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Protein

Glutathione synthetase

Gene

GSS

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Pathwayi: glutathione biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes glutathione from L-cysteine and L-glutamate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamate--cysteine ligase regulatory subunit (GCLM)
  2. Glutathione synthetase (GSS), Glutathione synthetase (GSS)
This subpathway is part of the pathway glutathione biosynthesis, which is itself part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes glutathione from L-cysteine and L-glutamate, the pathway glutathione biosynthesis and in Sulfur metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei125SubstrateBy similarity1
Metal bindingi144MagnesiumBy similarity1
Binding sitei144ATPBy similarity1
Metal bindingi146MagnesiumBy similarity1
Binding sitei220SubstrateBy similarity1
Binding sitei305ATPBy similarity1
Metal bindingi368MagnesiumBy similarity1
Binding sitei375ATPBy similarity1
Binding sitei425ATPBy similarity1
Binding sitei450SubstrateBy similarity1
Binding sitei452ATPBy similarity1
Binding sitei458ATP; via carbonyl oxygenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi364 – 373ATPBy similarity10
Nucleotide bindingi398 – 401ATPBy similarity4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Glutathione biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00142; UER00210.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione synthetase (EC:6.3.2.3)
Short name:
GSH synthetase
Short name:
GSH-S
Alternative name(s):
Glutathione synthase
Gene namesi
Name:GSS
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002475492 – 474Glutathione synthetaseAdd BLAST473

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei415PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ5EAC2.
PeptideAtlasiQ5EAC2.
PRIDEiQ5EAC2.

Expressioni

Gene expression databases

BgeeiENSBTAG00000003504.

Interactioni

Subunit structurei

Homodimer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000004559.

Structurei

3D structure databases

ProteinModelPortaliQ5EAC2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni148 – 151Substrate bindingBy similarity4
Regioni214 – 216Substrate bindingBy similarity3
Regioni267 – 270Substrate bindingBy similarity4
Regioni461 – 462Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the eukaryotic GSH synthase family.Curated

Phylogenomic databases

eggNOGiKOG0021. Eukaryota.
ENOG410XPHH. LUCA.
HOGENOMiHOG000172641.
HOVERGENiHBG002458.
InParanoidiQ5EAC2.
KOiK01920.
TreeFamiTF105187.

Family and domain databases

Gene3Di1.10.1080.10. 2 hits.
3.30.1490.50. 1 hit.
3.30.1490.80. 2 hits.
3.40.50.1760. 1 hit.
InterProiIPR004887. Glutathione_synth_subst-bd_euk.
IPR014042. Glutathione_synthase_a-hlx_euk.
IPR014709. Glutathione_synthase_dom.
IPR005615. Glutathione_synthase_euk.
IPR014049. Glutathione_synthase_N_euk.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERiPTHR11130. PTHR11130. 1 hit.
PfamiPF03917. GSH_synth_ATP. 1 hit.
PF03199. GSH_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF001558. GSHase. 1 hit.
SUPFAMiSSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01986. glut_syn_euk. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5EAC2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATGWGSLLQ DEQQLEELAR QAVDRALAEG VLLRTSQAPS SSHVVSYAPF
60 70 80 90 100
TLFPSPVPSA LLEQAYAVQA DFNLLVDAVS QNAVFLEQTL SSTIKRDSFT
110 120 130 140 150
ARLFDIHKQV LKEGIAQTVF LGLNRSDYMF QCNPDGSAAL KQIEINTVSA
160 170 180 190 200
SFGGLASRTP AVHRHVLSVL GKTKEAAKIL SNNPSKGLAM GIAKAWELYG
210 220 230 240 250
SANAQVLLIA QEKERNIFDQ RAIENELLAR NIHVIRRKFE DVSEKGSLDQ
260 270 280 290 300
DRRLFMDGQE IAVVYFRDGY MPGHYSLQNW EARLLLERSC AVKCPDIATQ
310 320 330 340 350
LAGTKKVQQE LSRVGVLESF LPGQPEAVAR LRATFAGLYS LDLGEEGDQA
360 370 380 390 400
ITKAIAAPSC FVLKPQREGG GNNLYGEEMV QALERLKDSE ERASYILMEK
410 420 430 440 450
IEPEPFRNCL LRPGSPARVI QCISELGIFG VYVREGKTLV MNKHVGHLLR
460 470
TKAIEHADGG VAAGVAVLDN PYPV
Length:474
Mass (Da):52,066
Last modified:March 15, 2005 - v1
Checksum:i964C08AF6A93FFF8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti238K → R in AAI09714 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT020647 mRNA. Translation: AAX08664.1.
BC109713 mRNA. Translation: AAI09714.1.
RefSeqiNP_001015630.1. NM_001015630.1.
XP_005214785.1. XM_005214728.3.
UniGeneiBt.9056.

Genome annotation databases

GeneIDi525059.
KEGGibta:525059.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT020647 mRNA. Translation: AAX08664.1.
BC109713 mRNA. Translation: AAI09714.1.
RefSeqiNP_001015630.1. NM_001015630.1.
XP_005214785.1. XM_005214728.3.
UniGeneiBt.9056.

3D structure databases

ProteinModelPortaliQ5EAC2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000004559.

Proteomic databases

PaxDbiQ5EAC2.
PeptideAtlasiQ5EAC2.
PRIDEiQ5EAC2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi525059.
KEGGibta:525059.

Organism-specific databases

CTDi2937.

Phylogenomic databases

eggNOGiKOG0021. Eukaryota.
ENOG410XPHH. LUCA.
HOGENOMiHOG000172641.
HOVERGENiHBG002458.
InParanoidiQ5EAC2.
KOiK01920.
TreeFamiTF105187.

Enzyme and pathway databases

UniPathwayiUPA00142; UER00210.

Gene expression databases

BgeeiENSBTAG00000003504.

Family and domain databases

Gene3Di1.10.1080.10. 2 hits.
3.30.1490.50. 1 hit.
3.30.1490.80. 2 hits.
3.40.50.1760. 1 hit.
InterProiIPR004887. Glutathione_synth_subst-bd_euk.
IPR014042. Glutathione_synthase_a-hlx_euk.
IPR014709. Glutathione_synthase_dom.
IPR005615. Glutathione_synthase_euk.
IPR014049. Glutathione_synthase_N_euk.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERiPTHR11130. PTHR11130. 1 hit.
PfamiPF03917. GSH_synth_ATP. 1 hit.
PF03199. GSH_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF001558. GSHase. 1 hit.
SUPFAMiSSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01986. glut_syn_euk. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGSHB_BOVIN
AccessioniPrimary (citable) accession number: Q5EAC2
Secondary accession number(s): Q2TBS8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: March 15, 2005
Last modified: November 30, 2016
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.