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Q5EAE5

- JOS1_BOVIN

UniProt

Q5EAE5 - JOS1_BOVIN

Protein

Josephin-1

Gene

JOSD1

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 53 (01 Oct 2014)
      Sequence version 1 (15 Mar 2005)
      Previous versions | rss
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    Functioni

    Deubiquitinates monoubiquitinated probes (in vitro). When ubiquitinated, cleaves 'Lys-63'-linked and 'Lys-48'-linked poly-ubiquitin chains (in vitro), hence may act as a deubiquitinating enzyme. May increase macropinocytosis and suppress clathrin- and caveolae-mediated endocytosis. May enhance membrane dynamics and cell motility independently of its catalytic activity By similarity.By similarity

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei36 – 361NucleophilePROSITE-ProRule annotation
    Active sitei139 – 1391Proton acceptorPROSITE-ProRule annotation

    GO - Molecular functioni

    1. omega peptidase activity Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease

    Keywords - Biological processi

    Ubl conjugation pathway

    Protein family/group databases

    MEROPSiC86.004.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Josephin-1 (EC:3.4.19.12)
    Alternative name(s):
    Josephin domain-containing protein 1
    Gene namesi
    Name:JOSD1
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 5

    Subcellular locationi

    Cell membrane By similarity. Cytoplasm By similarity
    Note: Ubiquitination increases localization the plasma membrane. In the cytosol, the unubiquitinated form may be associated with the cytoskeleton via ACTB-binding.By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 202202Josephin-1PRO_0000053838Add
    BLAST

    Post-translational modificationi

    Monoubiquitinated. Ubiquitination activates deubiquitination activity in vitro.By similarity

    Keywords - PTMi

    Ubl conjugation

    Interactioni

    Subunit structurei

    Interacts with beta-actin/ACTB.By similarity

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini23 – 202180JosephinPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 Josephin domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG321724.
    GeneTreeiENSGT00390000009228.
    HOGENOMiHOG000005731.
    HOVERGENiHBG039523.
    InParanoidiQ5EAE5.
    KOiK15235.
    OMAiREVGGTY.
    OrthoDBiEOG75B871.
    TreeFamiTF313660.

    Family and domain databases

    InterProiIPR006155. Josephin.
    [Graphical view]
    PfamiPF02099. Josephin. 1 hit.
    [Graphical view]
    PROSITEiPS50957. JOSEPHIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q5EAE5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSCVPWKGDK VKSESLELPQ AAPPQIYHEK QRRELCALHA LNNVFQDSNA    50
    FTRETLQEIF QRLSPNTMVT PHKKSMLGNG NYDVNVIMAA LQTKGYEAVW 100
    WDKRRDVGAI ALTNVMGFIM NLPSSLCWGP LKLPLKRQHW ICVREVGGAY 150
    YNLDSKLKMP EWIGGKSELR KFLKHHLRGK NCELLLVVPE EVEAHQSWRA 200
    DV 202
    Length:202
    Mass (Da):23,181
    Last modified:March 15, 2005 - v1
    Checksum:i8681F2DB1EAF375C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BT020624 mRNA. Translation: AAX08641.1.
    BC140477 mRNA. Translation: AAI40478.1.
    RefSeqiNP_001014898.1. NM_001014898.2.
    XP_005207376.1. XM_005207319.1.
    XP_005207377.1. XM_005207320.1.
    UniGeneiBt.22223.

    Genome annotation databases

    EnsembliENSBTAT00000013418; ENSBTAP00000013418; ENSBTAG00000010171.
    GeneIDi510781.
    KEGGibta:510781.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BT020624 mRNA. Translation: AAX08641.1 .
    BC140477 mRNA. Translation: AAI40478.1 .
    RefSeqi NP_001014898.1. NM_001014898.2.
    XP_005207376.1. XM_005207319.1.
    XP_005207377.1. XM_005207320.1.
    UniGenei Bt.22223.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi C86.004.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000013418 ; ENSBTAP00000013418 ; ENSBTAG00000010171 .
    GeneIDi 510781.
    KEGGi bta:510781.

    Organism-specific databases

    CTDi 9929.

    Phylogenomic databases

    eggNOGi NOG321724.
    GeneTreei ENSGT00390000009228.
    HOGENOMi HOG000005731.
    HOVERGENi HBG039523.
    InParanoidi Q5EAE5.
    KOi K15235.
    OMAi REVGGTY.
    OrthoDBi EOG75B871.
    TreeFami TF313660.

    Miscellaneous databases

    NextBioi 20869613.

    Family and domain databases

    InterProi IPR006155. Josephin.
    [Graphical view ]
    Pfami PF02099. Josephin. 1 hit.
    [Graphical view ]
    PROSITEi PS50957. JOSEPHIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    2. NIH - Mammalian Gene Collection (MGC) project
      Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Hereford.
      Tissue: Ascending colon.

    Entry informationi

    Entry nameiJOS1_BOVIN
    AccessioniPrimary (citable) accession number: Q5EAE5
    Secondary accession number(s): A5D797
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 6, 2005
    Last sequence update: March 15, 2005
    Last modified: October 1, 2014
    This is version 53 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3