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Protein

Josephin-1

Gene

JOSD1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Deubiquitinates monoubiquitinated probes (in vitro). When ubiquitinated, cleaves 'Lys-63'-linked and 'Lys-48'-linked poly-ubiquitin chains (in vitro), hence may act as a deubiquitinating enzyme. May increase macropinocytosis and suppress clathrin- and caveolae-mediated endocytosis. May enhance membrane dynamics and cell motility independently of its catalytic activity (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei36 – 361NucleophilePROSITE-ProRule annotation
Active sitei139 – 1391Proton acceptorPROSITE-ProRule annotation

GO - Molecular functioni

  1. omega peptidase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease

Keywords - Biological processi

Ubl conjugation pathway

Protein family/group databases

MEROPSiC86.004.

Names & Taxonomyi

Protein namesi
Recommended name:
Josephin-1 (EC:3.4.19.12)
Alternative name(s):
Josephin domain-containing protein 1
Gene namesi
Name:JOSD1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136 Componenti: Chromosome 5

Subcellular locationi

  1. Cell membrane By similarity
  2. Cytoplasm By similarity

  3. Note: Ubiquitination increases localization the plasma membrane. In the cytosol, the unubiquitinated form may be associated with the cytoskeleton via ACTB-binding.By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 202202Josephin-1PRO_0000053838Add
BLAST

Post-translational modificationi

Monoubiquitinated. Ubiquitination activates deubiquitination activity in vitro.By similarity

Keywords - PTMi

Ubl conjugation

Interactioni

Subunit structurei

Interacts with beta-actin/ACTB.By similarity

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini23 – 202180JosephinPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 Josephin domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG321724.
GeneTreeiENSGT00390000009228.
HOGENOMiHOG000005731.
HOVERGENiHBG039523.
InParanoidiQ5EAE5.
KOiK15235.
OMAiREVGGTY.
OrthoDBiEOG75B871.
TreeFamiTF313660.

Family and domain databases

InterProiIPR006155. Josephin.
[Graphical view]
PfamiPF02099. Josephin. 1 hit.
[Graphical view]
PROSITEiPS50957. JOSEPHIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5EAE5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSCVPWKGDK VKSESLELPQ AAPPQIYHEK QRRELCALHA LNNVFQDSNA
60 70 80 90 100
FTRETLQEIF QRLSPNTMVT PHKKSMLGNG NYDVNVIMAA LQTKGYEAVW
110 120 130 140 150
WDKRRDVGAI ALTNVMGFIM NLPSSLCWGP LKLPLKRQHW ICVREVGGAY
160 170 180 190 200
YNLDSKLKMP EWIGGKSELR KFLKHHLRGK NCELLLVVPE EVEAHQSWRA

DV
Length:202
Mass (Da):23,181
Last modified:March 15, 2005 - v1
Checksum:i8681F2DB1EAF375C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT020624 mRNA. Translation: AAX08641.1.
BC140477 mRNA. Translation: AAI40478.1.
RefSeqiNP_001014898.1. NM_001014898.2.
XP_005207376.1. XM_005207319.1.
XP_005207377.1. XM_005207320.2.
UniGeneiBt.22223.

Genome annotation databases

EnsembliENSBTAT00000013418; ENSBTAP00000013418; ENSBTAG00000010171.
GeneIDi510781.
KEGGibta:510781.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT020624 mRNA. Translation: AAX08641.1.
BC140477 mRNA. Translation: AAI40478.1.
RefSeqiNP_001014898.1. NM_001014898.2.
XP_005207376.1. XM_005207319.1.
XP_005207377.1. XM_005207320.2.
UniGeneiBt.22223.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiC86.004.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000013418; ENSBTAP00000013418; ENSBTAG00000010171.
GeneIDi510781.
KEGGibta:510781.

Organism-specific databases

CTDi9929.

Phylogenomic databases

eggNOGiNOG321724.
GeneTreeiENSGT00390000009228.
HOGENOMiHOG000005731.
HOVERGENiHBG039523.
InParanoidiQ5EAE5.
KOiK15235.
OMAiREVGGTY.
OrthoDBiEOG75B871.
TreeFamiTF313660.

Miscellaneous databases

NextBioi20869613.

Family and domain databases

InterProiIPR006155. Josephin.
[Graphical view]
PfamiPF02099. Josephin. 1 hit.
[Graphical view]
PROSITEiPS50957. JOSEPHIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Ascending colon.

Entry informationi

Entry nameiJOS1_BOVIN
AccessioniPrimary (citable) accession number: Q5EAE5
Secondary accession number(s): A5D797
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: March 15, 2005
Last modified: March 4, 2015
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.