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Protein

tRNA wybutosine-synthesizing protein 3 homolog

Gene

TYW3

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

Probable S-adenosyl-L-methionine-dependent methyltransferase that acts as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA (By similarity).By similarity

Catalytic activityi

S-adenosyl-L-methionine + 7-((3S)-(3-amino-3-carboxypropyl))-4-demethylwyosine(37) in tRNA(Phe) = S-adenosyl-L-homocysteine + 7-((3S)-(3-amino-3-carboxypropyl))wyosine(37) in tRNA(Phe).

Pathwayi: wybutosine-tRNA(Phe) biosynthesis

This protein is involved in the pathway wybutosine-tRNA(Phe) biosynthesis, which is part of tRNA modification.
View all proteins of this organism that are known to be involved in the pathway wybutosine-tRNA(Phe) biosynthesis and in tRNA modification.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00375.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA wybutosine-synthesizing protein 3 homolog (EC:2.1.1.282)
Short name:
tRNA-yW-synthesizing protein 3
Alternative name(s):
tRNA(Phe) 7-((3-amino-3-carboxypropyl)-4-demethylwyosine(37)-N(4))-methyltransferase
Gene namesi
Name:TYW3
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 3

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 258258tRNA wybutosine-synthesizing protein 3 homologPRO_0000281841Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei25 – 251PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ5E9U4.
PRIDEiQ5E9U4.

Interactioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000006849.

Structurei

3D structure databases

ProteinModelPortaliQ5E9U4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TYW3 family.Curated

Phylogenomic databases

eggNOGiKOG1228. Eukaryota.
COG1590. LUCA.
GeneTreeiENSGT00390000018149.
HOGENOMiHOG000007205.
HOVERGENiHBG106708.
InParanoidiQ5E9U4.
KOiK15450.
OMAiGKWHHYA.
OrthoDBiEOG76T9T0.
TreeFamiTF329327.

Family and domain databases

Gene3Di3.30.1960.10. 1 hit.
InterProiIPR003827. tRNA_yW-synthesising.
[Graphical view]
PfamiPF02676. TYW3. 1 hit.
[Graphical view]
SUPFAMiSSF111278. SSF111278. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5E9U4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDLSAEFKRW KAQCLSKADL SRKGSVDEDV LEIVQLLNGQ EQFFTTSSCA
60 70 80 90 100
GRIILLDRSV NGSEVQKQNC CWLLVTHKAC VKDDVIVALQ KAKGDAILKF
110 120 130 140 150
EPLVLHVQCR QLQDAQILHS VAIDSGFRNS GITVGKRGKT MLAVRSTHGL
160 170 180 190 200
EVPLSHQGKL MVTEEYINFL LKIANQKMEE NKKRIERFYH CLQHALEKET
210 220 230 240 250
VSTTSQPKEK VNTSYIRKKK RNPGKARGKR VNEEHDKELE NNDHDDPGIS

DTIFPEDY
Length:258
Mass (Da):29,303
Last modified:March 15, 2005 - v1
Checksum:iB0ABAB3BB79E095C
GO

Sequence cautioni

The sequence AAI14026.1 differs from that shown. Reason: Frameshift at position 221. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti170 – 1701L → Q in AAI14026 (Ref. 2) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT020826 mRNA. Translation: AAX08843.1.
BC114025 mRNA. Translation: AAI14026.1. Frameshift.
RefSeqiNP_001015620.1. NM_001015620.1.
UniGeneiBt.16963.

Genome annotation databases

EnsembliENSBTAT00000006849; ENSBTAP00000006849; ENSBTAG00000005196.
GeneIDi519731.
KEGGibta:519731.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT020826 mRNA. Translation: AAX08843.1.
BC114025 mRNA. Translation: AAI14026.1. Frameshift.
RefSeqiNP_001015620.1. NM_001015620.1.
UniGeneiBt.16963.

3D structure databases

ProteinModelPortaliQ5E9U4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000006849.

Proteomic databases

PaxDbiQ5E9U4.
PRIDEiQ5E9U4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000006849; ENSBTAP00000006849; ENSBTAG00000005196.
GeneIDi519731.
KEGGibta:519731.

Organism-specific databases

CTDi127253.

Phylogenomic databases

eggNOGiKOG1228. Eukaryota.
COG1590. LUCA.
GeneTreeiENSGT00390000018149.
HOGENOMiHOG000007205.
HOVERGENiHBG106708.
InParanoidiQ5E9U4.
KOiK15450.
OMAiGKWHHYA.
OrthoDBiEOG76T9T0.
TreeFamiTF329327.

Enzyme and pathway databases

UniPathwayiUPA00375.

Miscellaneous databases

NextBioi20872937.

Family and domain databases

Gene3Di3.30.1960.10. 1 hit.
InterProiIPR003827. tRNA_yW-synthesising.
[Graphical view]
PfamiPF02676. TYW3. 1 hit.
[Graphical view]
SUPFAMiSSF111278. SSF111278. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Testis.

Entry informationi

Entry nameiTYW3_BOVIN
AccessioniPrimary (citable) accession number: Q5E9U4
Secondary accession number(s): Q24K18
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: March 15, 2005
Last modified: November 11, 2015
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.