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Q5EAC2 (GSHB_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 57. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutathione synthetase

Short name=GSH synthetase
Short name=GSH-S
EC=6.3.2.3
Alternative name(s):
Glutathione synthase
Gene names
Name:GSS
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length474 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.

Cofactor

Binds 1 magnesium ion per subunit By similarity.

Pathway

Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 2/2.

Subunit structure

Homodimer By similarity.

Sequence similarities

Belongs to the eukaryotic GSH synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 474473Glutathione synthetase
PRO_0000247549

Regions

Nucleotide binding364 – 37310ATP By similarity
Nucleotide binding398 – 4014ATP By similarity
Region148 – 1514Substrate binding By similarity
Region214 – 2163Substrate binding By similarity
Region267 – 2704Substrate binding By similarity
Region461 – 4622Substrate binding By similarity

Sites

Metal binding1441Magnesium By similarity
Metal binding1461Magnesium By similarity
Metal binding3681Magnesium By similarity
Binding site1251Substrate By similarity
Binding site1441ATP By similarity
Binding site2201Substrate By similarity
Binding site3051ATP By similarity
Binding site3751ATP By similarity
Binding site4251ATP By similarity
Binding site4501Substrate By similarity
Binding site4521ATP By similarity
Binding site4581ATP; via carbonyl oxygen By similarity

Amino acid modifications

Modified residue21N-acetylalanine By similarity

Experimental info

Sequence conflict2381K → R in AAI09714. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q5EAC2 [UniParc].

Last modified March 15, 2005. Version 1.
Checksum: 964C08AF6A93FFF8

FASTA47452,066
        10         20         30         40         50         60 
MATGWGSLLQ DEQQLEELAR QAVDRALAEG VLLRTSQAPS SSHVVSYAPF TLFPSPVPSA 

        70         80         90        100        110        120 
LLEQAYAVQA DFNLLVDAVS QNAVFLEQTL SSTIKRDSFT ARLFDIHKQV LKEGIAQTVF 

       130        140        150        160        170        180 
LGLNRSDYMF QCNPDGSAAL KQIEINTVSA SFGGLASRTP AVHRHVLSVL GKTKEAAKIL 

       190        200        210        220        230        240 
SNNPSKGLAM GIAKAWELYG SANAQVLLIA QEKERNIFDQ RAIENELLAR NIHVIRRKFE 

       250        260        270        280        290        300 
DVSEKGSLDQ DRRLFMDGQE IAVVYFRDGY MPGHYSLQNW EARLLLERSC AVKCPDIATQ 

       310        320        330        340        350        360 
LAGTKKVQQE LSRVGVLESF LPGQPEAVAR LRATFAGLYS LDLGEEGDQA ITKAIAAPSC 

       370        380        390        400        410        420 
FVLKPQREGG GNNLYGEEMV QALERLKDSE ERASYILMEK IEPEPFRNCL LRPGSPARVI 

       430        440        450        460        470 
QCISELGIFG VYVREGKTLV MNKHVGHLLR TKAIEHADGG VAAGVAVLDN PYPV 

« Hide

References

[1]"Characterization of 954 bovine full-CDS cDNA sequences."
Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L.
BMC Genomics 6:166-166(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[2]NIH - Mammalian Gene Collection (MGC) project
Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Crossbred X Angus.
Tissue: Liver.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BT020647 mRNA. Translation: AAX08664.1.
BC109713 mRNA. Translation: AAI09714.1.
RefSeqNP_001015630.1. NM_001015630.1.
XP_005214785.1. XM_005214728.1.
UniGeneBt.9056.

3D structure databases

ProteinModelPortalQ5EAC2.
SMRQ5EAC2. Positions 3-474.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PaxDbQ5EAC2.
PRIDEQ5EAC2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSBTAT00000004559; ENSBTAP00000004559; ENSBTAG00000003504.
GeneID525059.
KEGGbta:525059.

Organism-specific databases

CTD2937.

Phylogenomic databases

eggNOGNOG329040.
GeneTreeENSGT00390000013764.
HOGENOMHOG000172641.
HOVERGENHBG002458.
InParanoidQ5EAC2.
KOK01920.
OrthoDBEOG757CXD.
TreeFamTF105187.

Enzyme and pathway databases

UniPathwayUPA00142; UER00210.

Family and domain databases

Gene3D1.10.1080.10. 2 hits.
3.30.1490.50. 1 hit.
3.30.1490.80. 2 hits.
3.40.50.1760. 1 hit.
InterProIPR004887. Glutathione_synth_subst-bd_euk.
IPR014042. Glutathione_synthase_a-hlx_euk.
IPR014709. Glutathione_synthase_dom.
IPR005615. Glutathione_synthase_euk.
IPR014049. Glutathione_synthase_N_euk.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR11130. PTHR11130. 1 hit.
PfamPF03917. GSH_synth_ATP. 1 hit.
PF03199. GSH_synthase. 1 hit.
[Graphical view]
PIRSFPIRSF001558. GSHase. 1 hit.
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01986. glut_syn_euk. 1 hit.
ProtoNetSearch...

Other

NextBio20874086.

Entry information

Entry nameGSHB_BOVIN
AccessionPrimary (citable) accession number: Q5EAC2
Secondary accession number(s): Q2TBS8
Entry history
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: March 15, 2005
Last modified: February 19, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways