Q5EA20 (HPPD_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-hydroxyphenylpyruvate dioxygenase
EC=1.13.11.27

Alternative name(s):
4-hydroxyphenylpyruvic acid oxidase
Short name=4HPPD
Short name=HPD
Short name=HPPDase

Gene names

Name:

HPD

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	393 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Key enzyme in the degradation of tyrosine By similarity.
Catalytic activity	4-hydroxyphenylpyruvate + O2 = homogentisate + CO2.
Cofactor	Binds 1 iron ion per subunit By similarity.
Pathway	Amino-acid degradation; L-phenylalanine degradation; acetoacetate and fumarate from L-phenylalanine: step 3/6.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the 4HPPD family.

Ontologies

Keywords
Biological process	Phenylalanine catabolism Tyrosine catabolism
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	L-phenylalanine catabolic process Inferred from electronic annotation. Source: UniProtKB-KW tyrosine catabolic process Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	4-hydroxyphenylpyruvate dioxygenase activity Inferred from sequence or structural similarity. Source: UniProtKB metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 393	392	4-hydroxyphenylpyruvate dioxygenase		PRO_0000088387
Sites
	Metal binding	183	1	Iron By similarity
	Metal binding	266	1	Iron By similarity
	Metal binding	349	1	Iron By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylthreonine By similarity
	Modified residue	250	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5EA20 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 03036A1CD0B7BBD1	FASTA	393	44,963
	10 20 30 40 50 60 MTTYSDKGEK PERGRFLHFH SVTFWVGNAK QAASYYCSKL GFEPLAYKGL ETGSREVVSH 70 80 90 100 110 120 VVKQGQIVFV FSSALNPWNK EMGDHLVKHG DGVKDIAFEV EDCDYIVQKA RERGAKIVRE 130 140 150 160 170 180 PWVEQDKLGK VKFAVLQTYG DTTHTLVEKM NYTGRFLPGF EAPPFMDPQL SKLPSCSLEI 190 200 210 220 230 240 IDHIVGNQPD QEMVSASEWY LKNLQFHRFW SVDDTQVHTE YSSLRSVVVA NYEESIKMPI 250 260 270 280 290 300 NEPAPGKKKS QIQEYVDYNG GAGVQHIALK TKDIITAIRH LRERGVEFLA VPSTYYKQLR 310 320 330 340 350 360 EKLKMAKIRV KENIDILEEL KILVDYDEKG YLLQIFTKPM QDRPTLFLEV IQRHNHQGFG 370 380 390 AGNFNSLFKA FEEEQDLRGN LTDMEPNGVV SGM

References

[1]	"Characterization of 954 bovine full-CDS cDNA sequences." Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L. BMC Genomics 6:166-166(2005) [PubMed: 16305752] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[2]	NIH - Mammalian Gene Collection (MGC) project Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Rumen reticulum.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BT020749 mRNA. Translation: AAX08766.1. BC105225 mRNA. Translation: AAI05226.1.
IPI	IPI00700288.
RefSeq	NP_001015611.1. NM_001015611.1.
UniGene	Bt.19922.
3D structure databases
ProteinModelPortal	Q5EA20.
SMR	Q5EA20. Positions 8-366.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5EA20.
Proteomic databases
PRIDE	Q5EA20.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000005469; ENSBTAP00000005469; ENSBTAG00000004175.
GeneID	516058.
KEGG	bta:516058.
Organism-specific databases
CTD	3242.
Phylogenomic databases
eggNOG	maNOG11003.
GeneTree	ENSGT00530000063474.
HOVERGEN	HBG005987.
InParanoid	Q5EA20.
OMA	HCVGNVE.
OrthoDB	EOG4Q58PF.
PhylomeDB	Q5EA20.
Family and domain databases
InterPro	IPR005956. 4OHPhenylPyrv_dOase. IPR004360. Glyas_Fos-R_dOase. [Graphical view]
KO	K00457.
PANTHER	PTHR11959. HPP_dOase. 1 hit.
Pfam	PF00903. Glyoxalase. 2 hits. [Graphical view]
PIRSF	PIRSF009283. HPP_dOase. 1 hit.
TIGRFAMs	TIGR01263. 4HPPD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HPPD_BOVIN

Accession

Primary (citable) accession number: Q5EA20

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	January 23, 2007
Last modified:	November 16, 2011
This is version 60 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program