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Protein

L-lactate dehydrogenase B chain

Gene

LDHB

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

(S)-lactate + NAD+ = pyruvate + NADH.

Pathwayi: pyruvate fermentation to lactate

This protein is involved in step 1 of the subpathway that synthesizes (S)-lactate from pyruvate.
Proteins known to be involved in this subpathway in this organism are:
  1. L-lactate dehydrogenase A-like 6B (LDHAL6B), L-lactate dehydrogenase A chain (LDHA), L-lactate dehydrogenase B chain (LDHB), L-lactate dehydrogenase (LDHB), L-lactate dehydrogenase (LDHC), L-lactate dehydrogenase (LDHC), L-lactate dehydrogenase (LDHC)
This subpathway is part of the pathway pyruvate fermentation to lactate, which is itself part of Fermentation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-lactate from pyruvate, the pathway pyruvate fermentation to lactate and in Fermentation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei100 – 1001NADBy similarity
Binding sitei107 – 1071SubstrateBy similarity
Binding sitei139 – 1391NAD or substrateBy similarity
Binding sitei170 – 1701SubstrateBy similarity
Active sitei194 – 1941Proton acceptorBy similarity
Binding sitei249 – 2491SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi53 – 586NADBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Enzyme and pathway databases

ReactomeiR-BTA-70268. Pyruvate metabolism.
SABIO-RKQ5E9B1.
UniPathwayiUPA00554; UER00611.

Names & Taxonomyi

Protein namesi
Recommended name:
L-lactate dehydrogenase B chain (EC:1.1.1.27)
Short name:
LDH-B
Gene namesi
Name:LDHB
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 5

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 334333L-lactate dehydrogenase B chainPRO_0000239139Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei5 – 51N6-acetyllysineBy similarity
Modified residuei7 – 71N6-acetyllysineBy similarity
Modified residuei44 – 441PhosphoserineBy similarity
Modified residuei58 – 581N6-acetyllysineBy similarity
Modified residuei82 – 821N6-acetyllysineBy similarity
Modified residuei119 – 1191N6-acetyllysineBy similarity
Modified residuei127 – 1271N6-acetyllysineBy similarity
Modified residuei240 – 2401PhosphotyrosineBy similarity
Modified residuei319 – 3191N6-acetyllysineBy similarity
Modified residuei329 – 3291N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ5E9B1.
PeptideAtlasiQ5E9B1.
PRIDEiQ5E9B1.

Expressioni

Gene expression databases

BgeeiENSBTAG00000019603.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000026118.

Chemistry

BindingDBiQ5E9B1.

Structurei

3D structure databases

ProteinModelPortaliQ5E9B1.
SMRiQ5E9B1. Positions 2-333.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. LDH family.Curated

Phylogenomic databases

eggNOGiKOG1495. Eukaryota.
COG0039. LUCA.
GeneTreeiENSGT00550000074541.
HOGENOMiHOG000213793.
HOVERGENiHBG000462.
InParanoidiQ5E9B1.
KOiK00016.
OMAiCNDKLQG.
OrthoDBiEOG091G0HME.
TreeFamiTF314963.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00488. Lactate_dehydrog. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR011304. L-lactate_DH.
IPR018177. L-lactate_DH_AS.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01771. L-LDH-NAD. 1 hit.
PROSITEiPS00064. L_LDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5E9B1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATLKEKLIA PVAEEETRIP NNKITVVGVG QVGMACAISI LGKSLTDELA
60 70 80 90 100
LVDVLEDKLK GEMMDLQHGS LFLQTPKIVA DKDYSVTANS KIVVVTAGVR
110 120 130 140 150
QQEGESRLNL VQRNVNVFKF IIPQIVKYSP DCIIIVVSNP VDILTYVTWK
160 170 180 190 200
LSGLPKHRVI GSGCNLDSAR FRYLMAEKLG IHPSSCHGWI LGEHGDSSVA
210 220 230 240 250
VWSGVNVAGV SLQELNPEMG TDNDSENWKE VHKMVVESAY EVIKLKGYTN
260 270 280 290 300
WAIGLSVADL IESMLKNLSR IHPVSTMVKG MYGIENEVFL SLPCILNARG
310 320 330
LTSVINQKLK DEEVAQLKKS ADTLWGIQKD LKDL
Length:334
Mass (Da):36,724
Last modified:January 23, 2007 - v4
Checksum:i95E17649F7C162B1
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti8 – 81L → S in AAX09026 (PubMed:16305752).Curated
Sequence conflicti18 – 181R → T in CAB96751 (Ref. 1) Curated
Sequence conflicti131 – 1311D → A in CAB96751 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ401268 mRNA. Translation: CAB96751.1.
BT021009 mRNA. Translation: AAX09026.1.
BC102217 mRNA. Translation: AAI02218.1.
BC142006 mRNA. Translation: AAI42007.1.
RefSeqiNP_001303267.1. NM_001316338.1.
NP_776525.2. NM_174100.2.
UniGeneiBt.7736.

Genome annotation databases

EnsembliENSBTAT00000026118; ENSBTAP00000026118; ENSBTAG00000019603.
GeneIDi281275.
KEGGibta:281275.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ401268 mRNA. Translation: CAB96751.1.
BT021009 mRNA. Translation: AAX09026.1.
BC102217 mRNA. Translation: AAI02218.1.
BC142006 mRNA. Translation: AAI42007.1.
RefSeqiNP_001303267.1. NM_001316338.1.
NP_776525.2. NM_174100.2.
UniGeneiBt.7736.

3D structure databases

ProteinModelPortaliQ5E9B1.
SMRiQ5E9B1. Positions 2-333.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000026118.

Chemistry

BindingDBiQ5E9B1.

Proteomic databases

PaxDbiQ5E9B1.
PeptideAtlasiQ5E9B1.
PRIDEiQ5E9B1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000026118; ENSBTAP00000026118; ENSBTAG00000019603.
GeneIDi281275.
KEGGibta:281275.

Organism-specific databases

CTDi3945.

Phylogenomic databases

eggNOGiKOG1495. Eukaryota.
COG0039. LUCA.
GeneTreeiENSGT00550000074541.
HOGENOMiHOG000213793.
HOVERGENiHBG000462.
InParanoidiQ5E9B1.
KOiK00016.
OMAiCNDKLQG.
OrthoDBiEOG091G0HME.
TreeFamiTF314963.

Enzyme and pathway databases

UniPathwayiUPA00554; UER00611.
ReactomeiR-BTA-70268. Pyruvate metabolism.
SABIO-RKQ5E9B1.

Gene expression databases

BgeeiENSBTAG00000019603.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00488. Lactate_dehydrog. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR011304. L-lactate_DH.
IPR018177. L-lactate_DH_AS.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01771. L-LDH-NAD. 1 hit.
PROSITEiPS00064. L_LDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLDHB_BOVIN
AccessioniPrimary (citable) accession number: Q5E9B1
Secondary accession number(s): A5PJ87, Q3T0X4, Q9MYV5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 98 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.