Reviewed, UniProtKB/Swiss-Prot Q5E9J5 (DHCR7_BOVIN)

Names and origin

Protein names

Recommended name:
    7-dehydrocholesterol reductase
      Short name=7-DHC reductase
    EC=1.3.1.21
Alternative name(s):
    Sterol Delta(7)-reductase

Gene names

Name:

DHCR7

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	475 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Production of cholesterol by reduction of C7-C8 double bond of 7-dehydrocholesterol (7-DHC) By similarity.
Catalytic activity	Cholesterol + NADP+ = cholesta-5,7-dien-3-beta-ol + NADPH.
Pathway	Steroid biosynthesis; cholesterol biosynthesis.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the ERG4/ERG24 family.

Ontologies

Keywords
Biological process	Cholesterol biosynthesis Lipid synthesis Steroid biosynthesis Sterol biosynthesis
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Ligand	NADP
Molecular function	Oxidoreductase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	cholesterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	7-dehydrocholesterol reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 475	475	7-dehydrocholesterol reductase		PRO_0000207501
Regions
	Transmembrane	40 – 60	21	Potential
	Transmembrane	151 – 173	23	Potential
	Transmembrane	178 – 200	23	Potential
	Transmembrane	266 – 286	21	Potential
	Transmembrane	306 – 326	21	Potential
	Transmembrane	331 – 351	21	Potential
	Transmembrane	420 – 440	21	Potential
Amino acid modifications
	Modified residue	14	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5E9J5-1 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: 4712E8DBA76D0A91	FASTA	475	54,271
	10 20 30 40 50 60 MAAKSQPSAP KTKSTSGLTN GNAAAQGQWG RAWEVDWFSL ASVIFLLLFA PFIVYYFIMA 70 80 90 100 110 120 CDQYGCSLTV PVADLATGRA RLADIWARTP PVTAKAAQIY TAWVTLQVLL YMLLPDFCHK 130 140 150 160 170 180 FLPGYVGGVQ EGAVTPAGAV NKYEINGLQA WLLTHLLWFA NAHLLGWFSP TIIFDNWIPL 190 200 210 220 230 240 LWCANILGYT VSTFAMVKGY LFPTDARECK FTGNFFYNYM MGVEFNPRIG KWFDFKLFFN 250 260 270 280 290 300 GRPGIVAWTL INLSFAAKQQ ELYGHVTNSM VLVNILQAIY VLDFFWNETW YLKTIDICHD 310 320 330 340 350 360 HFGWYLGWGD CVWLPYLYTL QGLYLVYHPV QLPTYYALGV LLLGLLGYYI FRMTNHQKDL 370 380 390 400 410 420 FRRTDGRCLI WGRKPKAIEC SYTSADGQRH HSKLLVSGFW GVARHFNYTG DLMGSLAYCL 430 440 450 460 470 ACGGGHLLPY FYIIFMAILL THRCLRDEHR CANKYGRDWE HYTAAVPYRL LPGIF

References

[1]	"Characterization of 954 bovine full-CDS cDNA sequences." Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L. BMC Genomics 6:166-166(2005) [PubMed: 16305752] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[2]	NIH - Mammalian Gene Collection (MGC) project Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Fetal skin.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BT020925 mRNA. Translation: AAX08942.1. BC150084 mRNA. Translation: AAI50085.1.
IPI	IPI00697452.
RefSeq	NP_001014927.1.
UniGene	Bt.5449
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q5E9J5.
Genome annotation databases
Ensembl	ENSBTAT00000021892; ENSBTAP00000021892; ENSBTAG00000016465; Bos taurus. [Genome view]
GeneID	514745.
KEGG	bta:514745.
Organism-specific databases
CTD	514745.
Phylogenomic databases
eggNOG	maNOG10004.
HOVERGEN	Q5E9J5.
InParanoid	Q5E9J5.
OMA	KDCKFTG.
OrthoDB	EOG94QWM8.
PhylomeDB	Q5E9J5.
Enzyme and pathway databases
BRENDA	1.3.1.21. 251.
Family and domain databases
InterPro	IPR001171. Ergosterol_biosynth_ERG4_ERG24. IPR018083. Sterol_reductase_CS. [Graphical view]
Pfam	PF01222. ERG4_ERG24. 1 hit. [Graphical view]
PROSITE	PS01017. STEROL_REDUCT_1. 1 hit. PS01018. STEROL_REDUCT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHCR7_BOVIN

Accession

Primary (citable) accession number: Q5E9J5
Secondary accession number(s): A6QR24

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2005
Last sequence update:	March 15, 2005
Last modified:	January 19, 2010
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)