Reviewed, UniProtKB/Swiss-Prot Q5EA88 (GPDA_BOVIN)

Names and origin

Protein names

Recommended name:
    Glycerol-3-phosphate dehydrogenase [NAD+], cytoplasmic
      Short name=GPDH-C
      Short name=GPD-C
    EC=1.1.1.8

Gene names

Name:

GPD1

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	349 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	sn-glycerol 3-phosphate + NAD(+) = glycerone phosphate + NADH.
Subunit structure	Homodimer By similarity.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	gluconeogenesis Inferred from sequence or structural similarity. Source: AgBase glycerol-3-phosphate catabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytosol Inferred from sequence or structural similarity. Source: AgBase glycerol-3-phosphate dehydrogenase complex Inferred from electronic annotation. Source: InterPro
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro glycerol-3-phosphate dehydrogenase (NAD+) activity Inferred from electronic annotation. Source: InterPro glycerol-3-phosphate dehydrogenase activity Inferred from sequence or structural similarity. Source: AgBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 349	348	Glycerol-3-phosphate dehydrogenase [NAD+], cytoplasmic		PRO_0000262289
Regions
	Nucleotide binding	10 – 15	6	NAD By similarity
	Region	269 – 270	2	Substrate binding By similarity
Sites
	Active site	204	1	Proton acceptor Potential
	Binding site	41	1	NAD By similarity
	Binding site	97	1	NAD By similarity
	Binding site	120	1	Substrate By similarity
	Binding site	153	1	NAD; via amide nitrogen By similarity
	Binding site	269	1	NAD By similarity
	Binding site	296	1	NAD; via amide nitrogen By similarity
	Binding site	298	1	NAD By similarity
Experimental info
	Sequence conflict	122	1	V → L in AAX08698. Ref.1
	Sequence conflict	313	1	K → R in AAX08698. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q5EA88-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 59E7DFADE8FC1A80	FASTA	349	37,648
	10 20 30 40 50 60 MTGKKVCIVG SGNWGSAIAK IVGGNAAQLA HFDPRVTMWV FEEDIGGRKL TEIINTQHEN 70 80 90 100 110 120 VKYLPGHKLP PNVVAVPDVV QAAADADILI FVVPHQFIGK ICDQLKGHLK ADTIGVSLIK 130 140 150 160 170 180 GVDEGPKGLK LISEVIGERL GIPMSVLMGA NIANEVADEK FCETTIGSKN QAHGQLLKEL 190 200 210 220 230 240 MQTPNFRITV VQEVDTVEIC GALKNIVAVG AGFCDGLGFG DNTKAAVIRL GLMEMIAFAK 250 260 270 280 290 300 LFCSGSVSSA TFLESCGVAD LITTCYGGRN RKVAEAFART GKSIEQLEKE MLNGQKLQGP 310 320 330 340 QTARELHSIL QHKGMVDKFP LFTAVYKVCY ENQPVGEFIH CLQNHPEHV

References

[1]	"Characterization of 954 bovine full-CDS cDNA sequences." Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L. BMC Genomics 6:166-166(2005) [PubMed: 16305752] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[2]	NIH - Mammalian Gene Collection (MGC) project Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 7-349. Strain: Hereford. Tissue: Ascending colon.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BT020681 mRNA. Translation: AAX08698.1. BC105513 mRNA. Translation: AAI05514.1.
RefSeq	NP_001030431.1.
UniGene	Bt.5002
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSBTAG00000016296. Bos taurus. [Contig view]
GeneID	525042.
KEGG	bta:525042.
Phylogenomic databases
HOVERGEN	Q5EA88.
Family and domain databases
InterPro	IPR013328. DHase_multihelical. IPR016040. NAD(P)-bd. IPR017751. NAD-dep_Gly3P_DH_euk. IPR006168. NAD-dep_Gly3P_DHase. IPR011128. NAD-dep_Gly3P_DHase_N. IPR006109. NAD_Gly3P_DHase_C. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. G3DSA:1.10.1040.10. Opine_DH. 1 hit.
PANTHER	PTHR11728. NAD_Gly3P_DH. 1 hit.
Pfam	PF07479. NAD_Gly3P_dh_C. 1 hit. PF01210. NAD_Gly3P_dh_N. 1 hit. [Graphical view]
PIRSF	PIRSF000114. Glycerol-3-P_dh. 1 hit.
PRINTS	PR00077. GPDHDRGNASE.
ProDom	PD001278. NAD_Gly3P_C. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00957. NAD_G3PDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GPDA_BOVIN

Accession

Primary (citable) accession number: Q5EA88
Secondary accession number(s): Q2HJE6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	January 23, 2007
Last modified:	November 25, 2008
This is version 27 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)