Reviewed, UniProtKB/Swiss-Prot Q5E9B3 (PGTB2_BOVIN)

Names and origin

Protein names

Recommended name:
    Geranylgeranyl transferase type-2 subunit beta
    EC=2.5.1.60
Alternative name(s):
    Geranylgeranyl transferase type II subunit beta
      Short name=GGTase-II-beta
    Type II protein geranyl-geranyltransferase subunit beta
    Rab geranylgeranyltransferase subunit beta
    Rab geranyl-geranyltransferase subunit beta
      Short name=Rab GG transferase beta
      Short name=Rab GGTase beta

Gene names

Name:

RABGGTB

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	331 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the transfer of a geranyl-geranyl moiety from geranyl-geranyl pyrophosphate to both cysteines in Rab proteins with an -XXCC, -XCXC and -CCXX C-terminal, such as RAB1A, RAB3A and RAB5A respectively By similarity.
Catalytic activity	Geranylgeranyl diphosphate + protein-cysteine = S-geranylgeranyl-protein + diphosphate.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Enzyme regulation	The enzymatic reaction requires the aid of a Rab escort protein (also called component A) By similarity.
Subunit structure	Heterodimer of an alpha and a beta subunit, collectively called component B. Interaction of beta subunit with prenylated PTP4A2 precludes its association with subunit alpha, and inhibits enzyme activity By similarity.
Sequence similarities	Belongs to the protein prenyltransferase subunit beta family. Contains 6 PFTB repeats.

Ontologies

Keywords
Domain	Repeat
Ligand	Metal-binding Zinc
Molecular function	Prenyltransferase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Molecular function	Rab geranylgeranyltransferase activity Inferred from electronic annotation. Source: EC protein binding Inferred from sequence or structural similarity. Source: AgBase zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 331	331	Geranylgeranyl transferase type-2 subunit beta		PRO_0000244433
Regions
	Repeat	20 – 61	42	PFTB 1
	Repeat	68 – 109	42	PFTB 2
	Repeat	116 – 157	42	PFTB 3
	Repeat	164 – 205	42	PFTB 4
	Repeat	212 – 253	42	PFTB 5
	Repeat	260 – 302	43	PFTB 6
Sites
	Metal binding	238	1	Zinc By similarity
	Metal binding	240	1	Zinc By similarity
	Metal binding	290	1	Zinc By similarity
Amino acid modifications
	Modified residue	3	1	Phosphothreonine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5E9B3-1 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: CB554CA9B22D334A	FASTA	331	36,972
	10 20 30 40 50 60 MGTPQKDVII KSDAPDTLLL EKHADYIASY GSKKDDYEYC MSEYLRMSGI YWGLTVMDLM 70 80 90 100 110 120 GQLHRMNREE ILTFIKSCQH ECGGISASIG HDPHLLYTLS AVQILTLYDS INVIDINKVV 130 140 150 160 170 180 EYVQSLQKED GSFAGDIWGE IDTRFSFCAV ATLALLGKLD AINVEKAIEF VLSCMNFDGG 190 200 210 220 230 240 FGCRPGSESH AGQIYCCTGF LAITSQLHQV NSDLLGWWLC ERQLPSGGLN GRPEKLPDVC 250 260 270 280 290 300 YSWWVLASLK IIGRLHWIDR EKLRSFILAC QDEETGGFAD RPGDMVDPFH TLFGIAGLSL 310 320 330 LGEEQIKPVS PVFCMPEEVL RRVNVQPELV S

References

[1]	"Characterization of 954 bovine full-CDS cDNA sequences." Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L. BMC Genomics 6:166-166(2005) [PubMed: 16305752] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[2]	NIH - Mammalian Gene Collection (MGC) project Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Fetal medulla.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BT021007 mRNA. Translation: AAX09024.1. BC147953 mRNA. Translation: AAI47954.1.
IPI	IPI00705500.
RefSeq	NP_001015646.1.
UniGene	Bt.20263
3D structure databases
SMR	Q5E9B3. Positions 3-331.
ModBase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000024551; ENSBTAP00000024551; ENSBTAG00000018447; Bos taurus. [Genome view]
GeneID	533276.
KEGG	bta:533276.
Organism-specific databases
CTD	533276.
Phylogenomic databases
HOVERGEN	Q5E9B3.
OMA	YWGLTAL.
Enzyme and pathway databases
BRENDA	2.5.1.60. 251.
Family and domain databases
InterPro	IPR001330. Prenyltrans. [Graphical view]
Pfam	PF00432. Prenyltrans. 5 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PGTB2_BOVIN

Accession

Primary (citable) accession number: Q5E9B3
Secondary accession number(s): A6QLG2

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 27, 2006
Last sequence update:	March 15, 2005
Last modified:	November 3, 2009
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)