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Q5EA01 (B3GN1_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase
EC=2.4.1.149
Alternative name(s):
I-beta-1,3-N-acetylglucosaminyltransferase
Short name=iGnT
Poly-N-acetyllactosamine extension enzyme
UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 1
Gene names
Name:B3GNT1
Synonyms:B3GNT6
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length415 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Can initiate the synthesis or the elongation of the linear poly-N-acetyllactosaminoglycans By similarity.

Catalytic activity

UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-(1->4)-N-acetyl-D-glucosaminyl-R = UDP + N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-N-acetyl-D-glucosaminyl-R.

Pathway

Protein modification; protein glycosylation.

Subcellular location

Golgi apparatus membrane; Single-pass type II membrane protein By similarity.

Sequence caution

The sequence AAX46482.1 differs from that shown. Reason: Frameshift at position 387.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 415415N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase
PRO_0000080554

Regions

Topological domain1 – 88Cytoplasmic Potential
Transmembrane9 – 3628Helical; Signal-anchor for type II membrane protein; Potential
Topological domain37 – 415379Lumenal Potential

Amino acid modifications

Glycosylation2041N-linked (GlcNAc...) Potential
Glycosylation3001N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict1781L → P in AAI05207. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q5EA01 [UniParc].

Last modified May 30, 2006. Version 2.
Checksum: 1B0FB74C8F8266C2

FASTA41547,231
        10         20         30         40         50         60 
MQMSYAIRCA FYQLLLAALM LVAMLQLLYL SLLSGLHGQE EQDQYFEFFP PSPRSVDQVK 

        70         80         90        100        110        120 
AQLRTALASG GVLDASGDYR VYRGLLKTTM DPNDVILATH ASVDNLLHLS GLLERWEGPL 

       130        140        150        160        170        180 
SVSVFAATKE EAQLATVLTY ALSSHCPDMR ARVAMHLVCP SRYEAAVPDP REPGEFALLR 

       190        200        210        220        230        240 
SCQEVFDKLA RVAQPGVNYA LGTNVSYPNN LLRNLAREGA NYALVIDVDM VPSEGLWRSL 

       250        260        270        280        290        300 
REMLDQSKQW AGTALVVPAF EIRRARRMPM NKNELLQLYQ VGEVRPFYYG LCTPCQAPTN 

       310        320        330        340        350        360 
YSRWVNLPEE TLLRPAYVVP WQDPWEPFYV AGGKVPTFDE RFRQYGFNRI SQACELHVAG 

       370        380        390        400        410 
FDFEVLNEGF LVHKGFKEVL KFHPQKEAEN QHNKILYRQF KQELKAKYPD SPRHC

« Hide

References

[1]"Characterization of 954 bovine full-CDS cDNA sequences."
Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L.
BMC Genomics 6:166-166(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[2]NIH - Mammalian Gene Collection (MGC) project
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Hereford.
Tissue: Kidney.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BT020768 mRNA. Translation: AAX08785.1.
BT021635 mRNA. Translation: AAX46482.1. Frameshift.
BC105206 mRNA. Translation: AAI05207.1.
IPIIPI00702686.
RefSeqNP_001029980.1. NM_001034808.1.
UniGeneBt.65469.

3D structure databases

ModBaseSearch...

Protein family/group databases

CAZyGT49. Glycosyltransferase Family 49.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSBTAT00000030023; ENSBTAP00000030011; ENSBTAG00000022238.
GeneID618055.
KEGGbta:618055.

Organism-specific databases

CTD11041.

Phylogenomic databases

eggNOGNOG325995.
GeneTreeENSGT00530000063165.
HOGENOMHOG000064521.
HOVERGENHBG080885.
InParanoidQ5EA01.
KOK00741.
OMAAKYPDSP.
OrthoDBEOG42V8GB.

Enzyme and pathway databases

UniPathwayUPA00378.

Family and domain databases

InterProIPR026603. N-AclacN_B-1_3-N-AclacNTrfase.
[Graphical view]
PANTHERPTHR12270:SF3. PTHR12270:SF3. 1 hit.
ProtoNetSearch...

Other

NextBio20900975.

Entry information

Entry nameB3GN1_BOVIN
AccessionPrimary (citable) accession number: Q5EA01
Secondary accession number(s): Q3MHK2, Q58DG2
Entry history
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: May 30, 2006
Last modified: April 3, 2013
This is version 62 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

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