Q5EAK6 (ATM_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein kinase ATM
EC=2.7.11.1

Alternative name(s):
Telomere fusion protein

Gene names

Name:	tefu
Synonyms:	atm
ORF Names:	CG6535

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	2767 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Serine/threonine-protein kinase which recognizes the substrate consensus sequence [ST]-Q. Required to suppress spontaneous apoptosis of proliferating cells during development, and for their proper differentiation. Required for female fertility. Protects telomeres from fusion, maybe by recruiting or maintaining chromatin-modifying complexes such as Su(var)205/HP1. May activate checkpoint signaling in response to DNA double-stranded breaks induced by low-dose ionizing radiation. May phosphorylate histone H2AV. Ref.5 Ref.6 Ref.7 Ref.8 Ref.9 Ref.10
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subcellular location	Nucleus Probable. Chromosome › telomere.
Developmental stage	Expressed at low levels throughout development and at much higher levels in adult females compared to males. Ref.7
Disruption phenotype	Flies die shortly before or after eclosion with a rough eye phenotype, misshapen wings, and missing or abnormal bristles. Ref.8
Sequence similarities	Belongs to the PI3/PI4-kinase family. ATM subfamily. Contains 1 FAT domain. Contains 1 FATC domain. Contains 1 PI3K/PI4K domain.
Sequence caution	The sequence AAM29197.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Cell cycle DNA damage DNA repair
Cellular component	Chromosome Nucleus Telomere
Ligand	ATP-binding Nucleotide-binding
Molecular function	Chromatin regulator Developmental protein Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW chromatin modification Inferred from electronic annotation. Source: UniProtKB-KW chromatin silencing at telomere Inferred from mutant phenotype Ref.8. Source: FlyBase mitotic cell cycle G2/M transition DNA damage checkpoint Inferred from genetic interaction. Source: FlyBase multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW photoreceptor cell maintenance Inferred from mutant phenotype. Source: FlyBase telomere capping Inferred from mutant phenotype. Source: FlyBase
Cellular component	chromosome, telomeric region Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 2767	2767	Serine/threonine-protein kinase ATM		PRO_0000225627
Regions
	Domain	1649 – 2317	669	FAT
	Domain	2443 – 2696	254	PI3K/PI4K
	Domain	2735 – 2767	33	FATC
Experimental info
	Sequence conflict	2733	1	T → P in AAM29197. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q5EAK6 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: F354E55FA1B35570	FASTA	2,767	317,991
