Reviewed, UniProtKB/Swiss-Prot Q5EAD5 (PGTB1_BOVIN)

Names and origin

Protein names

Recommended name:
    Geranylgeranyl transferase type-1 subunit beta
    EC=2.5.1.59
Alternative name(s):
    Geranylgeranyl transferase type I subunit beta
      Short name=GGTase-I-beta
    Type I protein geranyl-geranyltransferase subunit beta

Gene names

Name:

PGGT1B

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	377 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the transfer of a geranyl-geranyl moiety from geranyl-geranyl pyrophosphate to a cysteine at the fourth position from the C-terminus of proteins having the C-terminal sequence Cys-aliphatic-aliphatic-X. Acts on the Rac1, Rac2, Rap1A and Rap1B proteins. The beta subunit is responsible for peptide-binding By similarity.
Catalytic activity	Geranylgeranyl diphosphate + protein-cysteine = S-geranylgeranyl-protein + diphosphate.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Heterodimer of an alpha and a beta subunit By similarity.
Sequence similarities	Belongs to the protein prenyltransferase subunit beta family. Contains 4 PFTB repeats.

Ontologies

Keywords
Domain	Repeat
Ligand	Metal-binding Zinc
Molecular function	Prenyltransferase Transferase
Gene Ontology (GO)
Molecular function	CAAX-protein geranylgeranyltransferase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 377	377	Geranylgeranyl transferase type-1 subunit beta		PRO_0000244432
Regions
	Repeat	144 – 186	43	PFTB 1
	Repeat	193 – 234	42	PFTB 2
	Repeat	245 – 284	40	PFTB 3
	Repeat	291 – 333	43	PFTB 4
Sites
	Metal binding	269	1	Zinc By similarity
	Metal binding	271	1	Zinc By similarity
	Metal binding	321	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5EAD5-1 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: 1CF78FBD41131B70	FASTA	377	42,481
	10 20 30 40 50 60 MAATEDERPT GSGEGERLDF LRDRHVRFFQ RCLQVLPERY SSLETSRLTI AFFALSGLDM 70 80 90 100 110 120 LDSLDVVNKD DIIEWIYSLQ VLPTEDRSNL NRCGFRGSSY LGIPFNPSKN PGTAHPYDSG 130 140 150 160 170 180 HIAMTYTGLS CLVILGDDLS RVNKEACLAG LRALQLEDGS FCAVPEGSEN DMRFVYCASC 190 200 210 220 230 240 ICYMLNNWSG MDMKKAINYI RRSMSYDNGL AQGAGLESHG GSTFCGIASL CLMGKLEEVF 250 260 270 280 290 300 SEKELNRIKR WCIMRQQNGY HGRPNKPVDT CYSFWVGATL KLLKIFQYTN FEKNRNYILS 310 320 330 340 350 360 TQDRLVGGFA KWPDSHPDAL HAYFGICGLS LMEESGICKV HPALNVSTRT SERLRDLHQS 370 WKTKDSKQCS ENVHIST

References

[1]	"Characterization of 954 bovine full-CDS cDNA sequences." Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L. BMC Genomics 6:166-166(2005) [PubMed: 16305752] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BT020634 mRNA. Translation: AAX08651.1.
IPI	IPI00715065.
RefSeq	NP_001015560.1.
UniGene	Bt.17618
3D structure databases
SMR	Q5EAD5. Positions 18-363.
ModBase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000002466; ENSBTAP00000002466; ENSBTAG00000001895; Bos taurus. [Genome view]
GeneID	509322.
KEGG	bta:509322.
Organism-specific databases
CTD	509322.
Phylogenomic databases
HOVERGEN	Q5EAD5.
Enzyme and pathway databases
BRENDA	2.5.1.59. 251.
Family and domain databases
InterPro	IPR001330. Prenyltrans. [Graphical view]
Pfam	PF00432. Prenyltrans. 4 hits. [Graphical view]
ProtoNet	Search...
Other Resources
BindingDB	Q5EAD5.

Entry information

Entry name

PGTB1_BOVIN

Accession

Primary (citable) accession number: Q5EAD5

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 27, 2006
Last sequence update:	March 15, 2005
Last modified:	September 1, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)