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Protein

Cofilin-1

Gene

CFL1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity. Regulates actin cytoskeleton dynamics. Important for normal progress through mitosis and normal cytokinesis. Plays a role in the regulation of cell morphology and cytoskeletal organization (By similarity). Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation (By similarity).By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

Actin-binding

Enzyme and pathway databases

ReactomeiREACT_272065. Platelet degranulation.
REACT_307278. EPHB-mediated forward signaling.
REACT_319858. Regulation of actin dynamics for phagocytic cup formation.
REACT_349437. Sema3A PAK dependent Axon repulsion.

Names & Taxonomyi

Protein namesi
Recommended name:
Cofilin-1
Alternative name(s):
Cofilin, non-muscle isoform
Gene namesi
Name:CFL1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136 Componenti: Chromosome 29

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 166165Cofilin-1PRO_0000214897Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei3 – 31PhosphoserineBy similarity
Modified residuei8 – 81PhosphoserineBy similarity
Modified residuei13 – 131N6-acetyllysineBy similarity
Modified residuei25 – 251PhosphothreonineBy similarity
Modified residuei68 – 681PhosphotyrosineBy similarity
Modified residuei73 – 731N6-acetyllysineBy similarity
Modified residuei140 – 1401PhosphotyrosineBy similarity
Modified residuei144 – 1441N6-acetyllysineBy similarity
Modified residuei156 – 1561PhosphoserineBy similarity

Post-translational modificationi

Inactivated by phosphorylation on Ser-3. Phosphorylated on Ser-3 in resting cells (By similarity). Dephosphorylated by PDXP/chronophin; this restores its activity in promoting actin filament depolymerization. The phosphorylation of Ser-24 may prevent recognition of the nuclear localization signal (By similarity). Phosphorylated via a ARRB1-RAC1-LIMK1-PAK1 cascade upon active ligand stimulation of atypical chemokine receptor ACKR2 (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ5E9F7.
PRIDEiQ5E9F7.

Expressioni

Gene expression databases

ExpressionAtlasiQ5E9F7. baseline.

Interactioni

Subunit structurei

Can bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is a major component of intranuclear and cytoplasmic actin rods (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliQ5E9F7.
SMRiQ5E9F7. Positions 1-166.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 153150ADF-HPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi30 – 345Nuclear localization signalSequence Analysis

Sequence similaritiesi

Belongs to the actin-binding proteins ADF family.Curated
Contains 1 ADF-H domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG286948.
GeneTreeiENSGT00440000033289.
HOVERGENiHBG000381.
InParanoidiQ5E9F7.
KOiK05765.
OMAiPPSCQTG.
OrthoDBiEOG7353Z9.
TreeFamiTF328601.

Family and domain databases

Gene3Di3.40.20.10. 1 hit.
InterProiIPR002108. ADF-H.
IPR029006. ADF-H/Gelsolin-like_dom.
IPR017904. ADF/Cofilin/Destrin.
IPR027234. Cofilin_1.
[Graphical view]
PANTHERiPTHR11913. PTHR11913. 1 hit.
PTHR11913:SF17. PTHR11913:SF17. 1 hit.
PfamiPF00241. Cofilin_ADF. 1 hit.
[Graphical view]
PRINTSiPR00006. COFILIN.
SMARTiSM00102. ADF. 1 hit.
[Graphical view]
PROSITEiPS51263. ADF_H. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5E9F7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASGVAVSDG VIKVFNDMKV RKSSTPEEVK KRKKAVLFCL SEDKKNIILE
60 70 80 90 100
EGKEILVGDV GQTVDDPYAT FVKMLPDKDC RYALYDATYE TKESKKEDLV
110 120 130 140 150
FIFWAPECAP LKSKMIYASS KDAIKKKLTG IKHELQANCY EEVKDRCTLA
160
EKLGGSAVIS LEGKPL
Length:166
Mass (Da):18,519
Last modified:January 23, 2007 - v3
Checksum:i589EE8EC1ED12719
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT020963 mRNA. Translation: AAX08980.1.
BC103077 mRNA. Translation: AAI03078.1.
RefSeqiNP_001015655.1. NM_001015655.1.
UniGeneiBt.52428.

Genome annotation databases

EnsembliENSBTAT00000028602; ENSBTAP00000028602; ENSBTAG00000021455.
GeneIDi534553.
KEGGibta:534553.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT020963 mRNA. Translation: AAX08980.1.
BC103077 mRNA. Translation: AAI03078.1.
RefSeqiNP_001015655.1. NM_001015655.1.
UniGeneiBt.52428.

3D structure databases

ProteinModelPortaliQ5E9F7.
SMRiQ5E9F7. Positions 1-166.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PaxDbiQ5E9F7.
PRIDEiQ5E9F7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000028602; ENSBTAP00000028602; ENSBTAG00000021455.
GeneIDi534553.
KEGGibta:534553.

Organism-specific databases

CTDi1072.

Phylogenomic databases

eggNOGiNOG286948.
GeneTreeiENSGT00440000033289.
HOVERGENiHBG000381.
InParanoidiQ5E9F7.
KOiK05765.
OMAiPPSCQTG.
OrthoDBiEOG7353Z9.
TreeFamiTF328601.

Enzyme and pathway databases

ReactomeiREACT_272065. Platelet degranulation.
REACT_307278. EPHB-mediated forward signaling.
REACT_319858. Regulation of actin dynamics for phagocytic cup formation.
REACT_349437. Sema3A PAK dependent Axon repulsion.

Miscellaneous databases

NextBioi20876442.

Gene expression databases

ExpressionAtlasiQ5E9F7. baseline.

Family and domain databases

Gene3Di3.40.20.10. 1 hit.
InterProiIPR002108. ADF-H.
IPR029006. ADF-H/Gelsolin-like_dom.
IPR017904. ADF/Cofilin/Destrin.
IPR027234. Cofilin_1.
[Graphical view]
PANTHERiPTHR11913. PTHR11913. 1 hit.
PTHR11913:SF17. PTHR11913:SF17. 1 hit.
PfamiPF00241. Cofilin_ADF. 1 hit.
[Graphical view]
PRINTSiPR00006. COFILIN.
SMARTiSM00102. ADF. 1 hit.
[Graphical view]
PROSITEiPS51263. ADF_H. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Crossbred X Angus.
    Tissue: Ileum.

Entry informationi

Entry nameiCOF1_BOVIN
AccessioniPrimary (citable) accession number: Q5E9F7
Secondary accession number(s): Q3SZ74
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: January 23, 2007
Last modified: May 27, 2015
This is version 79 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.