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Q5EAC2

- GSHB_BOVIN

UniProt

Q5EAC2 - GSHB_BOVIN

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Protein

Glutathione synthetase

Gene

GSS

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.

Cofactori

Binds 1 magnesium ion per subunit.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei125 – 1251SubstrateBy similarity
Metal bindingi144 – 1441MagnesiumBy similarity
Binding sitei144 – 1441ATPBy similarity
Metal bindingi146 – 1461MagnesiumBy similarity
Binding sitei220 – 2201SubstrateBy similarity
Binding sitei305 – 3051ATPBy similarity
Metal bindingi368 – 3681MagnesiumBy similarity
Binding sitei375 – 3751ATPBy similarity
Binding sitei425 – 4251ATPBy similarity
Binding sitei450 – 4501SubstrateBy similarity
Binding sitei452 – 4521ATPBy similarity
Binding sitei458 – 4581ATP; via carbonyl oxygenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi364 – 37310ATPBy similarity
Nucleotide bindingi398 – 4014ATPBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB
  2. glutathione binding Source: UniProtKB
  3. glutathione synthase activity Source: UniProtKB-EC
  4. magnesium ion binding Source: UniProtKB
  5. protein homodimerization activity Source: UniProtKB

GO - Biological processi

  1. response to cadmium ion Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Glutathione biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_227316. Glutathione synthesis and recycling.
UniPathwayiUPA00142; UER00210.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione synthetase (EC:6.3.2.3)
Short name:
GSH synthetase
Short name:
GSH-S
Alternative name(s):
Glutathione synthase
Gene namesi
Name:GSS
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome 13

Subcellular locationi

GO - Cellular componenti

  1. extracellular vesicular exosome Source: Ensembl
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 474473Glutathione synthetasePRO_0000247549Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ5EAC2.
PRIDEiQ5EAC2.

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ5EAC2.
SMRiQ5EAC2. Positions 3-474.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni148 – 1514Substrate bindingBy similarity
Regioni214 – 2163Substrate bindingBy similarity
Regioni267 – 2704Substrate bindingBy similarity
Regioni461 – 4622Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the eukaryotic GSH synthase family.Curated

Phylogenomic databases

eggNOGiNOG329040.
GeneTreeiENSGT00390000013764.
HOGENOMiHOG000172641.
HOVERGENiHBG002458.
InParanoidiQ5EAC2.
KOiK01920.
OrthoDBiEOG757CXD.
TreeFamiTF105187.

Family and domain databases

Gene3Di1.10.1080.10. 2 hits.
3.30.1490.50. 1 hit.
3.30.1490.80. 2 hits.
3.40.50.1760. 1 hit.
InterProiIPR004887. Glutathione_synth_subst-bd_euk.
IPR014042. Glutathione_synthase_a-hlx_euk.
IPR014709. Glutathione_synthase_dom.
IPR005615. Glutathione_synthase_euk.
IPR014049. Glutathione_synthase_N_euk.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERiPTHR11130. PTHR11130. 1 hit.
PfamiPF03917. GSH_synth_ATP. 1 hit.
PF03199. GSH_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF001558. GSHase. 1 hit.
SUPFAMiSSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01986. glut_syn_euk. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5EAC2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MATGWGSLLQ DEQQLEELAR QAVDRALAEG VLLRTSQAPS SSHVVSYAPF
60 70 80 90 100
TLFPSPVPSA LLEQAYAVQA DFNLLVDAVS QNAVFLEQTL SSTIKRDSFT
110 120 130 140 150
ARLFDIHKQV LKEGIAQTVF LGLNRSDYMF QCNPDGSAAL KQIEINTVSA
160 170 180 190 200
SFGGLASRTP AVHRHVLSVL GKTKEAAKIL SNNPSKGLAM GIAKAWELYG
210 220 230 240 250
SANAQVLLIA QEKERNIFDQ RAIENELLAR NIHVIRRKFE DVSEKGSLDQ
260 270 280 290 300
DRRLFMDGQE IAVVYFRDGY MPGHYSLQNW EARLLLERSC AVKCPDIATQ
310 320 330 340 350
LAGTKKVQQE LSRVGVLESF LPGQPEAVAR LRATFAGLYS LDLGEEGDQA
360 370 380 390 400
ITKAIAAPSC FVLKPQREGG GNNLYGEEMV QALERLKDSE ERASYILMEK
410 420 430 440 450
IEPEPFRNCL LRPGSPARVI QCISELGIFG VYVREGKTLV MNKHVGHLLR
460 470
TKAIEHADGG VAAGVAVLDN PYPV
Length:474
Mass (Da):52,066
Last modified:March 15, 2005 - v1
Checksum:i964C08AF6A93FFF8
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti238 – 2381K → R in AAI09714. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BT020647 mRNA. Translation: AAX08664.1.
BC109713 mRNA. Translation: AAI09714.1.
RefSeqiNP_001015630.1. NM_001015630.1.
XP_005214785.1. XM_005214728.1.
UniGeneiBt.9056.

Genome annotation databases

EnsembliENSBTAT00000004559; ENSBTAP00000004559; ENSBTAG00000003504.
GeneIDi525059.
KEGGibta:525059.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BT020647 mRNA. Translation: AAX08664.1 .
BC109713 mRNA. Translation: AAI09714.1 .
RefSeqi NP_001015630.1. NM_001015630.1.
XP_005214785.1. XM_005214728.1.
UniGenei Bt.9056.

3D structure databases

ProteinModelPortali Q5EAC2.
SMRi Q5EAC2. Positions 3-474.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PaxDbi Q5EAC2.
PRIDEi Q5EAC2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSBTAT00000004559 ; ENSBTAP00000004559 ; ENSBTAG00000003504 .
GeneIDi 525059.
KEGGi bta:525059.

Organism-specific databases

CTDi 2937.

Phylogenomic databases

eggNOGi NOG329040.
GeneTreei ENSGT00390000013764.
HOGENOMi HOG000172641.
HOVERGENi HBG002458.
InParanoidi Q5EAC2.
KOi K01920.
OrthoDBi EOG757CXD.
TreeFami TF105187.

Enzyme and pathway databases

UniPathwayi UPA00142 ; UER00210 .
Reactomei REACT_227316. Glutathione synthesis and recycling.

Miscellaneous databases

NextBioi 20874086.

Family and domain databases

Gene3Di 1.10.1080.10. 2 hits.
3.30.1490.50. 1 hit.
3.30.1490.80. 2 hits.
3.40.50.1760. 1 hit.
InterProi IPR004887. Glutathione_synth_subst-bd_euk.
IPR014042. Glutathione_synthase_a-hlx_euk.
IPR014709. Glutathione_synthase_dom.
IPR005615. Glutathione_synthase_euk.
IPR014049. Glutathione_synthase_N_euk.
IPR016185. PreATP-grasp_dom.
[Graphical view ]
PANTHERi PTHR11130. PTHR11130. 1 hit.
Pfami PF03917. GSH_synth_ATP. 1 hit.
PF03199. GSH_synthase. 1 hit.
[Graphical view ]
PIRSFi PIRSF001558. GSHase. 1 hit.
SUPFAMi SSF52440. SSF52440. 1 hit.
TIGRFAMsi TIGR01986. glut_syn_euk. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Crossbred X Angus.
    Tissue: Liver.

Entry informationi

Entry nameiGSHB_BOVIN
AccessioniPrimary (citable) accession number: Q5EAC2
Secondary accession number(s): Q2TBS8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: March 15, 2005
Last modified: October 29, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3