4232.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000223215; ENSP00000223215; ENSG00000106484. [Q5EB52-1]
ENST00000341441; ENSP00000342749; ENSG00000106484. [Q5EB52-2]
ENST00000378576; ENSP00000367839; ENSG00000106484. [Q5EB52-3]
ENST00000393187; ENSP00000376884; ENSG00000106484. [Q5EB52-2]
ENST00000416162; ENSP00000408933; ENSG00000106484. [Q5EB52-3]
GeneIDi4232.
KEGGihsa:4232.
UCSCiuc003vqc.3. human. [Q5EB52-1]
uc003vqd.3. human. [Q5EB52-3]

Organism-specific databases

CTDi4232.
GeneCardsiGC07P130126.
H-InvDBHIX0167823.
HGNCiHGNC:7028. MEST.
HPAiHPA005623.
MIMi601029. gene.
neXtProtiNX_Q5EB52.
PharmGKBiPA30762.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG0596.
GeneTreeiENSGT00510000047602.
HOGENOMiHOG000049677.
HOVERGENiHBG052816.
InParanoidiQ5EB52.
OMAiCDMLGFG.
PhylomeDBiQ5EB52.
TreeFamiTF329307.

Miscellaneous databases

ChiTaRSiMEST. human.
GeneWikiiMEST_(gene).
GenomeRNAii4232.
NextBioi16681.
PROiQ5EB52.
SOURCEiSearch...

Gene expression databases

BgeeiQ5EB52.
CleanExiHS_MEST.
ExpressionAtlasiQ5EB52. baseline and differential.
GenevestigatoriQ5EB52.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR000639. Epox_hydrolase-like.
[Graphical view]
PRINTSiPR00412. EPOXHYDRLASE.
SUPFAMiSSF53474. SSF53474. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genomic imprinting and chromosomal localization of the human MEST gene."
    Nishita Y., Yoshida I., Sado T., Takagi N.
    Genomics 36:539-542(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Tissue: Fetus.
  2. "Monoallelic expression of human PEG1/MEST is paralleled by parent-specific methylation in fetuses."
    Riesewijk A.M., Hu L., Schulz U., Tariverdian G., Hoglund P., Kere J., Ropers H.-H., Kalscheuer V.M.
    Genomics 42:236-244(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Tissue: Fetus.
  3. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), DEVELOPMENTAL STAGE.
    Tissue: Fetal kidney.
  4. "Screening for the mutations of the PEG1/MEST gene in Silver Russell Syndrome."
    Kobayashi S., Uemura H., Kohda T., Niikawa N., Yamada M., Yamasaki R., Kaneko-Ishino T., Ishino F.
    Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Tissue: Placenta.
  5. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
    Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
    Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  6. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  7. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  8. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Small intestine.
  9. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  10. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
    Tissue: Muscle and Testis.
  11. "Isoform-specific imprinting of the human PEG1/MEST gene."
    Kosaki K., Kosaki R., Craigen W.J., Matsuo N.
    Am. J. Hum. Genet. 66:309-312(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY (ISOFORMS 1 AND 2).

Entry informationi

Entry nameiMEST_HUMAN
AccessioniPrimary (citable) accession number: Q5EB52
Secondary accession number(s): B2R6S1
, O14973, O15007, Q6AI49, Q92571
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 17, 2007
Last sequence update: April 17, 2007
Last modified: January 7, 2015
This is version 93 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.