Q5EAE5 (JOS1_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Josephin-1
EC=3.4.19.12

Alternative name(s):
Josephin domain-containing protein 1

Gene names

Name:

JOSD1

Organism

Bos taurus (Bovine) [Reference proteome]

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	202 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Has low protease activity towards poly-ubiquitin chains (in vitro). May act as a deubiquitinating enzyme By similarity.
Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Sequence similarities	Contains 1 Josephin domain.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Molecular function	Hydrolase Protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	omega peptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 202	202	Josephin-1		PRO_0000053838
Regions
	Domain	23 – 202	180	Josephin
Sites
	Active site	36	1	Nucleophile By similarity
	Active site	139	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5EAE5 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: 8681F2DB1EAF375C	FASTA	202	23,181
	10 20 30 40 50 60 MSCVPWKGDK VKSESLELPQ AAPPQIYHEK QRRELCALHA LNNVFQDSNA FTRETLQEIF 70 80 90 100 110 120 QRLSPNTMVT PHKKSMLGNG NYDVNVIMAA LQTKGYEAVW WDKRRDVGAI ALTNVMGFIM 130 140 150 160 170 180 NLPSSLCWGP LKLPLKRQHW ICVREVGGAY YNLDSKLKMP EWIGGKSELR KFLKHHLRGK 190 200 NCELLLVVPE EVEAHQSWRA DV

References

[1]	"Characterization of 954 bovine full-CDS cDNA sequences." Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L. BMC Genomics 6:166-166(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[2]	NIH - Mammalian Gene Collection (MGC) project Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Ascending colon.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BT020624 mRNA. Translation: AAX08641.1. BC140477 mRNA. Translation: AAI40478.1.
IPI	IPI00692192.
RefSeq	NP_001014898.1. NM_001014898.2.
UniGene	Bt.22223.
3D structure databases
ModBase	Search...
Protein family/group databases
MEROPS	C86.004.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000013418; ENSBTAP00000013418; ENSBTAG00000010171.
GeneID	510781.
KEGG	bta:510781.
Organism-specific databases
CTD	9929.
Phylogenomic databases
eggNOG	NOG321724.
GeneTree	ENSGT00390000009228.
HOGENOM	HOG000005731.
HOVERGEN	HBG039523.
InParanoid	Q5EAE5.
KO	K15235.
OMA	IWFDKRK.
OrthoDB	EOG40P47Z.
Enzyme and pathway databases
BioCyc	CATTLE:510781-MONOMER.
Family and domain databases
InterPro	IPR006155. Josephin. [Graphical view]
Pfam	PF02099. Josephin. 1 hit. [Graphical view]
PROSITE	PS50957. JOSEPHIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20869613.

Entry information

Entry name

JOS1_BOVIN

Accession

Primary (citable) accession number: Q5EAE5
Secondary accession number(s): A5D797

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	March 15, 2005
Last modified:	May 29, 2013
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program