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Q5E9F7

- COF1_BOVIN

UniProt

Q5E9F7 - COF1_BOVIN

Protein

Cofilin-1

Gene

CFL1

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
  1. Functioni

    Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity. Regulates actin cytoskeleton dynamics. Important for normal progress through mitosis and normal cytokinesis. Plays a role in the regulation of cell morphology and cytoskeletal organization By similarity. Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation By similarity.By similarity

    GO - Biological processi

    1. actin filament depolymerization Source: InterPro
    2. cytoskeleton organization Source: UniProtKB
    3. establishment of cell polarity Source: Ensembl
    4. neural crest cell migration Source: Ensembl
    5. neural fold formation Source: Ensembl
    6. positive regulation of actin filament depolymerization Source: Ensembl
    7. protein phosphorylation Source: Ensembl
    8. regulation of cell morphogenesis Source: UniProtKB
    9. response to amino acid Source: Ensembl
    10. response to virus Source: Ensembl

    Keywords - Ligandi

    Actin-binding

    Enzyme and pathway databases

    ReactomeiREACT_205196. Sema3A PAK dependent Axon repulsion.
    REACT_211475. Regulation of actin dynamics for phagocytic cup formation.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cofilin-1
    Alternative name(s):
    Cofilin, non-muscle isoform
    Gene namesi
    Name:CFL1
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 29

    Subcellular locationi

    Nucleus matrix By similarity. Cytoplasmcytoskeleton By similarity. Cell projectionruffle membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity. Cell projectionlamellipodium membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
    Note: Colocalizes with the actin cytoskeleton in membrane ruffles and lamellipodia. Detected at the cleavage furrow and contractile ring during cytokinesis. Almost completely in nucleus in cells exposed to heat shock By similarity.By similarity

    GO - Cellular componenti

    1. cortical actin cytoskeleton Source: Ensembl
    2. lamellipodium membrane Source: UniProtKB-SubCell
    3. nuclear matrix Source: UniProtKB-SubCell
    4. ruffle membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 166165Cofilin-1PRO_0000214897Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineBy similarity
    Modified residuei3 – 31PhosphoserineBy similarity
    Modified residuei8 – 81PhosphoserineBy similarity
    Modified residuei13 – 131N6-acetyllysineBy similarity
    Modified residuei25 – 251PhosphothreonineBy similarity
    Modified residuei68 – 681PhosphotyrosineBy similarity
    Modified residuei73 – 731N6-acetyllysineBy similarity
    Modified residuei140 – 1401PhosphotyrosineBy similarity
    Modified residuei144 – 1441N6-acetyllysineBy similarity
    Modified residuei156 – 1561PhosphoserineBy similarity

    Post-translational modificationi

    Inactivated by phosphorylation on Ser-3. Phosphorylated on Ser-3 in resting cells By similarity. Dephosphorylated by PDXP/chronophin; this restores its activity in promoting actin filament depolymerization. The phosphorylation of Ser-24 may prevent recognition of the nuclear localization signal By similarity. Phosphorylated via a ARRB1-RAC1-LIMK1-PAK1 cascade upon active ligand stimulation of atypical chemokine receptor ACKR2 By similarity.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PaxDbiQ5E9F7.
    PRIDEiQ5E9F7.

    Interactioni

    Subunit structurei

    Can bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is a major component of intranuclear and cytoplasmic actin rods By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ5E9F7.
    SMRiQ5E9F7. Positions 1-166.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini4 – 153150ADF-HPROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi30 – 345Nuclear localization signalSequence Analysis

    Sequence similaritiesi

    Belongs to the actin-binding proteins ADF family.Curated
    Contains 1 ADF-H domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG286948.
    GeneTreeiENSGT00440000033289.
    HOVERGENiHBG000381.
    KOiK05765.
    OMAiSKQIHTM.
    OrthoDBiEOG7353Z9.
    TreeFamiTF328601.

    Family and domain databases

    Gene3Di3.40.20.10. 1 hit.
    InterProiIPR002108. ADF-H.
    IPR029006. ADF-H/Gelsolin-like_dom.
    IPR017904. ADF/Cofilin/Destrin.
    IPR027234. Cofilin_1.
    [Graphical view]
    PANTHERiPTHR11913. PTHR11913. 1 hit.
    PTHR11913:SF17. PTHR11913:SF17. 1 hit.
    PfamiPF00241. Cofilin_ADF. 1 hit.
    [Graphical view]
    PRINTSiPR00006. COFILIN.
    SMARTiSM00102. ADF. 1 hit.
    [Graphical view]
    PROSITEiPS51263. ADF_H. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q5E9F7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASGVAVSDG VIKVFNDMKV RKSSTPEEVK KRKKAVLFCL SEDKKNIILE    50
    EGKEILVGDV GQTVDDPYAT FVKMLPDKDC RYALYDATYE TKESKKEDLV 100
    FIFWAPECAP LKSKMIYASS KDAIKKKLTG IKHELQANCY EEVKDRCTLA 150
    EKLGGSAVIS LEGKPL 166
    Length:166
    Mass (Da):18,519
    Last modified:January 23, 2007 - v3
    Checksum:i589EE8EC1ED12719
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BT020963 mRNA. Translation: AAX08980.1.
    BC103077 mRNA. Translation: AAI03078.1.
    RefSeqiNP_001015655.1. NM_001015655.1.
    UniGeneiBt.52428.

    Genome annotation databases

    EnsembliENSBTAT00000028602; ENSBTAP00000028602; ENSBTAG00000021455.
    GeneIDi534553.
    KEGGibta:534553.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BT020963 mRNA. Translation: AAX08980.1 .
    BC103077 mRNA. Translation: AAI03078.1 .
    RefSeqi NP_001015655.1. NM_001015655.1.
    UniGenei Bt.52428.

    3D structure databases

    ProteinModelPortali Q5E9F7.
    SMRi Q5E9F7. Positions 1-166.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PaxDbi Q5E9F7.
    PRIDEi Q5E9F7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000028602 ; ENSBTAP00000028602 ; ENSBTAG00000021455 .
    GeneIDi 534553.
    KEGGi bta:534553.

    Organism-specific databases

    CTDi 1072.

    Phylogenomic databases

    eggNOGi NOG286948.
    GeneTreei ENSGT00440000033289.
    HOVERGENi HBG000381.
    KOi K05765.
    OMAi SKQIHTM.
    OrthoDBi EOG7353Z9.
    TreeFami TF328601.

    Enzyme and pathway databases

    Reactomei REACT_205196. Sema3A PAK dependent Axon repulsion.
    REACT_211475. Regulation of actin dynamics for phagocytic cup formation.

    Miscellaneous databases

    NextBioi 20876442.

    Family and domain databases

    Gene3Di 3.40.20.10. 1 hit.
    InterProi IPR002108. ADF-H.
    IPR029006. ADF-H/Gelsolin-like_dom.
    IPR017904. ADF/Cofilin/Destrin.
    IPR027234. Cofilin_1.
    [Graphical view ]
    PANTHERi PTHR11913. PTHR11913. 1 hit.
    PTHR11913:SF17. PTHR11913:SF17. 1 hit.
    Pfami PF00241. Cofilin_ADF. 1 hit.
    [Graphical view ]
    PRINTSi PR00006. COFILIN.
    SMARTi SM00102. ADF. 1 hit.
    [Graphical view ]
    PROSITEi PS51263. ADF_H. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    2. NIH - Mammalian Gene Collection (MGC) project
      Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Crossbred X Angus.
      Tissue: Ileum.

    Entry informationi

    Entry nameiCOF1_BOVIN
    AccessioniPrimary (citable) accession number: Q5E9F7
    Secondary accession number(s): Q3SZ74
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 13, 2005
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 73 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3