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Protein

Geranylgeranyl transferase type-2 subunit beta

Gene

RABGGTB

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the transfer of a geranylgeranyl moiety from geranylgeranyl diphosphate to both cysteines of Rab proteins with the C-terminal sequence -XXCC, -XCXC and -CCXX, such as RAB1A, RAB3A, RAB5A and RAB7A.By similarity

Catalytic activityi

Geranylgeranyl diphosphate + protein-cysteine = S-geranylgeranyl-protein + diphosphate.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Enzyme regulationi

The enzymatic reaction requires the aid of a Rab escort protein (also called component A), such as CHM.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi238Zinc; catalyticBy similarity1
Metal bindingi240Zinc; catalyticBy similarity1
Metal bindingi290Zinc; via tele nitrogen; catalyticBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Prenyltransferase, Transferase

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Geranylgeranyl transferase type-2 subunit beta (EC:2.5.1.60)
Alternative name(s):
Geranylgeranyl transferase type II subunit beta
Short name:
GGTase-II-beta
Rab geranyl-geranyltransferase subunit beta
Short name:
Rab GG transferase beta
Short name:
Rab GGTase beta
Rab geranylgeranyltransferase subunit beta
Type II protein geranyl-geranyltransferase subunit beta
Gene namesi
Name:RABGGTB
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002444332 – 331Geranylgeranyl transferase type-2 subunit betaAdd BLAST330

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylglycineBy similarity1
Modified residuei3PhosphothreonineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ5E9B3.
PRIDEiQ5E9B3.

Interactioni

Subunit structurei

Heterotrimer composed of RABGGTA, RABGGTB and CHM; within this trimer, RABGGTA and RABGGTB form the catalytic component B, while CHM (component A) mediates peptide substrate binding. The Rab GGTase dimer (RGGT) interacts with CHM (component A) prior to Rab protein binding; the association is stabilized by geranylgeranyl pyrophosphate (GGpp). The CHM:RGGT:Rab complex is destabilized by GGpp. Interaction of RABGGTB with prenylated PTP4A2 precludes its association with RABGGTA and inhibits enzyme activity (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000024551.

Structurei

3D structure databases

ProteinModelPortaliQ5E9B3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati20 – 61PFTB 1Add BLAST42
Repeati68 – 109PFTB 2Add BLAST42
Repeati116 – 157PFTB 3Add BLAST42
Repeati164 – 205PFTB 4Add BLAST42
Repeati212 – 253PFTB 5Add BLAST42
Repeati260 – 302PFTB 6Add BLAST43

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni190 – 192Geranylgeranyl diphosphate bindingBy similarity3
Regioni232 – 244Geranylgeranyl diphosphate bindingBy similarityAdd BLAST13

Sequence similaritiesi

Contains 6 PFTB repeats.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0366. Eukaryota.
COG5029. LUCA.
HOGENOMiHOG000180334.
HOVERGENiHBG008182.
InParanoidiQ5E9B3.
KOiK05956.

Family and domain databases

CDDicd02894. GGTase-II. 1 hit.
Gene3Di1.50.10.20. 1 hit.
InterProiIPR001330. PFTB_repeat.
IPR026873. Ptb1.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
[Graphical view]
PANTHERiPTHR11774:SF11. PTHR11774:SF11. 1 hit.
PfamiPF00432. Prenyltrans. 5 hits.
[Graphical view]
SUPFAMiSSF48239. SSF48239. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5E9B3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGTPQKDVII KSDAPDTLLL EKHADYIASY GSKKDDYEYC MSEYLRMSGI
60 70 80 90 100
YWGLTVMDLM GQLHRMNREE ILTFIKSCQH ECGGISASIG HDPHLLYTLS
110 120 130 140 150
AVQILTLYDS INVIDINKVV EYVQSLQKED GSFAGDIWGE IDTRFSFCAV
160 170 180 190 200
ATLALLGKLD AINVEKAIEF VLSCMNFDGG FGCRPGSESH AGQIYCCTGF
210 220 230 240 250
LAITSQLHQV NSDLLGWWLC ERQLPSGGLN GRPEKLPDVC YSWWVLASLK
260 270 280 290 300
IIGRLHWIDR EKLRSFILAC QDEETGGFAD RPGDMVDPFH TLFGIAGLSL
310 320 330
LGEEQIKPVS PVFCMPEEVL RRVNVQPELV S
Length:331
Mass (Da):36,972
Last modified:March 15, 2005 - v1
Checksum:iCB554CA9B22D334A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT021007 mRNA. Translation: AAX09024.1.
BC147953 mRNA. Translation: AAI47954.1.
RefSeqiNP_001015646.1. NM_001015646.1.
UniGeneiBt.20263.

Genome annotation databases

GeneIDi533276.
KEGGibta:533276.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT021007 mRNA. Translation: AAX09024.1.
BC147953 mRNA. Translation: AAI47954.1.
RefSeqiNP_001015646.1. NM_001015646.1.
UniGeneiBt.20263.

3D structure databases

ProteinModelPortaliQ5E9B3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000024551.

Proteomic databases

PaxDbiQ5E9B3.
PRIDEiQ5E9B3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi533276.
KEGGibta:533276.

Organism-specific databases

CTDi5876.

Phylogenomic databases

eggNOGiKOG0366. Eukaryota.
COG5029. LUCA.
HOGENOMiHOG000180334.
HOVERGENiHBG008182.
InParanoidiQ5E9B3.
KOiK05956.

Family and domain databases

CDDicd02894. GGTase-II. 1 hit.
Gene3Di1.50.10.20. 1 hit.
InterProiIPR001330. PFTB_repeat.
IPR026873. Ptb1.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
[Graphical view]
PANTHERiPTHR11774:SF11. PTHR11774:SF11. 1 hit.
PfamiPF00432. Prenyltrans. 5 hits.
[Graphical view]
SUPFAMiSSF48239. SSF48239. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPGTB2_BOVIN
AccessioniPrimary (citable) accession number: Q5E9B3
Secondary accession number(s): A6QLG2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: March 15, 2005
Last modified: October 5, 2016
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.