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Q5E9K3 (PNPO_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pyridoxine-5'-phosphate oxidase

EC=1.4.3.5
Alternative name(s):
Pyridoxamine-phosphate oxidase
Gene names
Name:PNPO
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length261 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the oxidation of either pyridoxine 5'-phosphate (PNP) or pyridoxamine 5'-phosphate (PMP) into pyridoxal 5'-phosphate (PLP) By similarity. HAMAP-Rule MF_01629

Catalytic activity

Pyridoxamine 5'-phosphate + H2O + O2 = pyridoxal 5'-phosphate + NH3 + H2O2. HAMAP-Rule MF_01629

Pyridoxine 5'-phosphate + O2 = pyridoxal 5'-phosphate + H2O2. HAMAP-Rule MF_01629

Cofactor

Binds 1 FMN per subunit By similarity. HAMAP-Rule MF_01629

Pathway

Cofactor biosynthesis; B6 vitamer interconversion; pyridoxal 5'-phosphate from pyridoxamine 5'-phosphate: step 1/1. HAMAP-Rule MF_01629

Cofactor biosynthesis; B6 vitamer interconversion; pyridoxal 5'-phosphate from pyridoxine 5'-phosphate: step 1/1.

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01629

Sequence similarities

Belongs to the pyridoxamine 5'-phosphate oxidase family.

Ontologies

Keywords
   Biological processPyridoxine biosynthesis
   LigandFlavoprotein
FMN
Pyridoxal phosphate
   Molecular functionOxidoreductase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processpyridoxine biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionFMN binding

Inferred from electronic annotation. Source: InterPro

pyridoxamine-phosphate oxidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 261261Pyridoxine-5'-phosphate oxidase HAMAP-Rule MF_01629
PRO_0000167782

Regions

Nucleotide binding110 – 1112FMN By similarity
Nucleotide binding174 – 1752FMN By similarity
Region225 – 2273Substrate binding By similarity

Sites

Binding site951FMN By similarity
Binding site981FMN; via amide nitrogen By similarity
Binding site1001Substrate By similarity
Binding site1171FMN By similarity
Binding site1571Substrate By similarity
Binding site1611Substrate By similarity
Binding site1651Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5E9K3 [UniParc].

Last modified March 15, 2005. Version 1.
Checksum: 7BDBB5478C6704AD

FASTA26130,366
        10         20         30         40         50         60 
MIFWLRSVIV TFGRPAEWPR CLRHLCSRGA AMDLGPMRKT YRGDPEAFEE THLTSLDPVK 

        70         80         90        100        110        120 
QFAAWFEEAV QCPDIMEANA MCLATCTRDG KPSARMVLLK GFGKDGFRFF TNFESRKGKE 

       130        140        150        160        170        180 
LDSNPFASLV FYWEPLHRQV RVEGPVKKLP EEEAECYFHS RPKSSQIGAV VSHQSSVIPD 

       190        200        210        220        230        240 
REYLRKKNKE LEQLYQEQEV PKPKYWGGYI LYPQVMEFWQ GQTNRLHDRI VFRRGLLTGD 

       250        260 
SPLGPMTHRG EEDWVYERLA P 

« Hide

References

[1]"Characterization of 954 bovine full-CDS cDNA sequences."
Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L.
BMC Genomics 6:166-166(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[2]NIH - Mammalian Gene Collection (MGC) project
Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Hereford.
Tissue: Rumen reticulum.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BT020917 mRNA. Translation: AAX08934.1.
BC103208 mRNA. Translation: AAI03209.1.
RefSeqNP_001014907.1. NM_001014907.1.
UniGeneBt.24214.

3D structure databases

ProteinModelPortalQ5E9K3.
SMRQ5E9K3. Positions 49-261.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9913.ENSBTAP00000016263.

Proteomic databases

PRIDEQ5E9K3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSBTAT00000016263; ENSBTAP00000016263; ENSBTAG00000012259.
GeneID512573.
KEGGbta:512573.

Organism-specific databases

CTD55163.

Phylogenomic databases

eggNOGCOG0259.
GeneTreeENSGT00390000011219.
HOGENOMHOG000242755.
HOVERGENHBG045634.
InParanoidQ5E9K3.
KOK00275.
OMAPEHWGGY.
OrthoDBEOG7B8S4P.
TreeFamTF313411.

Enzyme and pathway databases

UniPathwayUPA00190; UER00304.
UPA00190; UER00305.

Family and domain databases

Gene3D2.30.110.10. 1 hit.
HAMAPMF_01629. PdxH.
InterProIPR000659. Pyridox_Oxase.
IPR019740. Pyridox_Oxase_CS.
IPR011576. Pyridox_Oxase_FMN-bd.
IPR019576. Pyridoxamine_oxidase_dimer_C.
IPR012349. Split_barrel_FMN-bd.
[Graphical view]
PANTHERPTHR10851. PTHR10851. 1 hit.
PfamPF10590. PNPOx_C. 1 hit.
PF01243. Pyridox_oxidase. 1 hit.
[Graphical view]
PIRSFPIRSF000190. Pyd_amn-ph_oxd. 1 hit.
SUPFAMSSF50475. SSF50475. 1 hit.
TIGRFAMsTIGR00558. pdxH. 1 hit.
PROSITEPS01064. PYRIDOX_OXIDASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20870451.

Entry information

Entry namePNPO_BOVIN
AccessionPrimary (citable) accession number: Q5E9K3
Entry history
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: March 15, 2005
Last modified: April 16, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways