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Protein

Thiamin pyrophosphokinase 1

Gene

TPK1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the phosphorylation of thiamine to thiamine pyrophosphate. Can also catalyze the phosphorylation of pyrithiamine to pyrithiamine pyrophosphate (By similarity).By similarity

Catalytic activityi

ATP + thiamine = AMP + thiamine diphosphate.

Pathwayi

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. kinase activity Source: UniProtKB-KW
  3. thiamine binding Source: InterPro
  4. thiamine diphosphokinase activity Source: UniProtKB-EC

GO - Biological processi

  1. thiamine diphosphate biosynthetic process Source: UniProtKB-UniPathway
  2. thiamine metabolic process Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_206123. Vitamin B1 (thiamin) metabolism.
UniPathwayiUPA00060; UER00597.

Names & Taxonomyi

Protein namesi
Recommended name:
Thiamin pyrophosphokinase 1 (EC:2.7.6.2)
Alternative name(s):
Thiamine pyrophosphokinase 1
Gene namesi
Name:TPK1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome 4

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 243243Thiamin pyrophosphokinase 1PRO_0000239117Add
BLAST

Proteomic databases

PRIDEiQ5E9T4.

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ5E9T4.
SMRiQ5E9T4. Positions 1-243.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thiamine pyrophosphokinase family.Curated

Phylogenomic databases

eggNOGiCOG1564.
GeneTreeiENSGT00390000016016.
HOGENOMiHOG000180834.
HOVERGENiHBG003568.
InParanoidiQ5E9T4.
KOiK00949.
OMAiNYFRHLW.
OrthoDBiEOG76HQ2J.
TreeFamiTF313224.

Family and domain databases

Gene3Di2.60.120.320. 1 hit.
3.40.50.10240. 1 hit.
InterProiIPR006282. Thi_PPkinase.
IPR016966. Thiamin_pyrophosphokinase_euk.
IPR007373. Thiamin_PyroPKinase_B1-bd.
IPR007371. TPK_catalytic.
[Graphical view]
PfamiPF04265. TPK_B1_binding. 1 hit.
PF04263. TPK_catalytic. 1 hit.
[Graphical view]
PIRSFiPIRSF031057. Thiamin_pyrophosphokinase. 1 hit.
SMARTiSM00983. TPK_B1_binding. 1 hit.
[Graphical view]
SUPFAMiSSF63862. SSF63862. 1 hit.
SSF63999. SSF63999. 1 hit.
TIGRFAMsiTIGR01378. thi_PPkinase. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5E9T4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEHAITPLDP LLPSGSLKYC LVILNQPLDK CFRHLWHKAL LRACADGGAN
60 70 80 90 100
HLYDVTEGER ESFLPEFISG DFDSIRPEVR EHYAIKGCEI ISTPDQDHTD
110 120 130 140 150
FTKCLEVLQK KIEEKDLQVD MIVTLGGLAG RFDQIMASVS TLFQAPQITS
160 170 180 190 200
LPVIIIQEES LIYLLQPGKH KLHVDTGMEG DWCGLIPVGQ PCNQVTTTGL
210 220 230 240
KWNLTHQMLG FGTLVSTSNT YDGSGVVTVE TDHPLLWTMA IKN
Length:243
Mass (Da):27,028
Last modified:March 15, 2005 - v1
Checksum:i00471681ACC6FB47
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT020836 mRNA. Translation: AAX08853.1.
BC119895 mRNA. Translation: AAI19896.1.
RefSeqiNP_001073839.1. NM_001080370.1.
XP_005205956.1. XM_005205899.1.
UniGeneiBt.6512.

Genome annotation databases

EnsembliENSBTAT00000023510; ENSBTAP00000023510; ENSBTAG00000017676.
ENSBTAT00000044036; ENSBTAP00000041553; ENSBTAG00000017676.
GeneIDi788066.
KEGGibta:788066.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT020836 mRNA. Translation: AAX08853.1.
BC119895 mRNA. Translation: AAI19896.1.
RefSeqiNP_001073839.1. NM_001080370.1.
XP_005205956.1. XM_005205899.1.
UniGeneiBt.6512.

3D structure databases

ProteinModelPortaliQ5E9T4.
SMRiQ5E9T4. Positions 1-243.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ5E9T4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000023510; ENSBTAP00000023510; ENSBTAG00000017676.
ENSBTAT00000044036; ENSBTAP00000041553; ENSBTAG00000017676.
GeneIDi788066.
KEGGibta:788066.

Organism-specific databases

CTDi27010.

Phylogenomic databases

eggNOGiCOG1564.
GeneTreeiENSGT00390000016016.
HOGENOMiHOG000180834.
HOVERGENiHBG003568.
InParanoidiQ5E9T4.
KOiK00949.
OMAiNYFRHLW.
OrthoDBiEOG76HQ2J.
TreeFamiTF313224.

Enzyme and pathway databases

UniPathwayiUPA00060; UER00597.
ReactomeiREACT_206123. Vitamin B1 (thiamin) metabolism.

Miscellaneous databases

NextBioi20928859.

Family and domain databases

Gene3Di2.60.120.320. 1 hit.
3.40.50.10240. 1 hit.
InterProiIPR006282. Thi_PPkinase.
IPR016966. Thiamin_pyrophosphokinase_euk.
IPR007373. Thiamin_PyroPKinase_B1-bd.
IPR007371. TPK_catalytic.
[Graphical view]
PfamiPF04265. TPK_B1_binding. 1 hit.
PF04263. TPK_catalytic. 1 hit.
[Graphical view]
PIRSFiPIRSF031057. Thiamin_pyrophosphokinase. 1 hit.
SMARTiSM00983. TPK_B1_binding. 1 hit.
[Graphical view]
SUPFAMiSSF63862. SSF63862. 1 hit.
SSF63999. SSF63999. 1 hit.
TIGRFAMsiTIGR01378. thi_PPkinase. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Basal ganglia.

Entry informationi

Entry nameiTPK1_BOVIN
AccessioniPrimary (citable) accession number: Q5E9T4
Secondary accession number(s): Q0P5L2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: March 15, 2005
Last modified: January 7, 2015
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.