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Q5E9K3

- PNPO_BOVIN

UniProt

Q5E9K3 - PNPO_BOVIN

Protein

Pyridoxine-5'-phosphate oxidase

Gene

PNPO

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 76 (01 Oct 2014)
      Sequence version 1 (15 Mar 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the oxidation of either pyridoxine 5'-phosphate (PNP) or pyridoxamine 5'-phosphate (PMP) into pyridoxal 5'-phosphate (PLP).By similarity

    Catalytic activityi

    Pyridoxamine 5'-phosphate + H2O + O2 = pyridoxal 5'-phosphate + NH3 + H2O2.
    Pyridoxine 5'-phosphate + O2 = pyridoxal 5'-phosphate + H2O2.

    Cofactori

    Binds 1 FMN per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei95 – 951FMNBy similarity
    Binding sitei98 – 981FMN; via amide nitrogenBy similarity
    Binding sitei100 – 1001SubstrateBy similarity
    Binding sitei117 – 1171FMNBy similarity
    Binding sitei157 – 1571SubstrateBy similarity
    Binding sitei161 – 1611SubstrateBy similarity
    Binding sitei165 – 1651SubstrateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi110 – 1112FMNBy similarity
    Nucleotide bindingi174 – 1752FMNBy similarity

    GO - Molecular functioni

    1. FMN binding Source: InterPro
    2. pyridoxamine-phosphate oxidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. pyridoxine biosynthetic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Pyridoxine biosynthesis

    Keywords - Ligandi

    Flavoprotein, FMN, Pyridoxal phosphate

    Enzyme and pathway databases

    ReactomeiREACT_213534. Vitamins B6 activation to pyridoxal phosphate.
    UniPathwayiUPA00190; UER00304.
    UPA00190; UER00305.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pyridoxine-5'-phosphate oxidase (EC:1.4.3.5)
    Alternative name(s):
    Pyridoxamine-phosphate oxidase
    Gene namesi
    Name:PNPO
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 19

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 261261Pyridoxine-5'-phosphate oxidasePRO_0000167782Add
    BLAST

    Proteomic databases

    PRIDEiQ5E9K3.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    STRINGi9913.ENSBTAP00000016263.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5E9K3.
    SMRiQ5E9K3. Positions 49-261.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni225 – 2273Substrate bindingBy similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0259.
    GeneTreeiENSGT00390000011219.
    HOGENOMiHOG000242755.
    HOVERGENiHBG045634.
    InParanoidiQ5E9K3.
    KOiK00275.
    OMAiELDHRGF.
    OrthoDBiEOG7B8S4P.
    TreeFamiTF313411.

    Family and domain databases

    Gene3Di2.30.110.10. 1 hit.
    HAMAPiMF_01629. PdxH.
    InterProiIPR000659. Pyridox_Oxase.
    IPR019740. Pyridox_Oxase_CS.
    IPR011576. Pyridox_Oxase_FMN-bd.
    IPR019576. Pyridoxamine_oxidase_dimer_C.
    IPR012349. Split_barrel_FMN-bd.
    [Graphical view]
    PANTHERiPTHR10851. PTHR10851. 1 hit.
    PfamiPF10590. PNPOx_C. 1 hit.
    PF01243. Pyridox_oxidase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000190. Pyd_amn-ph_oxd. 1 hit.
    SUPFAMiSSF50475. SSF50475. 1 hit.
    TIGRFAMsiTIGR00558. pdxH. 1 hit.
    PROSITEiPS01064. PYRIDOX_OXIDASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q5E9K3-1 [UniParc]FASTAAdd to Basket

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    MIFWLRSVIV TFGRPAEWPR CLRHLCSRGA AMDLGPMRKT YRGDPEAFEE    50
    THLTSLDPVK QFAAWFEEAV QCPDIMEANA MCLATCTRDG KPSARMVLLK 100
    GFGKDGFRFF TNFESRKGKE LDSNPFASLV FYWEPLHRQV RVEGPVKKLP 150
    EEEAECYFHS RPKSSQIGAV VSHQSSVIPD REYLRKKNKE LEQLYQEQEV 200
    PKPKYWGGYI LYPQVMEFWQ GQTNRLHDRI VFRRGLLTGD SPLGPMTHRG 250
    EEDWVYERLA P 261
    Length:261
    Mass (Da):30,366
    Last modified:March 15, 2005 - v1
    Checksum:i7BDBB5478C6704AD
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BT020917 mRNA. Translation: AAX08934.1.
    BC103208 mRNA. Translation: AAI03209.1.
    RefSeqiNP_001014907.1. NM_001014907.1.
    UniGeneiBt.24214.

    Genome annotation databases

    EnsembliENSBTAT00000016263; ENSBTAP00000016263; ENSBTAG00000012259.
    GeneIDi512573.
    KEGGibta:512573.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BT020917 mRNA. Translation: AAX08934.1 .
    BC103208 mRNA. Translation: AAI03209.1 .
    RefSeqi NP_001014907.1. NM_001014907.1.
    UniGenei Bt.24214.

    3D structure databases

    ProteinModelPortali Q5E9K3.
    SMRi Q5E9K3. Positions 49-261.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9913.ENSBTAP00000016263.

    Proteomic databases

    PRIDEi Q5E9K3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000016263 ; ENSBTAP00000016263 ; ENSBTAG00000012259 .
    GeneIDi 512573.
    KEGGi bta:512573.

    Organism-specific databases

    CTDi 55163.

    Phylogenomic databases

    eggNOGi COG0259.
    GeneTreei ENSGT00390000011219.
    HOGENOMi HOG000242755.
    HOVERGENi HBG045634.
    InParanoidi Q5E9K3.
    KOi K00275.
    OMAi ELDHRGF.
    OrthoDBi EOG7B8S4P.
    TreeFami TF313411.

    Enzyme and pathway databases

    UniPathwayi UPA00190 ; UER00304 .
    UPA00190 ; UER00305 .
    Reactomei REACT_213534. Vitamins B6 activation to pyridoxal phosphate.

    Miscellaneous databases

    NextBioi 20870451.

    Family and domain databases

    Gene3Di 2.30.110.10. 1 hit.
    HAMAPi MF_01629. PdxH.
    InterProi IPR000659. Pyridox_Oxase.
    IPR019740. Pyridox_Oxase_CS.
    IPR011576. Pyridox_Oxase_FMN-bd.
    IPR019576. Pyridoxamine_oxidase_dimer_C.
    IPR012349. Split_barrel_FMN-bd.
    [Graphical view ]
    PANTHERi PTHR10851. PTHR10851. 1 hit.
    Pfami PF10590. PNPOx_C. 1 hit.
    PF01243. Pyridox_oxidase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000190. Pyd_amn-ph_oxd. 1 hit.
    SUPFAMi SSF50475. SSF50475. 1 hit.
    TIGRFAMsi TIGR00558. pdxH. 1 hit.
    PROSITEi PS01064. PYRIDOX_OXIDASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    2. NIH - Mammalian Gene Collection (MGC) project
      Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Hereford.
      Tissue: Rumen reticulum.

    Entry informationi

    Entry nameiPNPO_BOVIN
    AccessioniPrimary (citable) accession number: Q5E9K3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 10, 2006
    Last sequence update: March 15, 2005
    Last modified: October 1, 2014
    This is version 76 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3