Reviewed, UniProtKB/Swiss-Prot Q5EA01 (B3GN1_BOVIN)

Names and origin

Protein names

Recommended name:
    N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase
    EC=2.4.1.149
Alternative name(s):
    Poly-N-acetyllactosamine extension enzyme
    I-beta-1,3-N-acetylglucosaminyltransferase
      Short name=iGnT
    UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 1

Gene names

Name:	B3GNT1
Synonyms:	B3GNT6

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	415 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Can initiate the synthesis or the elongation of the linear poly-N-acetyllactosaminoglycans By similarity.
Catalytic activity	UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-(1->4)-N-acetyl-D-glucosaminyl-R = UDP + N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-N-acetyl-D-glucosaminyl-R.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein By similarity.
Sequence caution	The sequence AAX46482.1 differs from that shown. Reason: Frameshift at position 387.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Gene Ontology (GO)
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 415	415	N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase		PRO_0000080554
Regions
	Topological domain	1 – 8	8	Cytoplasmic Potential
	Transmembrane	9 – 36	28	Signal-anchor for type II membrane protein Potential
	Topological domain	37 – 415	379	Lumenal Potential
Amino acid modifications
	Glycosylation	204	1	N-linked (GlcNAc...) Potential
	Glycosylation	300	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	178	1	L → P in AAI05207. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q5EA01-1 [UniParc]. Last modified May 30, 2006. Version 2. Checksum: 1B0FB74C8F8266C2	FASTA	415	47,231
	10 20 30 40 50 60 MQMSYAIRCA FYQLLLAALM LVAMLQLLYL SLLSGLHGQE EQDQYFEFFP PSPRSVDQVK 70 80 90 100 110 120 AQLRTALASG GVLDASGDYR VYRGLLKTTM DPNDVILATH ASVDNLLHLS GLLERWEGPL 130 140 150 160 170 180 SVSVFAATKE EAQLATVLTY ALSSHCPDMR ARVAMHLVCP SRYEAAVPDP REPGEFALLR 190 200 210 220 230 240 SCQEVFDKLA RVAQPGVNYA LGTNVSYPNN LLRNLAREGA NYALVIDVDM VPSEGLWRSL 250 260 270 280 290 300 REMLDQSKQW AGTALVVPAF EIRRARRMPM NKNELLQLYQ VGEVRPFYYG LCTPCQAPTN 310 320 330 340 350 360 YSRWVNLPEE TLLRPAYVVP WQDPWEPFYV AGGKVPTFDE RFRQYGFNRI SQACELHVAG 370 380 390 400 410 FDFEVLNEGF LVHKGFKEVL KFHPQKEAEN QHNKILYRQF KQELKAKYPD SPRHC

References

[1]	"Characterization of 954 bovine full-CDS cDNA sequences." Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L. BMC Genomics 6:166-166(2005) [PubMed: 16305752] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[2]	NIH - Mammalian Gene Collection (MGC) project Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Kidney.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BT020768 mRNA. Translation: AAX08785.1. BT021635 mRNA. Translation: AAX46482.1. Frameshift. BC105206 mRNA. Translation: AAI05207.1.
IPI	IPI00702686.
RefSeq	NP_001029980.1.
UniGene	Bt.65469
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q5EA01.
Protein family/group databases
CAZy	GT49. Glycosyltransferase Family 49.
Genome annotation databases
Ensembl	ENSBTAT00000030023; ENSBTAP00000030011; ENSBTAG00000022238; Bos taurus. [Genome view]
GeneID	618055.
KEGG	bta:618055.
Phylogenomic databases
eggNOG	maNOG05861.
HOVERGEN	Q5EA01.
InParanoid	Q5EA01.
OMA	AKYPDSP.
OrthoDB	EOG973S9Z.
PhylomeDB	Q5EA01.
Enzyme and pathway databases
BRENDA	2.4.1.149. 251.
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

B3GN1_BOVIN

Accession

Primary (citable) accession number: Q5EA01
Secondary accession number(s): Q3MHK2, Q58DG2

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	May 30, 2006
Last modified:	January 19, 2010
This is version 40 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)