Reviewed, UniProtKB/Swiss-Prot Q5EA20 (HPPD_BOVIN)

Names and origin

Protein names

Recommended name:
    4-hydroxyphenylpyruvate dioxygenase
    EC=1.13.11.27
Alternative name(s):
    4-hydroxyphenylpyruvic acid oxidase
      Short name=HPPDase
      Short name=4HPPD
      Short name=HPD

Gene names

Name:

HPD

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	393 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	4-hydroxyphenylpyruvate + O2 = homogentisate + CO2.
Cofactor	Binds 1 iron ion per subunit By similarity.
Pathway	Amino-acid degradation; L-phenylalanine degradation; acetoacetate and fumarate from L-phenylalanine: step 3/6.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the 4HPPD family.

Ontologies

Keywords
Biological process	Phenylalanine catabolism Tyrosine catabolism
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
PTM	Acetylation Phosphoprotein
Gene Ontology (GO)
Biological process	L-phenylalanine catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tyrosine catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4-hydroxyphenylpyruvate dioxygenase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 393	392	4-hydroxyphenylpyruvate dioxygenase		PRO_0000088387
Sites
	Metal binding	183	1	Iron By similarity
	Metal binding	266	1	Iron By similarity
	Metal binding	349	1	Iron By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylthreonine By similarity
	Modified residue	250	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5EA20-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 03036A1CD0B7BBD1	FASTA	393	44,963
	10 20 30 40 50 60 MTTYSDKGEK PERGRFLHFH SVTFWVGNAK QAASYYCSKL GFEPLAYKGL ETGSREVVSH 70 80 90 100 110 120 VVKQGQIVFV FSSALNPWNK EMGDHLVKHG DGVKDIAFEV EDCDYIVQKA RERGAKIVRE 130 140 150 160 170 180 PWVEQDKLGK VKFAVLQTYG DTTHTLVEKM NYTGRFLPGF EAPPFMDPQL SKLPSCSLEI 190 200 210 220 230 240 IDHIVGNQPD QEMVSASEWY LKNLQFHRFW SVDDTQVHTE YSSLRSVVVA NYEESIKMPI 250 260 270 280 290 300 NEPAPGKKKS QIQEYVDYNG GAGVQHIALK TKDIITAIRH LRERGVEFLA VPSTYYKQLR 310 320 330 340 350 360 EKLKMAKIRV KENIDILEEL KILVDYDEKG YLLQIFTKPM QDRPTLFLEV IQRHNHQGFG 370 380 390 AGNFNSLFKA FEEEQDLRGN LTDMEPNGVV SGM

References

[1]	"Characterization of 954 bovine full-CDS cDNA sequences." Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L. BMC Genomics 6:166-166(2005) [PubMed: 16305752] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[2]	NIH - Mammalian Gene Collection (MGC) project Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Rumen reticulum.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BT020749 mRNA. Translation: AAX08766.1. BC105225 mRNA. Translation: AAI05226.1.
IPI	IPI00700288.
RefSeq	NP_001015611.1.
UniGene	Bt.19922
3D structure databases
SMR	Q5EA20. Positions 7-365, 8-366.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5EA20.
Genome annotation databases
Ensembl	ENSBTAT00000005469; ENSBTAP00000005469; ENSBTAG00000004175; Bos taurus. [Genome view]
GeneID	516058.
KEGG	bta:516058.
Organism-specific databases
CTD	516058.
Phylogenomic databases
HOVERGEN	Q5EA20.
OMA	VQDRPTV.
Enzyme and pathway databases
BRENDA	1.13.11.27. 251.
Family and domain databases
InterPro	IPR005956. 4OHPhenylPyrv_dOase. IPR004360. Glyas_bleo-R_dOase. [Graphical view]
PANTHER	PTHR11959. HPP_dOase. 1 hit.
Pfam	PF00903. Glyoxalase. 2 hits. [Graphical view]
PIRSF	PIRSF009283. HPP_dOase. 1 hit.
TIGRFAMs	TIGR01263. 4HPPD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HPPD_BOVIN

Accession

Primary (citable) accession number: Q5EA20

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	January 23, 2007
Last modified:	November 3, 2009
This is version 43 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)