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Q5E9F7

- COF1_BOVIN

UniProt

Q5E9F7 - COF1_BOVIN

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Protein

Cofilin-1

Gene

CFL1

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity. Regulates actin cytoskeleton dynamics. Important for normal progress through mitosis and normal cytokinesis. Plays a role in the regulation of cell morphology and cytoskeletal organization (By similarity). Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation (By similarity).By similarity

GO - Biological processi

  1. actin filament depolymerization Source: InterPro
  2. cytoskeleton organization Source: UniProtKB
  3. establishment of cell polarity Source: Ensembl
  4. mitotic cytokinesis Source: Ensembl
  5. neural crest cell migration Source: Ensembl
  6. neural fold formation Source: Ensembl
  7. positive regulation of actin filament depolymerization Source: Ensembl
  8. protein phosphorylation Source: Ensembl
  9. regulation of cell morphogenesis Source: UniProtKB
  10. response to amino acid Source: Ensembl
  11. response to virus Source: Ensembl
Complete GO annotation...

Keywords - Ligandi

Actin-binding

Enzyme and pathway databases

ReactomeiREACT_205196. Sema3A PAK dependent Axon repulsion.
REACT_211475. Regulation of actin dynamics for phagocytic cup formation.
REACT_259381. EPHB-mediated forward signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
Cofilin-1
Alternative name(s):
Cofilin, non-muscle isoform
Gene namesi
Name:CFL1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome 29

Subcellular locationi

Nucleus matrix By similarity. Cytoplasmcytoskeleton By similarity. Cell projectionruffle membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity. Cell projectionlamellipodium membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
Note: Colocalizes with the actin cytoskeleton in membrane ruffles and lamellipodia. Detected at the cleavage furrow and contractile ring during cytokinesis. Almost completely in nucleus in cells exposed to heat shock (By similarity).By similarity

GO - Cellular componenti

  1. cell-cell junction Source: Ensembl
  2. cell projection Source: UniProtKB-KW
  3. cortical actin cytoskeleton Source: Ensembl
  4. extracellular space Source: Ensembl
  5. extracellular vesicular exosome Source: Ensembl
  6. nucleus Source: UniProtKB-KW
  7. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 166165Cofilin-1PRO_0000214897Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei3 – 31PhosphoserineBy similarity
Modified residuei8 – 81PhosphoserineBy similarity
Modified residuei13 – 131N6-acetyllysineBy similarity
Modified residuei25 – 251PhosphothreonineBy similarity
Modified residuei68 – 681PhosphotyrosineBy similarity
Modified residuei73 – 731N6-acetyllysineBy similarity
Modified residuei140 – 1401PhosphotyrosineBy similarity
Modified residuei144 – 1441N6-acetyllysineBy similarity
Modified residuei156 – 1561PhosphoserineBy similarity

Post-translational modificationi

Inactivated by phosphorylation on Ser-3. Phosphorylated on Ser-3 in resting cells (By similarity). Dephosphorylated by PDXP/chronophin; this restores its activity in promoting actin filament depolymerization. The phosphorylation of Ser-24 may prevent recognition of the nuclear localization signal (By similarity). Phosphorylated via a ARRB1-RAC1-LIMK1-PAK1 cascade upon active ligand stimulation of atypical chemokine receptor ACKR2 (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ5E9F7.
PRIDEiQ5E9F7.

Expressioni

Gene expression databases

ExpressionAtlasiQ5E9F7. baseline.

Interactioni

Subunit structurei

Can bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is a major component of intranuclear and cytoplasmic actin rods (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliQ5E9F7.
SMRiQ5E9F7. Positions 1-166.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 153150ADF-HPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi30 – 345Nuclear localization signalSequence Analysis

Sequence similaritiesi

Belongs to the actin-binding proteins ADF family.Curated
Contains 1 ADF-H domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG286948.
GeneTreeiENSGT00440000033289.
HOVERGENiHBG000381.
InParanoidiQ5E9F7.
KOiK05765.
OMAiSKQIHTM.
OrthoDBiEOG7353Z9.
TreeFamiTF328601.

