Q5E9C1 (CASP4_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Caspase-4
Short name=CASP-4
EC=3.4.22.57

Cleaved into the following 2 chains:

1. Caspase-4 subunit 1
2. Caspase-4 subunit 2

Gene names

Name:

CASP4

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	377 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves caspase-1 By similarity.
Catalytic activity	Strict requirement for Asp at the P1 position. It has a preferred cleavage sequence of Tyr-Val-Ala-Asp-|- but also cleaves at Asp-Glu-Val-Asp-|-.
Subunit structure	Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a small and a large subunit By similarity.
Post-translational modification	The two subunits are derived from the precursor sequence by an autocatalytic mechanism or by cleavage by Caspase-8 By similarity.
Sequence similarities	Belongs to the peptidase C14A family. Contains 1 CARD domain.

Ontologies

Keywords
Biological process	Apoptosis
Molecular function	Hydrolase Protease Thiol protease
PTM	Phosphoprotein Zymogen
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	apoptotic process Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular function	cysteine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Propeptide	1 – ?80	80	Potential		PRO_0000244735
	Chain	?81 – 270	190	Caspase-4 subunit 1		PRO_0000244736
	Propeptide	271 – 289	19	Potential		PRO_0000244737
	Chain	290 – 377	88	Caspase-4 subunit 2		PRO_0000244738
Regions
	Domain	1 – 91	91	CARD
Sites
	Active site	210	1	By similarity
	Active site	258	1	By similarity
Amino acid modifications
	Modified residue	83	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5E9C1 [UniParc]. Last modified May 10, 2005. Version 1. Checksum: 959A59424DAECBF4	FASTA	377	43,012
	10 20 30 40 50 60 MAEDKHNKNP LKMLESLGKE LISGLLDDFV EKNVLKLEEE EKKKIYDAKL QDKARVLVDS 70 80 90 100 110 120 IRQKNQEAGQ VFVQTFLNID KNSTSIKAPE ETVAGPDESV GSAATLKLCP HEEFLKLCKE 130 140 150 160 170 180 RAGEIYPIKE RKDRTRLALI ICNTEFDHMP PRNGAALDIL GMKQLLEGLG YTVEVEEKLT 190 200 210 220 230 240 ARDMESVLWK FAAREEHKSS DSTFLVFMSH GILDGICGTM HSEEEPDVLP YDTIFRTFNN 250 260 270 280 290 300 RNCLSLKDKP KVIIVQACRG ANRGELWVSD SPPALADSFS QSSENLEEDA VYKTHVEKDF 310 320 330 340 350 360 IAFCSSTPHN VSWRDIKKGS LFITRLITCF QKYAWCCHLE EVFRKVQQSF EKPNVKAQMP 370 TVERLSMTRY FYLFPGN

References

[1]	"Characterization of 954 bovine full-CDS cDNA sequences." Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L. BMC Genomics 6:166-166(2005) [PubMed: 16305752] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[2]	NIH - Mammalian Gene Collection (MGC) project Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Hypothalamus.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BT020999 mRNA. Translation: AAX09016.1. BC112708 mRNA. Translation: AAI12709.1.
IPI	IPI00686191.
RefSeq	NP_788811.1. NM_176638.4.
UniGene	Bt.16018.
3D structure databases
ProteinModelPortal	Q5E9C1.
SMR	Q5E9C1. Positions 124-270, 290-377.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5E9C1.
Proteomic databases
PRIDE	Q5E9C1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000027820; ENSBTAP00000027820; ENSBTAG00000020884.
GeneID	338039.
KEGG	bta:338039.
Organism-specific databases
CTD	837.
Phylogenomic databases
GeneTree	ENSGT00560000076835.
HOVERGEN	HBG076981.
InParanoid	Q5E9C1.
OrthoDB	EOG49W2FS.
PhylomeDB	Q5E9C1.
Family and domain databases
InterPro	IPR001315. CARD. IPR017350. Caspase_IL-1_beta. IPR011029. DEATH-like. IPR011600. Pept_C14_cat. IPR001309. Pept_C14_ICE_p20. IPR016129. Pept_C14_ICE_p20_AS. IPR002138. Pept_C14_p10. IPR002398. Pept_C14_p45. IPR015917. Pept_C14_p45_core. [Graphical view]
Gene3D	G3DSA:1.10.533.10. DEATH_like. 1 hit.
KO	K04394.
PANTHER	PTHR10454. Pept_C14_p45. 1 hit.
Pfam	PF00619. CARD. 1 hit. PF00656. Peptidase_C14. 1 hit. [Graphical view]
PIRSF	PIRSF038001. Caspase_ICE. 1 hit.
PRINTS	PR00376. IL1BCENZYME.
SMART	SM00114. CARD. 1 hit. SM00115. CASc. 1 hit. [Graphical view]
SUPFAM	SSF47986. DEATH_like. 1 hit.
PROSITE	PS50209. CARD. 1 hit. PS01122. CASPASE_CYS. 1 hit. PS01121. CASPASE_HIS. 1 hit. PS50207. CASPASE_P10. 1 hit. PS50208. CASPASE_P20. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CASP4_BOVIN

Accession

Primary (citable) accession number: Q5E9C1

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 27, 2006
Last sequence update:	May 10, 2005
Last modified:	November 16, 2011
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program