Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase

Gene

DPAGT1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the initial step in the synthesis of dolichol-P-P-oligosaccharides.By similarity

Catalytic activityi

UDP-N-acetyl-D-glucosamine + dolichyl phosphate = UMP + N-acetyl-D-glucosaminyl-diphosphodolichol.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

ReactomeiR-BTA-446193. Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein.
UniPathwayiUPA00378.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase (EC:2.7.8.15)
Alternative name(s):
GlcNAc-1-P transferase
Short name:
G1PT
Short name:
GPT
N-acetylglucosamine-1-phosphate transferase
Gene namesi
Name:DPAGT1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 15

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 6LumenalSequence analysis6
Transmembranei7 – 32HelicalSequence analysisAdd BLAST26
Topological domaini33 – 57CytoplasmicSequence analysisAdd BLAST25
Transmembranei58 – 79HelicalSequence analysisAdd BLAST22
Topological domaini80 – 94LumenalSequence analysisAdd BLAST15
Transmembranei95 – 114HelicalSequence analysisAdd BLAST20
Topological domaini115 – 125CytoplasmicSequence analysisAdd BLAST11
Transmembranei126 – 145HelicalSequence analysisAdd BLAST20
Topological domaini146 – 164LumenalSequence analysisAdd BLAST19
Transmembranei165 – 184HelicalSequence analysisAdd BLAST20
Topological domaini185 – 194CytoplasmicSequence analysis10
Transmembranei195 – 211HelicalSequence analysisAdd BLAST17
Topological domaini212 – 221LumenalSequence analysis10
Transmembranei222 – 240HelicalSequence analysisAdd BLAST19
Topological domaini241 – 252CytoplasmicSequence analysisAdd BLAST12
Transmembranei253 – 269HelicalSequence analysisAdd BLAST17
Topological domaini270 – 274LumenalSequence analysis5
Transmembranei275 – 294HelicalSequence analysisAdd BLAST20
Topological domaini295 – 378CytoplasmicSequence analysisAdd BLAST84
Transmembranei379 – 397HelicalSequence analysisAdd BLAST19
Topological domaini398 – 408LumenalSequence analysisAdd BLAST11

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003147811 – 408UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferaseAdd BLAST408

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi146N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ5EA65.
PRIDEiQ5EA65.

Expressioni

Gene expression databases

BgeeiENSBTAG00000005371.

Interactioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000007065.

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi67 – 79Dolichol recognitionAdd BLAST13
Motifi222 – 234Dolichol recognitionAdd BLAST13

Sequence similaritiesi

Belongs to the glycosyltransferase 4 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2788. Eukaryota.
COG0472. LUCA.
GeneTreeiENSGT00390000011424.
HOGENOMiHOG000163915.
HOVERGENiHBG000846.
InParanoidiQ5EA65.
KOiK01001.
OMAiWAFPELP.
OrthoDBiEOG091G0A0M.
TreeFamiTF313734.

Family and domain databases

CDDicd06855. GT_GPT_euk. 1 hit.
InterProiIPR000715. Glycosyl_transferase_4.
IPR033895. GPT.
[Graphical view]
PANTHERiPTHR10571. PTHR10571. 1 hit.
PfamiPF00953. Glycos_transf_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5EA65-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWAFPELPMP LLVNLIGSLM GFVATVTLIP AFRGHFIAAR LCGQDLNKSS
60 70 80 90 100
REQIPESQGV ISGAVFLIIL FCFIPFPFLN CFVEQQCKAF PHHEFVALIG
110 120 130 140 150
ALLAICCMIF LGFADDVLNL RWRHKLLLPT AASLPLLMVY FTNFGNTTIV
160 170 180 190 200
VPKPLRPILG LHLDLGILYY VYMGLLAVFC TNAINILAGI NGLEAGQSLV
210 220 230 240 250
ISASIIVFNL VELDGDYRDD HIFSLYFMIP FFFTTLGLLY HNWYPSRVFV
260 270 280 290 300
GDTFCYFAGM TFAVVGILGH FSKTMLLFFM PQVFNFLYSL PQLLHIIPCP
310 320 330 340 350
RHRMPRLNTK TGKLEMSYSK FKTKSLSFLG TFILKVAENL GLLTVRHSED
360 370 380 390 400
EDGAFTECNN MTLINLLLKV FGPMHERNLT LLLLLLQVVG SAVTFSIRYQ

LVRLFYDV
Length:408
Mass (Da):46,045
Last modified:March 15, 2005 - v1
Checksum:i9BD875493287FB1C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti312G → D in AAI02418 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT020704 mRNA. Translation: AAX08721.1.
BC102417 mRNA. Translation: AAI02418.1.
RefSeqiNP_001015664.1. NM_001015664.1.
UniGeneiBt.5711.

Genome annotation databases

EnsembliENSBTAT00000007065; ENSBTAP00000007065; ENSBTAG00000005371.
GeneIDi537812.
KEGGibta:537812.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT020704 mRNA. Translation: AAX08721.1.
BC102417 mRNA. Translation: AAI02418.1.
RefSeqiNP_001015664.1. NM_001015664.1.
UniGeneiBt.5711.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000007065.

Proteomic databases

PaxDbiQ5EA65.
PRIDEiQ5EA65.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000007065; ENSBTAP00000007065; ENSBTAG00000005371.
GeneIDi537812.
KEGGibta:537812.

Organism-specific databases

CTDi1798.

Phylogenomic databases

eggNOGiKOG2788. Eukaryota.
COG0472. LUCA.
GeneTreeiENSGT00390000011424.
HOGENOMiHOG000163915.
HOVERGENiHBG000846.
InParanoidiQ5EA65.
KOiK01001.
OMAiWAFPELP.
OrthoDBiEOG091G0A0M.
TreeFamiTF313734.

Enzyme and pathway databases

UniPathwayiUPA00378.
ReactomeiR-BTA-446193. Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein.

Gene expression databases

BgeeiENSBTAG00000005371.

Family and domain databases

CDDicd06855. GT_GPT_euk. 1 hit.
InterProiIPR000715. Glycosyl_transferase_4.
IPR033895. GPT.
[Graphical view]
PANTHERiPTHR10571. PTHR10571. 1 hit.
PfamiPF00953. Glycos_transf_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGPT_BOVIN
AccessioniPrimary (citable) accession number: Q5EA65
Secondary accession number(s): Q3T0F3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: March 15, 2005
Last modified: November 30, 2016
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.