GPD1

Functionⁱ

Catalytic activityⁱ

sn-glycerol 3-phosphate + NAD⁺ = glycerone phosphate + NADH.

Sites

Feature key	Position(s)	DescriptionActions	Length
Binding siteⁱ	41	NADBy similarity	1
Binding siteⁱ	97	NADBy similarity	1
Binding siteⁱ	120	SubstrateBy similarity	1
Binding siteⁱ	153	NAD; via amide nitrogenBy similarity	1
Active siteⁱ	204	Proton acceptorSequence analysis	1
Binding siteⁱ	269	NADBy similarity	1
Binding siteⁱ	296	NAD; via amide nitrogenBy similarity	1
Binding siteⁱ	298	NADBy similarity	1

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Nucleotide bindingⁱ	10 – 15	NADBy similarity		6

GO - Molecular functionⁱ

glycerol-3-phosphate dehydrogenase [NAD+] activity Source: UniProtKB-EC
glycerol-3-phosphate dehydrogenase activity Source: AgBase
NAD binding Source: InterPro
protein homodimerization activity Source: InterPro

Complete GO annotation...

GO - Biological processⁱ

carbohydrate metabolic process Source: AgBase
cellular response to cAMP Source: Ensembl
cellular response to tumor necrosis factor Source: Ensembl
gluconeogenesis Source: AgBase
glycerol-3-phosphate catabolic process Source: InterPro
glycerol-3-phosphate metabolic process Source: AgBase
glycerophosphate shuttle Source: Ensembl
positive regulation of glycolytic process Source: Ensembl

Complete GO annotation...

Keywordsⁱ

Molecular function	Oxidoreductase
Ligand	NAD

Enzyme and pathway databases

Reactomeⁱ	R-BTA-1483166. Synthesis of PA. R-BTA-75109. Triglyceride Biosynthesis.

Reactomeⁱ

R-BTA-1483166. Synthesis of PA.
R-BTA-75109. Triglyceride Biosynthesis.

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic (EC:1.1.1.8) Short name: GPD-C Short name: GPDH-C
Gene namesⁱ	Name:GPD1
Organismⁱ	Bos taurus (Bovine)
Taxonomic identifierⁱ	9913 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos
Proteomesⁱ	UP000009136 Componentⁱ: Chromosome 5

Subcellular locationⁱ

Cytoplasm By similarity

GO - Cellular componentⁱ

cytosol Source: AgBase
extracellular exosome Source: Ensembl
glycerol-3-phosphate dehydrogenase complex Source: InterPro
mitochondrion Source: Ensembl

Complete GO annotation...

Keywords - Cellular componentⁱ

Cytoplasm

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000262289	1 – 349	Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmicAdd BLAST		349

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Modified residueⁱ	289	N6-succinyllysineBy similarity		1
Modified residueⁱ	326	PhosphotyrosineBy similarity		1

Keywords - PTMⁱ

Phosphoprotein

Proteomic databases

PaxDbⁱ	Q5EA88.
PeptideAtlas More...PeptideAtlasⁱ	Q5EA88.
PRIDEⁱ	Q5EA88.

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSBTAG00000016296.

Bgeeⁱ

ENSBTAG00000016296.

Interactionⁱ

Subunit structureⁱ

Homodimer.By similarity

GO - Molecular functionⁱ

protein homodimerization activity Source: InterPro

Complete GO annotation...

Protein-protein interaction databases

STRINGⁱ	9913.ENSBTAP00000021683.

STRINGⁱ

9913.ENSBTAP00000021683.

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q5EA88.
SMRⁱ	Q5EA88.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	269 – 270	Substrate bindingBy similarity		2

Sequence similaritiesⁱ

Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family.Curated

Phylogenomic databases

eggNOGⁱ	KOG2711. Eukaryota. COG0240. LUCA.
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000003114.
HOGENOMⁱ	HOG000246855.
HOVERGENⁱ	HBG003669.
InParanoidⁱ	Q5EA88.
KEGG Orthology (KO) More...KOⁱ	K00006.
OMAⁱ	NFCEATI.
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0DO6.
TreeFamⁱ	TF300836.

Family and domain databases

Gene3Dⁱ	1.10.1040.10. 1 hit.
InterProⁱ	View protein in InterPro IPR008927. 6-PGluconate_DH_C-like. IPR013328. 6PGD_dom_2. IPR006168. G3P_DH_NAD-dep. IPR006109. G3P_DH_NAD-dep_C. IPR017751. G3P_DH_NAD-dep_euk. IPR011128. G3P_DH_NAD-dep_N. IPR016040. NAD(P)-bd_dom.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF07479. NAD_Gly3P_dh_C. 1 hit. PF01210. NAD_Gly3P_dh_N. 1 hit.
PIRSFⁱ	PIRSF000114. Glycerol-3-P_dh. 1 hit.
PRINTSⁱ	PR00077. GPDHDRGNASE.
SUPFAMⁱ	SSF48179. SSF48179. 1 hit. SSF51735. SSF51735. 1 hit.
TIGRFAMsⁱ	TIGR03376. glycerol3P_DH. 1 hit.
PROSITEⁱ	View protein in PROSITE PS00957. NAD_G3PDH. 1 hit.

Sequenceⁱ

Sequence statusⁱ: Complete.

