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Protein

Short/branched chain specific acyl-CoA dehydrogenase, mitochondrial

Gene

ACADSB

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Has greatest activity toward short branched chain acyl-CoA derivative such as (s)-2-methylbutyryl-CoA, isobutyryl-CoA, and 2-methylhexanoyl-CoA as well as toward short straight chain acyl-CoAs such as butyryl-CoA and hexanoyl-CoA. Can use valproyl-CoA as substrate and may play a role in controlling the metabolic flux of valproic acid in the development of toxicity of this agent (By similarity).By similarity

Catalytic activityi

Acyl-CoA + acceptor = 2,3-dehydroacyl-CoA + reduced acceptor.
2-methylbutanoyl-CoA + electron-transfer flavoprotein = (E)-2-methylbut-2-enoyl-CoA + reduced electron-transfer flavoprotein + H+.

Cofactori

FADBy similarity

Pathwayi: mitochondrial fatty acid beta-oxidation

This protein is involved in the pathway mitochondrial fatty acid beta-oxidation, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway mitochondrial fatty acid beta-oxidation and in Lipid metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei183 – 1831Substrate; via carbonyl oxygenBy similarity
Binding sitei283 – 2831SubstrateBy similarity
Binding sitei319 – 3191FAD; shared with dimeric partnerBy similarity
Binding sitei330 – 3301FAD; shared with dimeric partnerBy similarity
Active sitei414 – 4141Proton acceptorBy similarity
Binding sitei415 – 4151Substrate; via amide nitrogenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi174 – 18310FADBy similarity
Nucleotide bindingi207 – 2093FADBy similarity
Nucleotide bindingi387 – 3915FAD; shared with dimeric partnerBy similarity
Nucleotide bindingi416 – 4183FADBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Fatty acid metabolism, Lipid metabolism

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

ReactomeiR-BTA-70895. Branched-chain amino acid catabolism.
UniPathwayiUPA00660.

Names & Taxonomyi

Protein namesi
Recommended name:
Short/branched chain specific acyl-CoA dehydrogenase, mitochondrial (EC:1.3.8.5)
Short name:
SBCAD
Alternative name(s):
2-methyl branched chain acyl-CoA dehydrogenase
Short name:
2-MEBCAD
2-methylbutyryl-coenzyme A dehydrogenase
Short name:
2-methylbutyryl-CoA dehydrogenase
Gene namesi
Name:ACADSB
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 26

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3333MitochondrionBy similarityAdd
BLAST
Chaini34 – 432399Short/branched chain specific acyl-CoA dehydrogenase, mitochondrialPRO_0000281991Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei70 – 701N6-acetyllysine; alternateBy similarity
Modified residuei70 – 701N6-succinyllysine; alternateBy similarity
Modified residuei183 – 1831PhosphoserineBy similarity
Modified residuei284 – 2841N6-acetyllysine; alternateBy similarity
Modified residuei284 – 2841N6-succinyllysine; alternateBy similarity
Modified residuei426 – 4261N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ5EAD4.
PRIDEiQ5EAD4.

Expressioni

Gene expression databases

BgeeiENSBTAG00000018041.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000024017.

Structurei

3D structure databases

ProteinModelPortaliQ5EAD4.
SMRiQ5EAD4. Positions 52-432.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni229 – 2302Substrate bindingBy similarity
Regioni291 – 2944Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the acyl-CoA dehydrogenase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0139. Eukaryota.
COG1960. LUCA.
GeneTreeiENSGT00760000119007.
HOGENOMiHOG000131659.
HOVERGENiHBG000224.
InParanoidiQ5EAD4.
KOiK09478.
OMAiGQTTSKC.
OrthoDBiEOG091G04BS.
TreeFamiTF105055.

