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Protein

Proteasome subunit beta type-2

Gene

PSMB2

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit has a chymotrypsin-like activity (By similarity).By similarity

Catalytic activityi

Cleavage of peptide bonds with very broad specificity.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Threonine protease

Enzyme and pathway databases

ReactomeiR-BTA-1169091. Activation of NF-kappaB in B cells.
R-BTA-1234176. Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha.
R-BTA-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-BTA-174084. Autodegradation of Cdh1 by Cdh1:APC/C.
R-BTA-174113. SCF-beta-TrCP mediated degradation of Emi1.
R-BTA-174154. APC/C:Cdc20 mediated degradation of Securin.
R-BTA-174178. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
R-BTA-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-BTA-187577. SCF(Skp2)-mediated degradation of p27/p21.
R-BTA-195253. Degradation of beta-catenin by the destruction complex.
R-BTA-202424. Downstream TCR signaling.
R-BTA-2467813. Separation of Sister Chromatids.
R-BTA-2871837. FCERI mediated NF-kB activation.
R-BTA-349425. Autodegradation of the E3 ubiquitin ligase COP1.
R-BTA-350562. Regulation of ornithine decarboxylase (ODC).
R-BTA-382556. ABC-family proteins mediated transport.
R-BTA-450408. AUF1 (hnRNP D0) binds and destabilizes mRNA.
R-BTA-4608870. Asymmetric localization of PCP proteins.
R-BTA-4641257. Degradation of AXIN.
R-BTA-4641258. Degradation of DVL.
R-BTA-5358346. Hedgehog ligand biogenesis.
R-BTA-5607761. Dectin-1 mediated noncanonical NF-kB signaling.
R-BTA-5607764. CLEC7A (Dectin-1) signaling.
R-BTA-5610780. Degradation of GLI1 by the proteasome.
R-BTA-5610785. GLI3 is processed to GLI3R by the proteasome.
R-BTA-5632684. Hedgehog 'on' state.
R-BTA-5658442. Regulation of RAS by GAPs.
R-BTA-5668541. TNFR2 non-canonical NF-kB pathway.
R-BTA-5676590. NIK-->noncanonical NF-kB signaling.
R-BTA-5687128. MAPK6/MAPK4 signaling.
R-BTA-5689603. UCH proteinases.
R-BTA-5689880. Ub-specific processing proteases.
R-BTA-68827. CDT1 association with the CDC6:ORC:origin complex.
R-BTA-68949. Orc1 removal from chromatin.
R-BTA-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-BTA-69229. Ubiquitin-dependent degradation of Cyclin D1.
R-BTA-69481. G2/M Checkpoints.
R-BTA-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
R-BTA-8852276. The role of GTSE1 in G2/M progression after G2 checkpoint.
R-BTA-8854050. FBXL7 down-regulates AURKA during mitotic entry and in early mitosis.
R-BTA-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Protein family/group databases

MEROPSiT01.984.

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome subunit beta type-2 (EC:3.4.25.1)
Gene namesi
Name:PSMB2
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 3

Subcellular locationi

  • Cytoplasm PROSITE-ProRule annotation
  • Nucleus By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002398521 – 201Proteasome subunit beta type-2Add BLAST201

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ5E9K0.
PeptideAtlasiQ5E9K0.
PRIDEiQ5E9K0.

Expressioni

Gene expression databases

BgeeiENSBTAG00000002377.

Interactioni

Subunit structurei

The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.

Protein-protein interaction databases

IntActiQ5E9K0. 1 interactor.
STRINGi9913.ENSBTAP00000003072.

Structurei

Secondary structure

1201
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 8Combined sources5
Beta strandi10 – 18Combined sources9
Beta strandi21 – 23Combined sources3
Beta strandi26 – 31Combined sources6
Beta strandi35 – 37Combined sources3
Beta strandi39 – 49Combined sources11
Helixi52 – 71Combined sources20
Helixi77 – 92Combined sources16
Beta strandi94 – 96Combined sources3
Beta strandi100 – 108Combined sources9
Turni109 – 111Combined sources3
Beta strandi112 – 118Combined sources7
Beta strandi124 – 126Combined sources3
Beta strandi128 – 133Combined sources6
Helixi134 – 146Combined sources13
Helixi153 – 169Combined sources17
Beta strandi178 – 184Combined sources7
Beta strandi187 – 189Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1IRUX-ray2.75K/Y1-201[»]
ProteinModelPortaliQ5E9K0.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ5E9K0.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase T1B family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0177. Eukaryota.
COG0638. LUCA.
GeneTreeiENSGT00640000091536.
HOGENOMiHOG000188743.
HOVERGENiHBG000815.
InParanoidiQ5E9K0.
KOiK02734.
OMAiDRYHDPE.
OrthoDBiEOG091G0K9M.
TreeFamiTF106219.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
InterProiIPR029055. Ntn_hydrolases_N.
IPR016050. Proteasome_bsu_CS.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
[Graphical view]
PfamiPF00227. Proteasome. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiPS00854. PROTEASOME_BETA_1. 1 hit.
PS51476. PROTEASOME_BETA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5E9K0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEYLIGIQGP DYVLVASDRV AASNIVQMKD DHDKMFKMSE KILLLCVGEA
60 70 80 90 100
GDTVQFAEYI QKNVQLYKMR NGYELSPTAA ANFTRRNLAD YLRSRTPYHV
110 120 130 140 150
NLLLAGYDEH EGPALYYMDY LAALAKAPFA AHGYGAFLTL SILDRYYTPT
160 170 180 190 200
ISREKAVELL RKCLEELQKR FILNLPTFSV RIIDRNGIHD LDNISFPKQG

S
Length:201
Mass (Da):22,896
Last modified:March 15, 2005 - v1
Checksum:i8D195EBA1DBB02DE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT020855 mRNA. Translation: AAX08872.1.
BT020920 mRNA. Translation: AAX08937.1.
BC102367 mRNA. Translation: AAI02368.1.
RefSeqiNP_001015615.1. NM_001015615.1.
UniGeneiBt.40863.

