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Q5EB52

- MEST_HUMAN

UniProt

Q5EB52 - MEST_HUMAN

Protein

Mesoderm-specific transcript homolog protein

Gene

MEST

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 90 (01 Oct 2014)
      Sequence version 2 (17 Apr 2007)
      Previous versions | rss
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    Functioni

    GO - Molecular functioni

    1. hydrolase activity Source: UniProtKB-KW

    GO - Biological processi

    1. mesoderm development Source: ProtInc
    2. regulation of lipid storage Source: Ensembl
    3. response to retinoic acid Source: Ensembl

    Keywords - Molecular functioni

    Hydrolase

    Protein family/group databases

    MEROPSiS33.972.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mesoderm-specific transcript homolog protein (EC:3.-.-.-)
    Alternative name(s):
    Paternally-expressed gene 1 protein
    Gene namesi
    Name:MEST
    Synonyms:PEG1
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 7

    Organism-specific databases

    HGNCiHGNC:7028. MEST.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum Source: UniProtKB
    2. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    3. extracellular vesicular exosome Source: UniProt
    4. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA30762.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 335335Mesoderm-specific transcript homolog proteinPRO_0000284418Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi163 – 1631N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    MaxQBiQ5EB52.
    PaxDbiQ5EB52.
    PRIDEiQ5EB52.

    PTM databases

    PhosphoSiteiQ5EB52.

    Expressioni

    Tissue specificityi

    Highly expressed in hydatidiform moles, but barely expressed in dermoid cysts. Biallelic expression is detected in blood lymphocytes. Seems to imprinted in an isoform-specific manner rather than in a tissue-specific manner in lymphocytes. Isoform 1 is expressed only from the paternal allele. Isoform 2 is expressed from both the paternal allele and the maternal allele.2 Publications

    Developmental stagei

    Monoallelic expression of paternally derived allele was observed in all fetal tissues examined, including brain, skeletal muscle, kidney, adrenal, tongue, heart, skin and placenta. In 75-day fetus, expressed in the amnion, brain, heart, lung, stomach, gut, adrenal, kidney, muscle and liver.3 Publications

    Gene expression databases

    ArrayExpressiQ5EB52.
    BgeeiQ5EB52.
    CleanExiHS_MEST.
    GenevestigatoriQ5EB52.

    Organism-specific databases

    HPAiHPA005623.

    Interactioni

    Protein-protein interaction databases

    BioGridi110390. 18 interactions.
    IntActiQ5EB52. 14 interactions.
    MINTiMINT-8247567.
    STRINGi9606.ENSP00000223215.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5EB52.
    SMRiQ5EB52. Positions 48-333.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei13 – 3321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei63 – 8321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei266 – 28621HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi98 – 1036RVIALD

    Sequence similaritiesi

    Belongs to the AB hydrolase superfamily.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0596.
    HOGENOMiHOG000049677.
    HOVERGENiHBG052816.
    InParanoidiQ5EB52.
    OMAiSVSIPIH.
    PhylomeDBiQ5EB52.
    TreeFamiTF329307.

    Family and domain databases

    Gene3Di3.40.50.1820. 1 hit.
    InterProiIPR029058. AB_hydrolase.
    IPR000639. Epox_hydrolase-like.
    [Graphical view]
    PRINTSiPR00412. EPOXHYDRLASE.
    SUPFAMiSSF53474. SSF53474. 1 hit.

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q5EB52-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MVRRDRLRRM REWWVQVGLL AVPLLAAYLH IPPPQLSPAL HSWKSSGKFF    50
    TYKGLRIFYQ DSVGVVGSPE IVVLLHGFPT SSYDWYKIWE GLTLRFHRVI 100
    ALDFLGFGFS DKPRPHHYSI FEQASIVEAL LRHLGLQNRR INLLSHDYGD 150
    IVAQELLYRY KQNRSGRLTI KSLCLSNGGI FPETHRPLLL QKLLKDGGVL 200
    SPILTRLMNF FVFSRGLTPV FGPYTRPSES ELWDMWAGIR NNDGNLVIDS 250
    LLQYINQRKK FRRRWVGALA SVTIPIHFIY GPLDPVNPYP EFLELYRKTL 300
    PRSTVSILDD HISHYPQLED PMGFLNAYMG FINSF 335
    Length:335
    Mass (Da):38,830
    Last modified:April 17, 2007 - v2
    Checksum:iD2EC0CD6BEC9079A
    GO
    Isoform 2 (identifier: Q5EB52-2) [UniParc]FASTAAdd to Basket

    Also known as: Isoform b

    The sequence of this isoform differs from the canonical sequence as follows:
         1-9: Missing.

