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Q5EB52 (MEST_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mesoderm-specific transcript homolog protein

EC=3.-.-.-
Alternative name(s):
Paternally-expressed gene 1 protein
Gene names
Name:MEST
Synonyms:PEG1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length335 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.

Tissue specificity

Highly expressed in hydatidiform moles, but barely expressed in dermoid cysts. Biallelic expression is detected in blood lymphocytes. Seems to imprinted in an isoform-specific manner rather than in a tissue-specific manner in lymphocytes. Isoform 1 is expressed only from the paternal allele. Isoform 2 is expressed from both the paternal allele and the maternal allele. Ref.1 Ref.2 Ref.11

Developmental stage

Monoallelic expression of paternally derived allele was observed in all fetal tissues examined, including brain, skeletal muscle, kidney, adrenal, tongue, heart, skin and placenta. In 75-day fetus, expressed in the amnion, brain, heart, lung, stomach, gut, adrenal, kidney, muscle and liver. Ref.1 Ref.2 Ref.3

Sequence similarities

Belongs to the AB hydrolase superfamily.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q5EB52-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q5EB52-2)

Also known as: Isoform b;

The sequence of this isoform differs from the canonical sequence as follows:
     1-9: Missing.
Isoform 3 (identifier: Q5EB52-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-9: Missing.
     218-251: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 335335Mesoderm-specific transcript homolog protein
PRO_0000284418

Regions

Transmembrane13 – 3321Helical; Potential
Transmembrane63 – 8321Helical; Potential
Transmembrane266 – 28621Helical; Potential
Motif98 – 1036RVIALD

Amino acid modifications

Glycosylation1631N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence1 – 99Missing in isoform 2 and isoform 3.
VSP_024532
Alternative sequence218 – 25134Missing in isoform 3.
VSP_024533

Experimental info

Sequence conflict901E → K in BAA11432. Ref.1
Sequence conflict1681L → H in BAA11432. Ref.1
Sequence conflict2221G → W in BAA21757. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified April 17, 2007. Version 2.
Checksum: D2EC0CD6BEC9079A

FASTA33538,830
        10         20         30         40         50         60 
MVRRDRLRRM REWWVQVGLL AVPLLAAYLH IPPPQLSPAL HSWKSSGKFF TYKGLRIFYQ 

        70         80         90        100        110        120 
DSVGVVGSPE IVVLLHGFPT SSYDWYKIWE GLTLRFHRVI ALDFLGFGFS DKPRPHHYSI 

       130        140        150        160        170        180 
FEQASIVEAL LRHLGLQNRR INLLSHDYGD IVAQELLYRY KQNRSGRLTI KSLCLSNGGI 

       190        200        210        220        230        240 
FPETHRPLLL QKLLKDGGVL SPILTRLMNF FVFSRGLTPV FGPYTRPSES ELWDMWAGIR 

       250        260        270        280        290        300 
NNDGNLVIDS LLQYINQRKK FRRRWVGALA SVTIPIHFIY GPLDPVNPYP EFLELYRKTL 

       310        320        330 
PRSTVSILDD HISHYPQLED PMGFLNAYMG FINSF 

« Hide

Isoform 2 (Isoform b) [UniParc].

Checksum: 3B524B416A48A292
Show »

FASTA32637,591
Isoform 3 [UniParc].

Checksum: E0F310C8D967DB49
Show »

