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Protein

UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase

Gene

DPAGT1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the initial step in the synthesis of dolichol-P-P-oligosaccharides.By similarity

Catalytic activityi

UDP-N-acetyl-D-glucosamine + dolichyl phosphate = UMP + N-acetyl-D-glucosaminyl-diphosphodolichol.

Pathwayi

GO - Molecular functioni

  1. phospho-N-acetylmuramoyl-pentapeptide-transferase activity Source: InterPro
  2. transferase activity, transferring glycosyl groups Source: UniProtKB-KW
  3. UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase activity Source: UniProtKB

GO - Biological processi

  1. dolichol biosynthetic process Source: UniProtKB
  2. dolichol metabolic process Source: GO_Central
  3. polysaccharide biosynthetic process Source: GO_Central
  4. protein N-linked glycosylation Source: GO_Central
  5. protein oligomerization Source: UniProtKB
  6. UDP-N-acetylglucosamine metabolic process Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

ReactomeiREACT_322372. Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein.
UniPathwayiUPA00378.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase (EC:2.7.8.15)
Alternative name(s):
GlcNAc-1-P transferase
Short name:
G1PT
Short name:
GPT
N-acetylglucosamine-1-phosphate transferase
Gene namesi
Name:DPAGT1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136 Componenti: Chromosome 15

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 66LumenalSequence Analysis
Transmembranei7 – 3226HelicalSequence AnalysisAdd
BLAST
Topological domaini33 – 5725CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei58 – 7922HelicalSequence AnalysisAdd
BLAST
Topological domaini80 – 9415LumenalSequence AnalysisAdd
BLAST
Transmembranei95 – 11420HelicalSequence AnalysisAdd
BLAST
Topological domaini115 – 12511CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei126 – 14520HelicalSequence AnalysisAdd
BLAST
Topological domaini146 – 16419LumenalSequence AnalysisAdd
BLAST
Transmembranei165 – 18420HelicalSequence AnalysisAdd
BLAST
Topological domaini185 – 19410CytoplasmicSequence Analysis
Transmembranei195 – 21117HelicalSequence AnalysisAdd
BLAST
Topological domaini212 – 22110LumenalSequence Analysis
Transmembranei222 – 24019HelicalSequence AnalysisAdd
BLAST
Topological domaini241 – 25212CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei253 – 26917HelicalSequence AnalysisAdd
BLAST
Topological domaini270 – 2745LumenalSequence Analysis
Transmembranei275 – 29420HelicalSequence AnalysisAdd
BLAST
Topological domaini295 – 37884CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei379 – 39719HelicalSequence AnalysisAdd
BLAST
Topological domaini398 – 40811LumenalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: UniProtKB
  2. integral component of membrane Source: UniProtKB
  3. membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 408408UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferasePRO_0000314781Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi146 – 1461N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiQ5EA65.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi67 – 7913Dolichol recognitionAdd
BLAST
Motifi222 – 23413Dolichol recognitionAdd
BLAST

Sequence similaritiesi

Belongs to the glycosyltransferase 4 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0472.
GeneTreeiENSGT00390000011424.
HOGENOMiHOG000163915.
HOVERGENiHBG000846.
InParanoidiQ5EA65.
KOiK01001.
OMAiIHERNLT.
OrthoDBiEOG73804W.
TreeFamiTF313734.

Family and domain databases

InterProiIPR000715. Glycosyl_transferase_4.
[Graphical view]
PfamiPF00953. Glycos_transf_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5EA65-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWAFPELPMP LLVNLIGSLM GFVATVTLIP AFRGHFIAAR LCGQDLNKSS
60 70 80 90 100
REQIPESQGV ISGAVFLIIL FCFIPFPFLN CFVEQQCKAF PHHEFVALIG
110 120 130 140 150
ALLAICCMIF LGFADDVLNL RWRHKLLLPT AASLPLLMVY FTNFGNTTIV
160 170 180 190 200
VPKPLRPILG LHLDLGILYY VYMGLLAVFC TNAINILAGI NGLEAGQSLV
210 220 230 240 250
ISASIIVFNL VELDGDYRDD HIFSLYFMIP FFFTTLGLLY HNWYPSRVFV
260 270 280 290 300
GDTFCYFAGM TFAVVGILGH FSKTMLLFFM PQVFNFLYSL PQLLHIIPCP
310 320 330 340 350
RHRMPRLNTK TGKLEMSYSK FKTKSLSFLG TFILKVAENL GLLTVRHSED
360 370 380 390 400
EDGAFTECNN MTLINLLLKV FGPMHERNLT LLLLLLQVVG SAVTFSIRYQ

LVRLFYDV
Length:408
Mass (Da):46,045
Last modified:March 15, 2005 - v1
Checksum:i9BD875493287FB1C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti312 – 3121G → D in AAI02418 (Ref. 2) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT020704 mRNA. Translation: AAX08721.1.
BC102417 mRNA. Translation: AAI02418.1.
RefSeqiNP_001015664.1. NM_001015664.1.
UniGeneiBt.5711.

Genome annotation databases

EnsembliENSBTAT00000007065; ENSBTAP00000007065; ENSBTAG00000005371.
GeneIDi537812.
KEGGibta:537812.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT020704 mRNA. Translation: AAX08721.1.
BC102417 mRNA. Translation: AAI02418.1.
RefSeqiNP_001015664.1. NM_001015664.1.
UniGeneiBt.5711.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ5EA65.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000007065; ENSBTAP00000007065; ENSBTAG00000005371.
GeneIDi537812.
KEGGibta:537812.

Organism-specific databases

CTDi1798.

Phylogenomic databases

eggNOGiCOG0472.
GeneTreeiENSGT00390000011424.
HOGENOMiHOG000163915.
HOVERGENiHBG000846.
InParanoidiQ5EA65.
KOiK01001.
OMAiIHERNLT.
OrthoDBiEOG73804W.
TreeFamiTF313734.

Enzyme and pathway databases

UniPathwayiUPA00378.
ReactomeiREACT_322372. Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein.

Miscellaneous databases

NextBioi20877222.

Family and domain databases

InterProiIPR000715. Glycosyl_transferase_4.
[Graphical view]
PfamiPF00953. Glycos_transf_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Crossbred X Angus.
    Tissue: Ileum.

Entry informationi

Entry nameiGPT_BOVIN
AccessioniPrimary (citable) accession number: Q5EA65
Secondary accession number(s): Q3T0F3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: March 15, 2005
Last modified: April 1, 2015
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.