    Show »
    Length:326
    Mass (Da):37,591
    Checksum:i3B524B416A48A292
    GO
    Isoform 3 (identifier: Q5EB52-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-9: Missing.
         218-251: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:292
    Mass (Da):33,757
    Checksum:iE0F310C8D967DB49
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti90 – 901E → K in BAA11432. (PubMed:8884280)Curated
    Sequence conflicti168 – 1681L → H in BAA11432. (PubMed:8884280)Curated
    Sequence conflicti222 – 2221G → W in BAA21757. (PubMed:9158153)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 99Missing in isoform 2 and isoform 3. 2 PublicationsVSP_024532
    Alternative sequencei218 – 25134Missing in isoform 3. 1 PublicationVSP_024533Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D78611 mRNA. Translation: BAA11432.1.
    Y11534 mRNA. Translation: CAA72297.1.
    D87367 mRNA. Translation: BAA21757.1.
    AB045582 Genomic DNA. Translation: BAC02716.1.
    BT007056 mRNA. Translation: AAP35705.1.
    CR457040 mRNA. Translation: CAG33321.1.
    AK312688 mRNA. Translation: BAG35568.1.
    CR627364 mRNA. Translation: CAH10465.1.
    CH471070 Genomic DNA. Translation: EAW83766.1.
    BC002413 mRNA. Translation: AAH02413.1.
    BC090049 mRNA. Translation: AAH90049.1.
    BC011908 mRNA. Translation: AAH11908.1.
    BC014564 mRNA. Translation: AAH14564.1.
    BC018695 mRNA. Translation: AAH18695.1.
    CCDSiCCDS5822.1. [Q5EB52-1]
    CCDS5823.1. [Q5EB52-2]
    CCDS59081.1. [Q5EB52-3]
    RefSeqiNP_001240829.1. NM_001253900.1.
    NP_001240830.1. NM_001253901.1. [Q5EB52-3]
    NP_001240831.1. NM_001253902.1. [Q5EB52-3]
    NP_002393.2. NM_002402.3. [Q5EB52-1]
    NP_803490.1. NM_177524.2. [Q5EB52-2]
    NP_803491.1. NM_177525.2. [Q5EB52-2]
    UniGeneiHs.270978.

    Genome annotation databases

    EnsembliENST00000223215; ENSP00000223215; ENSG00000106484. [Q5EB52-1]
    ENST00000341441; ENSP00000342749; ENSG00000106484. [Q5EB52-2]
    ENST00000378576; ENSP00000367839; ENSG00000106484. [Q5EB52-3]
    ENST00000393187; ENSP00000376884; ENSG00000106484. [Q5EB52-2]
    ENST00000416162; ENSP00000408933; ENSG00000106484. [Q5EB52-3]
    GeneIDi4232.
    KEGGihsa:4232.
    UCSCiuc003vqc.3. human. [Q5EB52-1]
    uc003vqd.3. human. [Q5EB52-3]

    Polymorphism databases

    DMDMi145566794.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D78611 mRNA. Translation: BAA11432.1 .
    Y11534 mRNA. Translation: CAA72297.1 .
    D87367 mRNA. Translation: BAA21757.1 .
    AB045582 Genomic DNA. Translation: BAC02716.1 .
    BT007056 mRNA. Translation: AAP35705.1 .
    CR457040 mRNA. Translation: CAG33321.1 .
    AK312688 mRNA. Translation: BAG35568.1 .
    CR627364 mRNA. Translation: CAH10465.1 .
    CH471070 Genomic DNA. Translation: EAW83766.1 .
    BC002413 mRNA. Translation: AAH02413.1 .
    BC090049 mRNA. Translation: AAH90049.1 .
    BC011908 mRNA. Translation: AAH11908.1 .
    BC014564 mRNA. Translation: AAH14564.1 .
    BC018695 mRNA. Translation: AAH18695.1 .
    CCDSi CCDS5822.1. [Q5EB52-1 ]
    CCDS5823.1. [Q5EB52-2 ]
    CCDS59081.1. [Q5EB52-3 ]
    RefSeqi NP_001240829.1. NM_001253900.1.
    NP_001240830.1. NM_001253901.1. [Q5EB52-3 ]
    NP_001240831.1. NM_001253902.1. [Q5EB52-3 ]
    NP_002393.2. NM_002402.3. [Q5EB52-1 ]
    NP_803490.1. NM_177524.2. [Q5EB52-2 ]
    NP_803491.1. NM_177525.2. [Q5EB52-2 ]
    UniGenei Hs.270978.