	10 20 30 40 50 60 MSALLNEIQR ILGDLQSGKA ASRNKGIQQL DEKLSSCRED LDKLFLSKTS DISWTTIFEA 70 80 90 100 110 120 SKEALFKQAG NLEDVNEKVF KTLAGKNYLY DNVLEKITQF NLEAGSQTSG NGHFLAKTSI 130 140 150 160 170 180 FSAFEDGIKM RVVVKYFGDR ILSLLEKGIY SSVSYVRDLK INEYSRILSY LFELNVDMDE 190 200 210 220 230 240 VLRTRILKCI TRTVSLAKDR VQLHVDLVEY LPELSSFALS AFGARKTEIV RVYLIFASEL 250 260 270 280 290 300 AVNYNHQLNV HMQEILPKLC EYHDEDAFRD DTRNLFFQCV SKSLHSMYLK MDMCDFNTLG 310 320 330 340 350 360 VPVHEKWPQT LLRLKTIVNV EIRKNSWARC KNALLSNNKF SDPFIKMSAL AMYIVLWHLE 370 380 390 400 410 420 TKKADENGEG DAPKKIPKPA DKMETIFSLI DKKENTFNDV WLAIFTEILQ LSSVILNVAN 430 440 450 460 470 480 YQMALTTVAE IMQMYGNAKN LRNLRLCLAH LLTKEQELLH SKSIREDFLG ELWSQMANQL 490 500 510 520 530 540 ISETTTNSEE IKEKQLVLQM LIRHNKLNQK LSSTLLNNII SNEMLKRNEC LATIREIFIH 550 560 570 580 590 600 ADKCGQDKAS ADLEPIIAWA YGSADRFIAA QMIHNIDSID AQLQADTFAI SIINFLDVQQ 610 620 630 640 650 660 LRQISQSEHI VPSTERNLLA YKYNEQLICF DKDYATPFES ITHIQSETKN CLIQSNYDCL 670 680 690 700 710 720 MRGLNFEIAK ENKPAAIIKN LNSLLKLICT MERLLHYKVF DADTYTGCPL IKRIGLYLSH 730 740 750 760 770 780 IEFQLKANGA EILDKSDLPE ILRLEIYVLD VFRTNLVLLD YLERQPIEML VEFVGAALKL 790 800 810 820 830 840 HSMQRERSVD ADHGTITRLC LNILAGLCAY NSHRDEAFEH IVKVTMRWHP QDVLIVTKML 850 860 870 880 890 900 CSCQTISEAS SSWLVSKLKT LFQQHHQDAE LMDKVVQHMP TIFYFVRHKE NHLDDMLMAL 910 920 930 940 950 960 NSLLRIAIKK SYTSNLTAKI VRCVGLIAQR CPDIYLLENF AVICKSTAKF ITMPTLEVRF 970 980 990 1000 1010 1020 ATLFTFTILL ESNCVTSDAI GHSRTHWDFC QELYESIEFK KLTYNNEDAI QNSNALIVQM 1030 1040 1050 1060 1070 1080 LIAIFLRSSF HQEIALKELL HHCALRRLTE REFISLQSMS SCHRQTVRDL IRPFAAILLH 1090 1100 1110 1120 1130 1140 HWSSKRWPIS KFPYFLCYST KSEFRKTHAS EIMAYTFLYG KTEDIERCSK SISEELALPI 1150 1160 1170 1180 1190 1200 LASFLLIKNS SCSESEGQNF KEHLQLLSEN LSYSQLNATD VDLDLDILCY VISMLHDPQE 1210 1220 1230 1240 1250 1260 MMRLFGSLAI CNRTASWYSL SGESLFKCLN FHIDPEMRPS MDSRIQSMTT LQTKHSRVLV 1270 1280 1290 1300 1310 1320 DIFGRLKTNC YSASFSSQAL HDFFLYCEVA DAVYDAARKN ETIVTQCSFF VRDIWFFVVR 1330 1340 1350 1360 1370 1380 LLIHTKFIRV QMAALTFLEL LLNKHNFRLD DYQNHFGDIA KLLSNFQLCC EAKEVREKTM 1390 1400 1410 1420 1430 1440 SIVMYILESK KGHINLNSFL EETTDCEFLK PLREDCKSSQ PNIDRADVAN YLRSFLLSPT 1450 1460 1470 1480 1490 1500 PERLRDLRTY IAEHKDKVQE HEKLLFGVIN KLIQMTRDAH NKTTSIDSLK CLAQIGPLKI 1510 1520 1530 1540 1550 1560 STVSYYFQTD FEAFEKSNGE PMEAFLGVVC HSLDTSLFQF DPKTHEGLVS VAIQVVNSKP 1570 1580 1590 1600 1610 1620 GSNIMERYKN LRIFADKSTA STFLHSNKQI RRIDWLSILK ATKSLSYEPW MCAFVSKVFQ 1630 1640 1650 1660 1670 1680 MCGWLGFDKL AATSFAFAKT CLLPFIKLLL ENSLEHVESL SQMLDYFFEG FTSSTAPNSQ 1690 1700 1710 1720 1730 1740 EIFRNKRAIK KFLHICEYIR IFNNWTIPIN LSNVVMASNH CQAYFLSIMY LELWACSESP 1750 1760 1770 1780 1790 1800 KSKADFLDNE CFQDGAKKAY ESIGCLDAIP GFVNPMRSRL DFLGHGSNLS TILLESDHLD 1810 1820 1830 