Reviewed, UniProtKB/Swiss-Prot Q5E9L5 (ANM6_BOVIN)

Names and origin

Protein names

Recommended name:
    Protein arginine N-methyltransferase 6
    EC=2.1.1.-
Alternative name(s):
    Histone-arginine N-methyltransferase PRMT6
    EC=2.1.1.125

Gene names

Name:	PRMT6
Synonyms:	HRMT1L6

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	375 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and asymmetrical dimethylarginine (aDMA), with a strong preference for the formation of aDMA. Preferentially methylates arginyl residues present in a glycine and arginine-rich domain and displays preference for monomethylated substrates. Specifically mediates the asymmetric dimethylation of histone H3 'Arg-2' to form H3R2me2a. H3R2me2a represents a specific tag for epigenetic transcriptional repression and is mutually exclusive with methylation on histone H3 'Lys-4' (H3K4me2 and H3K4me3). It thereby acts as a transcription corepressor of various genes such as HOXA2. Also methylates histone H2A and H4 'Arg-3' (H2AR3me and H4R3me, respectively). Acts as a regulator of DNA base excision during DNA repair by mediating the methylation of DNA polymerase beta (POLB), leading to stimulate the polymerase activity by enhancing DNA binding and processivity. Methylates HMGA1 By similarity.
Catalytic activity	S-adenosyl-L-methionine + histone-arginine = S-adenosyl-L-homocysteine + histone-N(omega)-methyl-arginine.
Subcellular location	Nucleus By similarity.
Post-translational modification	Automethylated By similarity.
Sequence similarities	Belongs to the protein arginine N-methyltransferase family. PRMT6 subfamily.

Ontologies

Keywords
Biological process	DNA damage DNA repair Transcription Transcription regulation
Cellular component	Nucleus
Ligand	S-adenosyl-L-methionine
Molecular function	Chromatin regulator Methyltransferase Transferase
PTM	Methylation Phosphoprotein
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW histone H3-R2 methylation Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of transcription, DNA-dependent Inferred from sequence or structural similarity. Source: UniProtKB peptidyl-arginine methylation, to asymmetrical-dimethyl arginine Inferred from sequence or structural similarity. Source: UniProtKB transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	histone binding Inferred from sequence or structural similarity. Source: UniProtKB histone methyltransferase activity (H2A-R3 specific) Inferred from sequence or structural similarity. Source: UniProtKB histone methyltransferase activity (H3-R2 specific) Inferred from sequence or structural similarity. Source: UniProtKB histone methyltransferase activity (H4-R3 specific) Inferred from sequence or structural similarity. Source: UniProtKB protein-arginine omega-N asymmetric methyltransferase activity Inferred from sequence or structural similarity. Source: UniProtKB protein-arginine omega-N monomethyltransferase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 375	375	Protein arginine N-methyltransferase 6		PRO_0000212331
Sites
	Binding site	57	1	S-adenosyl-L-methionine By similarity
	Binding site	66	1	S-adenosyl-L-methionine By similarity
	Binding site	90	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	112	1	S-adenosyl-L-methionine By similarity
	Binding site	141	1	S-adenosyl-L-methionine By similarity
Amino acid modifications
	Modified residue	21	1	Phosphothreonine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5E9L5-1 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: 234A46591A0428C5	FASTA	375	41,868
	10 20 30 40 50 60 MSQPKRRKLE SGGGGEGGEG TEEEDGGELE VAVPRPRRTR RERDQLYYQC YSDVSVHEEM 70 80 90 100 110 120 IADRVRTDAY RLGILRNWAA LRGKTVLDVG AGTGILSIFC AQAGARRVYA VEASDIWQQA 130 140 150 160 170 180 REVVRLNGLE DRVHVLPGPV ETVELPEQVD AIVSEWMGCG LLHESMLSSV LHARTKWLKE 190 200 210 220 230 240 GGLLLPASAE LFVAPISDQM LELRLSFWSQ MKQLYGVDMS CLESFATRCL MGHSEIVVQG 250 260 270 280 290 300 LSGEDVLARP QCFARLELAR AGLEQELEAG VGGRFRFSCY GSAPMHGFAI WFQVTFPGGD 310 320 330 340 350 360 SEKPVVLSTS PFHPVTHWKQ ALLYLNEPVQ VEQDTDVSGE ITLLPSQDHH RHLRVLLRYK 370 VGDQEEKTKD FAMED

References

[1]	"Characterization of 954 bovine full-CDS cDNA sequences." Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L. BMC Genomics 6:166-166(2005) [PubMed: 16305752] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BT020905 mRNA. Translation: AAX08922.1.
IPI	IPI00692823.
RefSeq	NP_001014962.1.
UniGene	Bt.28284 Bt.65048
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000009750; ENSBTAP00000009750; ENSBTAG00000039951; Bos taurus. [Genome view]
GeneID	540228.
KEGG	bta:540228.
Organism-specific databases
CTD	540228.
Phylogenomic databases
HOVERGEN	Q5E9L5.
OMA	GRFRFSC.
Family and domain databases
InterPro	IPR013216. Methyltransf_11. [Graphical view]
Pfam	PF08241. Methyltransf_11. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ANM6_BOVIN

Accession

Primary (citable) accession number: Q5E9L5

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	March 15, 2005
Last modified:	November 3, 2009
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)