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Q5EAE5

- JOS1_BOVIN

UniProt

Q5EAE5 - JOS1_BOVIN

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Protein
Josephin-1
Gene
JOSD1
Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Deubiquitinates monoubiquitinated probes (in vitro). When ubiquitinated, cleaves 'Lys-63'-linked and 'Lys-48'-linked poly-ubiquitin chains (in vitro), hence may act as a deubiquitinating enzyme. May increase macropinocytosis and suppress clathrin- and caveolae-mediated endocytosis. May enhance membrane dynamics and cell motility independently of its catalytic activity By similarity.

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei36 – 361Nucleophile By similarity
Active sitei139 – 1391Proton acceptor By similarity

GO - Molecular functioni

  1. omega peptidase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease

Keywords - Biological processi

Ubl conjugation pathway

Protein family/group databases

MEROPSiC86.004.

Names & Taxonomyi

Protein namesi
Recommended name:
Josephin-1 (EC:3.4.19.12)
Alternative name(s):
Josephin domain-containing protein 1
Gene namesi
Name:JOSD1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome 5

Subcellular locationi

Cell membrane By similarity. Cytoplasm By similarity
Note: Ubiquitination increases localization the plasma membrane By similarity. In the cytosol, the unubiquitinated form may be associated with the cytoskeleton via ACTB-binding By similarity.

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 202202Josephin-1
PRO_0000053838Add
BLAST

Post-translational modificationi

Monoubiquitinated By similarity. Ubiquitination activates deubiquitination activity in vitro By similarity.

Keywords - PTMi

Ubl conjugation

Interactioni

Subunit structurei

Interacts with beta-actin/ACTB By similarity.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini23 – 202180Josephin
Add
BLAST

Sequence similaritiesi

Contains 1 Josephin domain.

Phylogenomic databases

eggNOGiNOG321724.
GeneTreeiENSGT00390000009228.
HOGENOMiHOG000005731.
HOVERGENiHBG039523.
InParanoidiQ5EAE5.
KOiK15235.
OMAiREVGGTY.
OrthoDBiEOG75B871.
TreeFamiTF313660.

Family and domain databases

InterProiIPR006155. Josephin.
[Graphical view]
PfamiPF02099. Josephin. 1 hit.
[Graphical view]
PROSITEiPS50957. JOSEPHIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5EAE5-1 [UniParc]FASTAAdd to Basket

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MSCVPWKGDK VKSESLELPQ AAPPQIYHEK QRRELCALHA LNNVFQDSNA    50
FTRETLQEIF QRLSPNTMVT PHKKSMLGNG NYDVNVIMAA LQTKGYEAVW 100
WDKRRDVGAI ALTNVMGFIM NLPSSLCWGP LKLPLKRQHW ICVREVGGAY 150
YNLDSKLKMP EWIGGKSELR KFLKHHLRGK NCELLLVVPE EVEAHQSWRA 200
DV 202
Length:202
Mass (Da):23,181
Last modified:March 15, 2005 - v1
Checksum:i8681F2DB1EAF375C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BT020624 mRNA. Translation: AAX08641.1.
BC140477 mRNA. Translation: AAI40478.1.
RefSeqiNP_001014898.1. NM_001014898.2.
XP_005207376.1. XM_005207319.1.
XP_005207377.1. XM_005207320.1.
UniGeneiBt.22223.

Genome annotation databases

EnsembliENSBTAT00000013418; ENSBTAP00000013418; ENSBTAG00000010171.
GeneIDi510781.
KEGGibta:510781.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BT020624 mRNA. Translation: AAX08641.1 .
BC140477 mRNA. Translation: AAI40478.1 .
RefSeqi NP_001014898.1. NM_001014898.2.
XP_005207376.1. XM_005207319.1.
XP_005207377.1. XM_005207320.1.
UniGenei Bt.22223.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi C86.004.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSBTAT00000013418 ; ENSBTAP00000013418 ; ENSBTAG00000010171 .
GeneIDi 510781.
KEGGi bta:510781.

Organism-specific databases

CTDi 9929.

Phylogenomic databases

eggNOGi NOG321724.
GeneTreei ENSGT00390000009228.
HOGENOMi HOG000005731.
HOVERGENi HBG039523.
InParanoidi Q5EAE5.
KOi K15235.
OMAi REVGGTY.
OrthoDBi EOG75B871.
TreeFami TF313660.

Miscellaneous databases

NextBioi 20869613.

Family and domain databases

InterProi IPR006155. Josephin.
[Graphical view ]
Pfami PF02099. Josephin. 1 hit.
[Graphical view ]
PROSITEi PS50957. JOSEPHIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Ascending colon.

Entry informationi

Entry nameiJOS1_BOVIN
AccessioniPrimary (citable) accession number: Q5EAE5
Secondary accession number(s): A5D797
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: March 15, 2005
Last modified: May 14, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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