1840 1850 1860 RASGQLCIDI MKGNGLWSFA KLQQHQNIEP DYEIFWRLGQ WDSLTDPKHQ QNQTVVRTSL 1870 1880 1890 1900 1910 1920 DLEQEFKRHH FVALRSIGQR EEENSLSAIE QAYSCVRDIL MEISVECLQS VYKYLTWLCS 1930 1940 1950 1960 1970 1980 LQQAEDFCQI QFGTQLDPAS TTKIFRKWQT ELELKYGNFS CKEYVIAHQI ALLKLAGTRA 1990 2000 2010 2020 2030 2040 SRRMSEFYQK DPISTYLMKG IEECKSAGKL NLAAKYTATL RELPNIRESI KISVLLEDAE 2050 2060 2070 2080 2090 2100 INLKMGNQQI AKAILDYVTN NNEFVYCVQR VPALRMQGEF LLDCNAETLS WVQSHKFNNS 2110 2120 2130 2140 2150 2160 LKLIDDFVQH RQTLSEKYRD IFDWHQLDAY ASKQRTAAYA TIAKYADREY QQLHDYRHSQ 2170 2180 2190 2200 2210 2220 EYQTLKDIIE QNRQTAEKVT QRENQDRRVI SVQMKRYASL DEQQLNQIEE KLTEYLRLAL 2230 2240 2250 2260 2270 2280 TNYMAYCRLD SGFSSAAIYR IISLWFTNAT SKQCQECIKD EILTVPSYKF ICAANQLTAR 2290 2300 2310 2320 2330 2340 LNSKNTSLLK GLTDLLVQCG KDHPYHTFYQ LYPLVFAHLD GENSNTERSG IARKIIAMIC 2350 2360 2370 2380 2390 2400 EKNGTAGECS KQLESLLPAL ITFANEGKTN DNRPVSDSVR NKQFDKVRRW RNLNAVHCPT 2410 2420 2430 2440 2450 2460 LELPVMPSKE YSIISVVKWT NETTQCGGLN APVKIMCVCS DGKIRAQLVK GKDDLRQDAV 2470 2480 2490 2500 2510 2520 MQQVFGIVNE LLNQDSEFIE RKLKLRTYKV TPLSMRSGIL EWCTNSVPVG HYLVVEGKGG 2530 2540 2550 2560 2570 2580 AHARYRPNDW NNNKCRKLSS DHLKSPKETR YAIYKKICEN IKPVFHYFLL EKFPIPGVWF 2590 2600 2610 2620 2630 2640 ERRLAYTNSV ATTSMVGYVL GLGDRHTQNI LVDQQTAEVI HIDFGIAFEQ GKIQTTPETV 2650 2660 2670 2680 2690 2700 PFRLTRDFVA PMGICGTKGV FAKSCEATMH ILRRYKSVFT TILEVLLYDP LFIWGVLKKK 2710 2720 2730 2740 2750 2760 QSPQQSGEES VNLVAQRALL LVQNKLDGRE AGTMGDSNVE AQVERLINEA TLPSNLCMLF PGWDPHL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[2]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[3]	"Drosophila melanogaster MNK/Chk2 and p53 regulate multiple DNA repair and apoptotic pathways following DNA damage." Brodsky M.H., Weinert B.T., Tsang G., Rong Y.S., McGinnis N.M., Golic K.G., Rio D.C., Rubin G.M. Mol. Cell. Biol. 24:1219-1231(2004) [PubMed: 14729967] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1569-2767.
[4]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2183-2767. Strain: Berkeley. Tissue: Testis.
[5]	"ATM is required for telomere maintenance and chromosome stability during Drosophila development." Silva E., Tiong S., Pedersen M., Homola E., Royou A., Fasulo B., Siriaco G., Campbell S.D. Curr. Biol. 14:1341-1347(2004) [PubMed: 15296750] [Abstract] Cited for: FUNCTION.
[6]	"Telomere protection without a telomerase; the role of ATM and Mre11 in Drosophila telomere maintenance." Bi X., Wei S.-C.D., Rong Y.S. Curr. Biol. 14:1348-1353(2004) [PubMed: 15296751] [Abstract] Cited for: FUNCTION.
[7]	"The Drosophila ATM ortholog, dATM, mediates the response to ionizing radiation and to spontaneous DNA damage during development." Song Y.-H., Mirey G., Betson M., Haber D.A., Settleman J. Curr. Biol. 14:1354-1359(2004) [PubMed: 15296752] [Abstract] Cited for: FUNCTION, DEVELOPMENTAL STAGE.
