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Q5EAC2

- GSHB_BOVIN

UniProt

Q5EAC2 - GSHB_BOVIN

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Protein
Glutathione synthetase
Gene
GSS
Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.

Cofactori

Binds 1 magnesium ion per subunit By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei125 – 1251Substrate By similarity
Metal bindingi144 – 1441Magnesium By similarity
Binding sitei144 – 1441ATP By similarity
Metal bindingi146 – 1461Magnesium By similarity
Binding sitei220 – 2201Substrate By similarity
Binding sitei305 – 3051ATP By similarity
Metal bindingi368 – 3681Magnesium By similarity
Binding sitei375 – 3751ATP By similarity
Binding sitei425 – 4251ATP By similarity
Binding sitei450 – 4501Substrate By similarity
Binding sitei452 – 4521ATP By similarity
Binding sitei458 – 4581ATP; via carbonyl oxygen By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi364 – 37310ATP By similarity
Nucleotide bindingi398 – 4014ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB
  2. glutathione binding Source: UniProtKB
  3. glutathione synthase activity Source: UniProtKB-EC
  4. magnesium ion binding Source: UniProtKB
  5. protein homodimerization activity Source: UniProtKB

GO - Biological processi

  1. response to cadmium ion Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Glutathione biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_227316. Glutathione synthesis and recycling.
UniPathwayiUPA00142; UER00210.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione synthetase (EC:6.3.2.3)
Short name:
GSH synthetase
Short name:
GSH-S
Alternative name(s):
Glutathione synthase
Gene namesi
Name:GSS
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome 13

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 474473Glutathione synthetase
PRO_0000247549Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine By similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ5EAC2.
PRIDEiQ5EAC2.

Interactioni

Subunit structurei

Homodimer By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ5EAC2.
SMRiQ5EAC2. Positions 3-474.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni148 – 1514Substrate binding By similarity
Regioni214 – 2163Substrate binding By similarity
Regioni267 – 2704Substrate binding By similarity
Regioni461 – 4622Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG329040.
GeneTreeiENSGT00390000013764.
HOGENOMiHOG000172641.
HOVERGENiHBG002458.
InParanoidiQ5EAC2.
KOiK01920.
OrthoDBiEOG757CXD.
TreeFamiTF105187.

Family and domain databases

Gene3Di1.10.1080.10. 2 hits.
3.30.1490.50. 1 hit.
3.30.1490.80. 2 hits.
3.40.50.1760. 1 hit.
InterProiIPR004887. Glutathione_synth_subst-bd_euk.
IPR014042. Glutathione_synthase_a-hlx_euk.
IPR014709. Glutathione_synthase_dom.
IPR005615. Glutathione_synthase_euk.
IPR014049. Glutathione_synthase_N_euk.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERiPTHR11130. PTHR11130. 1 hit.
PfamiPF03917. GSH_synth_ATP. 1 hit.
PF03199. GSH_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF001558. GSHase. 1 hit.
SUPFAMiSSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01986. glut_syn_euk. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5EAC2-1 [UniParc]FASTAAdd to Basket

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MATGWGSLLQ DEQQLEELAR QAVDRALAEG VLLRTSQAPS SSHVVSYAPF    50
TLFPSPVPSA LLEQAYAVQA DFNLLVDAVS QNAVFLEQTL SSTIKRDSFT 100
ARLFDIHKQV LKEGIAQTVF LGLNRSDYMF QCNPDGSAAL KQIEINTVSA 150
SFGGLASRTP AVHRHVLSVL GKTKEAAKIL SNNPSKGLAM GIAKAWELYG 200
SANAQVLLIA QEKERNIFDQ RAIENELLAR NIHVIRRKFE DVSEKGSLDQ 250
DRRLFMDGQE IAVVYFRDGY MPGHYSLQNW EARLLLERSC AVKCPDIATQ 300
LAGTKKVQQE LSRVGVLESF LPGQPEAVAR LRATFAGLYS LDLGEEGDQA 350
ITKAIAAPSC FVLKPQREGG GNNLYGEEMV QALERLKDSE ERASYILMEK 400
IEPEPFRNCL LRPGSPARVI QCISELGIFG VYVREGKTLV MNKHVGHLLR 450
TKAIEHADGG VAAGVAVLDN PYPV 474
Length:474
Mass (Da):52,066
Last modified:March 15, 2005 - v1
Checksum:i964C08AF6A93FFF8
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti238 – 2381K → R in AAI09714. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BT020647 mRNA. Translation: AAX08664.1.
BC109713 mRNA. Translation: AAI09714.1.
RefSeqiNP_001015630.1. NM_001015630.1.
XP_005214785.1. XM_005214728.1.
UniGeneiBt.9056.

Genome annotation databases

EnsembliENSBTAT00000004559; ENSBTAP00000004559; ENSBTAG00000003504.
GeneIDi525059.
KEGGibta:525059.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BT020647 mRNA. Translation: AAX08664.1 .
BC109713 mRNA. Translation: AAI09714.1 .
RefSeqi NP_001015630.1. NM_001015630.1.
XP_005214785.1. XM_005214728.1.
UniGenei Bt.9056.

3D structure databases

ProteinModelPortali Q5EAC2.
SMRi Q5EAC2. Positions 3-474.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PaxDbi Q5EAC2.
PRIDEi Q5EAC2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSBTAT00000004559 ; ENSBTAP00000004559 ; ENSBTAG00000003504 .
GeneIDi 525059.
KEGGi bta:525059.

Organism-specific databases

CTDi 2937.

Phylogenomic databases

eggNOGi NOG329040.
GeneTreei ENSGT00390000013764.
HOGENOMi HOG000172641.
HOVERGENi HBG002458.
InParanoidi Q5EAC2.
KOi K01920.
OrthoDBi EOG757CXD.
TreeFami TF105187.

Enzyme and pathway databases

UniPathwayi UPA00142 ; UER00210 .
Reactomei REACT_227316. Glutathione synthesis and recycling.

Miscellaneous databases

NextBioi 20874086.

Family and domain databases

Gene3Di 1.10.1080.10. 2 hits.
3.30.1490.50. 1 hit.
3.30.1490.80. 2 hits.
3.40.50.1760. 1 hit.
InterProi IPR004887. Glutathione_synth_subst-bd_euk.
IPR014042. Glutathione_synthase_a-hlx_euk.
IPR014709. Glutathione_synthase_dom.
IPR005615. Glutathione_synthase_euk.
IPR014049. Glutathione_synthase_N_euk.
IPR016185. PreATP-grasp_dom.
[Graphical view ]
PANTHERi PTHR11130. PTHR11130. 1 hit.
Pfami PF03917. GSH_synth_ATP. 1 hit.
PF03199. GSH_synthase. 1 hit.
[Graphical view ]
PIRSFi PIRSF001558. GSHase. 1 hit.
SUPFAMi SSF52440. SSF52440. 1 hit.
TIGRFAMsi TIGR01986. glut_syn_euk. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Crossbred X Angus.
    Tissue: Liver.

Entry informationi

Entry nameiGSHB_BOVIN
AccessioniPrimary (citable) accession number: Q5EAC2
Secondary accession number(s): Q2TBS8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: March 15, 2005
Last modified: September 3, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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