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Q5E9W3

- PYRD_BOVIN

UniProt

Q5E9W3 - PYRD_BOVIN

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Protein

Dihydroorotate dehydrogenase (quinone), mitochondrial

Gene

DHODH

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of dihydroorotate to orotate with quinone as electron acceptor.

Catalytic activityi

(S)-dihydroorotate + a quinone = orotate + a quinol.1 Publication

Cofactori

Binds 1 FMN per subunit.By similarity

Kineticsi

  1. KM=9.2 µM for (S)-dihydroorotate2 Publications
  2. KM=13.6 µM for benzyl-(S)-dihydroorotate2 Publications
  3. KM=19.4 µM for methyl-(S)-dihydroorotate2 Publications
  4. KM=10.8 µM for quinone Q62 Publications
  5. KM=13.2 µM for quinone Q72 Publications

Vmax=72 µmol/min/mg enzyme toward (S)-dihydroorotate2 Publications

Vmax=80 µmol/min/mg enzyme toward benzyl-(S)-dihydroorotate2 Publications

Vmax=63 µmol/min/mg enzyme toward methyl-(S)-dihydroorotate2 Publications

Vmax=82 µmol/min/mg enzyme toward quinone Q62 Publications

Vmax=88 µmol/min/mg enzyme toward quinone Q72 Publications

pH dependencei

Optimum pH is 8.0.2 Publications

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei99 – 991SubstrateBy similarity
Binding sitei119 – 1191FMNBy similarity
Binding sitei180 – 1801FMNBy similarity
Binding sitei211 – 2111FMNBy similarity
Active sitei214 – 2141NucleophileBy similarity
Binding sitei254 – 2541FMNBy similarity
Binding sitei282 – 2821FMN; via carbonyl oxygenBy similarity
Binding sitei305 – 3051FMN; via amide nitrogenBy similarity
Binding sitei334 – 3341FMN; via amide nitrogenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi95 – 995FMNBy similarity
Nucleotide bindingi355 – 3562FMNBy similarity

GO - Molecular functioni

  1. dihydroorotate dehydrogenase activity Source: UniProtKB-EC

GO - Biological processi

  1. 'de novo' pyrimidine nucleobase biosynthetic process Source: InterPro
  2. 'de novo' UMP biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Pyrimidine biosynthesis

Keywords - Ligandi

Flavoprotein, FMN

Enzyme and pathway databases

SABIO-RKQ5E9W3.
UniPathwayiUPA00070; UER00946.

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydroorotate dehydrogenase (quinone), mitochondrial (EC:1.3.5.2)
Short name:
DHOdehase
Alternative name(s):
Dihydroorotate oxidase
Gene namesi
Name:DHODH
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Unplaced

Subcellular locationi

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. mitochondrial inner membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 395395Dihydroorotate dehydrogenase (quinone), mitochondrialPRO_0000233397Add
BLAST
Transit peptidei1 – 1010Mitochondrion; not cleavedBy similarity

Post-translational modificationi

The uncleaved transit peptide is required for mitochondrial targeting and proper membrane integration.By similarity

Proteomic databases

PRIDEiQ5E9W3.

Interactioni

Subunit structurei

Monomer.

Structurei

3D structure databases

ProteinModelPortaliQ5E9W3.
SMRiQ5E9W3. Positions 30-395.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1010Mitochondrial matrixBy similarity
Topological domaini31 – 395365Mitochondrial intermembraneBy similarityAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei11 – 3020HelicalBy similarityAdd
BLAST

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni144 – 1485Substrate bindingBy similarity
Regioni211 – 2166Substrate bindingBy similarity
Regioni283 – 2842Substrate bindingBy similarity