Q5EA88-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MTGKKVCIVG SGNWGSAIAK IVGGNAAQLA HFDPRVTMWV FEEDIGGRKL 
        60         70         80         90        100
TEIINTQHEN VKYLPGHKLP PNVVAVPDVV QAAADADILI FVVPHQFIGK 
       110        120        130        140        150
ICDQLKGHLK ADTIGVSLIK GVDEGPKGLK LISEVIGERL GIPMSVLMGA 
       160        170        180        190        200
NIANEVADEK FCETTIGSKN QAHGQLLKEL MQTPNFRITV VQEVDTVEIC 
       210        220        230        240        250
GALKNIVAVG AGFCDGLGFG DNTKAAVIRL GLMEMIAFAK LFCSGSVSSA 
       260        270        280        290        300
TFLESCGVAD LITTCYGGRN RKVAEAFART GKSIEQLEKE MLNGQKLQGP 
       310        320        330        340 
QTARELHSIL QHKGMVDKFP LFTAVYKVCY ENQPVGEFIH CLQNHPEHV

Length:349

Mass (Da):37,648

Last modified:January 23, 2007 - v3

Checksum:ⁱ59E7DFADE8FC1A80

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	122	V → L in AAX08698 (PubMed:16305752).Curated		1
Sequence conflictⁱ	313	K → R in AAX08698 (PubMed:16305752).Curated		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	BT020681 mRNA. Translation: AAX08698.1. BC105513 mRNA. Translation: AAI05514.1.
NCBI Reference Sequences More...RefSeqⁱ	NP_001030431.1. NM_001035354.1.
UniGeneⁱ	Bt.5002.

Genome annotation databases

Ensemblⁱ	ENSBTAT00000021683; ENSBTAP00000021683; ENSBTAG00000016296.
GeneIDⁱ	525042.
KEGGⁱ	bta:525042.

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	BT020681 mRNA. Translation: AAX08698.1. BC105513 mRNA. Translation: AAI05514.1.
NCBI Reference Sequences More...RefSeqⁱ	NP_001030431.1. NM_001035354.1.
UniGeneⁱ	Bt.5002.

3D structure databases

ProteinModelPortalⁱ	Q5EA88.
SMRⁱ	Q5EA88.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

STRINGⁱ	9913.ENSBTAP00000021683.

STRINGⁱ

9913.ENSBTAP00000021683.

Proteomic databases

PaxDbⁱ	Q5EA88.
PeptideAtlas More...PeptideAtlasⁱ	Q5EA88.
PRIDEⁱ	Q5EA88.

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSBTAT00000021683; ENSBTAP00000021683; ENSBTAG00000016296.
GeneIDⁱ	525042.
KEGGⁱ	bta:525042.

Organism-specific databases

CTDⁱ	2819.

CTDⁱ

2819.

Phylogenomic databases

eggNOGⁱ	KOG2711. Eukaryota. COG0240. LUCA.
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000003114.
HOGENOMⁱ	HOG000246855.
HOVERGENⁱ	HBG003669.
InParanoidⁱ	Q5EA88.
KEGG Orthology (KO) More...KOⁱ	K00006.
OMAⁱ	NFCEATI.
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0DO6.
TreeFamⁱ	TF300836.

Enzyme and pathway databases

Reactomeⁱ	R-BTA-1483166. Synthesis of PA. R-BTA-75109. Triglyceride Biosynthesis.

Reactomeⁱ

R-BTA-1483166. Synthesis of PA.
R-BTA-75109. Triglyceride Biosynthesis.

Gene expression databases

Bgeeⁱ	ENSBTAG00000016296.

Bgeeⁱ

ENSBTAG00000016296.

Family and domain databases

Gene3Dⁱ	1.10.1040.10. 1 hit.
InterProⁱ	View protein in InterPro IPR008927. 6-PGluconate_DH_C-like. IPR013328. 6PGD_dom_2. IPR006168. G3P_DH_NAD-dep. IPR006109. G3P_DH_NAD-dep_C. IPR017751. G3P_DH_NAD-dep_euk. IPR011128. G3P_DH_NAD-dep_N. IPR016040. NAD(P)-bd_dom.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF07479. NAD_Gly3P_dh_C. 1 hit. PF01210. NAD_Gly3P_dh_N. 1 hit.
PIRSFⁱ	PIRSF000114. Glycerol-3-P_dh. 1 hit.
PRINTSⁱ	PR00077. GPDHDRGNASE.
SUPFAMⁱ	SSF48179. SSF48179. 1 hit. SSF51735. SSF51735. 1 hit.
TIGRFAMsⁱ	TIGR03376. glycerol3P_DH. 1 hit.
PROSITEⁱ	View protein in PROSITE PS00957. NAD_G3PDH. 1 hit.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	GPDA_BOVIN
Accessionⁱ	Q5EA88Primary (citable) accession number: Q5EA88 Secondary accession number(s): Q2HJE6
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
	Last sequence update:	January 23, 2007
	Last modified:	May 10, 2017
	This is version 93 of the entry and version 3 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families

Similar proteinsⁱ

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:

100%	UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%	UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%	UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q5EA88 (GPDA_BOVIN)

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Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Sites

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Proteomic databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including length and molecular weight.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Sequenceⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