Reviewed, UniProtKB/Swiss-Prot Q5EAD2 (SERA_BOVIN)

Names and origin

Protein names

Recommended name:
    D-3-phosphoglycerate dehydrogenase
      Short name=3-PGDH
    EC=1.1.1.95

Gene names

Name:

PHGDH

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	533 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	3-phospho-D-glycerate + NAD+ = 3-phosphonooxypyruvate + NADH. 2-hydroxyglutarate + NAD+ = 2-oxoglutarate + NADH.
Pathway	Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glyceric acid: step 1/3.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Serine biosynthesis
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Acetylation
Gene Ontology (GO)
Biological process	L-serine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro phosphoglycerate dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 533	532	D-3-phosphoglycerate dehydrogenase		PRO_0000076011
Sites
	Active site	236	1	By similarity
	Active site	265	1	By similarity
	Active site	283	1	Proton donor By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylalanine By similarity
Experimental info
	Sequence conflict	414 – 415	2	RE → GK in AAI05480. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q5EAD2-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 9351034A033A2750	FASTA	533	56,452
	10 20 30 40 50 60 MAFANLRKVL ISDSLDPCCR KILQDGGLQV VEKQNLSKEE LIAELQDCEG LIVRSATKVT 70 80 90 100 110 120 SDIINAAEKL QVVGRAGTGV DNVDLEAATR KGILVMNTPN GNSLSAAELT CGMIMCLARQ 130 140 150 160 170 180 IPQAAASMKD GKWERKKFMG TELNGKVLGI LGLGRIGREV ATRMQSFGMK TIGYDPIISP 190 200 210 220 230 240 EVSASFGVQQ LPLEQIWPLC DFITVHTPLL PSTTGLLNDS TFAQCKKGVC VVNCARGGIV 250 260 270 280 290 300 DEGALLRALQ SGQCAGAALD VFTEEPPRDR ALVNHENVIS CPHLGASTKE AQSRCGEEIA 310 320 330 340 350 360 LQFVDMVKGK ALAGVVNAQA LASIFCPHTK PWVSLAKALG ALMQAWAGSP KGAIQVVTQG 370 380 390 400 410 420 SSLKNSGSCL APAVIIGLLK DASQQSNVNL VNAMLLVKEA GLDVTTSHNP ATPREQDFGE 430 440 450 460 470 480 CLLTVALAGA PYQAVGLVQG TKPVLQALNG AVFRPEVPLH PGQPLLMFRA QASNPAMLPT 490 500 510 520 530 MIGLLAEAGV QLLSYQSSVV SDGETWHVMS ISSLLPSLAP WKPHVTEAFQ FCF

References

[1]	"Characterization of 954 bovine full-CDS cDNA sequences." Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L. BMC Genomics 6:166-166(2005) [PubMed: 16305752] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[2]	NIH - Mammalian Gene Collection (MGC) project Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Hypothalamus.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BT020637 mRNA. Translation: AAX08654.1. BC105479 mRNA. Translation: AAI05480.1.
IPI	IPI00699717.
RefSeq	NP_001030189.1.
UniGene	Bt.53026
3D structure databases
SMR	Q5EAD2. Positions 5-306, 6-307.
ModBase	Search...
Genome annotation databases
Ensembl	ENSBTAG00000039719. Bos taurus. [Contig view]
GeneID	505103.
KEGG	bta:505103.
Phylogenomic databases
HOVERGEN	Q5EAD2.
Enzyme and pathway databases
BRENDA	1.1.1.95. 251.
Family and domain databases
InterPro	IPR006236. D-3-Phosphoglycerate_DH. IPR006139. D-isomer_2_OHA_DH. IPR006140. D-isomer_2_OHA_DH_NAD-bd. IPR015508. D3PG_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR10996:SF20. D3PG_Deh. 1 hit.
Pfam	PF00389. 2-Hacid_dh. 1 hit. PF02826. 2-Hacid_dh_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR01327. PGDH. 1 hit.
PROSITE	PS00065. D_2_HYDROXYACID_DH_1. 1 hit. PS00670. D_2_HYDROXYACID_DH_2. 1 hit. PS00671. D_2_HYDROXYACID_DH_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SERA_BOVIN

Accession

Primary (citable) accession number: Q5EAD2
Secondary accession number(s): Q2KJ79

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 36 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)