Q5E9T4 (TPK1_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thiamin pyrophosphokinase 1
EC=2.7.6.2

Alternative name(s):
Thiamine pyrophosphokinase 1

Gene names

Name:

TPK1

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	243 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the phosphorylation of thiamine to thiamine pyrophosphate. Can also catalyze the phosphorylation of pyrithiamine to pyrithiamine pyrophosphate By similarity.
Catalytic activity	ATP + thiamine = AMP + thiamine diphosphate.
Pathway	Cofactor biosynthesis; thiamine diphosphate biosynthesis; thiamine diphosphate from thiamine: step 1/1.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the thiamine pyrophosphokinase family.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	thiamine diphosphate biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW kinase activity Inferred from electronic annotation. Source: UniProtKB-KW thiamine diphosphokinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 243	243	Thiamin pyrophosphokinase 1		PRO_0000239117

Sequences

	Sequence		Length	Mass (Da)
	Q5E9T4 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: 00471681ACC6FB47	FASTA	243	27,028
	10 20 30 40 50 60 MEHAITPLDP LLPSGSLKYC LVILNQPLDK CFRHLWHKAL LRACADGGAN HLYDVTEGER 70 80 90 100 110 120 ESFLPEFISG DFDSIRPEVR EHYAIKGCEI ISTPDQDHTD FTKCLEVLQK KIEEKDLQVD 130 140 150 160 170 180 MIVTLGGLAG RFDQIMASVS TLFQAPQITS LPVIIIQEES LIYLLQPGKH KLHVDTGMEG 190 200 210 220 230 240 DWCGLIPVGQ PCNQVTTTGL KWNLTHQMLG FGTLVSTSNT YDGSGVVTVE TDHPLLWTMA IKN

References

[1]	"Characterization of 954 bovine full-CDS cDNA sequences." Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L. BMC Genomics 6:166-166(2005) [PubMed: 16305752] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[2]	NIH - Mammalian Gene Collection (MGC) project Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Basal ganglia.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BT020836 mRNA. Translation: AAX08853.1. BC119895 mRNA. Translation: AAI19896.1.
IPI	IPI00692460.
RefSeq	NP_001073839.1. NM_001080370.1.
UniGene	Bt.6512.
3D structure databases
ProteinModelPortal	Q5E9T4.
SMR	Q5E9T4. Positions 1-243.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5E9T4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000023510; ENSBTAP00000023510; ENSBTAG00000017676. ENSBTAT00000044036; ENSBTAP00000041553; ENSBTAG00000017676.
GeneID	788066.
KEGG	bta:788066.
Organism-specific databases
CTD	27010.
Phylogenomic databases
eggNOG	maNOG12515.
GeneTree	ENSGT00390000016016.
HOVERGEN	HBG003568.
InParanoid	Q5E9T4.
OMA	NQPLDKC.
OrthoDB	EOG4DJJX5.
PhylomeDB	Q5E9T4.
Family and domain databases
InterPro	IPR006282. Thi_PPkinase. IPR016966. Thiamin_pyrophosphokinase_euk. IPR007373. Thiamin_PyroPKinase_B1-bd. IPR007371. TPK_catalytic. [Graphical view]
Gene3D	G3DSA:2.60.120.320. G3DSA:2.60.120.320. 1 hit. G3DSA:3.40.50.10240. TPK_catalytic. 1 hit.
KO	K00949.
Pfam	PF04265. TPK_B1_binding. 1 hit. PF04263. TPK_catalytic. 1 hit. [Graphical view]
PIRSF	PIRSF031057. Thiamin_pyrophosphokinase. 1 hit.
SMART	SM00983. TPK_B1_binding. 1 hit. [Graphical view]
SUPFAM	SSF63862. TPK_B1_bd. 1 hit. SSF63999. TPK_catalytic. 1 hit.
TIGRFAMs	TIGR01378. Thi_PPkinase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TPK1_BOVIN

Accession

Primary (citable) accession number: Q5E9T4
Secondary accession number(s): Q0P5L2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	March 15, 2005
Last modified:	January 25, 2012
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program