FASTA29233,757

References

« Hide 'large scale' references
[1]"Genomic imprinting and chromosomal localization of the human MEST gene."
Nishita Y., Yoshida I., Sado T., Takagi N.
Genomics 36:539-542(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
Tissue: Fetus.
[2]"Monoallelic expression of human PEG1/MEST is paralleled by parent-specific methylation in fetuses."
Riesewijk A.M., Hu L., Schulz U., Tariverdian G., Hoglund P., Kere J., Ropers H.-H., Kalscheuer V.M.
Genomics 42:236-244(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
Tissue: Fetus.
[3]"Human PEG1/MEST, an imprinted gene on chromosome 7."
Kobayashi S., Kohda T., Miyoshi N., Kuroiwa Y., Aisaka K., Tsutsumi O., Kaneko-Ishino T., Ishino F.
Hum. Mol. Genet. 6:781-786(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), DEVELOPMENTAL STAGE.
Tissue: Fetal kidney.
[4]"Screening for the mutations of the PEG1/MEST gene in Silver Russell Syndrome."
Kobayashi S., Uemura H., Kohda T., Niikawa N., Yamada M., Yamasaki R., Kaneko-Ishino T., Ishino F.
Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Tissue: Placenta.
[5]"Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[6]"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[7]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[8]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Small intestine.
[9]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[10]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
Tissue: Muscle and Testis.
[11]"Isoform-specific imprinting of the human PEG1/MEST gene."
Kosaki K., Kosaki R., Craigen W.J., Matsuo N.
Am. J. Hum. Genet. 66:309-312(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY (ISOFORMS 1 AND 2).
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D78611 mRNA. Translation: BAA11432.1.
Y11534 mRNA. Translation: CAA72297.1.
D87367 mRNA. Translation: BAA21757.1.
AB045582 Genomic DNA. Translation: BAC02716.1.
BT007056 mRNA. Translation: AAP35705.1.
CR457040 mRNA. Translation: CAG33321.1.
AK312688 mRNA. Translation: BAG35568.1.
CR627364 mRNA. Translation: CAH10465.1.
CH471070 Genomic DNA. Translation: EAW83766.1.
BC002413 mRNA. Translation: AAH02413.1.
BC090049 mRNA. Translation: AAH90049.1.
BC011908 mRNA. Translation: AAH11908.1.
BC014564 mRNA. Translation: AAH14564.1.
BC018695 mRNA. Translation: AAH18695.1.
RefSeqNP_001240829.1. NM_001253900.1.
NP_001240830.1. NM_001253901.1.
NP_001240831.1. NM_001253902.1.
NP_002393.2. NM_002402.3.
NP_803490.1. NM_177524.2.
NP_803491.1. NM_177525.2.
UniGeneHs.270978.

3D structure databases

ProteinModelPortalQ5EB52.
SMRQ5EB52. Positions 48-189.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid110390. 18 interactions.
IntActQ5EB52. 14 interactions.
MINTMINT-8247567.
STRING9606.ENSP00000223215.

Protein family/group databases

MEROPSS33.972.

PTM databases

PhosphoSiteQ5EB52.

Polymorphism databases

DMDM145566794.

Proteomic databases

PaxDbQ5EB52.
PRIDEQ5EB52.

Protocols and materials databases

DNASU4232.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000223215; ENSP00000223215; ENSG00000106484. [Q5EB52-1]
ENST00000341441; ENSP00000342749; ENSG00000106484. [Q5EB52-2]
ENST00000378576; ENSP00000367839; ENSG00000106484. [Q5EB52-3]
ENST00000393187; ENSP00000376884; ENSG00000106484. [Q5EB52-2]
ENST00000416162; ENSP00000408933; ENSG00000106484. [Q5EB52-3]
ENST00000578652; ENSP00000462286; ENSG00000264872. [Q5EB52-3]
ENST00000580802; ENSP00000463277; ENSG00000264872. [Q5EB52-1]
ENST00000583792; ENSP00000464161; ENSG00000264872. [Q5EB52-3]
ENST00000585116; ENSP00000463883; ENSG00000264872. [Q5EB52-2]
GeneID4232.
KEGGhsa:4232.
UCSCuc003vqc.3. human. [Q5EB52-1]
uc003vqd.3. human. [Q5EB52-3]

Organism-specific databases

CTD4232.
GeneCardsGC07P130126.
H-InvDBHIX0167823.
HGNCHGNC:7028. MEST.
HPAHPA005623.
MIM601029. gene.
neXtProtNX_Q5EB52.
PharmGKBPA30762.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0596.
HOGENOMHOG000049677.
HOVERGENHBG052816.
InParanoidQ5EB52.
OMACDMLGFG.
PhylomeDBQ5EB52.
TreeFamTF329307.

Gene expression databases

ArrayExpressQ5EB52.
BgeeQ5EB52.
CleanExHS_MEST.
GenevestigatorQ5EB52.

Family and domain databases

InterProIPR000639. Epox_hydrolase-like.
[Graphical view]
PRINTSPR00412. EPOXHYDRLASE.
ProtoNetSearch...

Other

ChiTaRSMEST. human.
GeneWikiMEST_(gene).
GenomeRNAi4232.
NextBio16681.
PROQ5EB52.
SOURCESearch...

Entry information

Entry nameMEST_HUMAN
AccessionPrimary (citable) accession number: Q5EB52
Secondary accession number(s): B2R6S1 expand/collapse secondary AC list , O14973, O15007, Q6AI49, Q92571
Entry history
Integrated into UniProtKB/Swiss-Prot: April 17, 2007
Last sequence update: April 17, 2007
Last modified: April 16, 2014
This is version 86 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 7

Human chromosome 7: entries, gene names and cross-references to MIM