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Q5EA88

- GPDA_BOVIN

UniProt

Q5EA88 - GPDA_BOVIN

Protein

Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic

Gene

GPD1

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 75 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    sn-glycerol 3-phosphate + NAD+ = glycerone phosphate + NADH.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei41 – 411NADBy similarity
    Binding sitei97 – 971NADBy similarity
    Binding sitei120 – 1201SubstrateBy similarity
    Binding sitei153 – 1531NAD; via amide nitrogenBy similarity
    Active sitei204 – 2041Proton acceptorSequence Analysis
    Binding sitei269 – 2691NADBy similarity
    Binding sitei296 – 2961NAD; via amide nitrogenBy similarity
    Binding sitei298 – 2981NADBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi10 – 156NADBy similarity

    GO - Molecular functioni

    1. glycerol-3-phosphate dehydrogenase [NAD+] activity Source: UniProtKB-EC
    2. glycerol-3-phosphate dehydrogenase activity Source: AgBase
    3. NAD binding Source: InterPro

    GO - Biological processi

    1. carbohydrate metabolic process Source: AgBase
    2. cellular response to cAMP Source: Ensembl
    3. cellular response to tumor necrosis factor Source: Ensembl
    4. gluconeogenesis Source: AgBase
    5. glycerol-3-phosphate catabolic process Source: InterPro
    6. glycerol-3-phosphate metabolic process Source: AgBase

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    ReactomeiREACT_209849. Triglyceride Biosynthesis.
    REACT_211382. Synthesis of PA.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic (EC:1.1.1.8)
    Short name:
    GPD-C
    Short name:
    GPDH-C
    Gene namesi
    Name:GPD1
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 5

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytosol Source: AgBase
    2. glycerol-3-phosphate dehydrogenase complex Source: InterPro
    3. mitochondrion Source: Ensembl

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 349349Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmicPRO_0000262289Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei289 – 2891N6-succinyllysineBy similarity

    Proteomic databases

    PaxDbiQ5EA88.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ5EA88.
    SMRiQ5EA88. Positions 4-349.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni269 – 2702Substrate bindingBy similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0240.
    GeneTreeiENSGT00390000003114.
    HOGENOMiHOG000246855.
    HOVERGENiHBG003669.
    InParanoidiQ5EA88.
    KOiK00006.
    OMAiPVGEFIH.
    OrthoDBiEOG7ZKSBS.
    TreeFamiTF300836.

    Family and domain databases

    Gene3Di1.10.1040.10. 1 hit.
    3.40.50.720. 1 hit.
    InterProiIPR008927. 6-PGluconate_DH_C-like.
    IPR013328. DH_multihelical.
    IPR006168. G3P_DH_NAD-dep.
    IPR006109. G3P_DH_NAD-dep_C.
    IPR017751. G3P_DH_NAD-dep_euk.
    IPR011128. G3P_DH_NAD-dep_N.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PANTHERiPTHR11728. PTHR11728. 1 hit.
    PfamiPF07479. NAD_Gly3P_dh_C. 1 hit.
    PF01210. NAD_Gly3P_dh_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000114. Glycerol-3-P_dh. 1 hit.
    PRINTSiPR00077. GPDHDRGNASE.
    SUPFAMiSSF48179. SSF48179. 1 hit.
    TIGRFAMsiTIGR03376. glycerol3P_DH. 1 hit.
    PROSITEiPS00957. NAD_G3PDH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q5EA88-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTGKKVCIVG SGNWGSAIAK IVGGNAAQLA HFDPRVTMWV FEEDIGGRKL    50
    TEIINTQHEN VKYLPGHKLP PNVVAVPDVV QAAADADILI FVVPHQFIGK 100
    ICDQLKGHLK ADTIGVSLIK GVDEGPKGLK LISEVIGERL GIPMSVLMGA 150
    NIANEVADEK FCETTIGSKN QAHGQLLKEL MQTPNFRITV VQEVDTVEIC 200
    GALKNIVAVG AGFCDGLGFG DNTKAAVIRL GLMEMIAFAK LFCSGSVSSA 250
    TFLESCGVAD LITTCYGGRN RKVAEAFART GKSIEQLEKE MLNGQKLQGP 300
    QTARELHSIL QHKGMVDKFP LFTAVYKVCY ENQPVGEFIH CLQNHPEHV 349
    Length:349
    Mass (Da):37,648
    Last modified:January 23, 2007 - v3
    Checksum:i59E7DFADE8FC1A80
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti122 – 1221V → L in AAX08698. (PubMed:16305752)Curated
    Sequence conflicti313 – 3131K → R in AAX08698. (PubMed:16305752)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BT020681 mRNA. Translation: AAX08698.1.
    BC105513 mRNA. Translation: AAI05514.1.
    RefSeqiNP_001030431.1. NM_001035354.1.
    UniGeneiBt.5002.

    Genome annotation databases

    EnsembliENSBTAT00000021683; ENSBTAP00000021683; ENSBTAG00000016296.
    GeneIDi525042.
    KEGGibta:525042.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BT020681 mRNA. Translation: AAX08698.1 .
    BC105513 mRNA. Translation: AAI05514.1 .
    RefSeqi NP_001030431.1. NM_001035354.1.
    UniGenei Bt.5002.

    3D structure databases

    ProteinModelPortali Q5EA88.
    SMRi Q5EA88. Positions 4-349.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PaxDbi Q5EA88.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000021683 ; ENSBTAP00000021683 ; ENSBTAG00000016296 .
    GeneIDi 525042.
    KEGGi bta:525042.

    Organism-specific databases

    CTDi 2819.

    Phylogenomic databases

    eggNOGi COG0240.
    GeneTreei ENSGT00390000003114.
    HOGENOMi HOG000246855.
    HOVERGENi HBG003669.
    InParanoidi Q5EA88.
    KOi K00006.
    OMAi PVGEFIH.
    OrthoDBi EOG7ZKSBS.
    TreeFami TF300836.

    Enzyme and pathway databases

    Reactomei REACT_209849. Triglyceride Biosynthesis.
    REACT_211382. Synthesis of PA.

    Miscellaneous databases

    NextBioi 20874083.

    Family and domain databases

    Gene3Di 1.10.1040.10. 1 hit.
    3.40.50.720. 1 hit.
    InterProi IPR008927. 6-PGluconate_DH_C-like.
    IPR013328. DH_multihelical.
    IPR006168. G3P_DH_NAD-dep.
    IPR006109. G3P_DH_NAD-dep_C.
    IPR017751. G3P_DH_NAD-dep_euk.
    IPR011128. G3P_DH_NAD-dep_N.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    PANTHERi PTHR11728. PTHR11728. 1 hit.
    Pfami PF07479. NAD_Gly3P_dh_C. 1 hit.
    PF01210. NAD_Gly3P_dh_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000114. Glycerol-3-P_dh. 1 hit.
    PRINTSi PR00077. GPDHDRGNASE.
    SUPFAMi SSF48179. SSF48179. 1 hit.
    TIGRFAMsi TIGR03376. glycerol3P_DH. 1 hit.
    PROSITEi PS00957. NAD_G3PDH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    2. NIH - Mammalian Gene Collection (MGC) project
      Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 7-349.
      Strain: Hereford.
      Tissue: Ascending colon.

    Entry informationi

    Entry nameiGPDA_BOVIN
    AccessioniPrimary (citable) accession number: Q5EA88
    Secondary accession number(s): Q2HJE6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 28, 2006
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 75 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3