    3D structure databases

    ProteinModelPortali Q5EB52.
    SMRi Q5EB52. Positions 48-333.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 110390. 18 interactions.
    IntActi Q5EB52. 14 interactions.
    MINTi MINT-8247567.
    STRINGi 9606.ENSP00000223215.

    Protein family/group databases

    MEROPSi S33.972.

    PTM databases

    PhosphoSitei Q5EB52.

    Polymorphism databases

    DMDMi 145566794.

    Proteomic databases

    MaxQBi Q5EB52.
    PaxDbi Q5EB52.
    PRIDEi Q5EB52.

    Protocols and materials databases

    DNASUi 4232.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000223215 ; ENSP00000223215 ; ENSG00000106484 . [Q5EB52-1 ]
    ENST00000341441 ; ENSP00000342749 ; ENSG00000106484 . [Q5EB52-2 ]
    ENST00000378576 ; ENSP00000367839 ; ENSG00000106484 . [Q5EB52-3 ]
    ENST00000393187 ; ENSP00000376884 ; ENSG00000106484 . [Q5EB52-2 ]
    ENST00000416162 ; ENSP00000408933 ; ENSG00000106484 . [Q5EB52-3 ]
    GeneIDi 4232.
    KEGGi hsa:4232.
    UCSCi uc003vqc.3. human. [Q5EB52-1 ]
    uc003vqd.3. human. [Q5EB52-3 ]

    Organism-specific databases

    CTDi 4232.
    GeneCardsi GC07P130126.
    H-InvDB HIX0167823.
    HGNCi HGNC:7028. MEST.
    HPAi HPA005623.
    MIMi 601029. gene.
    neXtProti NX_Q5EB52.
    PharmGKBi PA30762.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG0596.
    HOGENOMi HOG000049677.
    HOVERGENi HBG052816.
    InParanoidi Q5EB52.
    OMAi SVSIPIH.
    PhylomeDBi Q5EB52.
    TreeFami TF329307.

    Miscellaneous databases

    ChiTaRSi MEST. human.
    GeneWikii MEST_(gene).
    GenomeRNAii 4232.
    NextBioi 16681.
    PROi Q5EB52.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q5EB52.
    Bgeei Q5EB52.
    CleanExi HS_MEST.
    Genevestigatori Q5EB52.

    Family and domain databases

    Gene3Di 3.40.50.1820. 1 hit.
    InterProi IPR029058. AB_hydrolase.
    IPR000639. Epox_hydrolase-like.
    [Graphical view ]
    PRINTSi PR00412. EPOXHYDRLASE.
    SUPFAMi SSF53474. SSF53474. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Genomic imprinting and chromosomal localization of the human MEST gene."
      Nishita Y., Yoshida I., Sado T., Takagi N.
      Genomics 36:539-542(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
      Tissue: Fetus.
    2. "Monoallelic expression of human PEG1/MEST is paralleled by parent-specific methylation in fetuses."
      Riesewijk A.M., Hu L., Schulz U., Tariverdian G., Hoglund P., Kere J., Ropers H.-H., Kalscheuer V.M.
      Genomics 42:236-244(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
      Tissue: Fetus.
    3. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), DEVELOPMENTAL STAGE.
      Tissue: Fetal kidney.
    4. "Screening for the mutations of the PEG1/MEST gene in Silver Russell Syndrome."
      Kobayashi S., Uemura H., Kohda T., Niikawa N., Yamada M., Yamasaki R., Kaneko-Ishino T., Ishino F.
      Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Tissue: Placenta.
    5. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
      Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
      Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    6. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
      Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
      Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    7. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    8. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Small intestine.
    9. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    10. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
      Tissue: Muscle and Testis.
    11. "Isoform-specific imprinting of the human PEG1/MEST gene."
      Kosaki K., Kosaki R., Craigen W.J., Matsuo N.
      Am. J. Hum. Genet. 66:309-312(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY (ISOFORMS 1 AND 2).

    Entry informationi

    Entry nameiMEST_HUMAN
    AccessioniPrimary (citable) accession number: Q5EB52
    Secondary accession number(s): B2R6S1
    , O14973, O15007, Q6AI49, Q92571
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 17, 2007
    Last sequence update: April 17, 2007
    Last modified: October 1, 2014
    This is version 90 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 7
      Human chromosome 7: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3