[8]	"Drosophila atm/telomere fusion is required for telomeric localization of HP1 and telomere position effect." Oikemus S.R., McGinnis N., Queiroz-Machado J., Tukachinsky H., Takada S., Sunkel C.E., Brodsky M.H. Genes Dev. 18:1850-1861(2004) [PubMed: 15256487] [Abstract] Cited for: IDENTIFICATION, FUNCTION, DISRUPTION PHENOTYPE.
[9]	"Drosophila ATM and Mre11 are essential for the G2/M checkpoint induced by low-dose irradiation." Bi X., Gong M., Srikanta D., Rong Y.S. Genetics 171:845-847(2005) [PubMed: 16020777] [Abstract] Cited for: FUNCTION.
[10]	"Drosophila ATM and ATR checkpoint kinases control partially redundant pathways for telomere maintenance." Bi X., Srikanta D., Fanti L., Pimpinelli S., Badugu R., Kellum R., Rong Y.S. Proc. Natl. Acad. Sci. U.S.A. 102:15167-15172(2005) [PubMed: 16203987] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014297 Genomic DNA. Translation: ABI31168.1. AY395750 mRNA. Translation: AAR89513.1. AY113192 mRNA. Translation: AAM29197.1. Different initiation. BK004084 mRNA. Translation: DAA04940.1.
RefSeq	NP_001036712.1. NM_001043247.2.
3D structure databases
ProteinModelPortal	Q5EAK6.
SMR	Q5EAK6. Positions 2736-2766.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q5EAK6. 1 interaction.
STRING	Q5EAK6.
Proteomic databases
PRIDE	Q5EAK6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0110874; FBpp0110174; FBgn0045035.
GeneID	41839.
KEGG	dme:Dmel_CG6535.
Organism-specific databases
CTD	41839.
FlyBase	FBgn0045035. tefu.
Phylogenomic databases
GeneTree	EMGT00050000006666.
InParanoid	Q5EAK6.
OrthoDB	EOG4NVX15.
PhylomeDB	Q5EAK6.
Gene expression databases
Bgee	Q5EAK6.
Family and domain databases
InterPro	IPR015519. Ataxia_Telang_Mut. IPR003152. FATC. IPR011009. Kinase-like_dom. IPR000403. PI3/4_kinase_cat. IPR018936. PI3/4_kinase_CS. IPR014009. PIK_FAT. [Graphical view]
Gene3D	G3DSA:1.10.1070.11. PI3/4_kinase_cat. 2 hits.
KO	K04728.
PANTHER	PTHR11139:SF3. Ataxia_Telang_Mut. 1 hit.
Pfam	PF02260. FATC. 1 hit. PF00454. PI3_PI4_kinase. 1 hit. [Graphical view]
SMART	SM00146. PI3Kc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS51189. FAT. 1 hit. PS51190. FATC. 1 hit. PS00915. PI3_4_KINASE_1. 1 hit. PS00916. PI3_4_KINASE_2. 1 hit. PS50290. PI3_4_KINASE_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	825818.

Entry information

Entry name

ATM_DROME

Accession

Primary (citable) accession number: Q5EAK6
Secondary accession number(s): Q0KI71 , Q6TKQ1, Q8MZG4, Q9VFB1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	March 15, 2005
Last modified:	January 25, 2012
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project