Reviewed, UniProtKB/Swiss-Prot Q5EAD4 (ACDSB_BOVIN)

Names and origin

Protein names

Recommended name:
    Short/branched chain specific acyl-CoA dehydrogenase, mitochondrial
      Short name=SBCAD
    EC=1.3.99.-
Alternative name(s):
    2-methyl branched chain acyl-CoA dehydrogenase
      Short name=2-MEBCAD
    2-methylbutyryl-coenzyme A dehydrogenase
      Short name=2-methylbutyryl-CoA dehydrogenase

Gene names

Name:

ACADSB

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	432 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Has greatest activity toward short branched chain acyl-CoA derivative such as (s)-2-methylbutyryl-CoA, isobutyryl-CoA, and 2-methylhexanoyl-CoA as well as toward short straight chain acyl-CoAs such as butyryl-CoA and hexanoyl-CoA. Can use valproyl-CoA as substrate and may play a role in controlling the metabolic flux of valproic acid in the development of toxicity of this agent By similarity.
Catalytic activity	Acyl-CoA + ETF = 2,3-dehydroacyl-CoA + reduced ETF.
Cofactor	FAD By similarity.
Pathway	Lipid metabolism; mitochondrial fatty acid beta-oxidation.
Subunit structure	Homotetramer By similarity.
Subcellular location	Mitochondrion matrix Probable.
Sequence similarities	Belongs to the acyl-CoA dehydrogenase family.

Ontologies

Keywords
Biological process	Fatty acid metabolism Lipid metabolism
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
PTM	Acetylation
Gene Ontology (GO)
Biological process	fatty acid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro acyl-CoA dehydrogenase activity Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 33	33	Mitochondrion By similarity
	Chain	34 – 432	399	Short/branched chain specific acyl-CoA dehydrogenase, mitochondrial		PRO_0000281991
Sites
	Active site	414	1	Proton acceptor By similarity
Amino acid modifications
	Modified residue	284	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5EAD4-1 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: E1C84A97A3EDC1C6	FASTA	432	47,123
	10 20 30 40 50 60 MERATVRLLR GGALLRRNFP SCLSSWKTPP HALNSSQSEA QLKATSNGPP LAPLQTFTDE 70 80 90 100 110 120 EMMIKSAVKK FAQEQVAPFV SKMDEDSKME KSVIQGLFQQ GLMGIEIDTK YGGTGASFFS 130 140 150 160 170 180 SVLVIEELAK VDASVALVCD IQNTLINRMI GKYGTEEQKA TYLPKLATEK ASSICISETG 190 200 210 220 230 240 AGSDSFAMKT RADKKGDYYI INGSKMWISS AEIAGLFVVM ANADFSAGYK GITCFLVDGD 250 260 270 280 290 300 TEGLHVGKPE NKLGIRASST CPVTFENVKV PKTNILGQVG HGYKYAIGSL NEGRIGIAAQ 310 320 330 340 350 360 MLGVAQGCFD YTIPYIKERK QFGRRVFDFQ GLQHQVAHMA TQLEAARLLT YNAARLLEAG 370 380 390 400 410 420 RPMIKEASMA KYHASELAGL ITSKCIEWMG GVGYTKSYPV EKYFRDAKIG TIYEGTSNIQ 430 LNTIAKCISA EY

References

[1]	"Characterization of 954 bovine full-CDS cDNA sequences." Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L. BMC Genomics 6:166-166(2005) [PubMed: 16305752] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[2]	NIH - Mammalian Gene Collection (MGC) project Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Hippocampus.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC122698 mRNA. Translation: AAI22699.1. BT020635 mRNA. Translation: AAX08652.1.
IPI	IPI00717195.
RefSeq	NP_001017933.1.
UniGene	Bt.23226
3D structure databases
SMR	Q5EAD4. Positions 52-432.
ModBase	Search...
Genome annotation databases
Ensembl	ENSBTAG00000018041. Bos taurus. [Contig view]
GeneID	504301.
KEGG	bta:504301.
Phylogenomic databases
HOVERGEN	Q5EAD4.
OMA	Q5EAD4. IEWMGGV.
Family and domain databases
InterPro	IPR006089. Acyl-CoA_DH_CS. IPR006092. Acyl-CoA_DH_N. IPR006090. Acyl-CoA_Oxase/DH_1. IPR006091. Acyl-CoA_Oxase/DH_M. IPR013786. AcylCoA_DH/ox_N. IPR013764. AcylCoA_oxidase/DH_1/2_C. [Graphical view]
Gene3D	G3DSA:2.40.110.10. Acyl_CoA_DH/ox_M. 1 hit. G3DSA:1.10.540.10. AcylCoA_DH/ox_N. 1 hit. G3DSA:1.20.140.10. AcylCoA_DH_1/2_C. 1 hit.
Pfam	PF00441. Acyl-CoA_dh_1. 1 hit. PF02770. Acyl-CoA_dh_M. 1 hit. PF02771. Acyl-CoA_dh_N. 1 hit. [Graphical view]
PROSITE	PS00072. ACYL_COA_DH_1. 1 hit. PS00073. ACYL_COA_DH_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACDSB_BOVIN

Accession

Primary (citable) accession number: Q5EAD4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 3, 2007
Last sequence update:	March 15, 2005
Last modified:	June 16, 2009
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)