Reviewed, UniProtKB/Swiss-Prot Q5E9X2 (MP2K6_BOVIN)

Names and origin

Protein names

Recommended name:
    Dual specificity mitogen-activated protein kinase kinase 6
      Short name=MAP kinase kinase 6
      Short name=MAPKK 6
    EC=2.7.12.2
Alternative name(s):
    MAPK/ERK kinase 6
      Short name=MEK 6

Gene names

Name:

MAP2K6

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	334 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in MAP kinase p38 exclusively By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Enzyme regulation	Probably activated by dual phosphorylation on Ser-207 and Thr-211 By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase Tyrosine-protein kinase
PTM	Acetylation Phosphoprotein
Gene Ontology (GO)
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein tyrosine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 334	334	Dual specificity mitogen-activated protein kinase kinase 6		PRO_0000248967
Regions
	Domain	53 – 314	262	Protein kinase
	Nucleotide binding	59 – 67	9	ATP By similarity
Sites
	Active site	179	1	Proton acceptor By similarity
	Binding site	82	1	ATP By similarity
Amino acid modifications
	Modified residue	27	1	Phosphoserine By similarity
	Modified residue	28	1	Phosphothreonine By similarity
	Modified residue	82	1	N6-acetyllysine By similarity
	Modified residue	207	1	Phosphoserine By similarity
	Modified residue	211	1	Phosphothreonine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5E9X2-1 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: 39C23E5541719C04	FASTA	334	37,578
	10 20 30 40 50 60 MSQSKGKKRN PGLKIPKEAF EQPQTSSTPP RDLDSKACIS IGNQNFEVKA DDLEPIVELG 70 80 90 100 110 120 RGAYGVVEKM RHVPSEQIMA VKRIRATVNS QEQKRLLMDL DISMRTVDCP FTVTFYGALF 130 140 150 160 170 180 REGDVWICME LMDTSLDKFY KQVIDKGQTI PEDILGKIAV SIVKALEHLH SKLSVIHRDV 190 200 210 220 230 240 KPSNVLINAL GQVKMCDFGI SGYLVDSVAK TIDAGCKPYM APERINPELN QKGYSVKSDI 250 260 270 280 290 300 WSLGITMIEL AILRFPYDSW GTPFQQLKQV VEEPSPQLPA DKFSEEFVDF TSQCLKKNSK 310 320 330 ERPTYPELMQ HPFFTLHESK ATDVASFVKS ILGD

References

[1]	"Characterization of 954 bovine full-CDS cDNA sequences." Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L. BMC Genomics 6:166-166(2005) [PubMed: 16305752] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	BT020798 mRNA. Translation: AAX08815.1.
IPI	IPI00690263.
RefSeq	NP_001029217.1.
UniGene	Bt.561
3D structure databases
HSSP	HSSP built from PDB template 1S9I based on UniProtKB P36507.
SMR	Q5E9X2. Positions 9-317.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5E9X2.
Genome annotation databases
Ensembl	ENSBTAT00000002115; ENSBTAP00000002115; ENSBTAG00000001609; Bos taurus. [Genome view]
GeneID	286883.
KEGG	bta:286883.
Organism-specific databases
CTD	286883.
Phylogenomic databases
HOVERGEN	Q5E9X2.
InParanoid	Q5E9X2.
OrthoDB	EOG9Q87GM.
PhylomeDB	Q5E9X2.
Enzyme and pathway databases
BRENDA	2.7.12.2. 251.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_prot_kinase-like_dom. IPR008271. Ser/Thr_prot_kinase_AS. IPR002290. Ser/Thr_prot_kinase_dom. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MP2K6_BOVIN

Accession

Primary (citable) accession number: Q5E9X2

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	March 15, 2005
Last modified:	February 9, 2010
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)