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Q5E9K3

- PNPO_BOVIN

UniProt

Q5E9K3 - PNPO_BOVIN

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Protein

Pyridoxine-5'-phosphate oxidase

Gene

PNPO

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Catalyzes the oxidation of either pyridoxine 5'-phosphate (PNP) or pyridoxamine 5'-phosphate (PMP) into pyridoxal 5'-phosphate (PLP).By similarity

Catalytic activityi

Pyridoxamine 5'-phosphate + H2O + O2 = pyridoxal 5'-phosphate + NH3 + H2O2.
Pyridoxine 5'-phosphate + O2 = pyridoxal 5'-phosphate + H2O2.

Cofactori

FMNBy similarityNote: Binds 1 FMN per subunit.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei95 – 951FMNBy similarity
Binding sitei98 – 981FMN; via amide nitrogenBy similarity
Binding sitei100 – 1001SubstrateBy similarity
Binding sitei117 – 1171FMNBy similarity
Binding sitei157 – 1571SubstrateBy similarity
Binding sitei161 – 1611SubstrateBy similarity
Binding sitei165 – 1651SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi110 – 1112FMNBy similarity
Nucleotide bindingi174 – 1752FMNBy similarity

GO - Molecular functioni

  1. FMN binding Source: InterPro
  2. pyridoxamine-phosphate oxidase activity Source: UniProtKB-EC

GO - Biological processi

  1. pyridoxine biosynthetic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Pyridoxine biosynthesis

Keywords - Ligandi

Flavoprotein, FMN, Pyridoxal phosphate

Enzyme and pathway databases

ReactomeiREACT_213534. Vitamins B6 activation to pyridoxal phosphate.
UniPathwayiUPA00190; UER00304.
UPA00190; UER00305.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyridoxine-5'-phosphate oxidase (EC:1.4.3.5)
Alternative name(s):
Pyridoxamine-phosphate oxidase
Gene namesi
Name:PNPO
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome 19

Subcellular locationi

GO - Cellular componenti

  1. extracellular vesicular exosome Source: Ensembl
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 261261Pyridoxine-5'-phosphate oxidasePRO_0000167782Add
BLAST

Proteomic databases

PRIDEiQ5E9K3.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000016263.

Structurei

3D structure databases

ProteinModelPortaliQ5E9K3.
SMRiQ5E9K3. Positions 49-261.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni225 – 2273Substrate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0259.
GeneTreeiENSGT00390000011219.
HOGENOMiHOG000242755.
HOVERGENiHBG045634.
InParanoidiQ5E9K3.
KOiK00275.
OMAiELDHRGF.
OrthoDBiEOG7B8S4P.
TreeFamiTF313411.

Family and domain databases

Gene3Di2.30.110.10. 1 hit.
HAMAPiMF_01629. PdxH.
InterProiIPR000659. Pyridox_Oxase.
IPR019740. Pyridox_Oxase_CS.
IPR011576. Pyridox_Oxase_FMN-bd.
IPR019576. Pyridoxamine_oxidase_dimer_C.
IPR012349. Split_barrel_FMN-bd.
[Graphical view]
PANTHERiPTHR10851. PTHR10851. 1 hit.
PfamiPF10590. PNPOx_C. 1 hit.
PF01243. Pyridox_oxidase. 1 hit.
[Graphical view]
PIRSFiPIRSF000190. Pyd_amn-ph_oxd. 1 hit.
SUPFAMiSSF50475. SSF50475. 1 hit.
TIGRFAMsiTIGR00558. pdxH. 1 hit.
PROSITEiPS01064. PYRIDOX_OXIDASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5E9K3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MIFWLRSVIV TFGRPAEWPR CLRHLCSRGA AMDLGPMRKT YRGDPEAFEE
60 70 80 90 100
THLTSLDPVK QFAAWFEEAV QCPDIMEANA MCLATCTRDG KPSARMVLLK
110 120 130 140 150
GFGKDGFRFF TNFESRKGKE LDSNPFASLV FYWEPLHRQV RVEGPVKKLP
160 170 180 190 200
EEEAECYFHS RPKSSQIGAV VSHQSSVIPD REYLRKKNKE LEQLYQEQEV
210 220 230 240 250
PKPKYWGGYI LYPQVMEFWQ GQTNRLHDRI VFRRGLLTGD SPLGPMTHRG
260
EEDWVYERLA P
Length:261
Mass (Da):30,366
Last modified:March 15, 2005 - v1
Checksum:i7BDBB5478C6704AD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT020917 mRNA. Translation: AAX08934.1.
BC103208 mRNA. Translation: AAI03209.1.
RefSeqiNP_001014907.1. NM_001014907.1.
UniGeneiBt.24214.

Genome annotation databases

EnsembliENSBTAT00000016263; ENSBTAP00000016263; ENSBTAG00000012259.
GeneIDi512573.
KEGGibta:512573.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT020917 mRNA. Translation: AAX08934.1 .
BC103208 mRNA. Translation: AAI03209.1 .
RefSeqi NP_001014907.1. NM_001014907.1.
UniGenei Bt.24214.

3D structure databases

ProteinModelPortali Q5E9K3.
SMRi Q5E9K3. Positions 49-261.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9913.ENSBTAP00000016263.

Proteomic databases

PRIDEi Q5E9K3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSBTAT00000016263 ; ENSBTAP00000016263 ; ENSBTAG00000012259 .
GeneIDi 512573.
KEGGi bta:512573.

Organism-specific databases

CTDi 55163.

Phylogenomic databases

eggNOGi COG0259.
GeneTreei ENSGT00390000011219.
HOGENOMi HOG000242755.
HOVERGENi HBG045634.
InParanoidi Q5E9K3.
KOi K00275.
OMAi ELDHRGF.
OrthoDBi EOG7B8S4P.
TreeFami TF313411.

Enzyme and pathway databases

UniPathwayi UPA00190 ; UER00304 .
UPA00190 ; UER00305 .
Reactomei REACT_213534. Vitamins B6 activation to pyridoxal phosphate.

Miscellaneous databases

NextBioi 20870451.

Family and domain databases

Gene3Di 2.30.110.10. 1 hit.
HAMAPi MF_01629. PdxH.
InterProi IPR000659. Pyridox_Oxase.
IPR019740. Pyridox_Oxase_CS.
IPR011576. Pyridox_Oxase_FMN-bd.
IPR019576. Pyridoxamine_oxidase_dimer_C.
IPR012349. Split_barrel_FMN-bd.
[Graphical view ]
PANTHERi PTHR10851. PTHR10851. 1 hit.
Pfami PF10590. PNPOx_C. 1 hit.
PF01243. Pyridox_oxidase. 1 hit.
[Graphical view ]
PIRSFi PIRSF000190. Pyd_amn-ph_oxd. 1 hit.
SUPFAMi SSF50475. SSF50475. 1 hit.
TIGRFAMsi TIGR00558. pdxH. 1 hit.
PROSITEi PS01064. PYRIDOX_OXIDASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Rumen reticulum.

Entry informationi

Entry nameiPNPO_BOVIN
AccessioniPrimary (citable) accession number: Q5E9K3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: March 15, 2005
Last modified: November 26, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3