Reviewed, UniProtKB/Swiss-Prot Q5E9R2 (PLCD_BOVIN)

Names and origin

Protein names

Recommended name:
    1-acyl-sn-glycerol-3-phosphate acyltransferase delta
    EC=2.3.1.51
Alternative name(s):
    1-acylglycerol-3-phosphate O-acyltransferase 4
      Short name=1-AGP acyltransferase 4
      Short name=1-AGPAT 4
    Lysophosphatidic acid acyltransferase delta
      Short name=LPAAT-delta

Gene names

Name:

AGPAT4

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	378 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Converts lysophosphatidic acid (LPA) into phosphatidic acid by incorporating an acyl moiety at the sn-2 position of the glycerol backbone By similarity.
Catalytic activity	Acyl-CoA + 1-acyl-sn-glycerol 3-phosphate = CoA + 1,2-diacyl-sn-glycerol 3-phosphate.
Pathway	Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 2/3.
Subcellular location	Membrane; Multi-pass membrane protein Potential.
Domain	The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate By similarity.
Sequence similarities	Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Membrane
Domain	Transmembrane
Molecular function	Acyltransferase Transferase
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	1-acylglycerol-3-phosphate O-acyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 378	378	1-acyl-sn-glycerol-3-phosphate acyltransferase delta		PRO_0000239116
Regions
	Transmembrane	11 – 31	21	Potential
	Transmembrane	125 – 145	21	Potential
	Transmembrane	307 – 327	21	Potential
	Transmembrane	338 – 358	21	Potential
	Motif	96 – 101	6	HXXXXD motif
Experimental info
	Sequence conflict	290	1	R → H in AAX08708. Ref.1
	Sequence conflict	327	1	I → V in AAX08708. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q5E9R2-1 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: 2E7903D835D7F0A5	FASTA	378	43,907
	10 20 30 40 50 60 MDLVALLKSH FLCHLIFCYV FIVSGLIINT IQLCTLLLWP VNKQLFRKIN CRLSYCVSSQ 70 80 90 100 110 120 LVMLLEWWSG TECVIYTDPR AYPKYGKENA IVVLNHKFEI DFLCGWSLAE RFGVLGGSKV 130 140 150 160 170 180 LAKKELAYVP IIGWMWYFTE MVFCTRKWEQ DRKTVSESLL HLRDYPEKYF FLIHCEGTRF 190 200 210 220 230 240 TEKKHQISMQ VAQAKGLPSL KHHLLPRTKG FAVTVRSLRN VVSAVYDCTL NFRNNENPTL 250 260 270 280 290 300 LGVLNGKKYH ADLYVRRIPL EEVPEEEDKC AAWLHKLYQE KDAFQEEYSR TGTFPETPVV 310 320 330 340 350 360 PPRRPWTLVN WLFWASMLLY PFFRFVINMV SSGSSLTLAS FVLVFFVASM GVRWMIGVTE 370 IDKGSAYGNM DSKQKHSD

References

[1]	"Characterization of 954 bovine full-CDS cDNA sequences." Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L. BMC Genomics 6:166-166(2005) [PubMed: 16305752] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BT020691 mRNA. Translation: AAX08708.1. BT020858 mRNA. Translation: AAX08875.1.
IPI	IPI00694418.
UniGene	Bt.25871
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q5E9R2.
Genome annotation databases
Ensembl	ENSBTAT00000010089; ENSBTAP00000010089; ENSBTAG00000007668; Bos taurus. [Genome view]
Phylogenomic databases
HOVERGEN	Q5E9R2.
Enzyme and pathway databases
BRENDA	2.3.1.51. 251.
Family and domain databases
InterPro	IPR002123. Acyltransferase. [Graphical view]
Pfam	PF01553. Acyltransferase. 1 hit. [Graphical view]
SMART	SM00563. PlsC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PLCD_BOVIN

Accession

Primary (citable) accession number: Q5E9R2
Secondary accession number(s): Q5EA78

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	March 15, 2005
Last modified:	October 13, 2009
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)