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Q5EA65

- GPT_BOVIN

UniProt

Q5EA65 - GPT_BOVIN

Protein

UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase

Gene

DPAGT1

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 62 (01 Oct 2014)
      Sequence version 1 (15 Mar 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the initial step in the synthesis of dolichol-P-P-oligosaccharides.By similarity

    Catalytic activityi

    UDP-N-acetyl-D-glucosamine + dolichyl phosphate = UMP + N-acetyl-D-glucosaminyl-diphosphodolichol.

    Pathwayi

    GO - Molecular functioni

    1. phospho-N-acetylmuramoyl-pentapeptide-transferase activity Source: InterPro
    2. transferase activity, transferring glycosyl groups Source: UniProtKB-KW
    3. UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase activity Source: UniProtKB

    GO - Biological processi

    1. dolichol biosynthetic process Source: UniProtKB
    2. protein N-linked glycosylation Source: Ensembl
    3. protein oligomerization Source: UniProtKB

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Enzyme and pathway databases

    ReactomeiREACT_220681. Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein.
    UniPathwayiUPA00378.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase (EC:2.7.8.15)
    Alternative name(s):
    GlcNAc-1-P transferase
    Short name:
    G1PT
    Short name:
    GPT
    N-acetylglucosamine-1-phosphate transferase
    Gene namesi
    Name:DPAGT1
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 15

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB
    2. integral component of membrane Source: UniProtKB
    3. membrane Source: UniProtKB

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 408408UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferasePRO_0000314781Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi146 – 1461N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PRIDEiQ5EA65.

    Structurei

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 66LumenalSequence Analysis
    Topological domaini33 – 5725CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini80 – 9415LumenalSequence AnalysisAdd
    BLAST
    Topological domaini115 – 12511CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini146 – 16419LumenalSequence AnalysisAdd
    BLAST
    Topological domaini185 – 19410CytoplasmicSequence Analysis
    Topological domaini212 – 22110LumenalSequence Analysis
    Topological domaini241 – 25212CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini270 – 2745LumenalSequence Analysis
    Topological domaini295 – 37884CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini398 – 40811LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei7 – 3226HelicalSequence AnalysisAdd
    BLAST
    Transmembranei58 – 7922HelicalSequence AnalysisAdd
    BLAST
    Transmembranei95 – 11420HelicalSequence AnalysisAdd
    BLAST
    Transmembranei126 – 14520HelicalSequence AnalysisAdd
    BLAST
    Transmembranei165 – 18420HelicalSequence AnalysisAdd
    BLAST
    Transmembranei195 – 21117HelicalSequence AnalysisAdd
    BLAST
    Transmembranei222 – 24019HelicalSequence AnalysisAdd
    BLAST
    Transmembranei253 – 26917HelicalSequence AnalysisAdd
    BLAST
    Transmembranei275 – 29420HelicalSequence AnalysisAdd
    BLAST
    Transmembranei379 – 39719HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi67 – 7913Dolichol recognitionAdd
    BLAST
    Motifi222 – 23413Dolichol recognitionAdd
    BLAST

    Sequence similaritiesi

    Belongs to the glycosyltransferase 4 family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0472.
    GeneTreeiENSGT00390000011424.
    HOGENOMiHOG000163915.
    HOVERGENiHBG000846.
    InParanoidiQ5EA65.
    KOiK01001.
    OMAiIHERNLT.
    OrthoDBiEOG73804W.
    TreeFamiTF313734.

    Family and domain databases

    InterProiIPR000715. Glycosyl_transferase_4.
    [Graphical view]
    PfamiPF00953. Glycos_transf_4. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q5EA65-1 [UniParc]FASTAAdd to Basket

    « Hide

    MWAFPELPMP LLVNLIGSLM GFVATVTLIP AFRGHFIAAR LCGQDLNKSS    50
    REQIPESQGV ISGAVFLIIL FCFIPFPFLN CFVEQQCKAF PHHEFVALIG 100
    ALLAICCMIF LGFADDVLNL RWRHKLLLPT AASLPLLMVY FTNFGNTTIV 150
    VPKPLRPILG LHLDLGILYY VYMGLLAVFC TNAINILAGI NGLEAGQSLV 200
    ISASIIVFNL VELDGDYRDD HIFSLYFMIP FFFTTLGLLY HNWYPSRVFV 250
    GDTFCYFAGM TFAVVGILGH FSKTMLLFFM PQVFNFLYSL PQLLHIIPCP 300
    RHRMPRLNTK TGKLEMSYSK FKTKSLSFLG TFILKVAENL GLLTVRHSED 350
    EDGAFTECNN MTLINLLLKV FGPMHERNLT LLLLLLQVVG SAVTFSIRYQ 400
    LVRLFYDV 408
    Length:408
    Mass (Da):46,045
    Last modified:March 15, 2005 - v1
    Checksum:i9BD875493287FB1C
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti312 – 3121G → D in AAI02418. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BT020704 mRNA. Translation: AAX08721.1.
    BC102417 mRNA. Translation: AAI02418.1.
    RefSeqiNP_001015664.1. NM_001015664.1.
    UniGeneiBt.5711.

    Genome annotation databases

    EnsembliENSBTAT00000007065; ENSBTAP00000007065; ENSBTAG00000005371.
    GeneIDi537812.
    KEGGibta:537812.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BT020704 mRNA. Translation: AAX08721.1 .
    BC102417 mRNA. Translation: AAI02418.1 .
    RefSeqi NP_001015664.1. NM_001015664.1.
    UniGenei Bt.5711.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi Q5EA65.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000007065 ; ENSBTAP00000007065 ; ENSBTAG00000005371 .
    GeneIDi 537812.
    KEGGi bta:537812.

    Organism-specific databases

    CTDi 1798.

    Phylogenomic databases

    eggNOGi COG0472.
    GeneTreei ENSGT00390000011424.
    HOGENOMi HOG000163915.
    HOVERGENi HBG000846.
    InParanoidi Q5EA65.
    KOi K01001.
    OMAi IHERNLT.
    OrthoDBi EOG73804W.
    TreeFami TF313734.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .
    Reactomei REACT_220681. Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein.

    Miscellaneous databases

    NextBioi 20877222.

    Family and domain databases

    InterProi IPR000715. Glycosyl_transferase_4.
    [Graphical view ]
    Pfami PF00953. Glycos_transf_4. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    2. NIH - Mammalian Gene Collection (MGC) project
      Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Crossbred X Angus.
      Tissue: Ileum.

    Entry informationi

    Entry nameiGPT_BOVIN
    AccessioniPrimary (citable) accession number: Q5EA65
    Secondary accession number(s): Q3T0F3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: March 15, 2005
    Last modified: October 1, 2014
    This is version 62 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3