Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q5EA41 (GALT6_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Polypeptide N-acetylgalactosaminyltransferase 6

EC=2.4.1.41
Alternative name(s):
Polypeptide GalNAc transferase 6
Short name=GalNAc-T6
Short name=pp-GaNTase 6
Protein-UDP acetylgalactosaminyltransferase 6
UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 6
Gene names
Name:GALNT6
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length622 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. May participate in synthesis of oncofetal fibronectin. Has activity toward Muc1a, Muc2, EA2 and fibronectin peptides By similarity.

Catalytic activity

UDP-N-acetyl-alpha-D-galactosamine + polypeptide = UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide.

Cofactor

Manganese By similarity.

Pathway

Protein modification; protein glycosylation.

Subcellular location

Golgi apparatus membrane; Single-pass type II membrane protein By similarity.

Domain

There are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding By similarity.

The ricin B-type lectin domain binds to GalNAc and contributes to the glycopeptide specificity By similarity.

Sequence similarities

Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily.

Contains 1 ricin B-type lectin domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 622622Polypeptide N-acetylgalactosaminyltransferase 6
PRO_0000059113

Regions

Topological domain1 – 88Cytoplasmic Potential
Transmembrane9 – 2820Helical; Signal-anchor for type II membrane protein; Potential
Topological domain29 – 622594Lumenal Potential
Domain496 – 622127Ricin B-type lectin
Region176 – 285110Catalytic subdomain A
Region348 – 41063Catalytic subdomain B

Sites

Metal binding2691Manganese By similarity
Metal binding2711Manganese By similarity
Metal binding4071Manganese By similarity
Binding site2171Substrate By similarity
Binding site2461Substrate By similarity
Binding site3791Substrate By similarity
Binding site4101Substrate By similarity

Amino acid modifications

Glycosylation861N-linked (GlcNAc...) Potential
Glycosylation4761N-linked (GlcNAc...) Potential
Disulfide bond165 ↔ 402 By similarity
Disulfide bond393 ↔ 474 By similarity
Disulfide bond509 ↔ 527 By similarity
Disulfide bond553 ↔ 566 By similarity
Disulfide bond597 ↔ 610 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5EA41 [UniParc].

Last modified March 15, 2005. Version 1.
Checksum: 05010D38D433A376

FASTA62271,138
        10         20         30         40         50         60 
MRLLRRRHMA VRLVMVGSAF VLFLFILQRD VSGREQATEK PWLRSLVSQK DHVLDLMLGA 

        70         80         90        100        110        120 
VHNLRDSMPK FQIRAPEPQQ TLASTNQSCL PGFYTPAELK PFWERPPQDP NGPGADGKAF 

       130        140        150        160        170        180 
QKKEWTPQET QEKEEGYKKH CFNAFASDRI SLQRALGPDT RPPECVDQKF RRCPPLPATS 

       190        200        210        220        230        240 
VIIVFHNEAW STLLRTVYSV LHTTPAILLK EIILVDDAST DEYLKEPLER YVKQLQVVQV 

       250        260        270        280        290        300 
VRQQERKGLI TARLLGASVA QAEVLTFLDA HCECFHGWLE PLLARIAEDE TVVVSPNIVT 

       310        320        330        340        350        360 
IDLNTFEFSK PVQRGRVQSR GNFDWSLTFG WEVLPAREKQ RRKDETYPIK SPTFAGGLFS 

       370        380        390        400        410        420 
ISKSYFEHIG TYDNQMEIWG GENVEMSFRV WQCGGQLEII PCSVVGHVFR TKSPHTFPKG 

       430        440        450        460        470        480 
INVIARNQVR LAEVWMDGYK EIFYRRNLQA AQMAREKSFG DISERLQLRE RLNCHNFSWF 

       490        500        510        520        530        540 
LDNVYPEMFV PDLKPTFFGA LKNLGVDHCL DVGENNNGGK PLILYTCHGL GGNQYFEYTT 

       550        560        570        580        590        600 
QRDLRHNIAK QLCLHASAGT LGLRSCHFTG KNSQVPKDEE WEFTQDQLIR NSGSGTCLTS 

       610        620 
KDKKPVMATC NPSDPHQHWL FI 

« Hide

References

[1]"Characterization of 954 bovine full-CDS cDNA sequences."
Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L.
BMC Genomics 6:166-166(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BT020728 mRNA. Translation: AAX08745.1.
RefSeqNP_001015534.1. NM_001015534.1.
UniGeneBt.1639.

3D structure databases

ProteinModelPortalQ5EA41.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyCBM13. Carbohydrate-Binding Module Family 13.
GT27. Glycosyltransferase Family 27.

Proteomic databases

PRIDEQ5EA41.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID506903.
KEGGbta:506903.

Organism-specific databases

CTD11226.

Phylogenomic databases

eggNOGNOG239675.
HOGENOMHOG000038227.
HOVERGENHBG051699.
InParanoidQ5EA41.
KOK00710.

Enzyme and pathway databases

UniPathwayUPA00378.

Family and domain databases

Gene3D3.90.550.10. 1 hit.
InterProIPR001173. Glyco_trans_2-like.
IPR029044. Nucleotide-diphossugar_trans.
IPR000772. Ricin_B_lectin.
[Graphical view]
PfamPF00535. Glycos_transf_2. 1 hit.
PF00652. Ricin_B_lectin. 1 hit.
[Graphical view]
SMARTSM00458. RICIN. 1 hit.
[Graphical view]
SUPFAMSSF50370. SSF50370. 1 hit.
SSF53448. SSF53448. 1 hit.
PROSITEPS50231. RICIN_B_LECTIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20867793.

Entry information

Entry nameGALT6_BOVIN
AccessionPrimary (citable) accession number: Q5EA41
Entry history
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: March 15, 2005
Last modified: June 11, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways