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Protein

Beta-1,4-galactosyltransferase 3

Gene

B4GALT3

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.By similarity

Catalytic activityi

UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminylglycopeptide = UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminylglycopeptide.
UDP-alpha-D-galactose + N-acetyl-D-glucosamine = UDP + N-acetyllactosamine.

Cofactori

Mn2+By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi200 – 2001ManganeseBy similarity
Binding sitei229 – 2291UDP-alpha-D-galactoseBy similarity
Binding sitei261 – 2611UDP-alpha-D-galactoseBy similarity
Metal bindingi294 – 2941Manganese; via tele nitrogenBy similarity
Binding sitei306 – 3061N-acetyl-D-glucosamineBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT7. Glycosyltransferase Family 7.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-1,4-galactosyltransferase 3 (EC:2.4.1.-)
Short name:
Beta-1,4-GalTase 3
Short name:
Beta4Gal-T3
Short name:
b4Gal-T3
Alternative name(s):
UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 3
UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 3
Including the following 3 domains:
N-acetyllactosamine synthase (EC:2.4.1.90)
Alternative name(s):
Nal synthase
Beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase (EC:2.4.1.38)
Beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase (EC:2.4.1.-)
Gene namesi
Name:B4GALT3
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1010CytoplasmicSequence analysis
Transmembranei11 – 3121Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini32 – 396365LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 396396Beta-1,4-galactosyltransferase 3PRO_0000080535Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi57 – 571N-linked (GlcNAc...)Sequence analysis
Disulfide bondi80 ↔ 122By similarity
Glycosylationi169 – 1691N-linked (GlcNAc...)Sequence analysis
Disulfide bondi193 ↔ 212By similarity
Glycosylationi340 – 3401N-linked (GlcNAc...)Sequence analysis
Glycosylationi388 – 3881N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ5EA87.

Expressioni

Gene expression databases

BgeeiENSBTAG00000021897.

Interactioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000049355.

Structurei

3D structure databases

ProteinModelPortaliQ5EA87.
SMRiQ5EA87. Positions 77-347.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni133 – 1375UDP-alpha-D-galactose bindingBy similarity
Regioni172 – 1743UDP-alpha-D-galactose bindingBy similarity
Regioni199 – 2002UDP-alpha-D-galactose bindingBy similarity
Regioni263 – 2664N-acetyl-D-glucosamine bindingBy similarity
Regioni294 – 2963UDP-alpha-D-galactose bindingBy similarity

Sequence similaritiesi

Belongs to the glycosyltransferase 7 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3916. Eukaryota.
ENOG410ZYYA. LUCA.
HOGENOMiHOG000231027.
HOVERGENiHBG058334.
InParanoidiQ5EA87.
KOiK07968.
TreeFamiTF312834.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR003859. Galactosyl_T.
IPR027791. Galactosyl_T_C.
IPR027995. Galactosyl_T_N.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR19300. PTHR19300. 1 hit.
PfamiPF02709. Glyco_transf_7C. 1 hit.
PF13733. Glyco_transf_7N. 1 hit.
[Graphical view]
PRINTSiPR02050. B14GALTRFASE.
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5EA87-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRRLLERPC TLALLVGSQL AVMMYLSLGG FRSLSALFGR EQEPAFDYSH
60 70 80 90 100
PHDVYSNLSH MPGAPVAPGG LPAPQGLPYC PKRSPLLVGP ISVSFSPVPS
110 120 130 140 150
LAEIVERNPR VEPGGRYRPA RCEPRSRTAI IVPHRAREHH LRLLLYHLHP
160 170 180 190 200
FLQRQQLAYG IYVIHQAGNG TFNRAKLLNV GVREALRDEE WDCLFLHDVD
210 220 230 240 250
LLPENDHNLY VCDPRGPRHV AVAMNKFGYS LPYPQYFGGV SALTPDQYLK
260 270 280 290 300
MNGFPNEYWG WGGEDDDIAT RVRLAGMKIS RPPTSVGHYK MVKHRGDKGN
310 320 330 340 350
EENPHRFDLL VRTQNSWTQD GMNSLTYQLL SRELGPLYTN ITADIGTDPR
360 370 380 390
GPRTSSGPHY PPGSSQAFRQ EMLQRRPPAR PGPLPTANHT APHGSH
Length:396
Mass (Da):44,371
Last modified:June 21, 2005 - v2
Checksum:i8C2611ED7C26250D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti82 – 821K → E in AAX08699 (PubMed:16305752).Curated
Sequence conflicti82 – 821K → E in AAI23719 (Ref. 2) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT020682 mRNA. Translation: AAX08699.1.
BT020868 mRNA. Translation: AAX08885.1.
BT020916 mRNA. Translation: AAX08933.1.
BT026158 mRNA. Translation: ABG66997.1.
BC123718 mRNA. Translation: AAI23719.1.
RefSeqiNP_001015609.1. NM_001015609.1.
XP_005203558.1. XM_005203501.3.
XP_005203559.1. XM_005203502.3.
XP_010801059.1. XM_010802757.2.
UniGeneiBt.4375.

Genome annotation databases

GeneIDi515771.
KEGGibta:515771.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT020682 mRNA. Translation: AAX08699.1.
BT020868 mRNA. Translation: AAX08885.1.
BT020916 mRNA. Translation: AAX08933.1.
BT026158 mRNA. Translation: ABG66997.1.
BC123718 mRNA. Translation: AAI23719.1.
RefSeqiNP_001015609.1. NM_001015609.1.
XP_005203558.1. XM_005203501.3.
XP_005203559.1. XM_005203502.3.
XP_010801059.1. XM_010802757.2.
UniGeneiBt.4375.

3D structure databases

ProteinModelPortaliQ5EA87.
SMRiQ5EA87. Positions 77-347.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000049355.

Protein family/group databases

CAZyiGT7. Glycosyltransferase Family 7.

Proteomic databases

PaxDbiQ5EA87.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi515771.
KEGGibta:515771.

Organism-specific databases

CTDi8703.

Phylogenomic databases

eggNOGiKOG3916. Eukaryota.
ENOG410ZYYA. LUCA.
HOGENOMiHOG000231027.
HOVERGENiHBG058334.
InParanoidiQ5EA87.
KOiK07968.
TreeFamiTF312834.

Enzyme and pathway databases

UniPathwayiUPA00378.

Gene expression databases

BgeeiENSBTAG00000021897.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR003859. Galactosyl_T.
IPR027791. Galactosyl_T_C.
IPR027995. Galactosyl_T_N.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR19300. PTHR19300. 1 hit.
PfamiPF02709. Glyco_transf_7C. 1 hit.
PF13733. Glyco_transf_7N. 1 hit.
[Graphical view]
PRINTSiPR02050. B14GALTRFASE.
SUPFAMiSSF53448. SSF53448. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiB4GT3_BOVIN
AccessioniPrimary (citable) accession number: Q5EA87
Secondary accession number(s): Q08DJ2, Q0V8R0, Q5E9K4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: June 21, 2005
Last modified: September 7, 2016
This is version 96 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.