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Reviewed, UniProtKB/Swiss-Prot Q5EAB2 (CDK9_BOVIN)

Last modified September 22, 2009. Version 49. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Cell division protein kinase 9
    EC=2.7.11.22
    EC=2.7.11.23
Alternative name(s):
    Cyclin-dependent kinase 9
Gene names
Name: CDK9
OrganismBos taurus (Bovine)
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length372 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at transcript level.

General annotation (Comments)

Function

Member of the cyclin-dependent kinase pair (CDK9/cyclin-T) complex, also called positive transcription elongation factor b (P-TEFb), which facilitates the transition from abortive to production elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II), SUPT5H and RDBP. The CDK9/cyclin-K complex has also a kinase activity toward CTD of RNAP II and can substitute for P-TEFb in vitro By similarity.

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

Subunit structure

Associates with CCNT1/cyclin-T1 to form P-TEFb. P-TEFb forms a complex with AFF4/AF5Q31. Also associates with CCNK/cyclin-K. Component of a complex which is at least composed of HTATSF1/Tat-SF1, P-TEFb complex, RNA pol II, SUPT5H, and NCL/nucleolin. Component of the 7SK snRNP complex at least composed of P-TEFb (composed of CDK9 and CCNT1/cyclin-T1), HEXIM1, HEXIM2, BCDIN3, SART3 proteins and 7SK and U6 snRNAs By similarity.

Subcellular location

Nucleus By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

Contains 1 protein kinase domain.

Sequence caution

The sequence AAX46729.1 differs from that shown. Reason: Frameshift at position 231.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 372372Cell division protein kinase 9
PRO_0000085799

Regions

Domain19 – 315297Protein kinase
Nucleotide binding25 – 339ATP By similarity

Sites

Active site1491Proton acceptor By similarity
Binding site481ATP By similarity

Amino acid modifications

Modified residue1861Phosphothreonine By similarity
Modified residue3471Phosphoserine By similarity
Modified residue3531Phosphoserine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5EAB2-1 [UniParc].

Last modified March 15, 2005. Version 1.
Checksum: 50A26ECAC9A36CAD

FASTA37242,748
        10         20         30         40         50         60 
MAKQYDSVEC PFCDEVTKYE KLAKIGQGTF GEVFKAKHRK TGQKVALKKV LMENEKEGFP 

        70         80         90        100        110        120 
ITALREIKIL QLLKHENVVN LIEICRTKAS PYNRCKGSIY LVFDFCEHDL AGLLSNVLVK 

       130        140        150        160        170        180 
FTLSEIKRVM QMLLNGLYYI HRNKILHRDM KAANVLITRD GVLKLADFGL ARAFSLAKNS 

       190        200        210        220        230        240 
QPNRYTNRVV TLWYRPPELL LGERDYGPPI DLWGAGCIMA EMWTRSPIMQ GNTEQHQLAL 

       250        260        270        280        290        300 
ISQLCGSITP EVWPNVDKYE LFEKVELVKG QKRKVKDRLK AYVRDPYALD LIDKLLVLDP 

       310        320        330        340        350        360 
AQRIDSDDAL NHDFFWSDPM PSDLKGMLST HLTSMFEYLA PPRRKGSQIT QQSTNQSRNP 

       370 
ATTNQTEFER VF 

« Hide

References

[1]"Characterization of 954 bovine full-CDS cDNA sequences."
Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L.
BMC Genomics 6:166-166(2005) [PubMed: 16305752] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[2]NIH - Mammalian Gene Collection (MGC) project
Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Hereford.
Tissue: Thalamus.

Cross-references

Sequence databases

BT020657 mRNA. Translation: AAX08674.1.
BT021882 mRNA. Translation: AAX46729.1. Frameshift.
BC118194 mRNA. Translation: AAI18195.1.
IPIIPI00711058.
RefSeqNP_001014935.2.
UniGeneBt.1465

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRINGQ5EAB2.

Genome annotation databases

EnsemblENSBTAT00000006162; ENSBTAP00000006162; ENSBTAG00000004695; Bos taurus. [Genome view]
GeneID520580.
KEGGbta:520580.

Phylogenomic databases

HOVERGENQ5EAB2.

Enzyme and pathway databases

BRENDA2.7.11.22. 251.
2.7.11.23. 251.

Family and domain databases

InterProIPR000719. Prot_kinase_core.
IPR017441. Protein_kinase_ATP_BS.
IPR017442. Se/Thr_pkinase-rel.
IPR008271. Ser_thr_pkin_AS.
IPR002290. Ser_thr_pkinase.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
ProDomPD000001. Prot_kinase. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCDK9_BOVIN
AccessionPrimary (citable) accession number: Q5EAB2
Secondary accession number(s): Q148K6, Q58CR5
Entry history
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: March 15, 2005
Last modified: September 22, 2009
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents