Reviewed, UniProtKB/Swiss-Prot Q5E9F2 (HMOX1_BOVIN)

Names and origin

Protein names

Recommended name:
    Heme oxygenase 1
      Short name=HO-1
    EC=1.14.99.3

Gene names

Name:

HMOX1

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	289 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed By similarity.
Catalytic activity	Heme + 3 AH2 + 3 O2 = biliverdin + Fe2+ + CO + 3 A + 3 H2O.
Subcellular location	Microsome By similarity. Endoplasmic reticulum By similarity.
Sequence similarities	Belongs to the heme oxygenase family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Microsome
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	heme oxidation Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-SubCell microsome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	heme oxygenase (decyclizing) activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 289	289	Heme oxygenase 1		PRO_0000209686
Sites
	Metal binding	26	1	Iron (heme axial ligand) By similarity
Experimental info
	Sequence conflict	28	1	Q → E AA sequence Ref.3
	Sequence conflict	100	1	P → PH AA sequence Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q5E9F2-1 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: 8EAEA06BCCA4B96C	FASTA	289	32,940
	10 20 30 40 50 60 MERPQPDSSM PQDLSEALKE ATKEVHTQAE NAEFMKNFQK GELTQEGFKL VMASLYHIYV 70 80 90 100 110 120 ALEEEIERNK ENPVYTPLYF PEELHRRASL EQDMAFWYGP RWQEAIPYTQ ATKRYVQRLQ 130 140 150 160 170 180 EVGRTEPELL VAHAYTRYLG DLSGGQVLKK IAQKALNLPS SGEGLAFFTF PNIASATKFK 190 200 210 220 230 240 QLYRSRMNTL EMTPEVRQRV LDEAKTAFLL NIQLFEELQG LLTQKAKDHD PLQAPELHRR 250 260 270 280 AGSKVQDLAP TKASRGKPQP SVLSQAPLLR WVLTLSFLVA TVAVGLYAM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of 954 bovine full-CDS cDNA sequences." Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L. BMC Genomics 6:166-166(2005) [PubMed: 16305752] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[2]	NIH - Mammalian Gene Collection (MGC) project Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Crossbred X Angus. Tissue: Ileum.
[3]	"Structural studies on bovine spleen heme oxygenase. Immunological and structural diversity among mammalian heme oxygenase enzymes." Schacter B.A., Cripps V., Troxler R.F., Offner G.D. Arch. Biochem. Biophys. 282:404-412(1990) [PubMed: 1700666] [Abstract] Cited for: PROTEIN SEQUENCE OF 24-33; 41-49; 88-113; 138-147; 155-158; 187-196 AND 200-205. Tissue: Spleen.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BT020968 mRNA. Translation: AAX08985.1. BC102105 mRNA. Translation: AAI02106.4.
IPI	IPI00690186.
PIR	S13265.
RefSeq	NP_001014912.1.
UniGene	Bt.4001
3D structure databases
SMR	Q5E9F2. Positions 1-224.
ModBase	Search...
Genome annotation databases
Ensembl	ENSBTAG00000015582. Bos taurus. [Contig view]
GeneID	513221.
KEGG	bta:513221.
Phylogenomic databases
HOVERGEN	Q5E9F2.
Enzyme and pathway databases
BRENDA	1.14.99.3. 251.
Family and domain databases
InterPro	IPR002051. Haem_Oase. IPR016053. Haem_Oase-like. IPR016084. Haem_Oase-like_multi-hlx. IPR018207. Haem_oxygenase_CS. [Graphical view]
Gene3D	G3DSA:1.20.910.10. Haem_Oase-like_multi-hlx. 1 hit.
PANTHER	PTHR10720. Haem_Oase. 1 hit.
Pfam	PF01126. Heme_oxygenase. 1 hit. [Graphical view]
PIRSF	PIRSF000343. Haem_Oase. 1 hit.
PRINTS	PR00088. HAEMOXYGNASE.
PROSITE	PS00593. HEME_OXYGENASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HMOX1_BOVIN

Accession

Primary (citable) accession number: Q5E9F2
Secondary accession number(s): Q3ZCK7, Q7M338

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	March 15, 2005
Last modified:	June 16, 2009
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)