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Protein

Sphingosine 1-phosphate receptor 1

Gene

S1PR1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

G-protein coupled receptor for the bioactive lysosphingolipid sphingosine 1-phosphate (S1P) that seems to be coupled to the G(i) subclass of heteromeric G proteins. Signaling leads to the activation of RAC1, SRC, PTK2/FAK1 and MAP kinases. Plays an important role in cell migration, probably via its role in the reorganization of the actin cytoskeleton and the formation of lamellipodia in response to stimuli that increase the activity of the sphingosine kinase SPHK1. Required for normal chemotaxis toward sphingosine 1-phosphate. Required for normal embryonic heart development and normal cardiac morphogenesis. Plays an important role in the regulation of sprouting angiogenesis and vascular maturation. Inhibits sprouting angiogenesis to prevent excessive sprouting during blood vessel development. Required for normal egress of mature T-cells from the thymus into the blood stream and into peripheral lymphoid organs. Plays a role in the migration of osteoclast precursor cells, the regulation of bone mineralization and bone homeostasis. Plays a role in responses to oxidized 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine by pulmonary endothelial cells and in the protection against ventilator-induced lung injury (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Keywords - Biological processi

Angiogenesis, Chemotaxis

Enzyme and pathway databases

ReactomeiR-BTA-418594. G alpha (i) signalling events.
R-BTA-419408. Lysosphingolipid and LPA receptors.

Names & Taxonomyi

Protein namesi
Recommended name:
Sphingosine 1-phosphate receptor 1
Short name:
S1P receptor 1
Short name:
S1P1
Alternative name(s):
Endothelial differentiation G-protein coupled receptor 1
Sphingosine 1-phosphate receptor Edg-1
Short name:
S1P receptor Edg-1
CD_antigen: CD363
Gene namesi
Name:S1PR1
Synonyms:EDG1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 3

Subcellular locationi

  • Cell membrane By similarity; Multi-pass membrane protein By similarity
  • Endosome By similarity
  • Membrane raft By similarity

  • Note: Recruited to caveolin-enriched plasma membrane microdomains in response to oxidized 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine. Ligand binding leads to receptor internalization (By similarity).By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini2 – 46ExtracellularBy similarityAdd BLAST45
Transmembranei47 – 68Helical; Name=1By similarityAdd BLAST22
Topological domaini69 – 82CytoplasmicBy similarityAdd BLAST14
Transmembranei83 – 104Helical; Name=2By similarityAdd BLAST22
Topological domaini105 – 116ExtracellularBy similarityAdd BLAST12
Transmembranei117 – 138Helical; Name=3By similarityAdd BLAST22
Topological domaini139 – 160CytoplasmicBy similarityAdd BLAST22
Transmembranei161 – 182Helical; Name=4By similarityAdd BLAST22
Topological domaini183 – 196ExtracellularBy similarityAdd BLAST14
Transmembranei197 – 224Helical; Name=5By similarityAdd BLAST28
Topological domaini225 – 257CytoplasmicBy similarityAdd BLAST33
Transmembranei258 – 278Helical; Name=6By similarityAdd BLAST21
Topological domaini279 – 289ExtracellularBy similarityAdd BLAST11
Transmembranei290 – 310Helical; Name=7By similarityAdd BLAST21
Topological domaini311 – 382CytoplasmicBy similarityAdd BLAST72

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002446212 – 382Sphingosine 1-phosphate receptor 1Add BLAST381

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei10N6-acetyllysineBy similarity1
Glycosylationi30N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi184 ↔ 191PROSITE-ProRule annotation
Modified residuei236Phosphothreonine; by PKB/AKT1By similarity1
Disulfide bondi282 ↔ 287PROSITE-ProRule annotation
Lipidationi328S-palmitoyl cysteineBy similarity1
Modified residuei351PhosphoserineBy similarity1
Modified residuei353PhosphoserineSequence analysis1

Post-translational modificationi

S1P-induced endothelial cell migration requires the PKB/AKT1-mediated phosphorylation of the third intracellular loop at the Thr-236 residue.By similarity

Keywords - PTMi

Acetylation, Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiQ5E9P3.
PRIDEiQ5E9P3.

Expressioni

Gene expression databases

BgeeiENSBTAG00000005990.

Interactioni

Subunit structurei

Interacts with GNAI1 and GNAI3.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000007861.

