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Q5EA01

- B4GA1_BOVIN

UniProt

Q5EA01 - B4GA1_BOVIN

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Protein

Beta-1,4-glucuronyltransferase 1

Gene

B3GNT1

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Beta-1,4-glucuronyltransferase involved in O-mannosylation of alpha-dystroglycan (DAG1). Transfers a glucuronic acid (GlcA) residue onto a xylose (Xyl) acceptor to produce the glucuronyl-beta-1,4-xylose-beta disaccharide primer, which is further elongated by LARGE, during synthesis of phosphorylated O-mannosyl glycan. Phosphorylated O-mannosyl glycan is a carbohydrate is a carbohydrate structure present in alpha-dystroglycan (DAG1), which is required for binding laminin G-like domain-containing extracellular proteins with high affinity. Required for axon guidance; via its function in O-mannosylation of alpha-dystroglycan (DAG1).By similarity

Cofactori

Mn2+By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi227 – 2271ManganeseBy similarity
Metal bindingi229 – 2291ManganeseBy similarity

GO - Molecular functioni

  1. N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase activity Source: UniProtKB-EC

GO - Biological processi

  1. axon guidance Source: Ensembl
  2. poly-N-acetyllactosamine biosynthetic process Source: Ensembl
  3. protein glycosylation Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_211440. Keratan sulfate biosynthesis.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT49. Glycosyltransferase Family 49.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-1,4-glucuronyltransferase 1By similarity (EC:2.4.1.-By similarity)
Alternative name(s):
I-beta-1,3-N-acetylglucosaminyltransferase
Short name:
iGnT
N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase
Poly-N-acetyllactosamine extension enzyme
UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 1
Gene namesi
Name:B3GNT1
Synonyms:B3GNT6
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome 29

Subcellular locationi

Golgi apparatus membrane By similarity; Single-pass type II membrane protein
Note: Localizes near the trans-Golgi apparatus.By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 88CytoplasmicSequence Analysis
Transmembranei9 – 3628Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini37 – 415379LumenalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. extracellular vesicular exosome Source: Ensembl
  2. Golgi membrane Source: UniProtKB-SubCell
  3. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 415415Beta-1,4-glucuronyltransferase 1PRO_0000080554Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi204 – 2041N-linked (GlcNAc...)Sequence Analysis
Glycosylationi300 – 3001N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Interactioni

Subunit structurei

Interacts with LARGE and GYLTL1B/LARGE2.By similarity

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 49 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG325995.
GeneTreeiENSGT00530000063165.
HOGENOMiHOG000064521.
HOVERGENiHBG080885.
InParanoidiQ5EA01.
KOiK00741.
OMAiMPMSKTE.
OrthoDBiEOG7PVWPF.
TreeFamiTF319168.

Family and domain databases

InterProiIPR026603. N-AclacN_B-1_3-N-AclacNTrfase.
[Graphical view]
PANTHERiPTHR12270:SF20. PTHR12270:SF20. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5EA01-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQMSYAIRCA FYQLLLAALM LVAMLQLLYL SLLSGLHGQE EQDQYFEFFP
60 70 80 90 100
PSPRSVDQVK AQLRTALASG GVLDASGDYR VYRGLLKTTM DPNDVILATH
110 120 130 140 150
ASVDNLLHLS GLLERWEGPL SVSVFAATKE EAQLATVLTY ALSSHCPDMR
160 170 180 190 200
ARVAMHLVCP SRYEAAVPDP REPGEFALLR SCQEVFDKLA RVAQPGVNYA
210 220 230 240 250
LGTNVSYPNN LLRNLAREGA NYALVIDVDM VPSEGLWRSL REMLDQSKQW
260 270 280 290 300
AGTALVVPAF EIRRARRMPM NKNELLQLYQ VGEVRPFYYG LCTPCQAPTN
310 320 330 340 350
YSRWVNLPEE TLLRPAYVVP WQDPWEPFYV AGGKVPTFDE RFRQYGFNRI
360 370 380 390 400
SQACELHVAG FDFEVLNEGF LVHKGFKEVL KFHPQKEAEN QHNKILYRQF
410
KQELKAKYPD SPRHC
Length:415
Mass (Da):47,231
Last modified:May 30, 2006 - v2
Checksum:i1B0FB74C8F8266C2
GO

Sequence cautioni

The sequence AAX46482.1 differs from that shown. Reason: Frameshift at position 387. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti178 – 1781L → P in AAI05207. (PubMed:16305752)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT020768 mRNA. Translation: AAX08785.1.
BT021635 mRNA. Translation: AAX46482.1. Frameshift.
BC105206 mRNA. Translation: AAI05207.1.
RefSeqiNP_001029980.1. NM_001034808.1.
UniGeneiBt.65469.

Genome annotation databases

EnsembliENSBTAT00000030023; ENSBTAP00000030011; ENSBTAG00000022238.
GeneIDi618055.
KEGGibta:618055.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT020768 mRNA. Translation: AAX08785.1 .
BT021635 mRNA. Translation: AAX46482.1 . Frameshift.
BC105206 mRNA. Translation: AAI05207.1 .
RefSeqi NP_001029980.1. NM_001034808.1.
UniGenei Bt.65469.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GT49. Glycosyltransferase Family 49.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSBTAT00000030023 ; ENSBTAP00000030011 ; ENSBTAG00000022238 .
GeneIDi 618055.
KEGGi bta:618055.

Organism-specific databases

CTDi 11041.

Phylogenomic databases

eggNOGi NOG325995.
GeneTreei ENSGT00530000063165.
HOGENOMi HOG000064521.
HOVERGENi HBG080885.
InParanoidi Q5EA01.
KOi K00741.
OMAi MPMSKTE.
OrthoDBi EOG7PVWPF.
TreeFami TF319168.

Enzyme and pathway databases

UniPathwayi UPA00378 .
Reactomei REACT_211440. Keratan sulfate biosynthesis.

Miscellaneous databases

NextBioi 20900975.

Family and domain databases

InterProi IPR026603. N-AclacN_B-1_3-N-AclacNTrfase.
[Graphical view ]
PANTHERi PTHR12270:SF20. PTHR12270:SF20. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Kidney.

Entry informationi

Entry nameiB4GA1_BOVIN
AccessioniPrimary (citable) accession number: Q5EA01
Secondary accession number(s): Q3MHK2, Q58DG2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: May 30, 2006
Last modified: January 7, 2015
This is version 74 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.