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Q5EAC2

- GSHB_BOVIN

UniProt

Q5EAC2 - GSHB_BOVIN

Protein

Glutathione synthetase

Gene

GSS

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 59 (01 Oct 2014)
      Sequence version 1 (15 Mar 2005)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.

    Cofactori

    Binds 1 magnesium ion per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei125 – 1251SubstrateBy similarity
    Metal bindingi144 – 1441MagnesiumBy similarity
    Binding sitei144 – 1441ATPBy similarity
    Metal bindingi146 – 1461MagnesiumBy similarity
    Binding sitei220 – 2201SubstrateBy similarity
    Binding sitei305 – 3051ATPBy similarity
    Metal bindingi368 – 3681MagnesiumBy similarity
    Binding sitei375 – 3751ATPBy similarity
    Binding sitei425 – 4251ATPBy similarity
    Binding sitei450 – 4501SubstrateBy similarity
    Binding sitei452 – 4521ATPBy similarity
    Binding sitei458 – 4581ATP; via carbonyl oxygenBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi364 – 37310ATPBy similarity
    Nucleotide bindingi398 – 4014ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB
    2. glutathione binding Source: UniProtKB
    3. glutathione synthase activity Source: UniProtKB-EC
    4. magnesium ion binding Source: UniProtKB
    5. protein homodimerization activity Source: UniProtKB

    GO - Biological processi

    1. response to cadmium ion Source: Ensembl

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Glutathione biosynthesis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_227316. Glutathione synthesis and recycling.
    UniPathwayiUPA00142; UER00210.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutathione synthetase (EC:6.3.2.3)
    Short name:
    GSH synthetase
    Short name:
    GSH-S
    Alternative name(s):
    Glutathione synthase
    Gene namesi
    Name:GSS
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 13

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 474473Glutathione synthetasePRO_0000247549Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PaxDbiQ5EAC2.
    PRIDEiQ5EAC2.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ5EAC2.
    SMRiQ5EAC2. Positions 3-474.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni148 – 1514Substrate bindingBy similarity
    Regioni214 – 2163Substrate bindingBy similarity
    Regioni267 – 2704Substrate bindingBy similarity
    Regioni461 – 4622Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the eukaryotic GSH synthase family.Curated

    Phylogenomic databases

    eggNOGiNOG329040.
    GeneTreeiENSGT00390000013764.
    HOGENOMiHOG000172641.
    HOVERGENiHBG002458.
    InParanoidiQ5EAC2.
    KOiK01920.
    OrthoDBiEOG757CXD.
    TreeFamiTF105187.

    Family and domain databases

    Gene3Di1.10.1080.10. 2 hits.
    3.30.1490.50. 1 hit.
    3.30.1490.80. 2 hits.
    3.40.50.1760. 1 hit.
    InterProiIPR004887. Glutathione_synth_subst-bd_euk.
    IPR014042. Glutathione_synthase_a-hlx_euk.
    IPR014709. Glutathione_synthase_dom.
    IPR005615. Glutathione_synthase_euk.
    IPR014049. Glutathione_synthase_N_euk.
    IPR016185. PreATP-grasp_dom.
    [Graphical view]
    PANTHERiPTHR11130. PTHR11130. 1 hit.
    PfamiPF03917. GSH_synth_ATP. 1 hit.
    PF03199. GSH_synthase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001558. GSHase. 1 hit.
    SUPFAMiSSF52440. SSF52440. 1 hit.
    TIGRFAMsiTIGR01986. glut_syn_euk. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q5EAC2-1 [UniParc]FASTAAdd to Basket