Genome annotation databases

EnsembliENSBTAT00000003072; ENSBTAP00000003072; ENSBTAG00000002377.
GeneIDi516919.
KEGGibta:516919.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT020855 mRNA. Translation: AAX08872.1.
BT020920 mRNA. Translation: AAX08937.1.
BC102367 mRNA. Translation: AAI02368.1.
RefSeqiNP_001015615.1. NM_001015615.1.
UniGeneiBt.40863.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1IRUX-ray2.75K/Y1-201[»]
ProteinModelPortaliQ5E9K0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ5E9K0. 1 interactor.
STRINGi9913.ENSBTAP00000003072.

Protein family/group databases

MEROPSiT01.984.

Proteomic databases

PaxDbiQ5E9K0.
PeptideAtlasiQ5E9K0.
PRIDEiQ5E9K0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000003072; ENSBTAP00000003072; ENSBTAG00000002377.
GeneIDi516919.
KEGGibta:516919.

Organism-specific databases

CTDi5690.

Phylogenomic databases

eggNOGiKOG0177. Eukaryota.
COG0638. LUCA.
GeneTreeiENSGT00640000091536.
HOGENOMiHOG000188743.
HOVERGENiHBG000815.
InParanoidiQ5E9K0.
KOiK02734.
OMAiDRYHDPE.
OrthoDBiEOG091G0K9M.
TreeFamiTF106219.

Enzyme and pathway databases

ReactomeiR-BTA-1169091. Activation of NF-kappaB in B cells.
R-BTA-1234176. Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha.
R-BTA-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-BTA-174084. Autodegradation of Cdh1 by Cdh1:APC/C.
R-BTA-174113. SCF-beta-TrCP mediated degradation of Emi1.
R-BTA-174154. APC/C:Cdc20 mediated degradation of Securin.
R-BTA-174178. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
R-BTA-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-BTA-187577. SCF(Skp2)-mediated degradation of p27/p21.
R-BTA-195253. Degradation of beta-catenin by the destruction complex.
R-BTA-202424. Downstream TCR signaling.
R-BTA-2467813. Separation of Sister Chromatids.
R-BTA-2871837. FCERI mediated NF-kB activation.
R-BTA-349425. Autodegradation of the E3 ubiquitin ligase COP1.
R-BTA-350562. Regulation of ornithine decarboxylase (ODC).
R-BTA-382556. ABC-family proteins mediated transport.
R-BTA-450408. AUF1 (hnRNP D0) binds and destabilizes mRNA.
R-BTA-4608870. Asymmetric localization of PCP proteins.
R-BTA-4641257. Degradation of AXIN.
R-BTA-4641258. Degradation of DVL.
R-BTA-5358346. Hedgehog ligand biogenesis.
R-BTA-5607761. Dectin-1 mediated noncanonical NF-kB signaling.
R-BTA-5607764. CLEC7A (Dectin-1) signaling.
R-BTA-5610780. Degradation of GLI1 by the proteasome.
R-BTA-5610785. GLI3 is processed to GLI3R by the proteasome.
R-BTA-5632684. Hedgehog 'on' state.
R-BTA-5658442. Regulation of RAS by GAPs.
R-BTA-5668541. TNFR2 non-canonical NF-kB pathway.
R-BTA-5676590. NIK-->noncanonical NF-kB signaling.
R-BTA-5687128. MAPK6/MAPK4 signaling.
R-BTA-5689603. UCH proteinases.
R-BTA-5689880. Ub-specific processing proteases.
R-BTA-68827. CDT1 association with the CDC6:ORC:origin complex.
R-BTA-68949. Orc1 removal from chromatin.
R-BTA-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-BTA-69229. Ubiquitin-dependent degradation of Cyclin D1.
R-BTA-69481. G2/M Checkpoints.
R-BTA-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
R-BTA-8852276. The role of GTSE1 in G2/M progression after G2 checkpoint.
R-BTA-8854050. FBXL7 down-regulates AURKA during mitotic entry and in early mitosis.
R-BTA-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

EvolutionaryTraceiQ5E9K0.

Gene expression databases

BgeeiENSBTAG00000002377.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
InterProiIPR029055. Ntn_hydrolases_N.
IPR016050. Proteasome_bsu_CS.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
[Graphical view]
PfamiPF00227. Proteasome. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiPS00854. PROTEASOME_BETA_1. 1 hit.
PS51476. PROTEASOME_BETA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPSB2_BOVIN
AccessioniPrimary (citable) accession number: Q5E9K0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: March 15, 2005
Last modified: November 30, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.