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Protein

Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic

Gene

GPD1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

sn-glycerol 3-phosphate + NAD+ = glycerone phosphate + NADH.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei41 – 411NADBy similarity
Binding sitei97 – 971NADBy similarity
Binding sitei120 – 1201SubstrateBy similarity
Binding sitei153 – 1531NAD; via amide nitrogenBy similarity
Active sitei204 – 2041Proton acceptorSequence Analysis
Binding sitei269 – 2691NADBy similarity
Binding sitei296 – 2961NAD; via amide nitrogenBy similarity
Binding sitei298 – 2981NADBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 156NADBy similarity

GO - Molecular functioni

  1. glycerol-3-phosphate dehydrogenase [NAD+] activity Source: UniProtKB-EC
  2. glycerol-3-phosphate dehydrogenase activity Source: AgBase
  3. NAD binding Source: InterPro

GO - Biological processi

  1. carbohydrate metabolic process Source: AgBase
  2. cellular response to cAMP Source: Ensembl
  3. cellular response to tumor necrosis factor Source: Ensembl
  4. gluconeogenesis Source: AgBase
  5. glycerol-3-phosphate catabolic process Source: InterPro
  6. glycerol-3-phosphate metabolic process Source: AgBase
  7. glycerophosphate shuttle Source: Ensembl
  8. positive regulation of glycolytic process Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Enzyme and pathway databases

ReactomeiREACT_209849. Triglyceride Biosynthesis.
REACT_211382. Synthesis of PA.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic (EC:1.1.1.8)
Short name:
GPD-C
Short name:
GPDH-C
Gene namesi
Name:GPD1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome 5

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytosol Source: AgBase
  2. extracellular vesicular exosome Source: Ensembl
  3. glycerol-3-phosphate dehydrogenase complex Source: InterPro
  4. mitochondrion Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 349349Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmicPRO_0000262289Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei289 – 2891N6-succinyllysineBy similarity

Proteomic databases

PaxDbiQ5EA88.

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ5EA88.
SMRiQ5EA88. Positions 4-349.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni269 – 2702Substrate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0240.
GeneTreeiENSGT00390000003114.
HOGENOMiHOG000246855.
HOVERGENiHBG003669.
InParanoidiQ5EA88.
KOiK00006.
OMAiEVANDNF.
OrthoDBiEOG7ZKSBS.
TreeFamiTF300836.

Family and domain databases

Gene3Di1.10.1040.10. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR013328. DH_multihelical.
IPR006168. G3P_DH_NAD-dep.
IPR006109. G3P_DH_NAD-dep_C.
IPR017751. G3P_DH_NAD-dep_euk.
IPR011128. G3P_DH_NAD-dep_N.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11728. PTHR11728. 1 hit.
PfamiPF07479. NAD_Gly3P_dh_C. 1 hit.
PF01210. NAD_Gly3P_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000114. Glycerol-3-P_dh. 1 hit.
PRINTSiPR00077. GPDHDRGNASE.
SUPFAMiSSF48179. SSF48179. 1 hit.
TIGRFAMsiTIGR03376. glycerol3P_DH. 1 hit.
PROSITEiPS00957. NAD_G3PDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5EA88-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTGKKVCIVG SGNWGSAIAK IVGGNAAQLA HFDPRVTMWV FEEDIGGRKL
60 70 80 90 100
TEIINTQHEN VKYLPGHKLP PNVVAVPDVV QAAADADILI FVVPHQFIGK
110 120 130 140 150
ICDQLKGHLK ADTIGVSLIK GVDEGPKGLK LISEVIGERL GIPMSVLMGA
160 170 180 190 200
NIANEVADEK FCETTIGSKN QAHGQLLKEL MQTPNFRITV VQEVDTVEIC
210 220 230 240 250
GALKNIVAVG AGFCDGLGFG DNTKAAVIRL GLMEMIAFAK LFCSGSVSSA
260 270 280 290 300
TFLESCGVAD LITTCYGGRN RKVAEAFART GKSIEQLEKE MLNGQKLQGP
310 320 330 340
QTARELHSIL QHKGMVDKFP LFTAVYKVCY ENQPVGEFIH CLQNHPEHV
Length:349
Mass (Da):37,648
Last modified:January 23, 2007 - v3
Checksum:i59E7DFADE8FC1A80
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti122 – 1221V → L in AAX08698 (PubMed:16305752).Curated
Sequence conflicti313 – 3131K → R in AAX08698 (PubMed:16305752).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT020681 mRNA. Translation: AAX08698.1.
BC105513 mRNA. Translation: AAI05514.1.
RefSeqiNP_001030431.1. NM_001035354.1.
UniGeneiBt.5002.

Genome annotation databases

EnsembliENSBTAT00000021683; ENSBTAP00000021683; ENSBTAG00000016296.
GeneIDi525042.
KEGGibta:525042.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT020681 mRNA. Translation: AAX08698.1.
BC105513 mRNA. Translation: AAI05514.1.
RefSeqiNP_001030431.1. NM_001035354.1.
UniGeneiBt.5002.

3D structure databases

ProteinModelPortaliQ5EA88.
SMRiQ5EA88. Positions 4-349.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PaxDbiQ5EA88.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000021683; ENSBTAP00000021683; ENSBTAG00000016296.
GeneIDi525042.
KEGGibta:525042.

Organism-specific databases

CTDi2819.

Phylogenomic databases

eggNOGiCOG0240.
GeneTreeiENSGT00390000003114.
HOGENOMiHOG000246855.
HOVERGENiHBG003669.
InParanoidiQ5EA88.
KOiK00006.
OMAiEVANDNF.
OrthoDBiEOG7ZKSBS.
TreeFamiTF300836.

Enzyme and pathway databases

ReactomeiREACT_209849. Triglyceride Biosynthesis.
REACT_211382. Synthesis of PA.

Miscellaneous databases

NextBioi20874083.

Family and domain databases

Gene3Di1.10.1040.10. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR013328. DH_multihelical.
IPR006168. G3P_DH_NAD-dep.
IPR006109. G3P_DH_NAD-dep_C.
IPR017751. G3P_DH_NAD-dep_euk.
IPR011128. G3P_DH_NAD-dep_N.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11728. PTHR11728. 1 hit.
PfamiPF07479. NAD_Gly3P_dh_C. 1 hit.
PF01210. NAD_Gly3P_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000114. Glycerol-3-P_dh. 1 hit.
PRINTSiPR00077. GPDHDRGNASE.
SUPFAMiSSF48179. SSF48179. 1 hit.
TIGRFAMsiTIGR03376. glycerol3P_DH. 1 hit.
PROSITEiPS00957. NAD_G3PDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 7-349.
    Strain: Hereford.
    Tissue: Ascending colon.

Entry informationi

Entry nameiGPDA_BOVIN
AccessioniPrimary (citable) accession number: Q5EA88
Secondary accession number(s): Q2HJE6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: January 23, 2007
Last modified: March 4, 2015
This is version 78 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.