Family and domain databases

Gene3Di1.10.540.10. 1 hit.
InterProiIPR006089. Acyl-CoA_DH_CS.
IPR006091. Acyl-CoA_Oxase/DH_cen-dom.
IPR009075. AcylCo_DH/oxidase_C.
IPR013786. AcylCoA_DH/ox_N.
IPR009100. AcylCoA_DH/oxidase_NM_dom.
[Graphical view]
PfamiPF00441. Acyl-CoA_dh_1. 1 hit.
PF02770. Acyl-CoA_dh_M. 1 hit.
PF02771. Acyl-CoA_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF47203. SSF47203. 1 hit.
SSF56645. SSF56645. 1 hit.
PROSITEiPS00072. ACYL_COA_DH_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5EAD4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MERATVRLLR GGALLRRNFP SCLSSWKTPP HALNSSQSEA QLKATSNGPP
60 70 80 90 100
LAPLQTFTDE EMMIKSAVKK FAQEQVAPFV SKMDEDSKME KSVIQGLFQQ
110 120 130 140 150
GLMGIEIDTK YGGTGASFFS SVLVIEELAK VDASVALVCD IQNTLINRMI
160 170 180 190 200
GKYGTEEQKA TYLPKLATEK ASSICISETG AGSDSFAMKT RADKKGDYYI
210 220 230 240 250
INGSKMWISS AEIAGLFVVM ANADFSAGYK GITCFLVDGD TEGLHVGKPE
260 270 280 290 300
NKLGIRASST CPVTFENVKV PKTNILGQVG HGYKYAIGSL NEGRIGIAAQ
310 320 330 340 350
MLGVAQGCFD YTIPYIKERK QFGRRVFDFQ GLQHQVAHMA TQLEAARLLT
360 370 380 390 400
YNAARLLEAG RPMIKEASMA KYHASELAGL ITSKCIEWMG GVGYTKSYPV
410 420 430
EKYFRDAKIG TIYEGTSNIQ LNTIAKCISA EY
Length:432
Mass (Da):47,123
Last modified:March 15, 2005 - v1
Checksum:iE1C84A97A3EDC1C6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC122698 mRNA. Translation: AAI22699.1.
BT020635 mRNA. Translation: AAX08652.1.
RefSeqiNP_001017933.1. NM_001017933.1.
UniGeneiBt.23226.

Genome annotation databases

EnsembliENSBTAT00000024017; ENSBTAP00000024017; ENSBTAG00000018041.
GeneIDi504301.
KEGGibta:504301.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC122698 mRNA. Translation: AAI22699.1.
BT020635 mRNA. Translation: AAX08652.1.
RefSeqiNP_001017933.1. NM_001017933.1.
UniGeneiBt.23226.

3D structure databases

ProteinModelPortaliQ5EAD4.
SMRiQ5EAD4. Positions 52-432.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000024017.

Proteomic databases

PaxDbiQ5EAD4.
PRIDEiQ5EAD4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000024017; ENSBTAP00000024017; ENSBTAG00000018041.
GeneIDi504301.
KEGGibta:504301.

Organism-specific databases

CTDi36.

Phylogenomic databases

eggNOGiKOG0139. Eukaryota.
COG1960. LUCA.
GeneTreeiENSGT00760000119007.
HOGENOMiHOG000131659.
HOVERGENiHBG000224.
InParanoidiQ5EAD4.
KOiK09478.
OMAiGQTTSKC.
OrthoDBiEOG091G04BS.
TreeFamiTF105055.

Enzyme and pathway databases

UniPathwayiUPA00660.
ReactomeiR-BTA-70895. Branched-chain amino acid catabolism.

Gene expression databases

BgeeiENSBTAG00000018041.

Family and domain databases

Gene3Di1.10.540.10. 1 hit.
InterProiIPR006089. Acyl-CoA_DH_CS.
IPR006091. Acyl-CoA_Oxase/DH_cen-dom.
IPR009075. AcylCo_DH/oxidase_C.
IPR013786. AcylCoA_DH/ox_N.
IPR009100. AcylCoA_DH/oxidase_NM_dom.
[Graphical view]
PfamiPF00441. Acyl-CoA_dh_1. 1 hit.
PF02770. Acyl-CoA_dh_M. 1 hit.
PF02771. Acyl-CoA_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF47203. SSF47203. 1 hit.
SSF56645. SSF56645. 1 hit.
PROSITEiPS00072. ACYL_COA_DH_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiACDSB_BOVIN
AccessioniPrimary (citable) accession number: Q5EAD4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: March 15, 2005
Last modified: September 7, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.