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    MATGWGSLLQ DEQQLEELAR QAVDRALAEG VLLRTSQAPS SSHVVSYAPF    50
    TLFPSPVPSA LLEQAYAVQA DFNLLVDAVS QNAVFLEQTL SSTIKRDSFT 100
    ARLFDIHKQV LKEGIAQTVF LGLNRSDYMF QCNPDGSAAL KQIEINTVSA 150
    SFGGLASRTP AVHRHVLSVL GKTKEAAKIL SNNPSKGLAM GIAKAWELYG 200
    SANAQVLLIA QEKERNIFDQ RAIENELLAR NIHVIRRKFE DVSEKGSLDQ 250
    DRRLFMDGQE IAVVYFRDGY MPGHYSLQNW EARLLLERSC AVKCPDIATQ 300
    LAGTKKVQQE LSRVGVLESF LPGQPEAVAR LRATFAGLYS LDLGEEGDQA 350
    ITKAIAAPSC FVLKPQREGG GNNLYGEEMV QALERLKDSE ERASYILMEK 400
    IEPEPFRNCL LRPGSPARVI QCISELGIFG VYVREGKTLV MNKHVGHLLR 450
    TKAIEHADGG VAAGVAVLDN PYPV 474
    Length:474
    Mass (Da):52,066
    Last modified:March 15, 2005 - v1
    Checksum:i964C08AF6A93FFF8
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti238 – 2381K → R in AAI09714. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BT020647 mRNA. Translation: AAX08664.1.
    BC109713 mRNA. Translation: AAI09714.1.
    RefSeqiNP_001015630.1. NM_001015630.1.
    XP_005214785.1. XM_005214728.1.
    UniGeneiBt.9056.

    Genome annotation databases

    EnsembliENSBTAT00000004559; ENSBTAP00000004559; ENSBTAG00000003504.
    GeneIDi525059.
    KEGGibta:525059.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BT020647 mRNA. Translation: AAX08664.1 .
    BC109713 mRNA. Translation: AAI09714.1 .
    RefSeqi NP_001015630.1. NM_001015630.1.
    XP_005214785.1. XM_005214728.1.
    UniGenei Bt.9056.

    3D structure databases

    ProteinModelPortali Q5EAC2.
    SMRi Q5EAC2. Positions 3-474.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PaxDbi Q5EAC2.
    PRIDEi Q5EAC2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000004559 ; ENSBTAP00000004559 ; ENSBTAG00000003504 .
    GeneIDi 525059.
    KEGGi bta:525059.

    Organism-specific databases

    CTDi 2937.

    Phylogenomic databases

    eggNOGi NOG329040.
    GeneTreei ENSGT00390000013764.
    HOGENOMi HOG000172641.
    HOVERGENi HBG002458.
    InParanoidi Q5EAC2.
    KOi K01920.
    OrthoDBi EOG757CXD.
    TreeFami TF105187.

    Enzyme and pathway databases

    UniPathwayi UPA00142 ; UER00210 .
    Reactomei REACT_227316. Glutathione synthesis and recycling.

    Miscellaneous databases

    NextBioi 20874086.

    Family and domain databases

    Gene3Di 1.10.1080.10. 2 hits.
    3.30.1490.50. 1 hit.
    3.30.1490.80. 2 hits.
    3.40.50.1760. 1 hit.
    InterProi IPR004887. Glutathione_synth_subst-bd_euk.
    IPR014042. Glutathione_synthase_a-hlx_euk.
    IPR014709. Glutathione_synthase_dom.
    IPR005615. Glutathione_synthase_euk.
    IPR014049. Glutathione_synthase_N_euk.
    IPR016185. PreATP-grasp_dom.
    [Graphical view ]
    PANTHERi PTHR11130. PTHR11130. 1 hit.
    Pfami PF03917. GSH_synth_ATP. 1 hit.
    PF03199. GSH_synthase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001558. GSHase. 1 hit.
    SUPFAMi SSF52440. SSF52440. 1 hit.
    TIGRFAMsi TIGR01986. glut_syn_euk. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    2. NIH - Mammalian Gene Collection (MGC) project
      Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Crossbred X Angus.
      Tissue: Liver.

    Entry informationi

    Entry nameiGSHB_BOVIN
    AccessioniPrimary (citable) accession number: Q5EAC2
    Secondary accession number(s): Q2TBS8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 25, 2006
    Last sequence update: March 15, 2005
    Last modified: October 1, 2014
    This is version 59 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3