Sequence similaritiesi

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0167.
HOGENOMiHOG000225103.
HOVERGENiHBG006898.
InParanoidiQ5E9W3.
KOiK00254.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00225. DHO_dh_type2.
InterProiIPR013785. Aldolase_TIM.
IPR012135. Dihydroorotate_DH_1_2.
IPR005719. Dihydroorotate_DH_2.
IPR001295. Dihydroorotate_DH_CS.
[Graphical view]
PfamiPF01180. DHO_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF000164. DHO_oxidase. 1 hit.
TIGRFAMsiTIGR01036. pyrD_sub2. 1 hit.
PROSITEiPS00911. DHODEHASE_1. 1 hit.
PS00912. DHODEHASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5E9W3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAWRQLKKRA QDAMVILGGG GLLFASYLTA TGDEHFYAEL LMPSLQRLLD
60 70 80 90 100
PETAHRLAVR FTSLGLLPRT TFQDSDMLEV RVLGHKFRNP VGIAAGFDKH
110 120 130 140 150
GEAVDGLYKM GFGFVEIGSV TPEPQEGNPR PRVFRLPEDQ AIINRYGFNS
160 170 180 190 200
HGLSVVEHRL RARQQTQARL TEDGLPLGIN LGKNKTSVDA ASDYAEGVRV
210 220 230 240 250
LGPLADYLVV NVSSPNTAGL RSLQGKAELR RLLTKVLQER DALKVAHKPA
260 270 280 290 300
VLVKIAPDLT AQDKEDIASV VRELGIDGLI VTNSTVSRPA SLQGALRSEP
310 320 330 340 350
GGLSGKPLRD LSTQTIREMY ALTQGRVPIV GVGGVSSGQD ALEKIRAGAS
360 370 380 390
LVQLYTALTY RGPPVVGGVK RELEALLKEQ GFARVTDAIG ADHRR
Length:395
Mass (Da):42,776
Last modified:March 15, 2005 - v1
Checksum:i9D093C1641A44BD0
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BT020807 mRNA. Translation: AAX08824.1.
BC120337 mRNA. Translation: AAI20338.1.
RefSeqiNP_001015650.1. NM_001015650.1.
UniGeneiBt.7483.

Genome annotation databases

GeneIDi533873.
KEGGibta:533873.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BT020807 mRNA. Translation: AAX08824.1 .
BC120337 mRNA. Translation: AAI20338.1 .
RefSeqi NP_001015650.1. NM_001015650.1.
UniGenei Bt.7483.

3D structure databases

ProteinModelPortali Q5E9W3.
SMRi Q5E9W3. Positions 30-395.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi Q5E9W3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 533873.
KEGGi bta:533873.

Organism-specific databases

CTDi 1723.

Phylogenomic databases

eggNOGi COG0167.
HOGENOMi HOG000225103.
HOVERGENi HBG006898.
InParanoidi Q5E9W3.
KOi K00254.

Enzyme and pathway databases

UniPathwayi UPA00070 ; UER00946 .
SABIO-RK Q5E9W3.

Miscellaneous databases

NextBioi 20876177.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00225. DHO_dh_type2.
InterProi IPR013785. Aldolase_TIM.
IPR012135. Dihydroorotate_DH_1_2.
IPR005719. Dihydroorotate_DH_2.
IPR001295. Dihydroorotate_DH_CS.
[Graphical view ]
Pfami PF01180. DHO_dh. 1 hit.
[Graphical view ]
PIRSFi PIRSF000164. DHO_oxidase. 1 hit.
TIGRFAMsi TIGR01036. pyrD_sub2. 1 hit.
PROSITEi PS00911. DHODEHASE_1. 1 hit.
PS00912. DHODEHASE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Thalamus.
  3. "Purification and properties of the bovine liver mitochondrial dihydroorotate dehydrogenase."
    Hines V., Keys L.D. III, Johnston M.
    J. Biol. Chem. 261:11386-11392(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: CATALYTIC ACTIVITY, SUBSTRATE SPECIFICITY, BIOPHYSICOCHEMICAL PROPERTIES.
  4. Erratum
    Hines V., Keys L.D. III, Johnston M.
    J. Biol. Chem. 262:15322-15322(1987)
  5. "Analysis of the kinetic mechanism of the bovine liver mitochondrial dihydroorotate dehydrogenase."
    Hines V., Johnston M.
    Biochemistry 28:1222-1226(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: BIOPHYSICOCHEMICAL PROPERTIES, ENZYME KINETICS.

Entry informationi

Entry nameiPYRD_BOVIN
AccessioniPrimary (citable) accession number: Q5E9W3
Secondary accession number(s): Q0P590
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 2, 2006
Last sequence update: March 15, 2005
Last modified: October 29, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3