Q5E9G0 (GSTA4_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutathione S-transferase A4
EC=2.5.1.18

Alternative name(s):
GST class-alpha member 4
Glutathione S-transferase alpha-4

Gene names

Name:

GSTA4

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	222 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles By similarity.
Catalytic activity	RX + glutathione = HX + R-S-glutathione.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the GST superfamily. Alpha family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	glutathione metabolic process Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glutathione transferase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 222	222	Glutathione S-transferase A4		PRO_0000249771
Regions
	Domain	3 – 83	81	GST N-terminal
	Domain	85 – 208	124	GST C-terminal
	Region	54 – 55	2	Glutathione binding By similarity
	Region	67 – 68	2	Glutathione binding By similarity
Sites
	Binding site	9	1	Glutathione By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5E9G0 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: A0C06C858741A67B	FASTA	222	25,659
	10 20 30 40 50 60 MATKPKLHYP NGRGRMESVR WVLAAAGVEF DEEFLETKEQ LQKLQDGNHL LFQQVPMVEI 70 80 90 100 110 120 DGMKLVQTRS ILHYIADKHH LFGKDLKERT LIDMYVEGTL DLLELLIMHP FLKPDDQQKE 130 140 150 160 170 180 VANMAQKAII RYFPVFEKVL RGHGQRFLVG NQLSLADIIL LQTILALEEK IPNILSAFPH 190 200 210 220 LQEYTVKISN IPTIKKFLEP GSKKKPPPDD IYVRTVYNIF MP

References

[1]	"Characterization of 954 bovine full-CDS cDNA sequences." Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L. BMC Genomics 6:166-166(2005) [PubMed: 16305752] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	BT020960 mRNA. Translation: AAX08977.1.
IPI	IPI00704883.
RefSeq	NP_001015651.1. NM_001015651.1.
UniGene	Bt.55005.
3D structure databases
HSSP	HSSP built from PDB template 1GUL based on UniProtKB O15217.
ProteinModelPortal	Q5E9G0.
SMR	Q5E9G0. Positions 4-220.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5E9G0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000028640; ENSBTAP00000028640; ENSBTAG00000004288.
GeneID	533917.
KEGG	bta:533917.
Organism-specific databases
CTD	2941.
Phylogenomic databases
GeneTree	ENSGT00550000074445.
HOVERGEN	HBG053749.
InParanoid	Q5E9G0.
OMA	TVYNIFM.
OrthoDB	EOG4N5VXR.
Family and domain databases
InterPro	IPR010987. Glutathione-S-Trfase_C-like. IPR004045. Glutathione_S-Trfase_N. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR003080. GST_alpha. IPR004046. GST_C. IPR012336. Thioredoxin-like_fold. [Graphical view]
Gene3D	G3DSA:1.20.1050.10. GST_C_like. 1 hit. G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
KO	K00799.
PANTHER	PTHR11571:SF4. GST_alpha. 1 hit.
Pfam	PF00043. GST_C. 1 hit. PF02798. GST_N. 1 hit. [Graphical view]
PRINTS	PR01266. GSTRNSFRASEA.
SUPFAM	SSF47616. GST_C_like. 1 hit. SSF52833. Thiordxn-like_fd. 1 hit.
PROSITE	PS50405. GST_CTER. 1 hit. PS50404. GST_NTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSTA4_BOVIN

Accession

Primary (citable) accession number: Q5E9G0

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2006
Last sequence update:	March 15, 2005
Last modified:	November 16, 2011
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program