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Protein

Protein arginine N-methyltransferase 6

Gene

PRMT6

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Arginine methyltransferase that can catalyze the formation of both omega-N monomethylarginine (MMA) and asymmetrical dimethylarginine (aDMA), with a strong preference for the formation of aDMA. Preferentially methylates arginyl residues present in a glycine and arginine-rich domain and displays preference for monomethylated substrates. Specifically mediates the asymmetric dimethylation of histone H3 'Arg-2' to form H3R2me2a. H3R2me2a represents a specific tag for epigenetic transcriptional repression and is mutually exclusive with methylation on histone H3 'Lys-4' (H3K4me2 and H3K4me3). Acts as a transcriptional repressor of various genes such as HOXA2, THBS1 and TP53 (By similarity). Repression of TP53 blocks cellular senescence (By similarity). Also methylates histone H2A and H4 'Arg-3' (H2AR3me and H4R3me, respectively). Acts as a regulator of DNA base excision during DNA repair by mediating the methylation of DNA polymerase beta (POLB), leading to the stimulation of its polymerase activity by enhancing DNA binding and processivity. Methylates HMGA1. Regulates alternative splicing events. Acts as a transcriptional coactivator of a number of steroid hormone receptors including ESR1, ESR2, PGR and NR3C1. Promotes fasting-induced transcriptional activation of the gluconeogenic program through methylation of the CRTC2 transcription coactivator. Methylates GPS2, protecting GPS2 from ubiquitination and degradation (By similarity).By similarity

Catalytic activityi

2 S-adenosyl-L-methionine + [protein]-L-arginine = 2 S-adenosyl-L-homocysteine + [protein]-N(omega),N(omega)-dimethyl-L-arginine.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei57S-adenosyl-L-methionineBy similarity1
Binding sitei66S-adenosyl-L-methionineBy similarity1
Binding sitei90S-adenosyl-L-methionine; via carbonyl oxygenBy similarity1
Binding sitei112S-adenosyl-L-methionineBy similarity1
Binding sitei141S-adenosyl-L-methionineBy similarity1
Active sitei155By similarity1
Active sitei164By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChromatin regulator, Methyltransferase, Repressor, Transferase
Biological processDNA damage, DNA repair, Transcription, Transcription regulation
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

ReactomeiR-BTA-3214858 RMTs methylate histone arginines
R-BTA-8936459 RUNX1 regulates genes involved in megakaryocyte differentiation and platelet function

Names & Taxonomyi

Protein namesi
Recommended name:
Protein arginine N-methyltransferase 6 (EC:2.1.1.319By similarity)
Alternative name(s):
Histone-arginine N-methyltransferase PRMT6
Gene namesi
Name:PRMT6
Synonyms:HRMT1L6
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 3

Organism-specific databases

VGNCiVGNC:33351 PRMT6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002123311 – 375Protein arginine N-methyltransferase 6Add BLAST375

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei21PhosphothreonineBy similarity1
Modified residuei38Omega-N-methylated arginine; by autocatalysisBy similarity1

Post-translational modificationi

Automethylation enhances its stability.By similarity

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

PaxDbiQ5E9L5
PRIDEiQ5E9L5

Expressioni

Gene expression databases

BgeeiENSBTAG00000039951

Interactioni

Subunit structurei

Interacts with (and methylates) HIV-1 Tat, Rev and Nucleocapsid protein p7 (NC). Interacts with EPB41L3 and NCOA1.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000009750

Structurei

3D structure databases

ProteinModelPortaliQ5E9L5
SMRiQ5E9L5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini44 – 374SAM-dependent MTase PRMT-typePROSITE-ProRule annotationAdd BLAST331

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. Protein arginine N-methyltransferase family. PRMT6 subfamily.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1499 Eukaryota
ENOG410XQYH LUCA
GeneTreeiENSGT00550000074406
HOGENOMiHOG000198521
HOVERGENiHBG095907
InParanoidiQ5E9L5
KOiK11437
OMAiGVVFHWM
OrthoDBiEOG091G0A0U
TreeFamiTF328817

Family and domain databases

InterProiView protein in InterPro
IPR025799 Arg_MeTrfase
IPR029063 SAM-dependent_MTases
SUPFAMiSSF53335 SSF53335, 1 hit
PROSITEiView protein in PROSITE
PS51678 SAM_MT_PRMT, 1 hit

Sequencei

Sequence statusi: Complete.

Q5E9L5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQPKRRKLE SGGGGEGGEG TEEEDGGELE VAVPRPRRTR RERDQLYYQC
60 70 80 90 100
YSDVSVHEEM IADRVRTDAY RLGILRNWAA LRGKTVLDVG AGTGILSIFC
110 120 130 140 150
AQAGARRVYA VEASDIWQQA REVVRLNGLE DRVHVLPGPV ETVELPEQVD
160 170 180 190 200
AIVSEWMGCG LLHESMLSSV LHARTKWLKE GGLLLPASAE LFVAPISDQM
210 220 230 240 250
LELRLSFWSQ MKQLYGVDMS CLESFATRCL MGHSEIVVQG LSGEDVLARP
260 270 280 290 300
QCFARLELAR AGLEQELEAG VGGRFRFSCY GSAPMHGFAI WFQVTFPGGD
310 320 330 340 350
SEKPVVLSTS PFHPVTHWKQ ALLYLNEPVQ VEQDTDVSGE ITLLPSQDHH
360 370
RHLRVLLRYK VGDQEEKTKD FAMED
Length:375
Mass (Da):41,868
Last modified:March 15, 2005 - v1
Checksum:i234A46591A0428C5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT020905 mRNA Translation: AAX08922.1
RefSeqiNP_001014962.1, NM_001014962.1
UniGeneiBt.28284
Bt.65048

Genome annotation databases

EnsembliENSBTAT00000009750; ENSBTAP00000009750; ENSBTAG00000039951
GeneIDi540228
KEGGibta:540228

Similar proteinsi

Entry informationi

Entry nameiANM6_BOVIN
AccessioniPrimary (citable) accession number: Q5E9L5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: March 15, 2005
Last modified: May 23, 2018
This is version 89 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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