Structurei

3D structure databases

ProteinModelPortaliQ5E9P3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni120 – 121Sphingosine 1-phosphate bindingBy similarity2
Regioni265 – 269Sphingosine 1-phosphate bindingBy similarity5

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IIA7. Eukaryota.
ENOG410XQD3. LUCA.
GeneTreeiENSGT00760000118804.
HOGENOMiHOG000233501.
HOVERGENiHBG103071.
InParanoidiQ5E9P3.
KOiK04288.
OMAiIGGMEFS.
OrthoDBiEOG091G0DD4.
TreeFamiTF330052.

Family and domain databases

InterProiIPR000987. EDG1_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR004061. S1P_rcpt.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00642. EDG1RECEPTOR.
PR00237. GPCRRHODOPSN.
PR01523. S1PRECEPTOR.
SMARTiSM01381. 7TM_GPCR_Srsx. 1 hit.
[Graphical view]
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5E9P3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSTRIPLVK ALHSPVSDYV NYDIIVRHYN YTGKLKISAD KDNGIKLISV
60 70 80 90 100
VFILICCFII LENIFVLLTI WKTKKFHRPM YYFIGNLALS DLLAGVAYTA
110 120 130 140 150
NLLLSGATTY KLTPAQWFLR EGSMFVALSA SVFSLLAIAI ERYITMLKMK
160 170 180 190 200
LHNGSNRFRS FLLISACWVI SLILGGLPIM GWNCISTLPS CSTVLPLYHK
210 220 230 240 250
HYILFCTTVF TLLLLSIVIL YCRIYSLVRT RSRRLTFRKN ISKASRSSEK
260 270 280 290 300
SLALLKTVII VLGVFIACWA PLFILLLLDV GCKVKTCDIL FRTEYFLVLA
310 320 330 340 350
VLNSGTNPII YTLSNKEMRR AFVRIMSCCK CPSRDSASKF TRPIIAGMEF
360 370 380
SRSKSDNSSH PQKDDGDNPE TIMSSGNVNS SS
Length:382
Mass (Da):42,949
Last modified:March 15, 2005 - v1
Checksum:i81C19138BDFB8880
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT020877 mRNA. Translation: AAX08894.1.
BC114045 mRNA. Translation: AAI14046.1.
RefSeqiNP_001013603.2. NM_001013585.4.
UniGeneiBt.26265.

Genome annotation databases

EnsembliENSBTAT00000007861; ENSBTAP00000007861; ENSBTAG00000005990.
GeneIDi281135.
KEGGibta:281135.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT020877 mRNA. Translation: AAX08894.1.
BC114045 mRNA. Translation: AAI14046.1.
RefSeqiNP_001013603.2. NM_001013585.4.
UniGeneiBt.26265.

3D structure databases

ProteinModelPortaliQ5E9P3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000007861.

Protein family/group databases

GPCRDBiSearch...

Proteomic databases

PaxDbiQ5E9P3.
PRIDEiQ5E9P3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000007861; ENSBTAP00000007861; ENSBTAG00000005990.
GeneIDi281135.
KEGGibta:281135.

Organism-specific databases

CTDi1901.

Phylogenomic databases

eggNOGiENOG410IIA7. Eukaryota.
ENOG410XQD3. LUCA.
GeneTreeiENSGT00760000118804.
HOGENOMiHOG000233501.
HOVERGENiHBG103071.
InParanoidiQ5E9P3.
KOiK04288.
OMAiIGGMEFS.
OrthoDBiEOG091G0DD4.
TreeFamiTF330052.

Enzyme and pathway databases

ReactomeiR-BTA-418594. G alpha (i) signalling events.
R-BTA-419408. Lysosphingolipid and LPA receptors.

Gene expression databases

BgeeiENSBTAG00000005990.

Family and domain databases

InterProiIPR000987. EDG1_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR004061. S1P_rcpt.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00642. EDG1RECEPTOR.
PR00237. GPCRRHODOPSN.
PR01523. S1PRECEPTOR.
SMARTiSM01381. 7TM_GPCR_Srsx. 1 hit.
[Graphical view]
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiS1PR1_BOVIN
AccessioniPrimary (citable) accession number: Q5E9P3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: March 15, 2005
Last modified: November 2, 2016
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.