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Protein

Glutathione synthetase

Gene

GSS

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Pathway:iglutathione biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes glutathione from L-cysteine and L-glutamate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamate--cysteine ligase regulatory subunit (GCLM)
  2. Glutathione synthetase (GSS)
This subpathway is part of the pathway glutathione biosynthesis, which is itself part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes glutathione from L-cysteine and L-glutamate, the pathway glutathione biosynthesis and in Sulfur metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei125 – 1251SubstrateBy similarity
Metal bindingi144 – 1441MagnesiumBy similarity
Binding sitei144 – 1441ATPBy similarity
Metal bindingi146 – 1461MagnesiumBy similarity
Binding sitei220 – 2201SubstrateBy similarity
Binding sitei305 – 3051ATPBy similarity
Metal bindingi368 – 3681MagnesiumBy similarity
Binding sitei375 – 3751ATPBy similarity
Binding sitei425 – 4251ATPBy similarity
Binding sitei450 – 4501SubstrateBy similarity
Binding sitei452 – 4521ATPBy similarity
Binding sitei458 – 4581ATP; via carbonyl oxygenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi364 – 37310ATPBy similarity
Nucleotide bindingi398 – 4014ATPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Glutathione biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_292864. Glutathione synthesis and recycling.
UniPathwayiUPA00142; UER00210.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione synthetase (EC:6.3.2.3)
Short name:
GSH synthetase
Short name:
GSH-S
Alternative name(s):
Glutathione synthase
Gene namesi
Name:GSS
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 474473Glutathione synthetasePRO_0000247549Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei415 – 4151PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ5EAC2.
PRIDEiQ5EAC2.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000004559.

Structurei

3D structure databases

ProteinModelPortaliQ5EAC2.
SMRiQ5EAC2. Positions 3-474.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni148 – 1514Substrate bindingBy similarity
Regioni214 – 2163Substrate bindingBy similarity
Regioni267 – 2704Substrate bindingBy similarity
Regioni461 – 4622Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the eukaryotic GSH synthase family.Curated

Phylogenomic databases

eggNOGiNOG329040.
HOGENOMiHOG000172641.
HOVERGENiHBG002458.
InParanoidiQ5EAC2.
KOiK01920.
OrthoDBiEOG757CXD.
TreeFamiTF105187.

Family and domain databases

Gene3Di1.10.1080.10. 2 hits.
3.30.1490.50. 1 hit.
3.30.1490.80. 2 hits.
3.40.50.1760. 1 hit.
InterProiIPR004887. Glutathione_synth_subst-bd_euk.
IPR014042. Glutathione_synthase_a-hlx_euk.
IPR014709. Glutathione_synthase_dom.
IPR005615. Glutathione_synthase_euk.
IPR014049. Glutathione_synthase_N_euk.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERiPTHR11130. PTHR11130. 1 hit.
PfamiPF03917. GSH_synth_ATP. 1 hit.
PF03199. GSH_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF001558. GSHase. 1 hit.
SUPFAMiSSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01986. glut_syn_euk. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5EAC2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATGWGSLLQ DEQQLEELAR QAVDRALAEG VLLRTSQAPS SSHVVSYAPF
60 70 80 90 100
TLFPSPVPSA LLEQAYAVQA DFNLLVDAVS QNAVFLEQTL SSTIKRDSFT
110 120 130 140 150
ARLFDIHKQV LKEGIAQTVF LGLNRSDYMF QCNPDGSAAL KQIEINTVSA
160 170 180 190 200
SFGGLASRTP AVHRHVLSVL GKTKEAAKIL SNNPSKGLAM GIAKAWELYG
210 220 230 240 250
SANAQVLLIA QEKERNIFDQ RAIENELLAR NIHVIRRKFE DVSEKGSLDQ
260 270 280 290 300
DRRLFMDGQE IAVVYFRDGY MPGHYSLQNW EARLLLERSC AVKCPDIATQ
310 320 330 340 350
LAGTKKVQQE LSRVGVLESF LPGQPEAVAR LRATFAGLYS LDLGEEGDQA
360 370 380 390 400
ITKAIAAPSC FVLKPQREGG GNNLYGEEMV QALERLKDSE ERASYILMEK
410 420 430 440 450
IEPEPFRNCL LRPGSPARVI QCISELGIFG VYVREGKTLV MNKHVGHLLR
460 470
TKAIEHADGG VAAGVAVLDN PYPV
Length:474
Mass (Da):52,066
Last modified:March 15, 2005 - v1
Checksum:i964C08AF6A93FFF8
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti238 – 2381K → R in AAI09714 (Ref. 2) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT020647 mRNA. Translation: AAX08664.1.
BC109713 mRNA. Translation: AAI09714.1.
RefSeqiNP_001015630.1. NM_001015630.1.
XP_005214785.1. XM_005214728.2.
UniGeneiBt.9056.

Genome annotation databases

GeneIDi525059.
KEGGibta:525059.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT020647 mRNA. Translation: AAX08664.1.
BC109713 mRNA. Translation: AAI09714.1.
RefSeqiNP_001015630.1. NM_001015630.1.
XP_005214785.1. XM_005214728.2.
UniGeneiBt.9056.

3D structure databases

ProteinModelPortaliQ5EAC2.
SMRiQ5EAC2. Positions 3-474.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000004559.

Proteomic databases

PaxDbiQ5EAC2.
PRIDEiQ5EAC2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi525059.
KEGGibta:525059.

Organism-specific databases

CTDi2937.

Phylogenomic databases

eggNOGiNOG329040.
HOGENOMiHOG000172641.
HOVERGENiHBG002458.
InParanoidiQ5EAC2.
KOiK01920.
OrthoDBiEOG757CXD.
TreeFamiTF105187.

Enzyme and pathway databases

UniPathwayiUPA00142; UER00210.
ReactomeiREACT_292864. Glutathione synthesis and recycling.

Miscellaneous databases

NextBioi20874086.

Family and domain databases

Gene3Di1.10.1080.10. 2 hits.
3.30.1490.50. 1 hit.
3.30.1490.80. 2 hits.
3.40.50.1760. 1 hit.
InterProiIPR004887. Glutathione_synth_subst-bd_euk.
IPR014042. Glutathione_synthase_a-hlx_euk.
IPR014709. Glutathione_synthase_dom.
IPR005615. Glutathione_synthase_euk.
IPR014049. Glutathione_synthase_N_euk.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERiPTHR11130. PTHR11130. 1 hit.
PfamiPF03917. GSH_synth_ATP. 1 hit.
PF03199. GSH_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF001558. GSHase. 1 hit.
SUPFAMiSSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01986. glut_syn_euk. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Crossbred X Angus.
    Tissue: Liver.

Entry informationi

Entry nameiGSHB_BOVIN
AccessioniPrimary (citable) accession number: Q5EAC2
Secondary accession number(s): Q2TBS8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: March 15, 2005
Last modified: June 24, 2015
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.