Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Synembryn-A

Gene

RIC8A

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Guanine nucleotide exchange factor (GEF), which can activate some, but not all, G-alpha proteins. Able to activate GNAI1, GNAO1 and GNAQ, but not GNAS by exchanging bound GDP for free GTP. Involved in regulation of microtubule pulling forces during mitotic movement of chromosomes by stimulating G(i)-alpha protein, possibly leading to release G(i)-alpha-GTP and NuMA proteins from the NuMA-GPSM2-G(i)-alpha-GDP complex. Also acts as an activator for G(q)-alpha (GNAQ) protein by enhancing the G(q)-coupled receptor-mediated ERK activation (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Names & Taxonomyi

Protein namesi
Recommended name:
Synembryn-A
Alternative name(s):
Protein Ric-8A
Gene namesi
Name:RIC8A
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 11

Subcellular locationi

  • Cytoplasm By similarity
  • Cell membrane By similarity

  • Note: Colocalizes with RIC8A in CA2 hippocampal neurons. Colocalizes with GNAI1 and RGS14 at the plasma membrane (By similarity).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 530530Synembryn-APRO_0000235889Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei435 – 4351PhosphoserineBy similarity
Modified residuei440 – 4401PhosphothreonineBy similarity
Modified residuei442 – 4421PhosphothreonineBy similarity
Modified residuei501 – 5011PhosphoserineBy similarity
Modified residuei522 – 5221PhosphoserineBy similarity
Modified residuei523 – 5231PhosphoserineBy similarity
Modified residuei527 – 5271PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ5E9J8.
PRIDEiQ5E9J8.

Interactioni

Subunit structurei

Interacts with GDP-bound G alpha proteins GNAI1, GNAO1 and GNAQ, and with GNA13 with lower affinity. Does not interact with G-alpha proteins when they are in complex with subunits beta and gamma. Interacts (via C-terminus) with RGS14; the interaction stimulates the dissociation of the complex between RGS14 and the active GTP-bound form of GNAI1 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000002646.

Family & Domainsi

Sequence similaritiesi

Belongs to the synembryn family.Curated

Phylogenomic databases

eggNOGiKOG4464. Eukaryota.
ENOG410XSCJ. LUCA.
GeneTreeiENSGT00390000014700.
HOGENOMiHOG000236345.
HOVERGENiHBG054867.
InParanoidiQ5E9J8.
OMAiPMGMSPR.
OrthoDBiEOG72JWG4.
TreeFamiTF314907.

Family and domain databases

Gene3Di1.25.10.10. 3 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR019318. Gua_nucleotide_exch_fac_Ric8.
IPR008376. Synembryn.
[Graphical view]
PfamiPF10165. Ric8. 1 hit.
[Graphical view]
PRINTSiPR01802. SYNEMBRYN.
SUPFAMiSSF48371. SSF48371. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5E9J8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEPRAVADAL ETGEEDVVME ALRAYNRENS QSFTFDDAQQ EDRKRLAKLL
60 70 80 90 100
VSVLEQGLPP SRRVIWLQSI RILSRDRSCL DSFTSRRSLQ ALACYAGISA
110 120 130 140 150
SQGSVPEPLN MDVVLESLKC LCNLVLSSPV AQALAAEAGL VVRLAERVGL
160 170 180 190 200
CRQSSFPHDV QFFDLRLLFL LTALRTDVRQ QLFQELQGVR LLTRALELTL
210 220 230 240 250
GMTEGERHPE LLPPQETERA MEILKVLFNI TFDSIKREVD EEDAALYRHL
260 270 280 290 300
GTLLRHCVML AAAGDRTEEL HGHAVNLLGN LPVKCLDVLL TLEPHEGSLE
310 320 330 340 350
FLGVNMDVIR VLLSFMEKRL HQTHRLKESV APVLSVLTEC ARMHRPARKF
360 370 380 390 400
LKAQVLPPLR DVRTRPEVGE LLRNKLVRLM THLDTDVKRV AAEFLFVLCS
410 420 430 440 450
ESVPRFIKYT GYGNAAGLLA ARGLMAGGRP EGQYSEDEDT DTDEYKEAKA
460 470 480 490 500
SINPVTGRVE EKPPNPMEGM TEEQKEHEAM KLVNMFDKLS RHRVIQPMGM
510 520 530
SPRGQLTSLQ DAMCETMEGQ LSSDPDSDPD
Length:530
Mass (Da):59,499
Last modified:March 15, 2005 - v1
Checksum:iF663746DB9F588EB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT020922 mRNA. Translation: AAX08939.1.
BC118313 mRNA. Translation: AAI18314.1.
RefSeqiNP_001015627.1. NM_001015627.2.
UniGeneiBt.3803.

Genome annotation databases

EnsembliENSBTAT00000002646; ENSBTAP00000002646; ENSBTAG00000002042.
GeneIDi521037.
KEGGibta:521037.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT020922 mRNA. Translation: AAX08939.1.
BC118313 mRNA. Translation: AAI18314.1.
RefSeqiNP_001015627.1. NM_001015627.2.
UniGeneiBt.3803.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000002646.

Proteomic databases

PaxDbiQ5E9J8.
PRIDEiQ5E9J8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000002646; ENSBTAP00000002646; ENSBTAG00000002042.
GeneIDi521037.
KEGGibta:521037.

Organism-specific databases

CTDi60626.

Phylogenomic databases

eggNOGiKOG4464. Eukaryota.
ENOG410XSCJ. LUCA.
GeneTreeiENSGT00390000014700.
HOGENOMiHOG000236345.
HOVERGENiHBG054867.
InParanoidiQ5E9J8.
OMAiPMGMSPR.
OrthoDBiEOG72JWG4.
TreeFamiTF314907.

Family and domain databases

Gene3Di1.25.10.10. 3 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR019318. Gua_nucleotide_exch_fac_Ric8.
IPR008376. Synembryn.
[Graphical view]
PfamiPF10165. Ric8. 1 hit.
[Graphical view]
PRINTSiPR01802. SYNEMBRYN.
SUPFAMiSSF48371. SSF48371. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Fetal skin.

Entry informationi

Entry nameiRIC8A_BOVIN
AccessioniPrimary (citable) accession number: Q5E9J8
Secondary accession number(s): Q17QK2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 16, 2006
Last sequence update: March 15, 2005
Last modified: June 8, 2016
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.