Reviewed, UniProtKB/Swiss-Prot Q5EAB6 (PMGT1_BOVIN)

Names and origin

Protein names

Recommended name:
    Protein O-linked-mannose beta-1,2-N-acetylglucosaminyltransferase 1
      Short name=POMGnT1
    EC=2.4.1.-

Gene names

Name:

POMGNT1

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	660 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Participates in O-mannosyl glycosylation. May be responsible for the synthesis of the GlcNAc(beta1-2)Man(alpha1-)O-Ser/Thr moiety on alpha-dystroglycan and other O-mannosylated proteins. Is specific for alpha linked terminal mannose By similarity.
Catalytic activity	UDP-N-acetyl-D-glucosamine + Man-R = N-acetyl-D-glucosamine-beta-1,2-Man-R + UDP.
Cofactor	Manganese By similarity.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein Potential.
Sequence similarities	Belongs to the glycosyltransferase 13 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Ligand	Manganese
Molecular function	Glycosyltransferase Transferase
PTM	Isopeptide bond Ubl conjugation
Gene Ontology (GO)
Biological process	protein amino acid N-linked glycosylation Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi membrane Inferred from electronic annotation. Source: InterPro integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	alpha-1,3-mannosylglycoprotein 2-beta-N-acetylglucosaminyltransferase activity Inferred from electronic annotation. Source: InterPro manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 660	660	Protein O-linked-mannose beta-1,2-N-acetylglucosaminyltransferase 1		PRO_0000191389
Regions
	Topological domain	1 – 37	37	Cytoplasmic Potential
	Transmembrane	38 – 58	21	Signal-anchor for type II membrane protein Potential
	Topological domain	59 – 660	602	Lumenal Potential
	Compositional bias	263 – 267	5	Poly-Arg
Amino acid modifications
	Cross-link	537		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity
	Cross-link	538		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5EAB6-1 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: B40DDF17F4ECFC3A	FASTA	660	75,077
	10 20 30 40 50 60 MDDWKPSPLI KPFGARKKRS WYLTWKYKLT NQRALRRFCQ TGAVLFLLVT VIVNIKLILD 70 80 90 100 110 120 TRRAISEASE DLEPEQDYDE ALGRLEPPRR IGSGPRRVLD VEVYSSRSKV YVAVDGTTVL 130 140 150 160 170 180 EDEAQEQGRG IHVIVLNQAT GHVMAKRVFD TYSPHEDEAM VLFLNMVAPG RVLICTVKDE 190 200 210 220 230 240 GSFHLKDTAK ALLRSLGSQA GPALGWRDTW AFVGRKGGPV LGEKHSKSPA LSSWGDPVLL 250 260 270 280 290 300 KTDVPLSSAE EAECHWADTE LNRRRRRFCS KVEGYGSVCS CKDPTPIEFS PDPLPDNKVL 310 320 330 340 350 360 NVPVAVIAGN RPNYLYRMLR SLLSAQGVSP QMITVFIDGY YEEPMDVVAL FGLRGIQHTP 370 380 390 400 410 420 ISIKNARVSQ HYKASLTATF NLFPEAKFAV VLEEDLDIAV DFFSFLSQSI HLLEEDDSLY 430 440 450 460 470 480 CISAWNDQGY EHTAEDPALL YRVETMPGLG WVLRKSLYKE ELEPKWPTPE KLWDWDMWMR 490 500 510 520 530 540 MPEQRRGREC IIPDVSRSYH FGIVGLNMNG YFHEAYFKKH KFNTVPGVQL RNVDSLKKDA 550 560 570 580 590 600 YEVEVHRLLS EAEVLDHSKN PCEDSFLPDT EGHTYVAFIR MEKDDDFTTW TQLAKCLRIW 610 620 630 640 650 660 DLDVRGNHRG LWRLFRKKNH FLVVGVPASP YSMKKPPSIT PIFLESPPKE EGGPGAAEQT

References

[1]	"Characterization of 954 bovine full-CDS cDNA sequences." Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L. BMC Genomics 6:166-166(2005) [PubMed: 16305752] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BT020653 mRNA. Translation: AAX08670.1.
IPI	IPI00703553.
UniGene	Bt.11937
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q5EAB6.
Protein family/group databases
CAZy	GT13. Glycosyltransferase Family 13.
Genome annotation databases
Ensembl	ENSBTAT00000017718; ENSBTAP00000017718; ENSBTAG00000013322; Bos taurus. [Genome view] ENSBTAT00000043096; ENSBTAP00000040690; ENSBTAG00000013322; Bos taurus. [Genome view]
Phylogenomic databases
HOVERGEN	Q5EAB6.
Family and domain databases
InterPro	IPR004139. Glyco_trans_13. [Graphical view]
PANTHER	PTHR10468. Glyco_trans_13. 1 hit.
Pfam	PF03071. GNT-I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PMGT1_BOVIN

Accession

Primary (citable) accession number: Q5EAB6

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2005
Last sequence update:	March 15, 2005
Last modified:	November 24, 2009
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)