Family and domain databases

Gene3Di3.40.20.10. 1 hit.
InterProiIPR002108. ADF-H.
IPR029006. ADF-H/Gelsolin-like_dom.
IPR017904. ADF/Cofilin/Destrin.
IPR027234. Cofilin_1.
[Graphical view]
PANTHERiPTHR11913. PTHR11913. 1 hit.
PTHR11913:SF17. PTHR11913:SF17. 1 hit.
PfamiPF00241. Cofilin_ADF. 1 hit.
[Graphical view]
PRINTSiPR00006. COFILIN.
SMARTiSM00102. ADF. 1 hit.
[Graphical view]
PROSITEiPS51263. ADF_H. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5E9F7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MASGVAVSDG VIKVFNDMKV RKSSTPEEVK KRKKAVLFCL SEDKKNIILE
60 70 80 90 100
EGKEILVGDV GQTVDDPYAT FVKMLPDKDC RYALYDATYE TKESKKEDLV
110 120 130 140 150
FIFWAPECAP LKSKMIYASS KDAIKKKLTG IKHELQANCY EEVKDRCTLA
160
EKLGGSAVIS LEGKPL
Length:166
Mass (Da):18,519
Last modified:January 23, 2007 - v3
Checksum:i589EE8EC1ED12719
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT020963 mRNA. Translation: AAX08980.1.
BC103077 mRNA. Translation: AAI03078.1.
RefSeqiNP_001015655.1. NM_001015655.1.
UniGeneiBt.52428.

Genome annotation databases

EnsembliENSBTAT00000028602; ENSBTAP00000028602; ENSBTAG00000021455.
GeneIDi534553.
KEGGibta:534553.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT020963 mRNA. Translation: AAX08980.1 .
BC103077 mRNA. Translation: AAI03078.1 .
RefSeqi NP_001015655.1. NM_001015655.1.
UniGenei Bt.52428.

3D structure databases

ProteinModelPortali Q5E9F7.
SMRi Q5E9F7. Positions 1-166.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PaxDbi Q5E9F7.
PRIDEi Q5E9F7.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSBTAT00000028602 ; ENSBTAP00000028602 ; ENSBTAG00000021455 .
GeneIDi 534553.
KEGGi bta:534553.

Organism-specific databases

CTDi 1072.

Phylogenomic databases

eggNOGi NOG286948.
GeneTreei ENSGT00440000033289.
HOVERGENi HBG000381.
InParanoidi Q5E9F7.
KOi K05765.
OMAi SKQIHTM.
OrthoDBi EOG7353Z9.
TreeFami TF328601.

Enzyme and pathway databases

Reactomei REACT_205196. Sema3A PAK dependent Axon repulsion.
REACT_211475. Regulation of actin dynamics for phagocytic cup formation.
REACT_259381. EPHB-mediated forward signaling.

Miscellaneous databases

NextBioi 20876442.

Gene expression databases

ExpressionAtlasi Q5E9F7. baseline.

Family and domain databases

Gene3Di 3.40.20.10. 1 hit.
InterProi IPR002108. ADF-H.
IPR029006. ADF-H/Gelsolin-like_dom.
IPR017904. ADF/Cofilin/Destrin.
IPR027234. Cofilin_1.
[Graphical view ]
PANTHERi PTHR11913. PTHR11913. 1 hit.
PTHR11913:SF17. PTHR11913:SF17. 1 hit.
Pfami PF00241. Cofilin_ADF. 1 hit.
[Graphical view ]
PRINTSi PR00006. COFILIN.
SMARTi SM00102. ADF. 1 hit.
[Graphical view ]
PROSITEi PS51263. ADF_H. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Crossbred X Angus.
    Tissue: Ileum.

Entry informationi

Entry nameiCOF1_BOVIN
AccessioniPrimary (citable) accession number: Q5E9F7
Secondary accession number(s): Q3SZ74
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: January 23, 2007
Last modified: November 26, 2014
This is version 75 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3